MD04G1034700.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003234
Physical Location & Seq
Reverse (-)
3857344 .. 3864533
7190 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1034700.v1.1.491

Sequence Viewer

Length: 3057 bp
ATGAGCAGAAGGCGGTTGAAAACTCTCCTCTGGCAATCAATTTCCACCGTATCTTCGTCACCTCTGAAGTCCAACCCCTTTTTCTCCACAAACCCATCACACCCATTCCCTTCTCCATTCAAATCCCACCAAAGTCTAAAACTTTGCTTCCCCAGATTGCTTTGCACTTCGTCGGAGGACAATCTCGATGGTCTGGTCGATCCCAACGACTTGTGCAAGCCGGAGAGTTCCGGAGTTGAGTCTATTTCGGAAGAGGAATTAGCCTCTCTGCGCGATTCGGTGTTGGATACCAGTGCTGGTGGTGGGTCTTCGATGCCAAAATTTGAATCAGCTAAGTTTTCTAACAATGCCATTTTGATTTCGAATGCTATGAGGAATAATATTGATGAGTTTGATGACAAAACCCAGAAGTTTCTTAGGCAGTTTAGGGATAATCTGAATGAAACTCTGGTGGTGGAGGTGTTGAAGCTTATACAAAATCCTGCATTGGGTGTGAAATTTTTCATATGGGCAGGTAGACAAATTGGGTATAGTCACACTGCTTCCGTGTACAACACATTGTTAGAGTTATTGGAGTGTGGTAATAAGAGGGTAGCAGATCATTTTCTGCAAGAAATTAAGGGTGATGATAGAGAGGTGCTGTGGAAGTTGCTTAATGTGTTAATTCGGAATTACTGCCGAAATGGGTTGTGGAATGTAGCTTTGGAAGAGCTGGGGAGGCTTAAGGATTTCGGATACAAGCCAACTCCAGCAACATACAATGCTTTAGTTCAAGTGTTTCTAGAAGTAGATAAGTTGGACACAGCACACTTGGTCCATGTGGAGATGTTGGATTCAGGGTTTAAGATGGATGAGTATACATTTGGTTCATTTGTTCACGCCCTTTGCAAAGCTGGGAGGTGGAAGGAAGCTCTCACATTGGTTGAGAAGGAAGAGTTTGTGCCCAATACTATTCTTTACACGAAGATGATATCTGGGTTGTGTGAAGCTTCACTTTTTGAGGAAGCTATGGATTTTTTGAACAGAATGCGATGTGATTCTTGCATTCCTAATGTTATGACCTATAGGACTTTGCTTTGTGGGTGTTTGAAGAAAAAACAGCTCGGTAGGTGCAAGAGAATTCTTAGTATGATGATCACAGAAGGTTGTTATCCAAGCCCTCAGATATTTAGTTCGCTTGTACATGCTTATTGCAGGTCCGGAGAGTACTCTTATGCTTATAAGTTGCTTAAGAAAATGGTAAGATGTGAGTGCCAGCCAGGCTACGTGGTTTACAATATCTTGATTGGTGGTATATGTGGAAACAGGGAGTCACCAAGCCCAGATATGTTAGATTTGGCTGAAAAAGCTTATGGTGAGATGCTTGATGCAGGGGTTGTGCTAAGTAAGGTCAATGTGAGCAATTTTGCATGGTGTCTTTGCGGTGCCGGAAAATTTGAGAAAGCCTATAAGGTTATCAGCGAAATGATGAGCAAGGGTTTTGTTCCTGACACTAGTACATATGCGAATGTTATTGGCTTTTTGTGTAATGCCTCCAAGGTAGAACAAGCATTTTTGTTGTTTAAAGAAATGAAAAGGAATAGTATTGTTCCTGATGTCTATACCTATACTATTTTAATTGATAGTTTTTGTAAAGCTGGCCTTATTGAACAATCTCGGAGCTGGTTTAATGAAATGATACGAAATGGTTGTGCTCCAAACGTGGTGACATATACTGCTCTTATACATGCATACCTTAAGGCAAAGAAGGTGTCTGATGCTGATCAACTCTTTGAGATGATGTTGACTGAATGTTGCAGTCCTAATGTTATTACTTATACTGCTTTAATTGATGGTCATTTTAAAGCTGGTAGAATTGAAAAGGCTTGCCAGATATATGAGAGAATGAGAGGTAATATGGACGTCCCTGATGTTGATAAGTATTTTGGAAGTGATGATCAGAGTATTAGCAAACCTAATGTCTATACTTATGGGGCTTTGGTGGATGGTTTATGCAAAGCACACAAGGTTAAAGAGGCCCGTGATTTATTGCATGCTATGTCAGAAGAAGGTTGTGAACCTAACCATATTATTTATGATGCTCTTATAGATGGATTTTGCAAGTATGGGAAGCTAGATGAAGCACAAGAGGTGTTTGCTAAGATGTCAGAGCAAGGGTACAGTCCAAATGTGTATACGTACAGTTCTTTGATTGATAGGTTGTTTAAGGATAAAAGACTGGATCTCGCTTTAAAAGTCTTGTCCAAAATGTTAGAAAACTCTTGTGCACCAAATGTTGTTATCTACACAGAGATGATTGATGCCCTGTGTAAAGTTGGAAAAACAGATGAAGCCTATAAACTTATGCTGATGATGGAAGAAAAAGGGTGTTATCCAAATGTTGTGACTTATACTGCAATGATATATGGCTTTGGGAAAGCAGGTAATATCGAAAAGTGTCTTGAGCTTTTTAAACAAATGAGCTCTAAGGGTTGTGCTCCAAATTTTATTACCTATGGGGTTTTGATAAATCATTGCTGTTCAACTGGACTACTGGATGAAGCTCGTAAGCTTTTGGATGAAATGAAACAAACGCATTGGCCTAAGTATATGGCAGGCTACCGTAAGGTTATTGAAGGTTACAATAGAGAGTTCATGACCTCTCTTGGGCTTTTAAACGAGATGAGTGAATGCGACACAGTGCCAATTACTCACATATACAAAGTTCTGATTGATAATTTTGTAAAGGCTGGAAGGTTGGAAATGGCTCTTGAGCTGCTTGAAGAGATTTCATCATCTTCACCGTTTACATGTGTGAACAAGAATATGTACACTTCATTGATTGAGAGTCTTTCACATGCAAATAAGGTCAGTAAGGCACTGGAGATGTTTGGAGACATGATGAGGCATGGTGGTTTTCCAGAGCTGGATACATTCTTTCATCTTATTAAAGGGCTTATTAAAGCTAACAAGTGGGATGAAGCTCTTCAACTATCAGATAGCATATGCCACATGGAGGCAACCCAGTGTCCGGATATCCATTGGCATATGCAAGAAGAGACATCTGACAGAAATTAG

Protein Analysis

1019

Amino Acids

115.09

Weight (kDa)

6.01

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 250 - 297 9.2e-08 PPR repeat family
PPR_1 PF12854 280 - 310 2.7e-06 PPR repeat
PPR_1 PF12854 313 - 344 5.9e-06 PPR repeat
PPR_2 PF13041 315 - 364 8.3e-12 PPR repeat family
PPR_2 PF13041 389 - 433 9.5e-08 PPR repeat family
PPR_long PF17177 425 - 547 7.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 472 - 510 8.1e-07 PPR repeat family
PPR_3 PF13812 485 - 543 6.1e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 496 - 545 5.6e-17 PPR repeat family
PPR_long PF17177 517 - 631 2.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 529 - 559 3.3e-09 PPR repeat
PPR PF01535 534 - 564 3.3e-08 PPR repeat
PPR_2 PF13041 538 - 578 5e-11 PPR repeat family
PPR_3 PF13812 556 - 610 1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 562 - 594 4.4e-07 PPR repeat
PPR_2 PF13041 566 - 614 1.9e-15 PPR repeat family
PPR PF01535 569 - 598 9.2e-06 PPR repeat
PPR_1 PF12854 598 - 630 2.6e-10 PPR repeat
PPR_2 PF13041 601 - 637 1.9e-07 PPR repeat family
PPR PF01535 604 - 631 3.6e-06 PPR repeat
