Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0003234

Physical Location & Seq

Genomic Coordinates Reverse (-)

26287298 .. 26293722

Gene Structure 6425 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG147200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3015 bp

ATGAGCAGAAGACACTTCAGCTCTCTACTCTTCAACTCCATCTCTTCTTCTCCCCTTTTCTCCAGAAACCCATCCCAATCCTTCGTTTCTTCACTCAAATCATACCCCCAAAATCTCAATCTCTGCCGCCAATGTCTCTTCTCCAGATTCCTCTGCACCTCTCCGCCGGACAATCTCGACGGCCTCCTCGATCCCCAGGACTTCTCTACGCCGGAGACCCACTCTCTTTCCGCACAAGACTTCGCCTTTCTGCGCGACTCGCCGAAACTTGACCCCGATAAGTGTTCTAACGACGCCGTTTTGATTTCTAATGCCATTAGGAATAGTTCCGATTCATTTGGGGATGAAACCCACAAGTTTCTTAGGAGGTTTAGGGATAATCTGAATGAAACTTTGGTGGTGCAGGTGTTGAGTCTTGTAAAGAGTCCTGAGTTGGGTGTCAAGTTTTTCATATGGGCGGGTAGGCAAATTGGGTATAGTCATACTGCTTCCGTATATAATGCATTGTTGGAGTTACTGGAGTGTGGTAGTAGTAGTGACAGAGTACCGGAACATTTCTTGCGAGAAATTAAGGGTGAGGATAGAGAAGTGCTTAGGAAGTTGCTTAATGTGTTGATTCGCAAGTGCTGCCGAAATGGGTTGTGGAATGTAGCTTTGGAAGAGCTTGGGAGGCTTAAGGATTTCGGGTACTGGCCGAGTCAAGCAACTTACAATGCTTTGGTTCAGGTGTTTTTGAGAGCTGATAGGCTGGACACGGCGCACTTGGTTCATGGGGAGATGTTGGAGTTGGGGTTTAAGATGGATGAGTATACATTAGGTTGTTTCGGTCATGCCCTGTGTAAAGCTGGGAGGTGGAAGGAGGGACTTGCATTGATTGAGAAGGAAGAGTTTGTGCCCAATACTGTTCTTTATACCAAGATGATATCTGGGTTGTGTGAGGCTTCACTTTTTGAGGAAGCTATGGATTTGTTGAACAGAATGCGCTGTAATTCTTGCATTCCTAATGTTGTAACCTATAGGATTTTGCTTTGTGGATGTTTGAAGAAAAAACAGCTTGGCAGGTGCAAGAGAATTCTTAGTATGATGATCACGGAAGGTTGTTATCCTAGCCCTGGGATATTTAATTCTCTTGTTCATGCTTATTGCAGATCAGGAGATTACTCTTATGCTTATAAGTTGCTAAAGAAAATGGTCAGATGTGGGTGCCGGCCAGGCTATGTGGTTTACAATATCTTGATTGGTGGTATCTGTGGACACGAAGAATTACCTACCTTAGATATGCTAGACATGGCTGAAAAAGCTTATGGTGAGATGCTTAATGCAGGGGTTGTTTTAAATAAGGTAAATGTAAGCAATTTTGTGCGATGTCTTTGTGGCCATGGGAAATTTGACAAAGCCTACAAGGTCATCAATGAAATGATGAGCAAGGGTTTTGTTCCTGACACTAGTACATACTCTAAAGTTATTGGCTTTTTGTGCAATGCCTCCAAGGTAGAACAAGCATTATTGTTGTTTGAAGAAATGAAGCAAAACGGTGTTGTTCCTGATGTCTACACATATACCATCTTAATTGATAGTTTTTCTAAGGCTGGCCTTATTGAACAGGCTCGGAACTGGTTTAATGAAATGGTAGGAAATGGTTGTGCACCAAACGTGGTAACTTATACTGCTCTTATACATGCATACCTTAAAGCAAGGAAGGTGCCTGATGCAAATCAACTCTTTGAGATGATGTTGACTCAAGGTTGCATTCCTAATGCTATTACTTATAGTGCTTTGATCGATGGCCATTGTAAAGCTGGAGAGACTGAAAAGGCTTGCCTGATTTATGCAAGAATGAGAGGTAATGTGGAAGTCCCTGATGTTGATATGTATTTCAAAATTGATGATCAGAGTTTGAAAGAACCTAATGTTCATACTTATGGAGCTTTGGTGGATGGTTTATGTAAAGCCAACAAGGTCAGAGAGGCCGGTGACTTATTGGATGCTATGTTGGTAGAAGGTTGTGAACCTAACCACATTGTTTATGATGCTCTTATAGATGGATTTTGCAAGTCTGGGAAGCTTGATGAAGCACAAAAGGTGTTTGCTAAAATGTCAGAGCATGGGTATAGTCCAAATGTGTATACGTACAGCTCTTTGATTGACAGGTTGTTCAAGGATAAAAGACTGGATCTTGTTTTAAAAGTCCTGTCTAAAATGTTAGAAAACTCATGTTCACCAAATGTTGTTATCTATACAGAGATGGTCGATGGCCTTTGTAAGGTTGGAAAGACAGATGAAGCTTATAAGCTTATGCTAATGATGGAAGAAAAAGGATGTAATCCAAACGTTGTGACTTACACTGCAATGATAGATGGCCTTGGGAAAGCAGGCAAAATCGACAAATGTCTTGAGCTCTTTAAAGCAATGAGCTCCAAGGGTTGTGCTCCCAATTTTATCACCTACAAGGTTTTGATAAACCATTGCTGTGCAATTGGTCTTTTGGATGAAGCTCATATACTTTTGGATGAAATGAAACAAACATATTGGCCAAAGCATTTGGCAGGCTACCGTAAAGTTATTGAAGGTTACAACCGAGAATTCATAGCCTCACTTGGGCTTTTAAATGAGATAAGTGAGTGCGACTCATTGCCAATCGTTCACATATACAAAGTTTTGGTTGATAATTTTGTAAAGGCTGGAAGACTGGAAGTGGCTCTGGAGCTGCATGAAGAGATTTCTTCATCTACCCCTTTTACATCTGTGAACAAGGATATGTATACTTTACTGATCGAGAACCTATCACATGCAAATAAGGCTGATAAGGCACTGCAGATGTTTGCAGAAATGATAAGGCTCGGTGGTTATCCAGAGATGAGTACATTTTTCCACCTCATCAAAGGGCTTATTAAAATTAATAGGTGGGATGAAGCTCTTCAACTATCGGATAGCATATGCCAGATGGTTTGTCTTTCTGACTCCCTTCTCCTTCTTACCACAGTACATTATACTAAAATCCATTCTTTTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

1004

Amino Acids

112.88

Weight (kDa)

6.51

Isoelectric Point (pI)

36.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	232 - 281	3.9e-06	PPR repeat family
PPR_1	PF12854	297 - 328	1.2e-06	PPR repeat
PPR_2	PF13041	299 - 348	1.6e-14	PPR repeat family
PPR_1	PF12854	366 - 398	1.7e-06	PPR repeat
PPR_3	PF13812	370 - 416	2.6e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	373 - 417	4.1e-10	PPR repeat family
PPR	PF01535	373 - 402	3.8e-07	PPR repeat
PPR_long	PF17177	382 - 531	8.4e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	453 - 494	7.4e-09	PPR repeat family
PPR_3	PF13812	468 - 527	4.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	484 - 529	4.4e-14	PPR repeat family
PPR_long	PF17177	498 - 616	9.4e-11	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	511 - 544	6e-09	PPR repeat
PPR	PF01535	518 - 548	2.4e-07	PPR repeat
PPR_2	PF13041	523 - 562	2.4e-10	PPR repeat family
PPR_3	PF13812	540 - 595	1.3e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	546 - 578	4.6e-07	PPR repeat
PPR_2	PF13041	550 - 599	5.1e-17	PPR repeat family
PPR	PF01535	553 - 583	8.2e-06	PPR repeat
PPR_1	PF12854	582 - 613	7.6e-09	PPR repeat
PPR_1	PF12854	635 - 664	4.4e-08	PPR repeat
PPR_2	PF13041	636 - 685	4.6e-13	PPR repeat family
