FvH4_4g11500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003234
Physical Location & Seq
Reverse (-)
15249349 .. 15255887
6539 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11500.t1

Sequence Viewer

Length: 2970 bp
ATGAGCAGAAGACACTTGAAGTCTCTGCTCTTGAACTCAATCTCCCCCTTCTCTTCTTCCTCCCCAAATCCAACAACAACTTCCCAATCCTTCCTTTCTTCTCTCAAATCCAAAACCCAAAACCTCAATGTCCTCTCCAGATTCCTCTCCGCCTCCTCTCCGCCTCCCACTCTCGACTTCTCCTCCCCGGAGACCCCCGAAGACTTTGCCTTTCTCCGCGACTCCCTCTCGGATTCCGCGCCGAAACTTCCGCCAGATAAGTGCTCTAACGACGCCGTTTTGATTTCCAATGCCATTAGGAATAGTACTGATGCATTTGGGGATGAAACCCAGAAGTTCCTTAGGAGGTTTAGGGATGATTTGAATGAGAGTTTGGTGGTGGAGGTTTTGAGTCTTGTGAAGAGTGCTGAGTTGGGTGTCAAGTTTTTCTTATGGGCTGGTAGACAAATTGGGTATAGTCACACTGGGTCTGTATATAATGCCTTGATTGAGTTACTGGAGCGTGGTAGTAGTAATGAGAGAGTGCCGGAACATTTTCTGCAAGAAATTAGGGGTGATGATAGAGAGGTGCTTGGGAAGTTGCTTAATGTGTTGATTCGCAAGTGTTGCCGCAATGGGCTGTGGAATGTGGTTTTGGAAGAGCTAGGGAGGCTTAAGGATTACGGGTACAGGCCGAGTCAGGCCACTTACAATGCTTTGGTTCAGGTGTTTTTGAGAGCTGATAGGCTGGACACGGCGCACTTGATTCATGGGGAGATGGTGGAGTTGGGGTTTAAGATGGATGAGTTTACGTTGGGTTGTTTCGGTCATGCGCTTTGTAAAGCTGGGAGGTGGAGGGAGGGGCTTGCGTTGATTGACAAGGAAGAGTTTGTGCCCAATACTGTTCTTTATACCAAGATGATATCAGGGTTGTGTGAAGCTTCACTCTTTGAGCAAGCTATGGATTTCTTGGGCAGAATGCGCTGTAATTCTTGCATTCCTAATGTCCTAACGTATAGGATTTTGCTGTGTGGATGTTTGAGGAAGAAACAGCTTGGCAGGTGCAAGAGAATTCTTAGTATGATGATCATGGAAGGTTGTTATCCTAGCCCTAGTATATTTAATTCTCTTGTTCATGCTTATTGCAGATCAGAAGATTACTCTTATGCTTATAAGTTGCTTAAAAAAATGGTAAAATGTCATTGCCAGCCAGGCTATGTGGTTTACAATATCTTGATTGGTGGTATCTGTGGAAACGAAGAATTACCTACCTTAGATATGTTAGACATGGCTGAAAAAGCTTATGGTGAGATGCTTAACGCAGGGGTTGTTCTAAATAAGGTAAATGTAAGCAATTTTGCCCGATGTCTTTGTGGCCATGGAAAATTTGACAAAGCTTACAAGGTCATCAATGAAATGATGAGCAAGGGTTTTGTTCCCGACACTAGTACATACTCTAAAGTTATTGGCTTATTGTGCAATGCCTCCAAGGTAGAACAAGCATTTTTGTTGTTTGAAGAAATGAAACAAAACGGTGTTGTTCCTGATGTCTACACATATACTATCCTAGTCGATAGTTTTTCTAAGGCTGGCCTTATTGAACAGGCTCAGAGCTGGTTTAATGAAATGGTAGGGAATGGTTGTGCACCAAACGTGGTAACTTATACGGCTCTCATACATGCTTACCTTAAAGCAAGGAAGGTGCGTGATGCAAATCAACTCTTTGAGATGATGTTGACTCAAGGTTGCATTCCTAATGCTGTTACTTATAGTGCCTTGATTGATGGCCACTGTAAAGCTGGAGAGACTGAAAAGGCTTGCCTGATTTATGCAAGAATGAGAGGTGATGTAAATGTCCCTGATGTTGATATGTATTTCAAAATTGCTGATCAGAGTTTGAAAGAGCCTAATGTTCATACTTATGGAGCTTTGGTGGATGGTTTATGTAAAGCCAACAAGGTCAAAGAGGCCGGTGAGTTATTGGATGCTATGTTCGTAGAAGGTTGTGAACCTAACCACATTGTTTATGACGCTCTTATAGATGGATTTTGCAAGTCTGGGAAGCTCGATGAAGCACAAAAGGTGTTTGCCAAAATGTCAGAGCACGGGTATAGTCCAAATGTGTACACGTACAGCTCCTTGATTGACAGGTTGTTCAAGGATAAAAGACTGGATCTTGTTTTAAAAGTCTTGTCTAAAATGTTAGAAGAATCTTGTTCACCAAATGTTGTTATCTATACAGAGATGGTCGATGGCCTTTGTAAGGTTGGAAAGACTGATGAAGCTTATAAGCTTATGTTAATGATGGAAGAAAAAGGATGTAATCCTAACGTTGTGACTTACACTGCAATGATAGATGGCCTTGGGAAAGCAGGCAGAATAGACAAATGTCTTGAGCTCTTTAAAACAATGAGCTCCAATGGTTGTGCTCCCAATTTTATCACCTACAAGGTTCTGATTAACCATTGCTGTGCACATGGTCTTTTGGATGAAGCTCATAAACTTTTGGATGAAATGAAACAAACATATTGGCCAAAGCATCTGGCAGGCTACCGTAAAGTTATTGAAGGTTACAACCGAGAGTTCATAGCCTCACTTGGGCTTTTAAGTGAGATAAGTGAGTGTGATTCGTTGCCAATCGCTCACATATACAGAGTTTTGGTTGATAATTTTGTTAAGGCTGGAAGACTGAACGTGGCTCTGGAGCTGCATGAAGAGATTTCTTCTTCTACCCCTTTTACATCTGCTAACAAGGATATGTATACTTTACTGATTGAGAACCTGTCACATGCAAATAAGGCTGATAAGGCACTGCAGATGTTTGCAGAAATGATAAGGCTGGGTGGTTATCCAGAGCTTAGTACATTTTTCCACCTTATCAAAGGGCTTATTAAAATTAATAGGTGGGATGAAGCTCTTCAACTATCGGATAGCATATGTCAGATGGATATCCAATGGCTTCTGCAAGAAGAGACGTATGACAGGAAGTAG

Protein Analysis

990

Amino Acids

111.06

Weight (kDa)

6.31

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 292 - 340 3.3e-12 PPR repeat family
PPR_3 PF13812 358 - 411 2.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 365 - 410 5.9e-09 PPR repeat family
PPR_long PF17177 375 - 524 7.3e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 449 - 487 1.4e-08 PPR repeat family
TPR_24 PF23276 453 - 569 7e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 461 - 519 6.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 469 - 502 1.8e-06 PPR repeat
PPR_2 PF13041 473 - 522 6.6e-16 PPR repeat family
PPR_long PF17177 497 - 608 2.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 504 - 537 1.7e-08 PPR repeat
PPR PF01535 511 - 541 8.8e-07 PPR repeat
PPR_2 PF13041 516 - 554 4.9e-09 PPR repeat family
PPR_3 PF13812 533 - 588 2.3e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 539 - 571 4.3e-08 PPR repeat
PPR_2 PF13041 543 - 592 5.7e-18 PPR repeat family
PPR PF01535 546 - 576 2.4e-06 PPR repeat
PPR_1 PF12854 575 - 606 3.2e-09 PPR repeat
PPR_1 PF12854 628 - 657 7.6e-09 PPR repeat
PPR_2 PF13041 629 - 678 8e-13 PPR repeat family