PPR_3 PF13812 651 - 697 3.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 651 - 680 1.7e-08 PPR repeat
PPR_2 PF13041 652 - 689 2e-08 PPR repeat family
PPR_1 PF12854 684 - 716 1.2e-11 PPR repeat
PPR_2 PF13041 687 - 736 6.6e-15 PPR repeat family
TPR_24 PF23276 689 - 780 2.6e-06 Fungal tetratrico peptide repeats
PPR PF01535 691 - 719 3.7e-06 PPR repeat
PPR_3 PF13812 691 - 732 8.1e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 711 - 768 5.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 719 - 750 8.1e-08 PPR repeat
PPR_long PF17177 739 - 858 1.7e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 754 - 785 4.5e-08 PPR repeat
PPR_2 PF13041 757 - 806 5e-16 PPR repeat family
PPR_3 PF13812 757 - 806 7.3e-08 Pentatricopeptide repeat domain
PPR PF01535 760 - 790 2.6e-06 PPR repeat
PPR_3 PF13812 785 - 839 5e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 788 - 821 1.5e-09 PPR repeat
PPR_2 PF13041 792 - 840 1.6e-16 PPR repeat family
PPR PF01535 795 - 825 2.9e-09 PPR repeat
PPR_1 PF12854 823 - 856 3e-10 PPR repeat
PPR PF01535 830 - 857 6.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1221
AatII GACGTC 1 cut(s) 1905
Acc36I ACCTGC 3 cut(s) 503, 1185, 2411
AccB1I GGYRCC 1 cut(s) 1424
AccI GTMKAC 3 cut(s) 517, 857, 2174
AccII CGCG 1 cut(s) 273
AccIII TCCGGA 3 cut(s) 230, 1199, 3010
AciI CCGC 2 cut(s) 13, 1422
AclWI GGATC 2 cut(s) 194, 2229
AcsI RAATTY 5 cut(s) 320, 497, 1119, 1433, 2482
AcuI CTGAAG 1 cut(s) 86
AcyI GRCGYC 1 cut(s) 1902
AfaI GTAC 7 cut(s) 551, 1182, 1208, 1498, 2159, 2180, 2810
AfiI CCNNNNNNNGG 4 cut(s) 488, 2646, 2995, 3010
AflII CTTAAG 3 cut(s) 722, 1229, 1736
AflIII ACRYGT 1 cut(s) 2789
AhlI ACTAGT 1 cut(s) 1493
AjnI CCWGG 1 cut(s) 1258
AjuI GAANNNNNNNTTGG 4 cut(s) 65, 97, 686, 718
Alw21I GWGCWC 4 cut(s) 1696, 2269, 2465, 2479
Alw26I GTCTC 2 cut(s) 2868, 3032
Alw44I GTGCAC 1 cut(s) 2265
AlwI GGATC 2 cut(s) 194, 2229
Aor13HI TCCGGA 3 cut(s) 230, 1199, 3010
AoxI GGCC 3 cut(s) 1639, 2016, 2579
ApaLI GTGCAC 1 cut(s) 2265
ApeKI GCWGC 1 cut(s) 2755
ApoI RAATTY 5 cut(s) 320, 497, 1119, 1433, 2482
ArsI GACNNNNNNTTYG 4 cut(s) 1736, 1768, 1944, 1976
Asp700I GAANNNNTTC 2 cut(s) 500, 2964
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 3 cut(s) 814, 1197, 2017
AsuHPI GGTGA 6 cut(s) 51, 635, 1305, 1367, 1717, 2772
