RLG00000009870

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010554
Physical Location & Seq
Forward (+)
59224201 .. 59226381
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009870

Sequence Viewer

Length: 969 bp
ATGTACGCCAGAGAAGAACTGCCTGGAGAAGGGGTTGAAGTGATTGCGGCTGATGCTCCCTCGGAGTACCAGATGGCTCCTAGGGCGGTGGAGCATAAGGACAAGGCGGCTGCTGGTTCAGGAGAGGGGACGGGTGAGCCAAAGAAGAGAGGGAGAGGGAGGCCTCCACAGAAGAATGTGGGGGAAGGGCAGTCCTCCTCAGCCAAGGGGAGAAAGGAGAAGGGCCAAGGAAGCGGTGGTGGGGGTGGAGGTGCCGGTGGCAGTGGCAGTGGGGGCGGAGGAGGAGGAGGAGGAGGAGGAGGCGGTGGGGGCGGGGGCGGCGGCCAGGGTGAGTACTTAACCATGTTTGAGTTTACTAATTTGAATTGTATTGTAGCCTTATGGAATGAGAATGGCAATCCCGGTGCAGCTTATAATGGAAGAAGCTTCACGCCCCACCTCATCTCTGTCAATGCCGGGGAGAATGTCTGCCGGAGTGTTATGAGATATTTCCATGAAAGACCACGAGTAATCTGCGTGATATCTGCAAGTGGAGTAATCTCTAGTGTTAAACTGCGCCAACAGGATGACGAGGCCATCACATATGAGGGACGTTTTGAAATCCTGAGCTTGTCTGGATGCCTTATGCCCCATAACGGGGCCTGGCGTGGTGCAATGAGTGTCTCTCTGGCAGGCGGCAACGGTCTAATTTTAGGGGGAGGCATTAATGGAATTCTGAGAGCTGCCACTCCTGTGCAAGTTGTGGTAGGTAGTTTCCTAGCTGGTGATCCGAAAGGAAAGGGTAAGAAGAAGGCGGATGCTGAACCACCTGATGCTGAGCCTCCACCTCCGCCACCGCCGTCGCCTCAGCCGCCAGCACCAGAACCGCCACCTGCACCAGGACCACCACCACCACCAGCACCTCCACCACAAGAGCCCATGGGATTCTGGGGGATATACGATAATAGCTGGCCGAGAATGGAATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

323

Amino Acids

32.73

Weight (kDa)