PPR_3	PF13812	660 - 716	3.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	668 - 700	1.2e-12	PPR repeat
PPR_2	PF13041	671 - 720	1.3e-15	PPR repeat family
PPR	PF01535	675 - 703	4.7e-07	PPR repeat
PPR_3	PF13812	694 - 747	1.2e-08	Pentatricopeptide repeat domain
PPR_long	PF17177	723 - 844	1.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	730 - 789	3.3e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	738 - 769	1.4e-08	PPR repeat
PPR_2	PF13041	741 - 790	1.2e-16	PPR repeat family
PPR_3	PF13812	768 - 823	1.1e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	772 - 804	1.6e-10	PPR repeat
PPR_2	PF13041	776 - 824	6e-17	PPR repeat family
PPR	PF01535	779 - 809	3.1e-09	PPR repeat
PPR_1	PF12854	807 - 840	8.4e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003234)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710 AT1G06710
fragaria_vesca	FvH4_4g11500
malus_domestica	MD04G1034700.v1.1
prunus_persica	Prupe.1G186300_v2.0.a1
pyrus_communis	pycom04g02410 pycom04g02880
rosa_chinensis	RchiOBHm_Chr4g0408411
rosa_laevigata	RLG00000008653
rosa_multiflora	Rmu_ssc0000173.1_g000003
rosa_roxburghii	Rroxscaffold_5G00352870
rosa_rugosa	Rorug04G0085100 Rorug04G0085200 Rorug04G0085300 Rorug04G0085400
rosa_samantha	Rh4AG150300 Rh4BG147200 Rh4CG157600 Rh4DG143200
rosa_wichuraiana	Rw4G012270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1173, 2289
AarI	CACCTGC	2 cut(s)	394, 1050
Acc36I	ACCTGC	2 cut(s)	394, 1050
AccB1I	GGYRCC	2 cut(s)	1203, 1702
AccI	GTMKAC	4 cut(s)	809, 1551, 2126, 2762
AccII	CGCG	1 cut(s)	255
AciI	CCGC	4 cut(s)	127, 164, 231, 458
AclI	AACGTT	1 cut(s)	2331
AclWI	GGATC	2 cut(s)	185, 2181
AcoI	YGGCCR	5 cut(s)	692, 1208, 1375, 1786, 2531
AcsI	RAATTY	3 cut(s)	1071, 1385, 2582
AcyI	GRCGYC	1 cut(s)	294
AfaI	GTAC	6 cut(s)	546, 689, 1450, 2132, 2863, 2985
AfiI	CCNNNNNNNGG	4 cut(s)	434, 1112, 2263, 2598
AflII	CTTAAG	1 cut(s)	674
AhlI	ACTAGT	1 cut(s)	1445
AjnI	CCWGG	3 cut(s)	195, 1111, 1210
AjuI	GAANNNNNNNTTGG	4 cut(s)	377, 409, 638, 670
Alw21I	GWGCWC	4 cut(s)	1648, 2400, 2417, 2431
Alw26I	GTCTC	3 cut(s)	140, 209, 1799
Alw44I	GTGCAC	1 cut(s)	1644
AlwI	GGATC	2 cut(s)	185, 2181
ApaLI	GTGCAC	1 cut(s)	1644
ApeKI	GCWGC	2 cut(s)	627, 2707
ApoI	RAATTY	3 cut(s)	1071, 1385, 2582
AseI	ATTAAT	1 cut(s)	2898
Asp700I	GAANNNNTTC	1 cut(s)	2916
AspLEI	GCGC	3 cut(s)	255, 760, 984
AsuHPI	GGTGA	5 cut(s)	587, 1319, 1985, 2211, 2434