AsuII TTCGAA 1 cut(s) 362
AvaII GGWCC 2 cut(s) 814, 1197
BaeGI GKGCMC 2 cut(s) 945, 2269
BanI GGYRCC 1 cut(s) 1424
BanII GRGCYC 1 cut(s) 2465
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 4 cut(s) 1696, 2269, 2465, 2479
BbvI GCAGC 1 cut(s) 2742
BccI CCATC 7 cut(s) 103, 182, 841, 1826, 1979, 2084, 2347
BciT130I CCWGG 1 cut(s) 1260
BciVI GTATCC 3 cut(s) 280, 728, 2902
BclI TGATCA 3 cut(s) 1134, 1762, 1936
BcoDI GTCTC 2 cut(s) 2868, 3032
BcuI ACTAGT 1 cut(s) 1493
BfaI CTAG 3 cut(s) 782, 1494, 2114
BfmI CTRYAG 1 cut(s) 1063
BfrI CTTAAG 3 cut(s) 722, 1229, 1736
BfuAI ACCTGC 3 cut(s) 503, 1185, 2411
BfuI GTATCC 3 cut(s) 280, 728, 2902
BglI GCCNNNNNGGC 1 cut(s) 1260
BisI GCNGC 1 cut(s) 2756
BlsI GCNGC 1 cut(s) 2757
BmcAI AGTACT 1 cut(s) 1208
Bme1390I CCNGG 1 cut(s) 1260
Bme18I GGWCC 2 cut(s) 814, 1197
BmgT120I GGNCC 3 cut(s) 814, 1197, 2017
BmiI GGNNCC 1 cut(s) 1426
BmrFI CCNGG 1 cut(s) 1260
BmrI ACTGGG 1 cut(s) 2998
BmsI GCATC 6 cut(s) 303, 1350, 1357, 1747, 2068, 2290
BmuI ACTGGG 1 cut(s) 2998
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 2 cut(s) 732, 2882
Bpu14I TTCGAA 1 cut(s) 362
BpuEI CTTGAG 2 cut(s) 2462, 2771
BsaAI YACGTR 2 cut(s) 1267, 2178
BsaBI GATNNNNATC 1 cut(s) 1761
BsaHI GRCGYC 1 cut(s) 1902
BsaJI CCNNGG 1 cut(s) 1536
BsaWI WCCGGW 3 cut(s) 230, 1199, 3010
Bsc4I CCNNNNNNNGG 4 cut(s) 488, 2646, 2995, 3010
Bse1I ACTGG 6 cut(s) 291, 2223, 2530, 2538, 2865, 3004
Bse3DI GCAATG 2 cut(s) 2403, 2512
Bse8I GATNNNNATC 1 cut(s) 1761
BseAI TCCGGA 3 cut(s) 230, 1199, 3010
BseBI CCWGG 1 cut(s) 1260
BseDI CCNNGG 1 cut(s) 1536
BseGI GGATG 5 cut(s) 856, 1990, 2542, 2563, 2962
BseJI GATNNNNATC 1 cut(s) 1761
BseLI CCNNNNNNNGG 4 cut(s) 488, 2646, 2995, 3010
BseMI GCAATG 2 cut(s) 2403, 2512
BseMII CTCAG 1 cut(s) 1175
BseNI ACTGG 6 cut(s) 291, 2223, 2530, 2538, 2865, 3004
BseRI GAGGAG 1 cut(s) 17
BseSI GKGCMC 2 cut(s) 945, 2269
BseXI GCAGC 1 cut(s) 2742
BseYI CCCAGC 2 cut(s) 712, 893
Bsh1236I CGCG 1 cut(s) 273
BshFI GGCC 3 cut(s) 1641, 2018, 2581
BshNI GGYRCC 1 cut(s) 1424
BsiHKAI GWGCWC 4 cut(s) 1696, 2269, 2465, 2479
BsiSI CCGG 5 cut(s) 221, 231, 1200, 1428, 3011
BslFI GGGAC 1 cut(s) 1889
BslI CCNNNNNNNGG 4 cut(s) 488, 2646, 2995, 3010
BsmAI GTCTC 2 cut(s) 2868, 3032