6.46

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 145 - 252 4.8e-20 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AarI CACCTGC 1 cut(s) 880
Acc36I ACCTGC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 251
AclWI GGATC 1 cut(s) 761
AcoI YGGCCR 2 cut(s) 322, 950
AcsI RAATTY 1 cut(s) 711
AfaI GTAC 3 cut(s) 5, 68, 335
AfiI CCNNNNNNNGG 5 cut(s) 29, 635, 636, 637, 878
AgsI TTSAA 3 cut(s) 38, 364, 599
AjnI CCWGG 4 cut(s) 22, 324, 641, 877
AleI CACNNNNGTG 1 cut(s) 731
AluBI AGCT 6 cut(s) 410, 426, 609, 722, 761, 948
AluI AGCT 6 cut(s) 410, 426, 609, 722, 761, 948
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 1 cut(s) 761
AoxI GGCC 6 cut(s) 161, 223, 322, 573, 639, 950
ApeKI GCWGC 3 cut(s) 110, 407, 722
ApoI RAATTY 1 cut(s) 711
AseI ATTAAT 1 cut(s) 705
AspA2I CCTAGG 1 cut(s) 80
AspLEI GCGC 1 cut(s) 558
AspS9I GGNCC 3 cut(s) 223, 639, 881
AsuC2I CCSGG 2 cut(s) 402, 457
AsuHPI GGTGA 3 cut(s) 146, 341, 776
AvaII GGWCC 1 cut(s) 881
AvrII CCTAGG 1 cut(s) 80
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 918
BauI CACGAG 1 cut(s) 504
BbvCI CCTCAGC 2 cut(s) 199, 846
BbvI GCAGC 3 cut(s) 97, 419, 709
BccI CCATC 2 cut(s) 67, 584
BceAI ACGGC 1 cut(s) 823
BcgI CGANNNNNNTGC 2 cut(s) 495, 529
BciT130I CCWGG 4 cut(s) 24, 326, 643, 879
BcnI CCSGG 2 cut(s) 402, 457
BcoDI GTCTC 1 cut(s) 667
BfaI CTAG 3 cut(s) 81, 543, 758
BfuAI ACCTGC 1 cut(s) 880
BisI GCNGC 9 cut(s) 48, 108, 111, 319, 322, 408, 676, 723, 851
BlnI CCTAGG 1 cut(s) 80
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 9 cut(s) 49, 109, 112, 320, 323, 409, 677, 724, 852
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 6 cut(s) 24, 326, 402, 457, 643, 879
Bme18I GGWCC 1 cut(s) 881
BmgT120I GGNCC 3 cut(s) 223, 639, 881
BmiI GGNNCC 3 cut(s) 78, 253, 640
BmrFI CCNGG 6 cut(s) 24, 326, 402, 457, 643, 879
BmsI GCATC 4 cut(s) 43, 608, 787, 802
BplI GAGNNNNNCTC 2 cut(s) 649, 681
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 3 cut(s) 199, 605, 846
Bpu1102I GCTNAGC 1 cut(s) 816
BpuMI CCSGG 2 cut(s) 402, 457
BsaJI CCNNGG 7 cut(s) 60, 80, 204, 226, 325, 456, 918
BsaXI ACNNNNNCTCC 2 cut(s) 18, 48
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 635, 636, 637, 878
Bse118I RCCGGY 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 660
BseBI CCWGG 4 cut(s) 24, 326, 643, 879
BseDI CCNNGG 7 cut(s) 60, 80, 204, 226, 325, 456, 918
BseGI GGATG 3 cut(s) 571, 623, 802
BseLI CCNNNNNNNGG 5 cut(s) 29, 635, 636, 637, 878
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 5 cut(s) 213, 596, 707, 807, 860
BseRI GAGGAG 8 cut(s) 187, 294, 297, 300, 303, 306, 309, 312
BseXI GCAGC 3 cut(s) 97, 419, 709
BsgI GTGCAG 2 cut(s) 426, 858
BshFI GGCC 6 cut(s) 163, 225, 324, 575, 641, 952
BshNI GGYRCC 1 cut(s) 251
BsiSI CCGG 4 cut(s) 255, 402, 456, 472
BslFI GGGAC 2 cut(s) 142, 603
BslI CCNNNNNNNGG 5 cut(s) 29, 635, 636, 637, 878
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 2 cut(s) 142, 603
BsnI GGCC 6 cut(s) 163, 225, 324, 575, 641, 952
Bsp1286I GDGCHC 1 cut(s) 918