PPR_3 PF13812 661 - 709 4.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 661 - 693 1.2e-12 PPR repeat
PPR_2 PF13041 664 - 713 1.2e-15 PPR repeat family
PPR PF01535 668 - 696 4.6e-07 PPR repeat
PPR_3 PF13812 687 - 740 1.4e-08 Pentatricopeptide repeat domain
PPR_long PF17177 716 - 837 3.7e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 723 - 782 3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 731 - 762 1.4e-08 PPR repeat
PPR_2 PF13041 734 - 783 1.2e-16 PPR repeat family
PPR_3 PF13812 761 - 816 6.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 765 - 797 4.3e-11 PPR repeat
PPR_2 PF13041 769 - 817 8.3e-17 PPR repeat family
PPR PF01535 772 - 802 5.4e-09 PPR repeat
PPR_1 PF12854 800 - 833 2.8e-10 PPR repeat
PPR PF01535 807 - 834 7.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1152, 2268
AarI CACCTGC 1 cut(s) 1029
Acc36I ACCTGC 1 cut(s) 1029
AccI GTMKAC 3 cut(s) 442, 1530, 2741
AccII CGCG 2 cut(s) 219, 239
AciI CCGC 6 cut(s) 150, 161, 217, 237, 251, 610
AclI AACGTT 1 cut(s) 2310
AclWI GGATC 1 cut(s) 2160
AcoI YGGCCR 3 cut(s) 1354, 1765, 2510
AcsI RAATTY 2 cut(s) 1050, 1364
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 6 cut(s) 307, 668, 1429, 2105, 2111, 2842
AfiI CCNNNNNNNGG 2 cut(s) 2242, 2577
AflII CTTAAG 1 cut(s) 653
AflIII ACRYGT 1 cut(s) 2106
AhlI ACTAGT 1 cut(s) 1424
AjnI CCWGG 1 cut(s) 1189
AjuI GAANNNNNNNTTGG 6 cut(s) 64, 96, 356, 388, 617, 649
AloI GAACNNNNNNTCC 4 cut(s) 26, 58, 1955, 1987
Alw21I GWGCWC 7 cut(s) 266, 1627, 2085, 2379, 2396, 2410, 2455
Alw26I GTCTC 4 cut(s) 27, 185, 1778, 2945
Alw44I GTGCAC 2 cut(s) 1623, 2451
AlwI GGATC 1 cut(s) 2160
AoxI GGCC 9 cut(s) 671, 681, 1354, 1570, 1765, 1947, 2233, 2338, 2510
ApaLI GTGCAC 2 cut(s) 1623, 2451
ApeKI GCWGC 1 cut(s) 2686
ApoI RAATTY 2 cut(s) 1050, 1364
AseI ATTAAT 1 cut(s) 2877
Asp700I GAANNNNTTC 2 cut(s) 534, 2895
AspLEI GCGC 4 cut(s) 241, 739, 814, 963
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 6 cut(s) 566, 1298, 1835, 1964, 2190, 2413
AxyI CCTNAGG 1 cut(s) 341
BaeGI GKGCMC 3 cut(s) 876, 1627, 2455
BalI TGGCCA 3 cut(s) 1356, 1767, 2512
BanII GRGCYC 2 cut(s) 2379, 2396
BbsI GAAGAC 3 cut(s) 16, 207, 2671
Bbv12I GWGCWC 7 cut(s) 266, 1627, 2085, 2379, 2396, 2410, 2455
BbvI GCAGC 1 cut(s) 2673
