Rh4AG150300

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003234
Physical Location & Seq
Reverse (-)
37863954 .. 37869663
5710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG150300.1

Sequence Viewer

Length: 2961 bp
ATGAGCAGAAGACACTTCAGCTCTATACTCTTCAACTCCATCTCTTCTTCTCCCCTTTTCTCCAGAAACTCATCCCAATCCTTCGTTTCTTCACTCAAATCATACCCCCAATGTCTCTTCTCCAGATTCCTCTGCACCTCTCCGCCGGACAATCTCGACGGCCTCCTCGATCCCCAGGACTTCTCTACGCCGGAGACCCACTCTCTTTCCGCACAAGACTTCGCCTTTTTGCGCGACTCGCCGAAACTTGACCCCGATAAGTGTTCTAACGACGCCGTTTTGATTTCTAATGCCATTAGGAATAGTTCTGATTCATTTGGGGATGAAACCCACAAGTTTCTTAGGAGGTTTAGGGATAATCTGAATGAAACTTTGGTGGTGCAGGTGTTGAGTCTTGTAAAGAGTCCTGAGTTGGTTGTCAAGTTTTTCATTTGGGCAGGTAGGCAAATTGGGTATAGTCATACTGCTTCTGTATATAATGCATTGTTGGAGTTACTGGAGTGTGGTAGTAGCGACAGAGTACCGGAGCATTTTTTGCGAGAAATTATGGGTGATGATAGAGAGGTGCTTGGGAAGTTGCTTAATGTGTTGATTCGCAAGTGTTGCCGAAATGGTTTGTGGAATGTAGCTTTGGAAGAGCTTGGGAGGCTTAAGGATTTCGGGTACAGGCCGAGTCAAGCAACTTACAATGCATTGGTTCAAGTGTTTTTGAGAGCTGATAGGCTGGACACAGCGCACTTGGTTCATGGGGAGATGTTGGAGTTGGGGTTTAAGATGGATGAGTATACGCTAGGTTGTTTTGGTCATGCCCTGTGTAAAGCTGGGAGGTGGAAGGAGGGACTTGCATTGATTGAGAAGGAAGAGTTTGTTCCCGATACTGTTCTTTATACCAAGATGATTTCTGGGTTGTGTGAGGCTTCACTTTTTGAGGAAGCTATGGATTTGTTGAGCAGAATGCGCTGTAATTCTTGCATTCCTAATGTTGTAACCTATAGGATTTTGCTTTGTGGATGTTTGAAGAAAAAACAGCTTGGCAGATCAGGAGATTACTCTTATGCTTATAAGTTGCTAAAGAAAATGGTCAGGTGTGGGTGCTGGCCAGGCTATGTGGTTTACAATATCTTGATTGGTAGTATCTGTGGACACGAAGAATTACCTACCTTAGATATGCTAGACATGGCTGAAAAAGCTTATGGTGAGATGCTTAATGCAGGGGTTGTTTTAAATAAGGTAAATGTAAGCAATTTTGTGCGATGTCTTTGTGGCCATGGAAAATTTGACAAAGCCTACAAGGTCATCAATGAAATGATGAGCAAGGGTTTTGTTCCTGACACTAGTACATACTCTAAAGTTATTGGCTTTTTGTGCAATGCCTCCAAGGTAGAACAAGCATTATTGTTGTTTGAAGAAATGAAGCAAAACGGTGTTGTTCCTGATGTCTACACCTATACCATCTTAATTGATAGTTTTTCTAAGGCTGGCCTTATTGAACAGGCTCGGAACTGGTTTAATGAAATGGTAGGAAATGGTTGTGCACCAAACGTGGTAACTTATACTGCTCTTATACATGCATACCTTAAAGCAAGGAAGGTGCCTGATGCAAATCAACTCTTTGAGATGATGTTGACTCAAGGTTGCATTCCTAATGCTATTACTTATAGTGCTTTGATCGATGGCCATTGTAAAGCTGGAGAGACTGAAAAGGCTTGCCTGATTTATGCAAGAATGAGAGGTAATGTGGAAGTCCCTGATGTTGATATGTATTTCAAAATTGATGATCAGAGTTTGAAAGAACCTAATGTTCATACTTATGGAGCGTTGGTGGATGGTTTATGTAAAGCCCACAAGGTCAGAGAGGCCGGTGACTTATTGGATGCTATGTTGGTAGAAGGTTGTGAACCTAACCACATTGTTTATGATGCTCTTATAGATGGATTTTGCAAGTCTGGGAAGCTCGATGAAGCACAAAAGGTGTTTGCTAAAATGTCAGAGCATGGGTATAGTCCAAATGTGTATACGTACAGCTCTTTGATTGACAGGTTGTTCAAGGATAAAAGACTGGATCTTGTTTTAAAAGTCCTGTCTAAAATGTTAGAAAACTCTTGTTCACCAAATGTTGTTATCTATACAGAGATGGTCGATGGCCTTTGTAAGGTTGGAAAGACAGATGAAGCTTATAAGCTTATGCTAATGATGGAAGAAAAAGGATGTAATCCAAATGTTGTGACTTACACTGCGATGATAGATGGCCTTGGGAAAGCAGGCAAAATTGACAAATGTCTTGAGCTCTTTAAAGCAATGAGCTCCAAGGGTTGTGCTCCCAATTTTATCACCTACAAGGTTTTGATAAACCATTGCTGTGCAATTGGTCTTTTGGATGAAGCTCATAAACTTTTGGATGAAATGAAACAAACATATTGGCCAAAGCATTTGGCAGGCTACCGTAAAGTTATTGAAGGTTACAACCGAGAATTCATAGCCTCACTTGGGCTTTTAAATGAGATAAGTGAGTGCGACTCATTGCCAATCGTTCACATATACAGAGTTTTGGTTGATAATTTTGTAAAGGCTGGAAGACTGGAAGTGGCTCTGGAGCTGCATGAAGAGATTTCTTCATCTACTCCTTTTACATCTGTGAACAAGGATATGTATACTTTACTGATCGAGAACCTATCACATGCAAATAAGGCTGATAAGGCACTGCAGATGTTTGCAGAAATGATAAGGCTGGGTGGTTATCCAGAGCTGAGTACATTTTTCCACCTCATCAAAGGGCTTATTAAAATTAATAGGTGGGATGAAGCTCTTCAACTATCGGATAGCATATGCCAGATGTTCAAGCACTCACAAGCACCTAATTATGAGTTAAAACGTGTGCCCTATGTCAGTTTGAGGGGAGATTGCAAATCACCATTTCTGATTTTCTGCCTTGTCGTTTCACCTACTGGTCTCTATGCTTGA

Protein Analysis

986

Amino Acids

110.81

Weight (kDa)

6.35

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 224 - 273 3.9e-06 PPR repeat family
PPR_1 PF12854 289 - 320 2.5e-06 PPR repeat
PPR_2 PF13041 291 - 340 4.4e-14 PPR repeat family
PPR_long PF17177 345 - 494 5.7e-10 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 416 - 457 7.3e-09 PPR repeat family
PPR_3 PF13812 431 - 490 4.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 443 - 492 6.9e-16 PPR repeat family
PPR_long PF17177 461 - 579 1.1e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 474 - 507 5.8e-09 PPR repeat
PPR PF01535 481 - 511 2.4e-07 PPR repeat
PPR_2 PF13041 486 - 525 2.3e-10 PPR repeat family
PPR_3 PF13812 503 - 558 1.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 509 - 541 4.5e-07 PPR repeat
PPR_2 PF13041 513 - 562 5e-17 PPR repeat family
PPR PF01535 516 - 546 8.1e-06 PPR repeat
PPR_1 PF12854 545 - 576 7.5e-09 PPR repeat
PPR_1 PF12854 598 - 627 7.8e-07 PPR repeat
PPR_2 PF13041 599 - 639 8.3e-08 PPR repeat family
PPR_3 PF13812 623 - 679 4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 631 - 663 1.2e-12 PPR repeat
PPR_2 PF13041 636 - 683 6.3e-15 PPR repeat family
PPR PF01535 638 - 666 4.6e-07 PPR repeat
PPR_3 PF13812 657 - 710 1.1e-08 Pentatricopeptide repeat domain
PPR_long PF17177 685 - 807 3e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 693 - 752 3.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 701 - 732 1.4e-08 PPR repeat
PPR_2 PF13041 704 - 753 1.2e-16 PPR repeat family
PPR_3 PF13812 731 - 786 1.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 735 - 767 1.5e-10 PPR repeat