Bsp143I GATC 1 cut(s) 766
Bsp1720I GCTNAGC 1 cut(s) 816
Bsp19I CCATGG 1 cut(s) 918
BspANI GGCC 6 cut(s) 163, 225, 324, 575, 641, 952
BspCNI CTCAG 5 cut(s) 212, 597, 708, 808, 859
BspLI GGNNCC 3 cut(s) 78, 253, 640
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 1 cut(s) 761
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 7 cut(s) 60, 80, 204, 226, 325, 456, 918
BssMI GATC 1 cut(s) 766
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 4 cut(s) 80, 204, 226, 918
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 4 cut(s) 24, 326, 643, 879
Bst4CI ACNGT 1 cut(s) 683
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 3 cut(s) 673, 855, 950
BstDEI CTNAG 5 cut(s) 199, 605, 716, 816, 846
BstDSI CCRYGG 1 cut(s) 918
BstENI CCTNNNNNAGG 2 cut(s) 27, 876
BstF5I GGATG 3 cut(s) 571, 623, 802
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 1 cut(s) 769
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 1 cut(s) 766
BstMWI GCNNNNNNNGC 7 cut(s) 53, 83, 231, 273, 309, 318, 850
BstNI CCWGG 4 cut(s) 24, 326, 643, 879
BstSCI CCNGG 6 cut(s) 22, 324, 400, 455, 641, 877
BstV1I GCAGC 3 cut(s) 97, 419, 709
BsuRI GGCC 6 cut(s) 163, 225, 324, 575, 641, 952
BtgI CCRYGG 1 cut(s) 918
BtsCI GGATG 3 cut(s) 571, 623, 802
BtsI GCAGTG 2 cut(s) 268, 274
BtsIMutI CAGTG 2 cut(s) 268, 274
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 3 cut(s) 673, 855, 950
CfoI GCGC 1 cut(s) 558
Cfr10I RCCGGY 1 cut(s) 254
Cfr13I GGNCC 3 cut(s) 223, 639, 881
Csp6I GTAC 3 cut(s) 4, 67, 334
CviAII CATG 3 cut(s) 343, 494, 919
CviQI GTAC 3 cut(s) 4, 67, 334
DdeI CTNAG 5 cut(s) 199, 605, 716, 816, 846
DpnI GATC 1 cut(s) 768
DpnII GATC 1 cut(s) 766
EaeI YGGCCR 2 cut(s) 322, 950
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EciI GGCGGA 3 cut(s) 291, 809, 819
Eco130I CCWWGG 4 cut(s) 80, 204, 226, 918
Eco147I AGGCCT 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 918
Eco32I GATATC 1 cut(s) 522
Eco47I GGWCC 1 cut(s) 881
EcoNI CCTNNNNNAGG 2 cut(s) 27, 876
EcoO109I RGGNCCY 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 4 cut(s) 22, 324, 641, 877
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 4 cut(s) 80, 204, 226, 918
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 4 cut(s) 80, 204, 226, 918
FaeI CATG 3 cut(s) 346, 497, 922
FaqI GGGAC 2 cut(s) 142, 603
FatI CATG 3 cut(s) 342, 493, 918
FauI CCCGC 1 cut(s) 305
FauNDI CATATG 1 cut(s) 583
Fnu4HI GCNGC 9 cut(s) 48, 108, 111, 319, 322, 408, 676, 723, 851
FokI GGATG 3 cut(s) 578, 630, 809
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 9 cut(s) 48, 108, 111, 319, 322, 408, 676, 723, 851
FspBI CTAG 3 cut(s) 81, 543, 758
GlaI GCGC 1 cut(s) 557
GluI GCNGC 9 cut(s) 48, 108, 111, 319, 322, 408, 676, 723, 851
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 6 cut(s) 163, 225, 324, 575, 641, 952
HapII CCGG 4 cut(s) 255, 402, 456, 472
HhaI GCGC 1 cut(s) 558
Hin1II CATG 3 cut(s) 346, 497, 922
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 424
HinfI GANTC 1 cut(s) 924
HpaII CCGG 4 cut(s) 255, 402, 456, 472