BsmFI GGGAC 1 cut(s) 1889
BsmI GAATGC 4 cut(s) 370, 1032, 1044, 2675
BsnI GGCC 3 cut(s) 1641, 2018, 2581
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 5 cut(s) 945, 1696, 2269, 2465, 2479
Bsp13I TCCGGA 3 cut(s) 230, 1199, 3010
Bsp1407I TGTACA 3 cut(s) 549, 1180, 2808
Bsp143I GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
BspACI CCGC 2 cut(s) 13, 1422
BspANI GGCC 3 cut(s) 1641, 2018, 2581
BspCNI CTCAG 1 cut(s) 1174
BspEI TCCGGA 3 cut(s) 230, 1199, 3010
BspFNI CGCG 1 cut(s) 273
BspHI TCATGA 1 cut(s) 2634
BspLI GGNNCC 1 cut(s) 1426
BspMI ACCTGC 3 cut(s) 503, 1185, 2411
BspPI GGATC 2 cut(s) 194, 2229
BspQI GCTCTTC 2 cut(s) 702, 2970
BspT104I TTCGAA 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 1424
BspTI CTTAAG 3 cut(s) 722, 1229, 1736
BsrDI GCAATG 2 cut(s) 2403, 2512
BsrGI TGTACA 3 cut(s) 549, 1180, 2808
BsrI ACTGG 6 cut(s) 291, 2223, 2530, 2538, 2865, 3004
BssECI CCNNGG 1 cut(s) 1536
BssMI GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
BssNAI GTATAC 2 cut(s) 858, 2175
BssNI GRCGYC 1 cut(s) 1902
BssT1I CCWWGG 1 cut(s) 1536
Bst1107I GTATAC 2 cut(s) 858, 2175
Bst2UI CCWGG 1 cut(s) 1260
Bst4CI ACNGT 6 cut(s) 49, 2162, 2183, 2603, 2680, 2784
Bst6I CTCTTC 6 cut(s) 246, 702, 927, 2757, 2970, 3030
BstACI GRCGYC 1 cut(s) 1902
BstAFI CTTAAG 3 cut(s) 722, 1229, 1736
BstAUI TGTACA 3 cut(s) 549, 1180, 2808
BstBAI YACGTR 2 cut(s) 1267, 2178
BstBI TTCGAA 1 cut(s) 362
BstC8I GCNNGC 6 cut(s) 218, 1256, 1639, 1867, 2034, 2596
BstDEI CTNAG 8 cut(s) 333, 416, 1124, 1161, 1382, 2139, 2466, 2583
BstF5I GGATG 5 cut(s) 856, 1990, 2542, 2563, 2962
BstFNI CGCG 1 cut(s) 273
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 6 cut(s) 202, 601, 1137, 1765, 1939, 2224
BstMAI GTCTC 2 cut(s) 2868, 3032
BstMBI GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
BstMWI GCNNNNNNNGC 3 cut(s) 718, 1260, 1346
BstNI CCWGG 1 cut(s) 1260
BstNSI RCATGY 5 cut(s) 1187, 1730, 2036, 2793, 2840
BstSCI CCNGG 1 cut(s) 1258
BstSFI CTRYAG 1 cut(s) 1063
BstSLI GKGCMC 2 cut(s) 945, 2269
BstSNI TACGTA 1 cut(s) 2178
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 2742
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 2221
BstYI RGATCY 1 cut(s) 2221
BstZ17I GTATAC 2 cut(s) 858, 2175