PPR_2 PF13041 739 - 787 6e-17 PPR repeat family
PPR PF01535 742 - 772 3.1e-09 PPR repeat
PPR_1 PF12854 770 - 803 1.1e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1062, 2178
AarI CACCTGC 1 cut(s) 373
Acc36I ACCTGC 2 cut(s) 373, 428
AccB1I GGYRCC 1 cut(s) 1591
AccI GTMKAC 4 cut(s) 785, 1440, 2015, 2651
AccII CGCG 1 cut(s) 234
AciI CCGC 2 cut(s) 143, 210
AclWI GGATC 2 cut(s) 164, 2070
AcoI YGGCCR 4 cut(s) 1097, 1264, 1675, 2420
AcsI RAATTY 2 cut(s) 1274, 2471
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 5 cut(s) 522, 665, 1339, 2021, 2752
AfiI CCNNNNNNNGG 2 cut(s) 2152, 2487
AflII CTTAAG 1 cut(s) 650
AflIII ACRYGT 1 cut(s) 2872
AhlI ACTAGT 1 cut(s) 1334
AjnI CCWGG 2 cut(s) 174, 1099
AjuI GAANNNNNNNTTGG 4 cut(s) 356, 388, 614, 646
Alw21I GWGCWC 4 cut(s) 1537, 2289, 2306, 2320
Alw26I GTCTC 4 cut(s) 119, 188, 1688, 2954
Alw44I GTGCAC 1 cut(s) 1533
AlwI GGATC 2 cut(s) 164, 2070
ApaLI GTGCAC 1 cut(s) 1533
ApeKI GCWGC 1 cut(s) 2596
ApoI RAATTY 2 cut(s) 1274, 2471
AseI ATTAAT 1 cut(s) 2787
Asp700I GAANNNNTTC 1 cut(s) 2805
AspLEI GCGC 3 cut(s) 234, 736, 960
AsuHPI GGTGA 7 cut(s) 563, 1208, 1874, 2100, 2323, 2901, 2931
BaeGI GKGCMC 2 cut(s) 1537, 2880
BalI TGGCCA 4 cut(s) 1099, 1266, 1677, 2422
BanI GGYRCC 1 cut(s) 1591
BanII GRGCYC 2 cut(s) 2289, 2306
BbsI GAAGAC 2 cut(s) 16, 2581
Bbv12I GWGCWC 4 cut(s) 1537, 2289, 2306, 2320
BbvI GCAGC 1 cut(s) 2583
BceAI ACGGC 2 cut(s) 175, 260
BciT130I CCWGG 2 cut(s) 176, 1101
BclI TGATCA 1 cut(s) 1777
BcoDI GTCTC 4 cut(s) 119, 188, 1688, 2954
BcuI ACTAGT 1 cut(s) 1334
BfaI CTAG 3 cut(s) 791, 1172, 1335
BfmI CTRYAG 2 cut(s) 991, 2702
BfrI CTTAAG 1 cut(s) 650
BfuAI ACCTGC 2 cut(s) 373, 428
BisI GCNGC 1 cut(s) 2597
BlsI GCNGC 1 cut(s) 2598
Bme1390I CCNGG 2 cut(s) 176, 1101
BmiI GGNNCC 1 cut(s) 1593
BmrFI CCNGG 2 cut(s) 176, 1101
BmsI GCATC 4 cut(s) 1191, 1588, 1864, 1909
BoxI GACNNNNGTC 1 cut(s) 2277
BpiI GAAGAC 2 cut(s) 16, 2581
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 2501, 2533
BpmI CTGGAG 5 cut(s) 46, 106, 518, 1710, 2612
BpuEI CTTGAG 2 cut(s) 1614, 2303
Bsa29I ATCGAT 1 cut(s) 1671
BsaAI YACGTR 1 cut(s) 2019
BsaBI GATNNNNATC 1 cut(s) 1602
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 2 cut(s) 188, 2954
BsaJI CCNNGG 5 cut(s) 174, 1267, 1377, 2251, 2307
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 4 cut(s) 752, 782, 1806, 1836
Bsc4I CCNNNNNNNGG 2 cut(s) 2152, 2487
Bse118I RCCGGY 1 cut(s) 1859
Bse1I ACTGG 5 cut(s) 501, 1508, 2064, 2583, 2950