BaeGI	GKGCMC	2 cut(s)	897, 1648
BalI	TGGCCA	3 cut(s)	1377, 1788, 2533
BanI	GGYRCC	2 cut(s)	1203, 1702
BanII	GRGCYC	2 cut(s)	2400, 2417
BarI	GAAGNNNNNNTAC	2 cut(s)	2484, 2516
BbsI	GAAGAC	2 cut(s)	16, 2692
Bbv12I	GWGCWC	4 cut(s)	1648, 2400, 2417, 2431
BbvI	GCAGC	2 cut(s)	614, 2694
BceAI	ACGGC	3 cut(s)	196, 281, 771
BciT130I	CCWGG	3 cut(s)	197, 1113, 1212
BclI	TGATCA	2 cut(s)	1086, 1888
BcoDI	GTCTC	3 cut(s)	140, 209, 1799
BcuI	ACTAGT	1 cut(s)	1445
BfaI	CTAG	3 cut(s)	1107, 1283, 1446
BfmI	CTRYAG	2 cut(s)	1015, 2813
BfrI	CTTAAG	1 cut(s)	674
BfuAI	ACCTGC	2 cut(s)	394, 1050
BglI	GCCNNNNNGGC	1 cut(s)	1212
BisI	GCNGC	3 cut(s)	127, 628, 2708
BlsI	GCNGC	3 cut(s)	128, 629, 2709
Bme1390I	CCNGG	3 cut(s)	197, 1113, 1212
BmiI	GGNNCC	2 cut(s)	1205, 1704
BmrFI	CCNGG	3 cut(s)	197, 1113, 1212
BmsI	GCATC	4 cut(s)	1302, 1699, 1975, 2020
BoxI	GACNNNNGTC	1 cut(s)	2388
BpiI	GAAGAC	2 cut(s)	16, 2692
BplI	GAGNNNNNCTC	4 cut(s)	206, 238, 2612, 2644
BpmI	CTGGAG	5 cut(s)	46, 127, 539, 1821, 2723
Bpu10I	CCTNAGC	1 cut(s)	593
BpuEI	CTTGAG	2 cut(s)	1725, 2414
Bsa29I	ATCGAT	1 cut(s)	1782
BsaAI	YACGTR	1 cut(s)	2130
BsaBI	GATNNNNATC	1 cut(s)	1713
BsaHI	GRCGYC	1 cut(s)	294
BsaI	GGTCTC	1 cut(s)	209
BsaJI	CCNNGG	7 cut(s)	195, 1111, 1112, 1378, 1488, 2362, 2418
BsaWI	WCCGGW	1 cut(s)	547
BsaXI	ACNNNNNCTCC	4 cut(s)	776, 806, 1917, 1947
Bsc4I	CCNNNNNNNGG	4 cut(s)	434, 1112, 2263, 2598
Bse118I	RCCGGY	2 cut(s)	1206, 1970
Bse1I	ACTGG	5 cut(s)	522, 695, 1619, 2175, 2694
Bse3DI	GCAATG	5 cut(s)	1486, 2355, 2415, 2464, 2630
Bse8I	GATNNNNATC	1 cut(s)	1713
BseBI	CCWGG	3 cut(s)	197, 1113, 1212
BseCI	ATCGAT	1 cut(s)	1782
BseDI	CCNNGG	7 cut(s)	195, 1111, 1112, 1378, 1488, 2362, 2418
BseJI	GATNNNNATC	1 cut(s)	1713
BseLI	CCNNNNNNNGG	4 cut(s)	434, 1112, 2263, 2598
BseMI	GCAATG	5 cut(s)	1486, 2355, 2415, 2464, 2630
BseMII	CTCAG	1 cut(s)	420
BseNI	ACTGG	5 cut(s)	522, 695, 1619, 2175, 2694
BseRI	GAGGAG	1 cut(s)	176
BseSI	GKGCMC	2 cut(s)	897, 1648
BseXI	GCAGC	2 cut(s)	614, 2694
BseYI	CCCAGC	1 cut(s)	845
BsgI	GTGCAG	2 cut(s)	139, 422
Bsh1236I	CGCG	1 cut(s)	255
BshNI	GGYRCC	2 cut(s)	1203, 1702
BshVI	ATCGAT	1 cut(s)	1782
BsiHKAI	GWGCWC	4 cut(s)	1648, 2400, 2417, 2431
BsiSI	CCGG	5 cut(s)	167, 212, 548, 1207, 1971
BslFI	GGGAC	2 cut(s)	876, 1841
BslI	CCNNNNNNNGG	4 cut(s)	434, 1112, 2263, 2598
BsmAI	GTCTC	3 cut(s)	140, 209, 1799