BceAI ACGGC 3 cut(s) 260, 750, 1662
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 1 cut(s) 1191
BclI TGATCA 2 cut(s) 1065, 1867
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 4 cut(s) 27, 185, 1778, 2945
BcuI ACTAGT 1 cut(s) 1424
BfaI CTAG 5 cut(s) 644, 1086, 1092, 1425, 1547
BfmI CTRYAG 1 cut(s) 2792
BfrI CTTAAG 1 cut(s) 653
BfuAI ACCTGC 1 cut(s) 1029
BglI GCCNNNNNGGC 1 cut(s) 1191
BisI GCNGC 2 cut(s) 610, 2687
BlsI GCNGC 2 cut(s) 611, 2688
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 2 cut(s) 188, 1191
BmrFI CCNGG 2 cut(s) 188, 1191
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 5 cut(s) 301, 1281, 1678, 1954, 2527
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 2367
BpiI GAAGAC 3 cut(s) 16, 207, 2671
BpmI CTGGAG 4 cut(s) 121, 518, 1800, 2702
BpuEI CTTGAG 2 cut(s) 1704, 2393
BpuMI CCSGG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 2109
BsaBI GATNNNNATC 2 cut(s) 1692, 2927
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 186, 1357, 1467, 2341
BsaXI ACNNNNNCTCC 8 cut(s) 26, 56, 167, 197, 755, 785, 1896, 1926
Bsc4I CCNNNNNNNGG 2 cut(s) 2242, 2577
Bse118I RCCGGY 1 cut(s) 1949
Bse1I ACTGG 3 cut(s) 469, 501, 2154
Bse21I CCTNAGG 1 cut(s) 341
Bse3DI GCAATG 5 cut(s) 619, 1180, 1465, 2334, 2443
Bse8I GATNNNNATC 2 cut(s) 1692, 2927
BseBI CCWGG 1 cut(s) 1191
BseDI CCNNGG 4 cut(s) 186, 1357, 1467, 2341
BseJI GATNNNNATC 2 cut(s) 1692, 2927
BseLI CCNNNNNNNGG 2 cut(s) 2242, 2577
BseMI GCAATG 5 cut(s) 619, 1180, 1465, 2334, 2443
BseMII CTCAG 2 cut(s) 399, 1601
BseNI ACTGG 3 cut(s) 469, 501, 2154
BseRI GAGGAG 2 cut(s) 145, 172
BseSI GKGCMC 3 cut(s) 876, 1627, 2455
BseXI GCAGC 1 cut(s) 2673
BseYI CCCAGC 2 cut(s) 824, 2818
Bsh1236I CGCG 2 cut(s) 219, 239
BshFI GGCC 9 cut(s) 673, 683, 1356, 1572, 1767, 1949, 2235, 2340, 2512
BsiHKAI GWGCWC 7 cut(s) 266, 1627, 2085, 2379, 2396, 2410, 2455
BsiSI CCGG 3 cut(s) 188, 527, 1950
BslFI GGGAC 1 cut(s) 1820
BslI CCNNNNNNNGG 2 cut(s) 2242, 2577
BsmAI GTCTC 4 cut(s) 27, 185, 1778, 2945
BsmBI CGTCTC 1 cut(s) 2945
BsmFI GGGAC 1 cut(s) 1820
BsmI GAATGC 3 cut(s) 963, 975, 1728
BsnI GGCC 9 cut(s) 673, 683, 1356, 1572, 1767, 1949, 2235, 2340, 2512
Bso31I GGTCTC 1 cut(s) 185
Bsp1286I GDGCHC 8 cut(s) 266, 876, 1627, 2085, 2379, 2396, 2410, 2455