Bse3DI GCAATG 4 cut(s) 1375, 2304, 2353, 2519
Bse8I GATNNNNATC 1 cut(s) 1602
BseBI CCWGG 2 cut(s) 176, 1101
BseCI ATCGAT 1 cut(s) 1671
BseDI CCNNGG 5 cut(s) 174, 1267, 1377, 2251, 2307
BseJI GATNNNNATC 1 cut(s) 1602
BseLI CCNNNNNNNGG 2 cut(s) 2152, 2487
BseMI GCAATG 4 cut(s) 1375, 2304, 2353, 2519
BseMII CTCAG 2 cut(s) 399, 2738
BseNI ACTGG 5 cut(s) 501, 1508, 2064, 2583, 2950
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 2 cut(s) 1537, 2880
BseXI GCAGC 1 cut(s) 2583
BseYI CCCAGC 2 cut(s) 821, 2728
BsgI GTGCAG 2 cut(s) 118, 401
Bsh1236I CGCG 1 cut(s) 234
BshNI GGYRCC 1 cut(s) 1591
BshVI ATCGAT 1 cut(s) 1671
BsiHKAI GWGCWC 4 cut(s) 1537, 2289, 2306, 2320
BsiSI CCGG 4 cut(s) 146, 191, 524, 1860
BslFI GGGAC 2 cut(s) 852, 1730
BslI CCNNNNNNNGG 2 cut(s) 2152, 2487
BsmAI GTCTC 4 cut(s) 119, 188, 1688, 2954
BsmFI GGGAC 2 cut(s) 852, 1730
BsmI GAATGC 3 cut(s) 960, 972, 1638
Bso31I GGTCTC 2 cut(s) 188, 2954
Bsp1286I GDGCHC 5 cut(s) 1537, 2289, 2306, 2320, 2880
Bsp143I GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
Bsp19I CCATGG 1 cut(s) 1267
BspACI CCGC 2 cut(s) 143, 210
BspCNI CTCAG 2 cut(s) 400, 2739
BspDI ATCGAT 1 cut(s) 1671
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 1593
BspMAI CTGCAG 1 cut(s) 2706
BspMI ACCTGC 2 cut(s) 373, 428
BspPI GGATC 2 cut(s) 164, 2070
BspQI GCTCTTC 2 cut(s) 630, 2811
BspT107I GGYRCC 1 cut(s) 1591
BspTI CTTAAG 1 cut(s) 650
BspTNI GGTCTC 2 cut(s) 188, 2954
BsrDI GCAATG 4 cut(s) 1375, 2304, 2353, 2519
BsrFI RCCGGY 1 cut(s) 1859
BsrI ACTGG 5 cut(s) 501, 1508, 2064, 2583, 2950
BssAI RCCGGY 1 cut(s) 1859
BssECI CCNNGG 5 cut(s) 174, 1267, 1377, 2251, 2307
BssMI GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
BssNAI GTATAC 3 cut(s) 786, 2016, 2652
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 4 cut(s) 1267, 1377, 2251, 2307
Bst1107I GTATAC 3 cut(s) 786, 2016, 2652
Bst2UI CCWGG 2 cut(s) 176, 1101
Bst4CI ACNGT 3 cut(s) 880, 1424, 2444
Bst6I CTCTTC 7 cut(s) 35, 49, 122, 630, 855, 2598, 2811
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 650
BstAPI GCANNNNNTGC 2 cut(s) 535, 603
BstBAI YACGTR 1 cut(s) 2019
BstC8I GCNNGC 5 cut(s) 1097, 1480, 1708, 2263, 2437
BstDEI CTNAG 5 cut(s) 341, 408, 1162, 1473, 2747
BstDSI CCRYGG 1 cut(s) 1267
BstENI CCTNNNNNAGG 1 cut(s) 2150
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 3 cut(s) 234, 736, 960
BstKTI GATC 6 cut(s) 172, 1040, 1671, 1780, 2065, 2664