BsuI GTATCC 3 cut(s) 280, 728, 2902
BsuRI GGCC 3 cut(s) 1641, 2018, 2581
BtgZI GCGATG 1 cut(s) 1045
BtsCI GGATG 5 cut(s) 856, 1990, 2542, 2563, 2962
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 5 cut(s) 298, 537, 2685, 2858, 3011
BveI ACCTGC 3 cut(s) 503, 1185, 2411
Cac8I GCNNGC 6 cut(s) 218, 1256, 1639, 1867, 2034, 2596
CciI TCATGA 1 cut(s) 2634
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 3 cut(s) 814, 1197, 2017
Csp6I GTAC 7 cut(s) 550, 1181, 1207, 1497, 2158, 2179, 2809
CviQI GTAC 7 cut(s) 550, 1181, 1207, 1497, 2158, 2179, 2809
DdeI CTNAG 8 cut(s) 333, 416, 1124, 1161, 1382, 2139, 2466, 2583
DpnI GATC 6 cut(s) 201, 600, 1136, 1764, 1938, 2223
DpnII GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
DraI TTTAAA 5 cut(s) 1564, 1843, 2232, 2452, 2655
Eam1104I CTCTTC 6 cut(s) 246, 702, 927, 2757, 2970, 3030
EarI CTCTTC 6 cut(s) 246, 702, 927, 2757, 2970, 3030
Ecl136II GAGCTC 1 cut(s) 2463
Eco105I TACGTA 1 cut(s) 2178
Eco130I CCWWGG 1 cut(s) 1536
Eco24I GRGCYC 1 cut(s) 2465
Eco32I GATATC 2 cut(s) 972, 3016
Eco47I GGWCC 2 cut(s) 814, 1197
Eco53kI GAGCTC 1 cut(s) 2463
Eco57I CTGAAG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 2463
EcoRI GAATTC 1 cut(s) 1119
EcoRII CCWGG 1 cut(s) 1258
EcoRV GATATC 2 cut(s) 972, 3016
EcoT14I CCWWGG 1 cut(s) 1536
EcoT22I ATGCAT 1 cut(s) 1732
EcoT38I GRGCYC 1 cut(s) 2465
ErhI CCWWGG 1 cut(s) 1536
FalI AAGNNNNNCTT 4 cut(s) 978, 1010, 1626, 1658
FaqI GGGAC 1 cut(s) 1889
FauNDI CATATG 4 cut(s) 506, 1501, 2984, 3027
FbaI TGATCA 3 cut(s) 1134, 1762, 1936
FblI GTMKAC 3 cut(s) 517, 857, 2174
Fnu4HI GCNGC 1 cut(s) 2756
FokI GGATG 5 cut(s) 863, 1997, 2549, 2570, 2969
FriOI GRGCYC 1 cut(s) 2465
Fsp4HI GCNGC 1 cut(s) 2756
FspBI CTAG 3 cut(s) 782, 1494, 2114
GlaI GCGC 1 cut(s) 272
GluI GCNGC 1 cut(s) 2756
GsaI CCCAGC 2 cut(s) 716, 897
GsuI CTGGAG 2 cut(s) 732, 2882
HaeIII GGCC 3 cut(s) 1641, 2018, 2581
HapII CCGG 5 cut(s) 221, 231, 1200, 1428, 3011
HhaI GCGC 1 cut(s) 273
Hin1I GRCGYC 1 cut(s) 1902
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HincII GTYRAC 1 cut(s) 1785
HindII GTYRAC 1 cut(s) 1785
HindIII AAGCTT 4 cut(s) 467, 987, 1347, 2549
HinfI GANTC 7 cut(s) 239, 275, 326, 833, 1037, 1310, 2827
HpaII CCGG 5 cut(s) 221, 231, 1200, 1428, 3011