BsmFI	GGGAC	2 cut(s)	876, 1841
BsmI	GAATGC	3 cut(s)	984, 996, 1749
Bso31I	GGTCTC	1 cut(s)	209
Bsp1286I	GDGCHC	5 cut(s)	897, 1648, 2400, 2417, 2431
Bsp143I	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
Bsp19I	CCATGG	1 cut(s)	1378
BspACI	CCGC	4 cut(s)	127, 164, 231, 458
BspCNI	CTCAG	1 cut(s)	421
BspDI	ATCGAT	1 cut(s)	1782
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	2 cut(s)	1205, 1704
BspMAI	CTGCAG	1 cut(s)	2817
BspMI	ACCTGC	2 cut(s)	394, 1050
BspPI	GGATC	2 cut(s)	185, 2181
BspQI	GCTCTTC	2 cut(s)	654, 2922
BspT107I	GGYRCC	2 cut(s)	1203, 1702
BspTI	CTTAAG	1 cut(s)	674
BspTNI	GGTCTC	1 cut(s)	209
BsrDI	GCAATG	5 cut(s)	1486, 2355, 2415, 2464, 2630
BsrFI	RCCGGY	2 cut(s)	1206, 1970
BsrI	ACTGG	5 cut(s)	522, 695, 1619, 2175, 2694
BssAI	RCCGGY	2 cut(s)	1206, 1970
BssECI	CCNNGG	7 cut(s)	195, 1111, 1112, 1378, 1488, 2362, 2418
BssMI	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
BssNAI	GTATAC	3 cut(s)	810, 2127, 2763
BssNI	GRCGYC	1 cut(s)	294
BssT1I	CCWWGG	4 cut(s)	1378, 1488, 2362, 2418
Bst1107I	GTATAC	3 cut(s)	810, 2127, 2763
Bst2UI	CCWGG	3 cut(s)	197, 1113, 1212
Bst4CI	ACNGT	4 cut(s)	904, 1535, 2555, 2983
Bst6I	CTCTTC	7 cut(s)	35, 49, 143, 654, 879, 2709, 2922
BstACI	GRCGYC	1 cut(s)	294
BstAFI	CTTAAG	1 cut(s)	674
BstAPI	GCANNNNNTGC	1 cut(s)	627
BstBAI	YACGTR	1 cut(s)	2130
BstC8I	GCNNGC	5 cut(s)	1208, 1591, 1819, 2374, 2548
BstDEI	CTNAG	6 cut(s)	362, 429, 593, 1076, 1273, 1584
BstDSI	CCRYGG	1 cut(s)	1378
BstENI	CCTNNNNNAGG	1 cut(s)	2261
BstFNI	CGCG	1 cut(s)	255
BstHHI	GCGC	3 cut(s)	255, 760, 984
BstKTI	GATC	7 cut(s)	193, 1089, 1151, 1782, 1891, 2176, 2775
BstMAI	GTCTC	3 cut(s)	140, 209, 1799
BstMBI	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
BstMWI	GCNNNNNNNGC	8 cut(s)	259, 627, 670, 1212, 1298, 1476, 2798, 2807
BstNI	CCWGG	3 cut(s)	197, 1113, 1212
BstNSI	RCATGY	2 cut(s)	1682, 2792
BstPAI	GACNNNNGTC	1 cut(s)	2388
BstSCI	CCNGG	3 cut(s)	195, 1111, 1210
BstSFI	CTRYAG	2 cut(s)	1015, 2813
BstSLI	GKGCMC	2 cut(s)	897, 1648
BstSNI	TACGTA	1 cut(s)	2130
BstUI	CGCG	1 cut(s)	255
BstV1I	GCAGC	2 cut(s)	614, 2694
BstV2I	GAAGAC	2 cut(s)	16, 2692
BstX2I	RGATCY	1 cut(s)	2173
BstYI	RGATCY	1 cut(s)	2173
BstZ17I	GTATAC	3 cut(s)	810, 2127, 2763
Bsu15I	ATCGAT	1 cut(s)	1782
BsuTUI	ATCGAT	1 cut(s)	1782
BtgI	CCRYGG	1 cut(s)	1378
BtgZI	GCGATG	1 cut(s)	1378