BstMAI GTCTC 4 cut(s) 119, 188, 1688, 2954
BstMBI GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
BstNI CCWGG 2 cut(s) 176, 1101
BstNSI RCATGY 2 cut(s) 1571, 2681
BstPAI GACNNNNGTC 1 cut(s) 2277
BstSCI CCNGG 2 cut(s) 174, 1099
BstSFI CTRYAG 2 cut(s) 991, 2702
BstSLI GKGCMC 2 cut(s) 1537, 2880
BstSNI TACGTA 1 cut(s) 2019
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 2583
BstV2I GAAGAC 2 cut(s) 16, 2581
BstX2I RGATCY 1 cut(s) 2062
BstYI RGATCY 1 cut(s) 2062
BstZ17I GTATAC 3 cut(s) 786, 2016, 2652
Bsu15I ATCGAT 1 cut(s) 1671
BsuTUI ATCGAT 1 cut(s) 1671
BtgI CCRYGG 1 cut(s) 1267
BtgZI GCGATG 2 cut(s) 1267, 2252
BtsI GCAGTG 2 cut(s) 2232, 2699
BtsIMutI CAGTG 2 cut(s) 2232, 2699
BveI ACCTGC 2 cut(s) 373, 428
Cac8I GCNNGC 5 cut(s) 1097, 1480, 1708, 2263, 2437
CfoI GCGC 3 cut(s) 234, 736, 960
Cfr10I RCCGGY 1 cut(s) 1859
ClaI ATCGAT 1 cut(s) 1671
CseI GACGC 1 cut(s) 281
Csp6I GTAC 5 cut(s) 521, 664, 1338, 2020, 2751
CviAII CATG 8 cut(s) 746, 806, 1177, 1268, 1568, 1994, 2600, 2678
CviQI GTAC 5 cut(s) 521, 664, 1338, 2020, 2751
DdeI CTNAG 5 cut(s) 341, 408, 1162, 1473, 2747
DpnI GATC 6 cut(s) 171, 1039, 1670, 1779, 2064, 2663
DpnII GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
DraI TTTAAA 4 cut(s) 1224, 2073, 2293, 2496
EaeI YGGCCR 4 cut(s) 1097, 1264, 1675, 2420
Eam1104I CTCTTC 7 cut(s) 35, 49, 122, 630, 855, 2598, 2811
EarI CTCTTC 7 cut(s) 35, 49, 122, 630, 855, 2598, 2811
EciI GGCGGA 1 cut(s) 132
Ecl136II GAGCTC 2 cut(s) 2287, 2304
Eco105I TACGTA 1 cut(s) 2019
Eco130I CCWWGG 4 cut(s) 1267, 1377, 2251, 2307
Eco24I GRGCYC 2 cut(s) 2289, 2306
Eco31I GGTCTC 2 cut(s) 188, 2954
Eco53kI GAGCTC 2 cut(s) 2287, 2304
EcoICRI GAGCTC 2 cut(s) 2287, 2304
EcoNI CCTNNNNNAGG 1 cut(s) 2150
EcoRI GAATTC 1 cut(s) 2471
EcoRII CCWGG 2 cut(s) 174, 1099
EcoT14I CCWWGG 4 cut(s) 1267, 1377, 2251, 2307
EcoT22I ATGCAT 3 cut(s) 484, 694, 1573
EcoT38I GRGCYC 2 cut(s) 2289, 2306
ErhI CCWWGG 4 cut(s) 1267, 1377, 2251, 2307
FaeI CATG 8 cut(s) 749, 809, 1180, 1271, 1571, 1997, 2603, 2681
FaqI GGGAC 2 cut(s) 852, 1730
FatI CATG 8 cut(s) 745, 805, 1176, 1267, 1567, 1993, 2599, 2677
FauNDI CATATG 1 cut(s) 2825
FbaI TGATCA 1 cut(s) 1777
FblI GTMKAC 4 cut(s) 785, 1440, 2015, 2651
Fnu4HI GCNGC 1 cut(s) 2597
FriOI GRGCYC 2 cut(s) 2289, 2306
Fsp4HI GCNGC 1 cut(s) 2597
FspBI CTAG 3 cut(s) 791, 1172, 1335
GlaI GCGC 3 cut(s) 233, 735, 959
GluI GCNGC 1 cut(s) 2597
GsaI CCCAGC 2 cut(s) 825, 2732
GsuI CTGGAG 5 cut(s) 46, 106, 518, 1710, 2612