HphI GGTGA 3 cut(s) 146, 341, 776
Hpy166II GTNNAC 1 cut(s) 354
Hpy188I TCNGA 3 cut(s) 64, 717, 771
Hpy188III TCNNGA 3 cut(s) 120, 604, 615
Hpy8I GTNNAC 1 cut(s) 354
Hpy99I CGWCG 1 cut(s) 844
HpyAV CCTTC 4 cut(s) 23, 179, 214, 784
HpyCH4III ACNGT 1 cut(s) 683
HpyCH4IV ACGT 1 cut(s) 592
HpyCH4V TGCA 5 cut(s) 407, 527, 653, 736, 875
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 83, 231, 273, 309, 318, 850
HpyF3I CTNAG 5 cut(s) 199, 605, 716, 816, 846
HpySE526I ACGT 1 cut(s) 592
Hsp92II CATG 3 cut(s) 346, 497, 922
HspAI GCGC 1 cut(s) 556
Kzo9I GATC 1 cut(s) 766
LmnI GCTCC 3 cut(s) 61, 82, 91
Lsp1109I GCAGC 3 cut(s) 97, 419, 709
LweI GCATC 4 cut(s) 43, 608, 787, 802
MaeI CTAG 3 cut(s) 81, 543, 758
MaeII ACGT 1 cut(s) 592
MalI GATC 1 cut(s) 768
MboI GATC 1 cut(s) 766
MboII GAAGA 5 cut(s) 26, 157, 184, 432, 799
MhlI GDGCHC 1 cut(s) 918
MluCI AATT 4 cut(s) 358, 364, 687, 711
MseI TTAA 3 cut(s) 338, 549, 705
MslI CAYNNNNRTG 1 cut(s) 731
MspI CCGG 4 cut(s) 255, 402, 456, 472
MspR9I CCNGG 6 cut(s) 24, 326, 402, 457, 643, 879
MvaI CCWGG 4 cut(s) 24, 326, 643, 879
MwoI GCNNNNNNNGC 7 cut(s) 53, 83, 231, 273, 309, 318, 850
NciI CCSGG 2 cut(s) 402, 457
NcoI CCATGG 1 cut(s) 918
NdeI CATATG 1 cut(s) 583
NdeII GATC 1 cut(s) 766
NlaIII CATG 3 cut(s) 346, 497, 922
NlaIV GGNNCC 3 cut(s) 78, 253, 640
OliI CACNNNNGTG 1 cut(s) 731
PaqCI CACCTGC 1 cut(s) 880
PceI AGGCCT 1 cut(s) 163
PfeI GAWTC 1 cut(s) 924
PkrI GCNGC 9 cut(s) 49, 109, 112, 320, 323, 409, 677, 724, 852
PshBI ATTAAT 1 cut(s) 705
PsiI TTATAA 1 cut(s) 414
Psp6I CCWGG 4 cut(s) 22, 324, 641, 877
PspGI CCWGG 4 cut(s) 22, 324, 641, 877
PspN4I GGNNCC 3 cut(s) 78, 253, 640
PspPI GGNCC 3 cut(s) 223, 639, 881
RsaI GTAC 3 cut(s) 5, 68, 335
RsaNI GTAC 3 cut(s) 4, 67, 334
RseI CAYNNNNRTG 1 cut(s) 731
SaqAI TTAA 3 cut(s) 338, 549, 705
SatI GCNGC 9 cut(s) 48, 108, 111, 319, 322, 408, 676, 723, 851
Sau3AI GATC 1 cut(s) 766
Sau96I GGNCC 3 cut(s) 223, 639, 881
ScaI AGTACT 1 cut(s) 335
ScrFI CCNGG 6 cut(s) 24, 326, 402, 457, 643, 879
SduI GDGCHC 1 cut(s) 918
SfaNI GCATC 4 cut(s) 43, 608, 787, 802
SinI GGWCC 1 cut(s) 881
SmiMI CAYNNNNRTG 1 cut(s) 731
Sse9I AATT 4 cut(s) 358, 364, 687, 711
SseBI AGGCCT 1 cut(s) 163
SspMI CTAG 3 cut(s) 81, 543, 758
StuI AGGCCT 1 cut(s) 163
StyD4I CCNGG 6 cut(s) 22, 324, 400, 455, 641, 877
StyI CCWWGG 4 cut(s) 80, 204, 226, 918
TaaI ACNGT 1 cut(s) 683
TaiI ACGT 1 cut(s) 595
TasI AATT 4 cut(s) 358, 364, 687, 711
TatI WGTACW 1 cut(s) 333
TauI GCSGC 6 cut(s) 50, 110, 321, 324, 678, 853
TfiI GAWTC 1 cut(s) 924
Tru1I TTAA 3 cut(s) 338, 549, 705
Tru9I TTAA 3 cut(s) 338, 549, 705
TscAI CASTG 2 cut(s) 268, 274
TseI GCWGC 3 cut(s) 110, 407, 722
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 2 cut(s) 268, 274
VpaK11BI GGWCC 1 cut(s) 881
VspI ATTAAT 1 cut(s) 705
XagI CCTNNNNNAGG 2 cut(s) 27, 876
XapI RAATTY 1 cut(s) 711
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmaJI CCTAGG 1 cut(s) 80
XspI CTAG 3 cut(s) 81, 543, 758
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.