BtsI	GCAGTG	2 cut(s)	2343, 2810
BtsIMutI	CAGTG	2 cut(s)	2343, 2810
BveI	ACCTGC	2 cut(s)	394, 1050
Cac8I	GCNNGC	5 cut(s)	1208, 1591, 1819, 2374, 2548
CfoI	GCGC	3 cut(s)	255, 760, 984
Cfr10I	RCCGGY	2 cut(s)	1206, 1970
ClaI	ATCGAT	1 cut(s)	1782
CseI	GACGC	1 cut(s)	302
Csp6I	GTAC	6 cut(s)	545, 688, 1449, 2131, 2862, 2984
CviQI	GTAC	6 cut(s)	545, 688, 1449, 2131, 2862, 2984
DdeI	CTNAG	6 cut(s)	362, 429, 593, 1076, 1273, 1584
DpnI	GATC	7 cut(s)	192, 1088, 1150, 1781, 1890, 2175, 2774
DpnII	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
DraI	TTTAAA	4 cut(s)	1335, 2184, 2404, 2607
EaeI	YGGCCR	5 cut(s)	692, 1208, 1375, 1786, 2531
Eam1104I	CTCTTC	7 cut(s)	35, 49, 143, 654, 879, 2709, 2922
EarI	CTCTTC	7 cut(s)	35, 49, 143, 654, 879, 2709, 2922
EciI	GGCGGA	1 cut(s)	153
Ecl136II	GAGCTC	2 cut(s)	2398, 2415
Eco105I	TACGTA	1 cut(s)	2130
Eco130I	CCWWGG	4 cut(s)	1378, 1488, 2362, 2418
Eco24I	GRGCYC	2 cut(s)	2400, 2417
Eco31I	GGTCTC	1 cut(s)	209
Eco32I	GATATC	1 cut(s)	924
Eco53kI	GAGCTC	2 cut(s)	2398, 2415
EcoICRI	GAGCTC	2 cut(s)	2398, 2415
EcoNI	CCTNNNNNAGG	1 cut(s)	2261
EcoRI	GAATTC	2 cut(s)	1071, 2582
EcoRII	CCWGG	3 cut(s)	195, 1111, 1210
EcoRV	GATATC	1 cut(s)	924
EcoT14I	CCWWGG	4 cut(s)	1378, 1488, 2362, 2418
EcoT22I	ATGCAT	2 cut(s)	505, 1684
EcoT38I	GRGCYC	2 cut(s)	2400, 2417
ErhI	CCWWGG	4 cut(s)	1378, 1488, 2362, 2418
FaqI	GGGAC	2 cut(s)	876, 1841
FauI	CCCGC	1 cut(s)	451
FauNDI	CATATG	2 cut(s)	452, 2936
FbaI	TGATCA	2 cut(s)	1086, 1888
FblI	GTMKAC	4 cut(s)	809, 1551, 2126, 2762
Fnu4HI	GCNGC	3 cut(s)	127, 628, 2708
FriOI	GRGCYC	2 cut(s)	2400, 2417
Fsp4HI	GCNGC	3 cut(s)	127, 628, 2708
FspBI	CTAG	3 cut(s)	1107, 1283, 1446
GlaI	GCGC	3 cut(s)	254, 759, 983
GluI	GCNGC	3 cut(s)	127, 628, 2708
GsaI	CCCAGC	1 cut(s)	849
GsuI	CTGGAG	5 cut(s)	46, 127, 539, 1821, 2723
HapII	CCGG	5 cut(s)	167, 212, 548, 1207, 1971
HgaI	GACGC	1 cut(s)	302
HhaI	GCGC	3 cut(s)	255, 760, 984
Hin1I	GRCGYC	1 cut(s)	294
Hin6I	GCGC	3 cut(s)	253, 758, 982
HinP1I	GCGC	3 cut(s)	253, 758, 982
HincII	GTYRAC	1 cut(s)	1737
HindII	GTYRAC	1 cut(s)	1737
HindIII	AAGCTT	4 cut(s)	1299, 2063, 2283, 2291
HpaII	CCGG	5 cut(s)	167, 212, 548, 1207, 1971
HphI	GGTGA	5 cut(s)	587, 1319, 1985, 2211, 2434
Hpy188I	TCNGA	9 cut(s)	331, 384, 1196, 1611, 1893, 1964, 2101, 2929, 2959
Hpy99I	CGWCG	2 cut(s)	182, 296
HpyAV	CCTTC	9 cut(s)	91, 850, 874, 1088, 1693, 1994, 2561, 2975, 2981