HapII CCGG 4 cut(s) 146, 191, 524, 1860
HgaI GACGC 1 cut(s) 281
HhaI GCGC 3 cut(s) 234, 736, 960
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 8 cut(s) 749, 809, 1180, 1271, 1571, 1997, 2603, 2681
Hin6I GCGC 3 cut(s) 232, 734, 958
HinP1I GCGC 3 cut(s) 232, 734, 958
HincII GTYRAC 1 cut(s) 1626
HindII GTYRAC 1 cut(s) 1626
HindIII AAGCTT 3 cut(s) 1188, 2172, 2180
HinfI GANTC 9 cut(s) 126, 236, 311, 391, 403, 592, 673, 1627, 2516
HpaII CCGG 4 cut(s) 146, 191, 524, 1860
HphI GGTGA 7 cut(s) 563, 1208, 1874, 2100, 2323, 2901, 2931
Hpy188I TCNGA 8 cut(s) 310, 363, 1500, 1782, 1853, 1990, 2818, 2919
Hpy99I CGWCG 2 cut(s) 161, 275
HpyAV CCTTC 6 cut(s) 91, 826, 850, 1582, 1883, 2450
HpyCH4III ACNGT 3 cut(s) 880, 1424, 2444
HpyCH4IV ACGT 3 cut(s) 1542, 2018, 2872
HpyF3I CTNAG 5 cut(s) 341, 408, 1162, 1473, 2747
HpySE526I ACGT 3 cut(s) 1542, 2018, 2872
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 8 cut(s) 749, 809, 1180, 1271, 1571, 1997, 2603, 2681
HspAI GCGC 3 cut(s) 232, 734, 958
Ksp22I TGATCA 1 cut(s) 1777
Kzo9I GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
LguI GCTCTTC 2 cut(s) 630, 2811
LmnI GCTCC 5 cut(s) 526, 1814, 2309, 2323, 2593
Lsp1109I GCAGC 1 cut(s) 2583
LweI GCATC 4 cut(s) 1191, 1588, 1864, 1909
MaeI CTAG 3 cut(s) 791, 1172, 1335
MaeII ACGT 3 cut(s) 1542, 2018, 2872
MaeIII GTNAC 6 cut(s) 492, 985, 1546, 1862, 2224, 2459
MalI GATC 6 cut(s) 171, 1039, 1670, 1779, 2064, 2663
MboI GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
MfeI CAATTG 1 cut(s) 2364
MflI RGATCY 1 cut(s) 2062
MhlI GDGCHC 5 cut(s) 1537, 2289, 2306, 2320, 2880
MlsI TGGCCA 4 cut(s) 1099, 1266, 1677, 2422
MluNI TGGCCA 4 cut(s) 1099, 1266, 1677, 2422
MlyI GAGTC 6 cut(s) 230, 400, 412, 682, 1621, 2510
MmeI TCCRAC 3 cut(s) 468, 738, 2137
Mox20I TGGCCA 4 cut(s) 1099, 1266, 1677, 2422
Mph1103I ATGCAT 3 cut(s) 484, 694, 1573
MroXI GAANNNNTTC 1 cut(s) 2805
MscI TGGCCA 4 cut(s) 1099, 1266, 1677, 2422
MslI CAYNNNNRTG 3 cut(s) 1809, 2237, 2358
Msp20I TGGCCA 4 cut(s) 1099, 1266, 1677, 2422
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 4 cut(s) 146, 191, 524, 1860
MspR9I CCNGG 2 cut(s) 176, 1101
MunI CAATTG 1 cut(s) 2364
Mva1269I GAATGC 3 cut(s) 960, 972, 1638
MvaI CCWGG 2 cut(s) 176, 1101
MvnI CGCG 1 cut(s) 234
NcoI CCATGG 1 cut(s) 1267
NdeI CATATG 1 cut(s) 2825
NdeII GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
NlaIII CATG 8 cut(s) 749, 809, 1180, 1271, 1571, 1997, 2603, 2681