Bsp1407I TGTACA 1 cut(s) 2103
Bsp143I GATC 4 cut(s) 1065, 1127, 1867, 2152
Bsp19I CCATGG 1 cut(s) 1357
BspACI CCGC 6 cut(s) 150, 161, 217, 237, 251, 610
BspANI GGCC 9 cut(s) 673, 683, 1356, 1572, 1767, 1949, 2235, 2340, 2512
BspCNI CTCAG 2 cut(s) 400, 1600
BspFNI CGCG 2 cut(s) 219, 239
BspMAI CTGCAG 1 cut(s) 2796
BspMI ACCTGC 1 cut(s) 1029
BspPI GGATC 1 cut(s) 2160
BspQI GCTCTTC 2 cut(s) 633, 2901
BspTI CTTAAG 1 cut(s) 653
BspTNI GGTCTC 1 cut(s) 185
BsrDI GCAATG 5 cut(s) 619, 1180, 1465, 2334, 2443
BsrFI RCCGGY 1 cut(s) 1949
BsrGI TGTACA 1 cut(s) 2103
BsrI ACTGG 3 cut(s) 469, 501, 2154
BssAI RCCGGY 1 cut(s) 1949
BssECI CCNNGG 4 cut(s) 186, 1357, 1467, 2341
BssMI GATC 4 cut(s) 1065, 1127, 1867, 2152
BssNAI GTATAC 1 cut(s) 2742
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 3 cut(s) 1357, 1467, 2341
Bst1107I GTATAC 1 cut(s) 2742
Bst2UI CCWGG 1 cut(s) 1191
Bst4CI ACNGT 4 cut(s) 883, 1514, 1772, 2534
Bst6I CTCTTC 7 cut(s) 58, 395, 633, 858, 2688, 2901, 2943
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 653
BstAPI GCANNNNNTGC 1 cut(s) 606
BstAUI TGTACA 1 cut(s) 2103
BstBAI YACGTR 1 cut(s) 2109
BstC8I GCNNGC 7 cut(s) 846, 936, 1187, 1570, 1798, 2353, 2527
BstDEI CTNAG 7 cut(s) 341, 408, 1055, 1252, 1563, 1587, 2837
BstDSI CCRYGG 1 cut(s) 1357
BstENI CCTNNNNNAGG 1 cut(s) 2240
BstFNI CGCG 2 cut(s) 219, 239
BstHHI GCGC 4 cut(s) 241, 739, 814, 963
BstKTI GATC 4 cut(s) 1068, 1130, 1870, 2155
BstMAI GTCTC 4 cut(s) 27, 185, 1778, 2945
BstMBI GATC 4 cut(s) 1065, 1127, 1867, 2152
BstMWI GCNNNNNNNGC 8 cut(s) 606, 649, 960, 1191, 1277, 1455, 2777, 2786
BstNI CCWGG 1 cut(s) 1191
BstNSI RCATGY 2 cut(s) 1661, 2771
BstPAI GACNNNNGTC 1 cut(s) 2367
BstSCI CCNGG 2 cut(s) 186, 1189
BstSFI CTRYAG 1 cut(s) 2792
BstSLI GKGCMC 3 cut(s) 876, 1627, 2455
BstUI CGCG 2 cut(s) 219, 239
BstV1I GCAGC 1 cut(s) 2673
BstV2I GAAGAC 3 cut(s) 16, 207, 2671
BstX2I RGATCY 1 cut(s) 2152
BstYI RGATCY 1 cut(s) 2152
BstZ17I GTATAC 1 cut(s) 2742
Bsu36I CCTNAGG 1 cut(s) 341
BsuRI GGCC 9 cut(s) 673, 683, 1356, 1572, 1767, 1949, 2235, 2340, 2512
BtgI CCRYGG 1 cut(s) 1357
BtsI GCAGTG 2 cut(s) 2322, 2789
BtsIMutI CAGTG 4 cut(s) 462, 1768, 2322, 2789
BveI ACCTGC 1 cut(s) 1029