HpyCH4III	ACNGT	4 cut(s)	904, 1535, 2555, 2983
HpyCH4IV	ACGT	3 cut(s)	1653, 2129, 2331
HpyF10VI	GCNNNNNNNGC	8 cut(s)	259, 627, 670, 1212, 1298, 1476, 2798, 2807
HpyF3I	CTNAG	6 cut(s)	362, 429, 593, 1076, 1273, 1584
HpySE526I	ACGT	3 cut(s)	1653, 2129, 2331
Hsp92I	GRCGYC	1 cut(s)	294
HspAI	GCGC	3 cut(s)	253, 758, 982
KroI	GCCGGC	1 cut(s)	1206
KroNI	GCCGGC	1 cut(s)	1208
Ksp22I	TGATCA	2 cut(s)	1086, 1888
Kzo9I	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
LguI	GCTCTTC	2 cut(s)	654, 2922
LmnI	GCTCC	4 cut(s)	1925, 2420, 2434, 2704
Lsp1109I	GCAGC	2 cut(s)	614, 2694
LweI	GCATC	4 cut(s)	1302, 1699, 1975, 2020
MaeI	CTAG	3 cut(s)	1107, 1283, 1446
MaeII	ACGT	3 cut(s)	1653, 2129, 2331
MaeIII	GTNAC	7 cut(s)	513, 536, 1009, 1657, 1973, 2335, 2570
MalI	GATC	7 cut(s)	192, 1088, 1150, 1781, 1890, 2175, 2774
MboI	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
MfeI	CAATTG	1 cut(s)	2475
MflI	RGATCY	1 cut(s)	2173
MhlI	GDGCHC	5 cut(s)	897, 1648, 2400, 2417, 2431
MlsI	TGGCCA	3 cut(s)	1377, 1788, 2533
MluNI	TGGCCA	3 cut(s)	1377, 1788, 2533
MlyI	GAGTC	7 cut(s)	251, 421, 433, 706, 1732, 2621, 2954
MmeI	TCCRAC	3 cut(s)	489, 762, 2248
Mox20I	TGGCCA	3 cut(s)	1377, 1788, 2533
Mph1103I	ATGCAT	2 cut(s)	505, 1684
MroNI	GCCGGC	1 cut(s)	1206
MroXI	GAANNNNTTC	1 cut(s)	2916
MscI	TGGCCA	3 cut(s)	1377, 1788, 2533
MslI	CAYNNNNRTG	3 cut(s)	1920, 2348, 2469
Msp20I	TGGCCA	3 cut(s)	1377, 1788, 2533
MspCI	CTTAAG	1 cut(s)	674
MspI	CCGG	5 cut(s)	167, 212, 548, 1207, 1971
MspR9I	CCNGG	3 cut(s)	197, 1113, 1212
MunI	CAATTG	1 cut(s)	2475
Mva1269I	GAATGC	3 cut(s)	984, 996, 1749
MvaI	CCWGG	3 cut(s)	197, 1113, 1212
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	8 cut(s)	259, 627, 670, 1212, 1298, 1476, 2798, 2807
NaeI	GCCGGC	1 cut(s)	1208
NcoI	CCATGG	1 cut(s)	1378
NdeI	CATATG	2 cut(s)	452, 2936
NdeII	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
NgoMIV	GCCGGC	1 cut(s)	1206
NlaIV	GGNNCC	2 cut(s)	1205, 1704
NmeAIII	GCCGAG	1 cut(s)	720
NmuCI	GTSAC	3 cut(s)	536, 1973, 2335
NsiI	ATGCAT	2 cut(s)	505, 1684
NspI	RCATGY	2 cut(s)	1682, 2792
PaqCI	CACCTGC	2 cut(s)	394, 1050
PasI	CCCWGGG	1 cut(s)	1112
PciSI	GCTCTTC	2 cut(s)	654, 2922
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	3 cut(s)	984, 996, 1749
PdiI	GCCGGC	1 cut(s)	1208
PdmI	GAANNNNTTC	1 cut(s)	2916
