RchiOBHm_Chr4g0408411

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003234
Physical Location & Seq
Reverse (-)
31093526 .. 31099313
5788 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37954

Sequence Viewer

Length: 2946 bp
ATGAGCAGAAGACACTTCAGCTCTATACTCTTCAACTCCATCTCTTCTTCTCCCCTTTTCTCTAGAAACTCATCCCAATCCTTCGTTTCTTCACTCAAATCATACCCACAATGTCTCTTCTCCAGATTCCTCTGCACCTCTCCGCCGGACAATCTCGACGGCCTCCTCGATCCCCAGGACTTCTCTACGCCGGAGACCCACTCTCTTTCCGCACAAGACTTCGCCTTTTTGCGCCACTCGCCGAAACTTGACCCCGATAAGTGTTCTAACGACGCCGTTTTGATTTCTAATGCCATTAGGAATAGTTCTGATTCATTTGGGGATGAAACCCACAAGTTTCTTAGGAGGTTTAGGGATAATCTGAATGAAACTTTGGTGGTGCAGGTGTTGAGTCTTGTAAAGAGTCCTGAGTTGGGTGTCAAGTTTTTCATTTGGGCAGGTAGGCAAATTGGGTATAGTCATACTGCTTCTGTATATAATGCATTGTTGGAGTTACTGGAGTGTGGTAGTAGCGACAGAGTACCGGAGCATTTTTTGCGAGAAATTATGGGTGATGATAGAGAGGTGCTTGGGAAGTTGCTTAATGTGTTGATTCGCAAGTGTTGCCGAAATGGTTTGTGGAATGTAGCTTTGGAAGAGCTTGGGAGGCTTAAGGATTTCGGGTACAGGCCGAGTCAAGCAACTTACAATGCATTGGTTCAAGTGTTTTTGAGAGCTGATAGGCTGGACACAGCGCACTTGGTTCATGGGGAGATGTTGGAGTTGGGGTTTAAGATGGATGAGTATACGCTAGGTTGTTTTGGTCATGCCCTGTGTAAAGCTGGGAGGTGGAAGGAGGGACTTGCATTGATTGAGAAGGAAGAGTTTGTTCCCGATACTGTTCTTTATACCAAGATGATTTCTGGGTTGTGTGAGGCTTCACTTTTTGAGGAAGCTATGGATTTGTTGAGCAGAATGCGCTGTAATTCTTGCATTCCTAATGTTGTAACCTATAGGATTTTGCTTTGTGGATGTTTGAAGAAAAAACAGCTTGGCAGGTGCAAGAGAATTCTTAGTATGATGATCACGGAAGGTTGTTATCCTAGCCCTGGGATATTTAATTCTCTTGTTCATGCTTATTGCAGATCAGGAGATTACTCTTATGCTTATAAGTTGCTAAAGAAAATGGTCAGGTGTGGGTGCTGGCCAGGCTATGTGGTTTACAATATCTTGATTGGTAGTATCTGTGGACACGAAGAATTACCTACCTTAGATATGCTAGACATGGCTGAAAAAGCTTATGGTGAGATGCTTAATGCAGGGGTTGTTTTAAATAAGGTAAATGTAAGCAATTTTGTGCGATGTCTTTGTGGCCATGGAAAATTTGACAAAGCCTACAAGGTCATCAATGAAATGATGAGCAAGGGTTTTGTTCCTGACACTAGTACATACTCTAAAGTTATTGGCTTTTTGTGCAATGCCTCCAAGGTAGAACAAGCATTATTGTTGTTTGAAGAAATGAAGCAAAACGGTGTTGTTCCTGATGTCTACACCTATACCATCTTAATTGATAGTTTTTCTAAGGCTGGCCTTATTGAACAGGCTCGGAACTGGTTTAATGAAATGGTAGGAAATGGTTGTGCACCAAACGTGGTAACTTATACTGCTCTTATACATGCATACCTTAAAGCAAGGAAGGTGCCTGATGCAAATCAACTCTTTGAGATGATGTTGACTCAAGGTTGCATTCCTAATGCTATTACTTATAGTGCTTTGATCGATGGCCATTGTAAAGCTGGAGAGACTGAAAAGGCTTGCCTGATTTATGCAAGAATGAGAGGTAATGTGGAAGTCCCTGATGTTGATATGTATTTCAAAATTGATGATCAGAGTTTGAAAGAACCTAATGTTCATACTTATGGAGCGTTGGTGGATGGTTTATGTAAAGCCCACAAGGTCAGAGAGGCCGGTGACTTATTGGATGCTATGTTGGTAGAAGGTTGTGAACCTAACCACATTGTTTATGATGCTCTTATAGATGGATTTTGCAAGTCTGGGAAGCTCGATGAAGCACAAAAGGTGTTTGCTAAAATGTCAGAGCATGGGTATAGTCCAAATGTGTATACGTACAGCTCTTTGATTGACAGGTTGTTCAAGGATAAAAGACTGGATCTTGTTTTAAAAGTCCTGTCTAAAATGTTAGAAAACTCTTGTTCACCAAATGTTGTTATCTATACAGAGATGGTCGATGGCCTTTGTAAGGTTGGAAAGACAGATGAAGCTTATAAGCTTATGCTAATGATGGAAGAAAAAGGATGTAATCCAAATGTTGTGACTTACACTGCGATGATAGATGGCCTTGGGAAAGCAGGCAAAATTGACAAATGTCTTGAGCTCTTTAAAGCAATGAGCTCCAAGGGTTGTGCTCCCAATTTTATCACCTACAAGGTTTTGATAAACCATTGCTGTGCAATTGGTCTTTTGGATGAAGCTCATAAACTTTTGGATGAAATGAAACAAACATATTGGCCAAAGCATTTGGCAGGCTACCGTAAAGTTATTGAAGGTTACAACCGAGAATTCATAGCCTCACTTGGGCTTTTAAATGAGATAAGTGAGTGCGACTCATTGCCAATCGTTCACATATACAGAGTTTTGGTTGATAATTTTGTAAAGGCTGGAAGACTGGAAGTGGCTCTGGAGCTGCATGAAGAGATTTCTTCATCTACTCCTTTTACATCTGTGAACAAGGATATGTATACTTTACTGATCGAGAACCTATCACATGCAAATAAGGCTGATAAGGCACTGCAGATGTTTGCAGAAATGATAAGGCTGGGTGGTTATCCAGAGCTGAGTACATTTTTCCACCTCATCAAAGGGCTTATTAAAATTAATAGGTGGGATGAAGCTCTTCAACTATCGGATAGCATATGCCAGATGGATATCCAATGGGCTTCTGCGTGA

Protein Analysis

981

Amino Acids

110.2

Weight (kDa)

6.3

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 224 - 273 3.8e-06 PPR repeat family
PPR_1 PF12854 289 - 320 2.5e-06 PPR repeat
PPR_2 PF13041 291 - 340 4.5e-14 PPR repeat family
PPR_1 PF12854 358 - 390 1.6e-06 PPR repeat
PPR PF01535 365 - 394 3.7e-07 PPR repeat
PPR_2 PF13041 365 - 409 3.1e-08 PPR repeat family
PPR_long PF17177 401 - 523 1.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 445 - 486 7.2e-09 PPR repeat family
PPR_3 PF13812 460 - 519 4.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 472 - 521 6.9e-16 PPR repeat family
PPR_long PF17177 490 - 608 1.1e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 503 - 536 5.8e-09 PPR repeat
PPR PF01535 510 - 540 2.4e-07 PPR repeat
PPR_2 PF13041 515 - 554 2.3e-10 PPR repeat family
PPR_3 PF13812 532 - 587 1.3e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 538 - 570 4.4e-07 PPR repeat
PPR_2 PF13041 542 - 591 5e-17 PPR repeat family
PPR PF01535 545 - 575 8e-06 PPR repeat
PPR_1 PF12854 574 - 605 7.4e-09 PPR repeat
PPR_1 PF12854 627 - 656 7.8e-07 PPR repeat
PPR_2 PF13041 628 - 668 8.3e-08 PPR repeat family
PPR_3 PF13812 652 - 708 3.9e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 660 - 692 1.2e-12 PPR repeat
PPR_2 PF13041 665 - 712 6.2e-15 PPR repeat family
PPR PF01535 667 - 695 4.6e-07 PPR repeat
PPR_3 PF13812 686 - 739 1.1e-08 Pentatricopeptide repeat domain
PPR_long PF17177 714 - 836 2.8e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 722 - 781 3.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 730 - 761 1.4e-08 PPR repeat
PPR_2 PF13041 733 - 782 1.2e-16 PPR repeat family
PPR_3 PF13812 760 - 815 1.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 764 - 796 1.5e-10 PPR repeat
PPR_2 PF13041 768 - 816 5.9e-17 PPR repeat family
PPR PF01535 771 - 801 3e-09 PPR repeat
PPR_1 PF12854 799 - 832 1.1e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1149, 2265
AarI CACCTGC 2 cut(s) 373, 1026
Acc36I ACCTGC 3 cut(s) 373, 428, 1026
AccB1I GGYRCC 1 cut(s) 1678
AccI GTMKAC 4 cut(s) 785, 1527, 2102, 2738
AciI CCGC 2 cut(s) 143, 210
AclWI GGATC 2 cut(s) 164, 2157
AcoI YGGCCR 4 cut(s) 1184, 1351, 1762, 2507
AcsI RAATTY 3 cut(s) 1047, 1361, 2558
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 5 cut(s) 522, 665, 1426, 2108, 2839
AfiI CCNNNNNNNGG 4 cut(s) 413, 1088, 2239, 2574
AflII CTTAAG 1 cut(s) 650
AhlI ACTAGT 1 cut(s) 1421
AjnI CCWGG 3 cut(s) 174, 1087, 1186
AjuI GAANNNNNNNTTGG 4 cut(s) 356, 388, 614, 646
Alw21I GWGCWC 4 cut(s) 1624, 2376, 2393, 2407
Alw26I GTCTC 3 cut(s) 119, 188, 1775
Alw44I GTGCAC 1 cut(s) 1620
AlwI GGATC 2 cut(s) 164, 2157
ApaLI GTGCAC 1 cut(s) 1620
ApeKI GCWGC 1 cut(s) 2683
ApoI RAATTY 3 cut(s) 1047, 1361, 2558
AseI ATTAAT 1 cut(s) 2874
Asp700I GAANNNNTTC 1 cut(s) 2892
AspLEI GCGC 3 cut(s) 234, 736, 960
AsuHPI GGTGA 5 cut(s) 563, 1295, 1961, 2187, 2410
BaeGI GKGCMC 1 cut(s) 1624
BalI TGGCCA 4 cut(s) 1186, 1353, 1764, 2509
BanI GGYRCC 1 cut(s) 1678
BanII GRGCYC 2 cut(s) 2376, 2393
BbsI GAAGAC 2 cut(s) 16, 2668
Bbv12I GWGCWC 4 cut(s) 1624, 2376, 2393, 2407
BbvI GCAGC 1 cut(s) 2670
BceAI ACGGC 2 cut(s) 175, 260
BciT130I CCWGG 3 cut(s) 176, 1089, 1188
BclI TGATCA 2 cut(s) 1062, 1864
BcoDI GTCTC 3 cut(s) 119, 188, 1775
BcuI ACTAGT 1 cut(s) 1421
BfaI CTAG 5 cut(s) 63, 791, 1083, 1259, 1422
BfmI CTRYAG 2 cut(s) 991, 2789
BfrI CTTAAG 1 cut(s) 650
BfuAI ACCTGC 3 cut(s) 373, 428, 1026
BisI GCNGC 1 cut(s) 2684
BlsI GCNGC 1 cut(s) 2685
Bme1390I CCNGG 3 cut(s) 176, 1089, 1188
BmiI GGNNCC 1 cut(s) 1680
BmrFI CCNGG 3 cut(s) 176, 1089, 1188
BmsI GCATC 4 cut(s) 1278, 1675, 1951, 1996
BoxI GACNNNNGTC 1 cut(s) 2364
BpiI GAAGAC 2 cut(s) 16, 2668
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 2588, 2620
BpmI CTGGAG 4 cut(s) 106, 518, 1797, 2699
BpuEI CTTGAG 2 cut(s) 1701, 2390
Bsa29I ATCGAT 1 cut(s) 1758
BsaAI YACGTR 1 cut(s) 2106
BsaBI GATNNNNATC 2 cut(s) 1689, 2924
BsaHI GRCGYC 1 cut(s) 273
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 7 cut(s) 174, 1087, 1088, 1354, 1464, 2338, 2394
BsaWI WCCGGW 1 cut(s) 523
BsaXI ACNNNNNCTCC 4 cut(s) 752, 782, 1893, 1923
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 1088, 2239, 2574
Bse118I RCCGGY 1 cut(s) 1946