Cac8I GCNNGC 7 cut(s) 846, 936, 1187, 1570, 1798, 2353, 2527
CfoI GCGC 4 cut(s) 241, 739, 814, 963
Cfr10I RCCGGY 1 cut(s) 1949
CseI GACGC 2 cut(s) 281, 2018
Csp6I GTAC 6 cut(s) 306, 667, 1428, 2104, 2110, 2841
CviQI GTAC 6 cut(s) 306, 667, 1428, 2104, 2110, 2841
DdeI CTNAG 7 cut(s) 341, 408, 1055, 1252, 1563, 1587, 2837
DpnI GATC 4 cut(s) 1067, 1129, 1869, 2154
DpnII GATC 4 cut(s) 1065, 1127, 1867, 2152
DraI TTTAAA 2 cut(s) 2163, 2383
EaeI YGGCCR 3 cut(s) 1354, 1765, 2510
Eam1104I CTCTTC 7 cut(s) 58, 395, 633, 858, 2688, 2901, 2943
EarI CTCTTC 7 cut(s) 58, 395, 633, 858, 2688, 2901, 2943
EciI GGCGGA 3 cut(s) 139, 150, 240
Ecl136II GAGCTC 2 cut(s) 2377, 2394
Eco130I CCWWGG 3 cut(s) 1357, 1467, 2341
Eco24I GRGCYC 2 cut(s) 2379, 2396
Eco31I GGTCTC 1 cut(s) 185
Eco32I GATATC 2 cut(s) 903, 2929
Eco53kI GAGCTC 2 cut(s) 2377, 2394
Eco81I CCTNAGG 1 cut(s) 341
EcoICRI GAGCTC 2 cut(s) 2377, 2394
EcoNI CCTNNNNNAGG 1 cut(s) 2240
EcoRI GAATTC 1 cut(s) 1050
EcoRII CCWGG 1 cut(s) 1189
EcoRV GATATC 2 cut(s) 903, 2929
EcoT14I CCWWGG 3 cut(s) 1357, 1467, 2341
EcoT22I ATGCAT 1 cut(s) 316
EcoT38I GRGCYC 2 cut(s) 2379, 2396
ErhI CCWWGG 3 cut(s) 1357, 1467, 2341
Esp3I CGTCTC 1 cut(s) 2945
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 1 cut(s) 1820
FauNDI CATATG 1 cut(s) 2915
FbaI TGATCA 2 cut(s) 1065, 1867
FblI GTMKAC 3 cut(s) 442, 1530, 2741
Fnu4HI GCNGC 2 cut(s) 610, 2687
FriOI GRGCYC 2 cut(s) 2379, 2396
Fsp4HI GCNGC 2 cut(s) 610, 2687
FspBI CTAG 5 cut(s) 644, 1086, 1092, 1425, 1547
GlaI GCGC 4 cut(s) 240, 738, 813, 962
GluI GCNGC 2 cut(s) 610, 2687
GsaI CCCAGC 2 cut(s) 828, 2822
GsuI CTGGAG 4 cut(s) 121, 518, 1800, 2702
HaeIII GGCC 9 cut(s) 673, 683, 1356, 1572, 1767, 1949, 2235, 2340, 2512
HapII CCGG 3 cut(s) 188, 527, 1950
HgaI GACGC 2 cut(s) 281, 2018
HhaI GCGC 4 cut(s) 241, 739, 814, 963
Hin1I GRCGYC 1 cut(s) 273
Hin6I GCGC 4 cut(s) 239, 737, 812, 961
HinP1I GCGC 4 cut(s) 239, 737, 812, 961
HincII GTYRAC 1 cut(s) 1716
HindII GTYRAC 1 cut(s) 1716
HindIII AAGCTT 5 cut(s) 918, 1278, 1374, 2262, 2270
HpaII CCGG 3 cut(s) 188, 527, 1950
HphI GGTGA 6 cut(s) 566, 1298, 1835, 1964, 2190, 2413
Hpy188I TCNGA 8 cut(s) 232, 1132, 1590, 1872, 2080, 2436, 2908, 2922