NlaIV GGNNCC 1 cut(s) 1593
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 2 cut(s) 1862, 2224
NsiI ATGCAT 3 cut(s) 484, 694, 1573
NspI RCATGY 2 cut(s) 1571, 2681
PaqCI CACCTGC 1 cut(s) 373
PciSI GCTCTTC 2 cut(s) 630, 2811
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 3 cut(s) 960, 972, 1638
PdmI GAANNNNTTC 1 cut(s) 2805
PfeI GAWTC 3 cut(s) 126, 311, 592
PkrI GCNGC 1 cut(s) 2598
PleI GAGTC 6 cut(s) 230, 399, 411, 681, 1621, 2510
PpsI GAGTC 6 cut(s) 230, 399, 411, 681, 1621, 2510
Ppu21I YACGTR 1 cut(s) 2019
PshAI GACNNNNGTC 1 cut(s) 2277
PshBI ATTAAT 1 cut(s) 2787
PsiI TTATAA 2 cut(s) 1062, 2178
Psp124BI GAGCTC 2 cut(s) 2289, 2306
Psp6I CCWGG 2 cut(s) 174, 1099
PspFI CCCAGC 2 cut(s) 821, 2728
PspGI CCWGG 2 cut(s) 174, 1099
PspN4I GGNNCC 1 cut(s) 1593
PstI CTGCAG 1 cut(s) 2706
PsuI RGATCY 1 cut(s) 2062
RsaI GTAC 5 cut(s) 522, 665, 1339, 2021, 2752
RsaNI GTAC 5 cut(s) 521, 664, 1338, 2020, 2751
RseI CAYNNNNRTG 3 cut(s) 1809, 2237, 2358
SacI GAGCTC 2 cut(s) 2289, 2306
SapI GCTCTTC 2 cut(s) 630, 2811
SatI GCNGC 1 cut(s) 2597
Sau3AI GATC 6 cut(s) 169, 1037, 1668, 1777, 2062, 2661
SchI GAGTC 6 cut(s) 230, 400, 412, 682, 1621, 2510
ScrFI CCNGG 2 cut(s) 176, 1101
SduI GDGCHC 5 cut(s) 1537, 2289, 2306, 2320, 2880
SfaNI GCATC 4 cut(s) 1191, 1588, 1864, 1909
SfcI CTRYAG 2 cut(s) 991, 2702
SmiMI CAYNNNNRTG 3 cut(s) 1809, 2237, 2358
SmlI CTYRAG 3 cut(s) 650, 1629, 2282
SmoI CTYRAG 3 cut(s) 650, 1629, 2282
SnaBI TACGTA 1 cut(s) 2019
SpeI ACTAGT 1 cut(s) 1334
SsiI CCGC 2 cut(s) 143, 210
SspMI CTAG 3 cut(s) 791, 1172, 1335
SstI GAGCTC 2 cut(s) 2289, 2306
StyD4I CCNGG 2 cut(s) 174, 1099
StyI CCWWGG 4 cut(s) 1267, 1377, 2251, 2307
TaaI ACNGT 3 cut(s) 880, 1424, 2444
TaiI ACGT 3 cut(s) 1545, 2021, 2875
TaqI TCGA 6 cut(s) 156, 168, 1671, 1956, 2139, 2664
TatI WGTACW 2 cut(s) 1337, 2750
TfiI GAWTC 3 cut(s) 126, 311, 592
TscAI CASTG 2 cut(s) 2239, 2706
TseFI GTSAC 2 cut(s) 1862, 2224
TseI GCWGC 1 cut(s) 2596
Tsp45I GTSAC 2 cut(s) 1862, 2224
TspRI CASTG 2 cut(s) 2239, 2706
Vha464I CTTAAG 1 cut(s) 650
VneI GTGCAC 1 cut(s) 1533
VspI ATTAAT 1 cut(s) 2787
XagI CCTNNNNNAGG 1 cut(s) 2150
XapI RAATTY 2 cut(s) 1274, 2471
XceI RCATGY 2 cut(s) 1571, 2681
XcmI CCANNNNNNNNNTGG 1 cut(s) 1685
XmiI GTMKAC 4 cut(s) 785, 1440, 2015, 2651
XmnI GAANNNNTTC 1 cut(s) 2805
XspI CTAG 3 cut(s) 791, 1172, 1335
Zsp2I ATGCAT 3 cut(s) 484, 694, 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.