Bse1I ACTGG 4 cut(s) 501, 1595, 2151, 2670
Bse3DI GCAATG 4 cut(s) 1462, 2391, 2440, 2606
Bse8I GATNNNNATC 2 cut(s) 1689, 2924
BseBI CCWGG 3 cut(s) 176, 1089, 1188
BseCI ATCGAT 1 cut(s) 1758
BseDI CCNNGG 7 cut(s) 174, 1087, 1088, 1354, 1464, 2338, 2394
BseJI GATNNNNATC 2 cut(s) 1689, 2924
BseLI CCNNNNNNNGG 4 cut(s) 413, 1088, 2239, 2574
BseMI GCAATG 4 cut(s) 1462, 2391, 2440, 2606
BseMII CTCAG 2 cut(s) 399, 2825
BseNI ACTGG 4 cut(s) 501, 1595, 2151, 2670
BseRI GAGGAG 1 cut(s) 155
BseSI GKGCMC 1 cut(s) 1624
BseXI GCAGC 1 cut(s) 2670
BseYI CCCAGC 2 cut(s) 821, 2815
BsgI GTGCAG 2 cut(s) 118, 401
BshNI GGYRCC 1 cut(s) 1678
BshVI ATCGAT 1 cut(s) 1758
BsiHKAI GWGCWC 4 cut(s) 1624, 2376, 2393, 2407
BsiSI CCGG 4 cut(s) 146, 191, 524, 1947
BslFI GGGAC 2 cut(s) 852, 1817
BslI CCNNNNNNNGG 4 cut(s) 413, 1088, 2239, 2574
BsmAI GTCTC 3 cut(s) 119, 188, 1775
BsmFI GGGAC 2 cut(s) 852, 1817
BsmI GAATGC 3 cut(s) 960, 972, 1725
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 4 cut(s) 1624, 2376, 2393, 2407
Bsp143I GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
Bsp19I CCATGG 1 cut(s) 1354
BspACI CCGC 2 cut(s) 143, 210
BspCNI CTCAG 2 cut(s) 400, 2826
BspDI ATCGAT 1 cut(s) 1758
BspLI GGNNCC 1 cut(s) 1680
BspMAI CTGCAG 1 cut(s) 2793
BspMI ACCTGC 3 cut(s) 373, 428, 1026
BspPI GGATC 2 cut(s) 164, 2157
BspQI GCTCTTC 2 cut(s) 630, 2898
BspT107I GGYRCC 1 cut(s) 1678
BspTI CTTAAG 1 cut(s) 650
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 4 cut(s) 1462, 2391, 2440, 2606
BsrFI RCCGGY 1 cut(s) 1946
BsrI ACTGG 4 cut(s) 501, 1595, 2151, 2670
BssAI RCCGGY 1 cut(s) 1946
BssECI CCNNGG 7 cut(s) 174, 1087, 1088, 1354, 1464, 2338, 2394
BssMI GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
BssNAI GTATAC 3 cut(s) 786, 2103, 2739
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 4 cut(s) 1354, 1464, 2338, 2394
Bst1107I GTATAC 3 cut(s) 786, 2103, 2739
Bst2UI CCWGG 3 cut(s) 176, 1089, 1188
Bst4CI ACNGT 3 cut(s) 880, 1511, 2531
Bst6I CTCTTC 7 cut(s) 35, 49, 122, 630, 855, 2685, 2898
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 650
BstAPI GCANNNNNTGC 2 cut(s) 535, 603
BstBAI YACGTR 1 cut(s) 2106
BstC8I GCNNGC 5 cut(s) 1184, 1567, 1795, 2350, 2524
BstDEI CTNAG 6 cut(s) 341, 408, 1052, 1249, 1560, 2834
BstDSI CCRYGG 1 cut(s) 1354
BstENI CCTNNNNNAGG 1 cut(s) 2237
BstHHI GCGC 3 cut(s) 234, 736, 960
BstKTI GATC 7 cut(s) 172, 1065, 1127, 1758, 1867, 2152, 2751
BstMAI GTCTC 3 cut(s) 119, 188, 1775
BstMBI GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