Hpy188III TCNNGA 9 cut(s) 31, 138, 173, 1213, 1418, 1523, 2372, 2681, 2831
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 6 cut(s) 58, 100, 1067, 1672, 1973, 2540
HpyCH4III ACNGT 4 cut(s) 883, 1514, 1772, 2534
HpyCH4IV ACGT 7 cut(s) 791, 992, 1632, 2108, 2310, 2673, 2954
HpyF10VI GCNNNNNNNGC 8 cut(s) 606, 649, 960, 1191, 1277, 1455, 2777, 2786
HpyF3I CTNAG 7 cut(s) 341, 408, 1055, 1252, 1563, 1587, 2837
HpySE526I ACGT 7 cut(s) 791, 992, 1632, 2108, 2310, 2673, 2954
Hsp92I GRCGYC 1 cut(s) 273
HspAI GCGC 4 cut(s) 239, 737, 812, 961
Ksp22I TGATCA 2 cut(s) 1065, 1867
Kzo9I GATC 4 cut(s) 1065, 1127, 1867, 2152
LguI GCTCTTC 2 cut(s) 633, 2901
LmnI GCTCC 6 cut(s) 499, 1904, 2120, 2399, 2413, 2683
Lsp1109I GCAGC 1 cut(s) 2673
LweI GCATC 5 cut(s) 301, 1281, 1678, 1954, 2527
MaeI CTAG 5 cut(s) 644, 1086, 1092, 1425, 1547
MaeII ACGT 7 cut(s) 791, 992, 1632, 2108, 2310, 2673, 2954
MaeIII GTNAC 7 cut(s) 458, 492, 1636, 1741, 2314, 2549, 2763
MalI GATC 4 cut(s) 1067, 1129, 1869, 2154
MboI GATC 4 cut(s) 1065, 1127, 1867, 2152
MflI RGATCY 1 cut(s) 2152
MhlI GDGCHC 8 cut(s) 266, 876, 1627, 2085, 2379, 2396, 2410, 2455
MlsI TGGCCA 3 cut(s) 1356, 1767, 2512
MluNI TGGCCA 3 cut(s) 1356, 1767, 2512
MlyI GAGTC 4 cut(s) 215, 400, 685, 1711
MmeI TCCRAC 2 cut(s) 95, 2227
Mox20I TGGCCA 3 cut(s) 1356, 1767, 2512
Mph1103I ATGCAT 1 cut(s) 316
MroXI GAANNNNTTC 2 cut(s) 534, 2895
MscI TGGCCA 3 cut(s) 1356, 1767, 2512
MslI CAYNNNNRTG 3 cut(s) 1899, 2327, 2448
Msp20I TGGCCA 3 cut(s) 1356, 1767, 2512
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 3 cut(s) 188, 527, 1950
MspR9I CCNGG 2 cut(s) 188, 1191
Mva1269I GAATGC 3 cut(s) 963, 975, 1728
MvaI CCWGG 1 cut(s) 1191
MvnI CGCG 2 cut(s) 219, 239
MwoI GCNNNNNNNGC 8 cut(s) 606, 649, 960, 1191, 1277, 1455, 2777, 2786
NciI CCSGG 1 cut(s) 188
NcoI CCATGG 1 cut(s) 1357
NdeI CATATG 1 cut(s) 2915
NdeII GATC 4 cut(s) 1065, 1127, 1867, 2152
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 3 cut(s) 458, 2314, 2763
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 2 cut(s) 1661, 2771
PaqCI CACCTGC 1 cut(s) 1029
PciSI GCTCTTC 2 cut(s) 633, 2901
PctI GAATGC 3 cut(s) 963, 975, 1728
PdmI GAANNNNTTC 2 cut(s) 534, 2895
PfeI GAWTC 6 cut(s) 141, 233, 595, 745, 2189, 2606