HphI GGTGA 6 cut(s) 51, 635, 1305, 1367, 1717, 2772
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 9 cut(s) 3, 120, 898, 922, 1136, 1741, 2042, 2609, 2727
HpyCH4III ACNGT 6 cut(s) 49, 2162, 2183, 2603, 2680, 2784
HpyCH4IV ACGT 4 cut(s) 1266, 1701, 1902, 2177
HpyF10VI GCNNNNNNNGC 3 cut(s) 718, 1260, 1346
HpyF3I CTNAG 8 cut(s) 333, 416, 1124, 1161, 1382, 2139, 2466, 2583
HpySE526I ACGT 4 cut(s) 1266, 1701, 1902, 2177
Hsp92I GRCGYC 1 cut(s) 1902
HspAI GCGC 1 cut(s) 271
Kpn2I TCCGGA 3 cut(s) 230, 1199, 3010
Ksp22I TGATCA 3 cut(s) 1134, 1762, 1936
Kzo9I GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
LguI GCTCTTC 2 cut(s) 702, 2970
LmnI GCTCC 3 cut(s) 1659, 1699, 2482
Lsp1109I GCAGC 1 cut(s) 2742
LweI GCATC 6 cut(s) 303, 1350, 1357, 1747, 2068, 2290
MaeI CTAG 3 cut(s) 782, 1494, 2114
MaeII ACGT 4 cut(s) 1266, 1701, 1902, 2177
MaeIII GTNAC 6 cut(s) 57, 533, 1311, 1705, 2383, 2618
MalI GATC 6 cut(s) 201, 600, 1136, 1764, 1938, 2223
MboI GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
MflI RGATCY 1 cut(s) 2221
MhlI GDGCHC 5 cut(s) 945, 1696, 2269, 2465, 2479
MlyI GAGTC 3 cut(s) 248, 1319, 2836
MmeI TCCRAC 7 cut(s) 96, 153, 264, 777, 810, 2296, 2718
Mph1103I ATGCAT 1 cut(s) 1732
MroI TCCGGA 3 cut(s) 230, 1199, 3010
MroXI GAANNNNTTC 2 cut(s) 500, 2964
MslI CAYNNNNRTG 2 cut(s) 965, 2291
MspCI CTTAAG 3 cut(s) 722, 1229, 1736
MspI CCGG 5 cut(s) 221, 231, 1200, 1428, 3011
MspR9I CCNGG 1 cut(s) 1260
Mva1269I GAATGC 4 cut(s) 370, 1032, 1044, 2675
MvaI CCWGG 1 cut(s) 1260
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 3 cut(s) 718, 1260, 1346
NdeI CATATG 4 cut(s) 506, 1501, 2984, 3027
NdeII GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
NlaIV GGNNCC 1 cut(s) 1426
NmuCI GTSAC 5 cut(s) 57, 533, 1311, 1705, 2383
NsiI ATGCAT 1 cut(s) 1732
NspI RCATGY 5 cut(s) 1187, 1730, 2036, 2793, 2840
NspV TTCGAA 1 cut(s) 362
PaeI GCATGC 1 cut(s) 2036
PagI TCATGA 1 cut(s) 2634
PciI ACATGT 1 cut(s) 2789
PciSI GCTCTTC 2 cut(s) 702, 2970
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 4 cut(s) 370, 1032, 1044, 2675
PdmI GAANNNNTTC 2 cut(s) 500, 2964
PfeI GAWTC 4 cut(s) 275, 326, 833, 1037
PkrI GCNGC 1 cut(s) 2757
PleI GAGTC 3 cut(s) 247, 1318, 2835