BstNI CCWGG 3 cut(s) 176, 1089, 1188
BstNSI RCATGY 2 cut(s) 1658, 2768
BstPAI GACNNNNGTC 1 cut(s) 2364
BstSCI CCNGG 3 cut(s) 174, 1087, 1186
BstSFI CTRYAG 2 cut(s) 991, 2789
BstSLI GKGCMC 1 cut(s) 1624
BstSNI TACGTA 1 cut(s) 2106
BstV1I GCAGC 1 cut(s) 2670
BstV2I GAAGAC 2 cut(s) 16, 2668
BstX2I RGATCY 1 cut(s) 2149
BstYI RGATCY 1 cut(s) 2149
BstZ17I GTATAC 3 cut(s) 786, 2103, 2739
Bsu15I ATCGAT 1 cut(s) 1758
BsuTUI ATCGAT 1 cut(s) 1758
BtgI CCRYGG 1 cut(s) 1354
BtgZI GCGATG 2 cut(s) 1354, 2339
BtsI GCAGTG 2 cut(s) 2319, 2786
BtsIMutI CAGTG 2 cut(s) 2319, 2786
BveI ACCTGC 3 cut(s) 373, 428, 1026
Cac8I GCNNGC 5 cut(s) 1184, 1567, 1795, 2350, 2524
CfoI GCGC 3 cut(s) 234, 736, 960
Cfr10I RCCGGY 1 cut(s) 1946
ClaI ATCGAT 1 cut(s) 1758
CseI GACGC 1 cut(s) 281
Csp6I GTAC 5 cut(s) 521, 664, 1425, 2107, 2838
CviAII CATG 9 cut(s) 746, 806, 1112, 1264, 1355, 1655, 2081, 2687, 2765
CviQI GTAC 5 cut(s) 521, 664, 1425, 2107, 2838
DdeI CTNAG 6 cut(s) 341, 408, 1052, 1249, 1560, 2834
DpnI GATC 7 cut(s) 171, 1064, 1126, 1757, 1866, 2151, 2750
DpnII GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
DraI TTTAAA 4 cut(s) 1311, 2160, 2380, 2583
EaeI YGGCCR 4 cut(s) 1184, 1351, 1762, 2507
Eam1104I CTCTTC 7 cut(s) 35, 49, 122, 630, 855, 2685, 2898
EarI CTCTTC 7 cut(s) 35, 49, 122, 630, 855, 2685, 2898
EciI GGCGGA 1 cut(s) 132
Ecl136II GAGCTC 2 cut(s) 2374, 2391
Eco105I TACGTA 1 cut(s) 2106
Eco130I CCWWGG 4 cut(s) 1354, 1464, 2338, 2394
Eco24I GRGCYC 2 cut(s) 2376, 2393
Eco31I GGTCTC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 2926
Eco53kI GAGCTC 2 cut(s) 2374, 2391
EcoICRI GAGCTC 2 cut(s) 2374, 2391
EcoNI CCTNNNNNAGG 1 cut(s) 2237
EcoRI GAATTC 2 cut(s) 1047, 2558
EcoRII CCWGG 3 cut(s) 174, 1087, 1186
EcoRV GATATC 1 cut(s) 2926
EcoT14I CCWWGG 4 cut(s) 1354, 1464, 2338, 2394
EcoT22I ATGCAT 3 cut(s) 484, 694, 1660
EcoT38I GRGCYC 2 cut(s) 2376, 2393
ErhI CCWWGG 4 cut(s) 1354, 1464, 2338, 2394
FaeI CATG 9 cut(s) 749, 809, 1115, 1267, 1358, 1658, 2084, 2690, 2768
FaqI GGGAC 2 cut(s) 852, 1817
FatI CATG 9 cut(s) 745, 805, 1111, 1263, 1354, 1654, 2080, 2686, 2764
FauNDI CATATG 1 cut(s) 2912
FbaI TGATCA 2 cut(s) 1062, 1864
FblI GTMKAC 4 cut(s) 785, 1527, 2102, 2738
Fnu4HI GCNGC 1 cut(s) 2684
FriOI GRGCYC 2 cut(s) 2376, 2393
Fsp4HI GCNGC 1 cut(s) 2684
FspBI CTAG 5 cut(s) 63, 791, 1083, 1259, 1422
GlaI GCGC 3 cut(s) 233, 735, 959
GluI GCNGC 1 cut(s) 2684
GsaI CCCAGC 2 cut(s) 825, 2819
GsuI CTGGAG 4 cut(s) 106, 518, 1797, 2699