PkrI GCNGC 2 cut(s) 611, 2688
PleI GAGTC 4 cut(s) 215, 399, 684, 1711
PpsI GAGTC 4 cut(s) 215, 399, 684, 1711
Ppu21I YACGTR 1 cut(s) 2109
PshAI GACNNNNGTC 1 cut(s) 2367
PshBI ATTAAT 1 cut(s) 2877
PsiI TTATAA 2 cut(s) 1152, 2268
Psp124BI GAGCTC 2 cut(s) 2379, 2396
Psp1406I AACGTT 1 cut(s) 2310
Psp6I CCWGG 1 cut(s) 1189
PspFI CCCAGC 2 cut(s) 824, 2818
PspGI CCWGG 1 cut(s) 1189
PstI CTGCAG 1 cut(s) 2796
PsuI RGATCY 1 cut(s) 2152
RsaI GTAC 6 cut(s) 307, 668, 1429, 2105, 2111, 2842
RsaNI GTAC 6 cut(s) 306, 667, 1428, 2104, 2110, 2841
RseI CAYNNNNRTG 3 cut(s) 1899, 2327, 2448
SacI GAGCTC 2 cut(s) 2379, 2396
SapI GCTCTTC 2 cut(s) 633, 2901
SatI GCNGC 2 cut(s) 610, 2687
Sau3AI GATC 4 cut(s) 1065, 1127, 1867, 2152
ScaI AGTACT 1 cut(s) 307
SchI GAGTC 4 cut(s) 215, 400, 685, 1711
ScrFI CCNGG 2 cut(s) 188, 1191
SduI GDGCHC 8 cut(s) 266, 876, 1627, 2085, 2379, 2396, 2410, 2455
SfaNI GCATC 5 cut(s) 301, 1281, 1678, 1954, 2527
SfcI CTRYAG 1 cut(s) 2792
SmiMI CAYNNNNRTG 3 cut(s) 1899, 2327, 2448
SmlI CTYRAG 3 cut(s) 653, 1719, 2372
SmoI CTYRAG 3 cut(s) 653, 1719, 2372
SpeI ACTAGT 1 cut(s) 1424
SsiI CCGC 6 cut(s) 150, 161, 217, 237, 251, 610
SspMI CTAG 5 cut(s) 644, 1086, 1092, 1425, 1547
SstI GAGCTC 2 cut(s) 2379, 2396
StyD4I CCNGG 2 cut(s) 186, 1189
StyI CCWWGG 3 cut(s) 1357, 1467, 2341
TaaI ACNGT 4 cut(s) 883, 1514, 1772, 2534
TaiI ACGT 7 cut(s) 794, 995, 1635, 2111, 2313, 2676, 2957
TaqI TCGA 4 cut(s) 174, 1551, 2046, 2229
TaqII GACCGA 1 cut(s) 794
TatI WGTACW 4 cut(s) 305, 1427, 2103, 2840
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 6 cut(s) 141, 233, 595, 745, 2189, 2606
TscAI CASTG 4 cut(s) 469, 1775, 2329, 2796
TseFI GTSAC 3 cut(s) 458, 2314, 2763
TseI GCWGC 1 cut(s) 2686
Tsp45I GTSAC 3 cut(s) 458, 2314, 2763
TspRI CASTG 4 cut(s) 469, 1775, 2329, 2796
Vha464I CTTAAG 1 cut(s) 653
VneI GTGCAC 2 cut(s) 1623, 2451
VspI ATTAAT 1 cut(s) 2877
XagI CCTNNNNNAGG 1 cut(s) 2240
XapI RAATTY 2 cut(s) 1050, 1364
XceI RCATGY 2 cut(s) 1661, 2771
XcmI CCANNNNNNNNNTGG 1 cut(s) 1775
XmiI GTMKAC 3 cut(s) 442, 1530, 2741
XmnI GAANNNNTTC 2 cut(s) 534, 2895
XspI CTAG 5 cut(s) 644, 1086, 1092, 1425, 1547
ZrmI AGTACT 1 cut(s) 307
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.