PpsI GAGTC 3 cut(s) 247, 1318, 2835
Ppu21I YACGTR 2 cut(s) 1267, 2178
PscI ACATGT 1 cut(s) 2789
PsiI TTATAA 1 cut(s) 1221
Psp124BI GAGCTC 1 cut(s) 2465
Psp6I CCWGG 1 cut(s) 1258
PspFI CCCAGC 2 cut(s) 712, 893
PspGI CCWGG 1 cut(s) 1258
PspN4I GGNNCC 1 cut(s) 1426
PspPI GGNCC 3 cut(s) 814, 1197, 2017
PsuI RGATCY 1 cut(s) 2221
RsaI GTAC 7 cut(s) 551, 1182, 1208, 1498, 2159, 2180, 2810
RsaNI GTAC 7 cut(s) 550, 1181, 1207, 1497, 2158, 2179, 2809
RseI CAYNNNNRTG 2 cut(s) 965, 2291
SacI GAGCTC 1 cut(s) 2465
SapI GCTCTTC 2 cut(s) 702, 2970
SatI GCNGC 1 cut(s) 2756
Sau3AI GATC 6 cut(s) 199, 598, 1134, 1762, 1936, 2221
Sau96I GGNCC 3 cut(s) 814, 1197, 2017
ScaI AGTACT 1 cut(s) 1208
SchI GAGTC 3 cut(s) 248, 1319, 2836
ScrFI CCNGG 1 cut(s) 1260
SduI GDGCHC 5 cut(s) 945, 1696, 2269, 2465, 2479
SfaNI GCATC 6 cut(s) 303, 1350, 1357, 1747, 2068, 2290
SfcI CTRYAG 1 cut(s) 1063
SfuI TTCGAA 1 cut(s) 362
SinI GGWCC 2 cut(s) 814, 1197
SmiMI CAYNNNNRTG 2 cut(s) 965, 2291
SmlI CTYRAG 5 cut(s) 722, 1229, 1736, 2441, 2750
SmoI CTYRAG 5 cut(s) 722, 1229, 1736, 2441, 2750
SnaBI TACGTA 1 cut(s) 2178
SpeI ACTAGT 1 cut(s) 1493
SphI GCATGC 1 cut(s) 2036
SsiI CCGC 2 cut(s) 13, 1422
SspI AATATT 1 cut(s) 382
SspMI CTAG 3 cut(s) 782, 1494, 2114
SstI GAGCTC 1 cut(s) 2465
StyD4I CCNGG 1 cut(s) 1258
StyI CCWWGG 1 cut(s) 1536
TaaI ACNGT 6 cut(s) 49, 2162, 2183, 2603, 2680, 2784
TaiI ACGT 4 cut(s) 1269, 1704, 1905, 2180
TaqI TCGA 5 cut(s) 186, 198, 311, 362, 2430
TatI WGTACW 5 cut(s) 549, 1180, 1206, 1496, 2808
TfiI GAWTC 4 cut(s) 275, 326, 833, 1037
TscAI CASTG 5 cut(s) 298, 544, 2685, 2865, 3011
TseFI GTSAC 5 cut(s) 57, 533, 1311, 1705, 2383
TseI GCWGC 1 cut(s) 2755
Tsp45I GTSAC 5 cut(s) 57, 533, 1311, 1705, 2383
TspGWI ACGGA 1 cut(s) 535
TspRI CASTG 5 cut(s) 298, 544, 2685, 2865, 3011
Vha464I CTTAAG 3 cut(s) 722, 1229, 1736
VneI GTGCAC 1 cut(s) 2265
VpaK11BI GGWCC 2 cut(s) 814, 1197
XapI RAATTY 5 cut(s) 320, 497, 1119, 1433, 2482
XbaI TCTAGA 1 cut(s) 781
XceI RCATGY 5 cut(s) 1187, 1730, 2036, 2793, 2840
XmiI GTMKAC 3 cut(s) 517, 857, 2174
XmnI GAANNNNTTC 2 cut(s) 500, 2964
XspI CTAG 3 cut(s) 782, 1494, 2114
ZraI GACGTC 1 cut(s) 1903
ZrmI AGTACT 1 cut(s) 1208
Zsp2I ATGCAT 1 cut(s) 1732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.