HapII CCGG 4 cut(s) 146, 191, 524, 1947
HgaI GACGC 1 cut(s) 281
HhaI GCGC 3 cut(s) 234, 736, 960
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 9 cut(s) 749, 809, 1115, 1267, 1358, 1658, 2084, 2690, 2768
Hin6I GCGC 3 cut(s) 232, 734, 958
HinP1I GCGC 3 cut(s) 232, 734, 958
HincII GTYRAC 1 cut(s) 1713
HindII GTYRAC 1 cut(s) 1713
HindIII AAGCTT 3 cut(s) 1275, 2259, 2267
HinfI GANTC 8 cut(s) 126, 311, 391, 403, 592, 673, 1714, 2603
HpaII CCGG 4 cut(s) 146, 191, 524, 1947
HphI GGTGA 5 cut(s) 563, 1295, 1961, 2187, 2410
Hpy188I TCNGA 7 cut(s) 310, 363, 1587, 1869, 1940, 2077, 2905
Hpy99I CGWCG 2 cut(s) 161, 275
HpyAV CCTTC 7 cut(s) 91, 826, 850, 1064, 1669, 1970, 2537
HpyCH4III ACNGT 3 cut(s) 880, 1511, 2531
HpyCH4IV ACGT 2 cut(s) 1629, 2105
HpyF3I CTNAG 6 cut(s) 341, 408, 1052, 1249, 1560, 2834
HpySE526I ACGT 2 cut(s) 1629, 2105
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 9 cut(s) 749, 809, 1115, 1267, 1358, 1658, 2084, 2690, 2768
HspAI GCGC 3 cut(s) 232, 734, 958
Ksp22I TGATCA 2 cut(s) 1062, 1864
Kzo9I GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
LguI GCTCTTC 2 cut(s) 630, 2898
LmnI GCTCC 5 cut(s) 526, 1901, 2396, 2410, 2680
Lsp1109I GCAGC 1 cut(s) 2670
LweI GCATC 4 cut(s) 1278, 1675, 1951, 1996
MaeI CTAG 5 cut(s) 63, 791, 1083, 1259, 1422
MaeII ACGT 2 cut(s) 1629, 2105
MaeIII GTNAC 6 cut(s) 492, 985, 1633, 1949, 2311, 2546
MalI GATC 7 cut(s) 171, 1064, 1126, 1757, 1866, 2151, 2750
MboI GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
MfeI CAATTG 1 cut(s) 2451
MflI RGATCY 1 cut(s) 2149
MhlI GDGCHC 4 cut(s) 1624, 2376, 2393, 2407
MlsI TGGCCA 4 cut(s) 1186, 1353, 1764, 2509
MluNI TGGCCA 4 cut(s) 1186, 1353, 1764, 2509
MlyI GAGTC 5 cut(s) 400, 412, 682, 1708, 2597
MmeI TCCRAC 3 cut(s) 468, 738, 2224
Mox20I TGGCCA 4 cut(s) 1186, 1353, 1764, 2509
Mph1103I ATGCAT 3 cut(s) 484, 694, 1660
MroXI GAANNNNTTC 1 cut(s) 2892
MscI TGGCCA 4 cut(s) 1186, 1353, 1764, 2509
MslI CAYNNNNRTG 3 cut(s) 1896, 2324, 2445
Msp20I TGGCCA 4 cut(s) 1186, 1353, 1764, 2509
MspCI CTTAAG 1 cut(s) 650
MspI CCGG 4 cut(s) 146, 191, 524, 1947
MspR9I CCNGG 3 cut(s) 176, 1089, 1188
MunI CAATTG 1 cut(s) 2451
Mva1269I GAATGC 3 cut(s) 960, 972, 1725
MvaI CCWGG 3 cut(s) 176, 1089, 1188
NcoI CCATGG 1 cut(s) 1354
NdeI CATATG 1 cut(s) 2912
NdeII GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
NlaIII CATG 9 cut(s) 749, 809, 1115, 1267, 1358, 1658, 2084, 2690, 2768
NlaIV GGNNCC 1 cut(s) 1680