PfeI	GAWTC	3 cut(s)	147, 332, 616
PkrI	GCNGC	3 cut(s)	128, 629, 2709
PleI	GAGTC	7 cut(s)	251, 420, 432, 705, 1732, 2621, 2954
PpsI	GAGTC	7 cut(s)	251, 420, 432, 705, 1732, 2621, 2954
Ppu21I	YACGTR	1 cut(s)	2130
PshAI	GACNNNNGTC	1 cut(s)	2388
PshBI	ATTAAT	1 cut(s)	2898
PsiI	TTATAA	2 cut(s)	1173, 2289
Psp124BI	GAGCTC	2 cut(s)	2400, 2417
Psp1406I	AACGTT	1 cut(s)	2331
Psp6I	CCWGG	3 cut(s)	195, 1111, 1210
PspFI	CCCAGC	1 cut(s)	845
PspGI	CCWGG	3 cut(s)	195, 1111, 1210
PspN4I	GGNNCC	2 cut(s)	1205, 1704
PstI	CTGCAG	1 cut(s)	2817
PsuI	RGATCY	1 cut(s)	2173
RsaI	GTAC	6 cut(s)	546, 689, 1450, 2132, 2863, 2985
RsaNI	GTAC	6 cut(s)	545, 688, 1449, 2131, 2862, 2984
RseI	CAYNNNNRTG	3 cut(s)	1920, 2348, 2469
SacI	GAGCTC	2 cut(s)	2400, 2417
SapI	GCTCTTC	2 cut(s)	654, 2922
SatI	GCNGC	3 cut(s)	127, 628, 2708
Sau3AI	GATC	7 cut(s)	190, 1086, 1148, 1779, 1888, 2173, 2772
SchI	GAGTC	7 cut(s)	251, 421, 433, 706, 1732, 2621, 2954
ScrFI	CCNGG	3 cut(s)	197, 1113, 1212
SduI	GDGCHC	5 cut(s)	897, 1648, 2400, 2417, 2431
SfaNI	GCATC	4 cut(s)	1302, 1699, 1975, 2020
SfcI	CTRYAG	2 cut(s)	1015, 2813
SmiMI	CAYNNNNRTG	3 cut(s)	1920, 2348, 2469
SmlI	CTYRAG	3 cut(s)	674, 1740, 2393
SmoI	CTYRAG	3 cut(s)	674, 1740, 2393
SnaBI	TACGTA	1 cut(s)	2130
SpeI	ACTAGT	1 cut(s)	1445
SsiI	CCGC	4 cut(s)	127, 164, 231, 458
SspMI	CTAG	3 cut(s)	1107, 1283, 1446
SstI	GAGCTC	2 cut(s)	2400, 2417
StyD4I	CCNGG	3 cut(s)	195, 1111, 1210
StyI	CCWWGG	4 cut(s)	1378, 1488, 2362, 2418
TaaI	ACNGT	4 cut(s)	904, 1535, 2555, 2983
TaiI	ACGT	3 cut(s)	1656, 2132, 2334
TaqI	TCGA	6 cut(s)	177, 189, 1782, 2250, 2382, 2775
TaqII	GACCGA	1 cut(s)	815
TatI	WGTACW	3 cut(s)	1448, 2861, 2983
TauI	GCSGC	1 cut(s)	129
TfiI	GAWTC	3 cut(s)	147, 332, 616
TscAI	CASTG	2 cut(s)	2350, 2817
TseFI	GTSAC	3 cut(s)	536, 1973, 2335
TseI	GCWGC	2 cut(s)	627, 2707
Tsp45I	GTSAC	3 cut(s)	536, 1973, 2335
TspGWI	ACGGA	2 cut(s)	481, 1106
TspRI	CASTG	2 cut(s)	2350, 2817
Vha464I	CTTAAG	1 cut(s)	674
VneI	GTGCAC	1 cut(s)	1644
VspI	ATTAAT	1 cut(s)	2898
XagI	CCTNNNNNAGG	1 cut(s)	2261
XapI	RAATTY	3 cut(s)	1071, 1385, 2582
XceI	RCATGY	2 cut(s)	1682, 2792
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1796
XmiI	GTMKAC	4 cut(s)	809, 1551, 2126, 2762
XmnI	GAANNNNTTC	1 cut(s)	2916
XspI	CTAG	3 cut(s)	1107, 1283, 1446
Zsp2I	ATGCAT	2 cut(s)	505, 1684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.