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 2 cut(s) 1949, 2311
NsiI ATGCAT 3 cut(s) 484, 694, 1660
NspI RCATGY 2 cut(s) 1658, 2768
PaqCI CACCTGC 2 cut(s) 373, 1026
PasI CCCWGGG 1 cut(s) 1088
PciSI GCTCTTC 2 cut(s) 630, 2898
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 3 cut(s) 960, 972, 1725
PdmI GAANNNNTTC 1 cut(s) 2892
PfeI GAWTC 3 cut(s) 126, 311, 592
PkrI GCNGC 1 cut(s) 2685
PleI GAGTC 5 cut(s) 399, 411, 681, 1708, 2597
PpsI GAGTC 5 cut(s) 399, 411, 681, 1708, 2597
Ppu21I YACGTR 1 cut(s) 2106
PshAI GACNNNNGTC 1 cut(s) 2364
PshBI ATTAAT 1 cut(s) 2874
PsiI TTATAA 2 cut(s) 1149, 2265
Psp124BI GAGCTC 2 cut(s) 2376, 2393
Psp6I CCWGG 3 cut(s) 174, 1087, 1186
PspFI CCCAGC 2 cut(s) 821, 2815
PspGI CCWGG 3 cut(s) 174, 1087, 1186
PspN4I GGNNCC 1 cut(s) 1680
PstI CTGCAG 1 cut(s) 2793
PsuI RGATCY 1 cut(s) 2149
RsaI GTAC 5 cut(s) 522, 665, 1426, 2108, 2839
RsaNI GTAC 5 cut(s) 521, 664, 1425, 2107, 2838
RseI CAYNNNNRTG 3 cut(s) 1896, 2324, 2445
SacI GAGCTC 2 cut(s) 2376, 2393
SapI GCTCTTC 2 cut(s) 630, 2898
SatI GCNGC 1 cut(s) 2684
Sau3AI GATC 7 cut(s) 169, 1062, 1124, 1755, 1864, 2149, 2748
SchI GAGTC 5 cut(s) 400, 412, 682, 1708, 2597
ScrFI CCNGG 3 cut(s) 176, 1089, 1188
SduI GDGCHC 4 cut(s) 1624, 2376, 2393, 2407
SfaNI GCATC 4 cut(s) 1278, 1675, 1951, 1996
SfcI CTRYAG 2 cut(s) 991, 2789
SmiMI CAYNNNNRTG 3 cut(s) 1896, 2324, 2445
SmlI CTYRAG 3 cut(s) 650, 1716, 2369
SmoI CTYRAG 3 cut(s) 650, 1716, 2369
SnaBI TACGTA 1 cut(s) 2106
SpeI ACTAGT 1 cut(s) 1421
SsiI CCGC 2 cut(s) 143, 210
SspMI CTAG 5 cut(s) 63, 791, 1083, 1259, 1422
SstI GAGCTC 2 cut(s) 2376, 2393
StyD4I CCNGG 3 cut(s) 174, 1087, 1186
StyI CCWWGG 4 cut(s) 1354, 1464, 2338, 2394
TaaI ACNGT 3 cut(s) 880, 1511, 2531
TaiI ACGT 2 cut(s) 1632, 2108
TaqI TCGA 6 cut(s) 156, 168, 1758, 2043, 2226, 2751
TatI WGTACW 2 cut(s) 1424, 2837
TfiI GAWTC 3 cut(s) 126, 311, 592
TscAI CASTG 2 cut(s) 2326, 2793
TseFI GTSAC 2 cut(s) 1949, 2311
TseI GCWGC 1 cut(s) 2683
Tsp45I GTSAC 2 cut(s) 1949, 2311
TspGWI ACGGA 1 cut(s) 1082
TspRI CASTG 2 cut(s) 2326, 2793
Vha464I CTTAAG 1 cut(s) 650
VneI GTGCAC 1 cut(s) 1620
VspI ATTAAT 1 cut(s) 2874
XagI CCTNNNNNAGG 1 cut(s) 2237
XapI RAATTY 3 cut(s) 1047, 1361, 2558
XbaI TCTAGA 1 cut(s) 62
XceI RCATGY 2 cut(s) 1658, 2768
XcmI CCANNNNNNNNNTGG 1 cut(s) 1772
XmiI GTMKAC 4 cut(s) 785, 1527, 2102, 2738
XmnI GAANNNNTTC 1 cut(s) 2892
XspI CTAG 5 cut(s) 63, 791, 1083, 1259, 1422
Zsp2I ATGCAT 3 cut(s) 484, 694, 1660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.