FvH4_4g12620

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006322
Physical Location & Seq
Reverse (-)
16285402 .. 16288549
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12620.t1

Sequence Viewer

Length: 909 bp
ATGGCTCGTAACGAAGAGAAAGCGCAGTCGATGCTGAATCGCTTCATCACCCTGAAGAACGAAGAGAAGAAGAAGCCCCGCGAGCGCCGCCCTTTCCTCGCCTCCGAATGCCGCGACCTCGCCGAGGCCTCCAAGTGGCGCCAGCAGATCATGGGAGAGATCGGCCGCAAAGTCGCCGAGATCCAAAACGAAGGCCTCGGCGAGCACCGCCTCCGCGACCTCAACGACGAGATCAACAAGCTCATAAGGGAGAAGTCTCACTGGGAGCGCCGCATCGTCGAGCTCGGCGGCCCTAATTACGCCAAGCACGCCCCGAAGATGACCGATCTCGACGGCAACATCGTCGACGTTCCAAACCCTAGCGGCCGCGGCGCTGGTTATCGGTACTTTGGTGCGGCGAAGAAGCTTCCCGGTGTGAGGGAGCTTTTCGAGAAGCCGCCGGAGCTGAGGAAGCGGCGGACGAGGTATGATATATACAAGAGGATTGATGCGAGCTATTATGGATATAGGGACGATGAGGATGGGGTTTTGGAGAAGCTGGAGGTTGTGGCGGAGGAGAAGATGAGGGCCGAGGCGGTGGCGGAGTGGTGGAGGTTGGAGGAGATTAGGAGAGAGGCCAAGAGAGCGGTGAAGAGTGGCGAAGTGGTGACGGTGGCGAAGGCTAAGGAGATTCTGGTGGAGGAGGAGGAGGAGGTGGTGGAGGAAGAGAGGCAGAGAGAGAGGGAGTTGTTGAAAGAGAAGAGTGAGAAAGAGATGGAGTTTGTGGCTCACGTGCCGCTGCCCGACGATAAGGAGATTGAGAAGATGGTGCTTGAGAAGAAGAAGAAGGACTTGCTGAGTAGGTATGTCAGTGAGGAACTGCTGCAGGGCCAGACTGAGGCTAAGCAGATGCTTAACATTAAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

303

Amino Acids

35.56

Weight (kDa)

7.71

Isoelectric Point (pI)

57.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 1 - 281 1.5e-91 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 138
AccBSI CCGCTC 1 cut(s) 626
AccI GTMKAC 1 cut(s) 345
AccII CGCG 4 cut(s) 81, 114, 216, 369
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 163, 364
AcuI CTGAAG 1 cut(s) 74
AcvI CACGTG 1 cut(s) 772
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 4 cut(s) 124, 135, 417, 877
AgsI TTSAA 1 cut(s) 733
AluBI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
AluI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
Alw21I GWGCWC 2 cut(s) 207, 285
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 1 cut(s) 175
AoxI GGCC 8 cut(s) 126, 163, 193, 289, 364, 567, 615, 868
ApeKI GCWGC 2 cut(s) 778, 862
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 5 cut(s) 25, 87, 141, 270, 374
AspS9I GGNCC 3 cut(s) 290, 567, 868
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 40, 640, 658
BaeI ACNNNNGTAYC 2 cut(s) 376, 409
BanI GGYRCC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 772
Bbv12I GWGCWC 2 cut(s) 207, 285
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 765, 849
BccI CCATC 3 cut(s) 515, 748, 799
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 863
BfoI RGCGCY 4 cut(s) 88, 142, 271, 375
BlpI GCTNAGC 1 cut(s) 882
Bme1390I CCNGG 1 cut(s) 411
BmgT120I GGNCC 3 cut(s) 290, 567, 868
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 4 cut(s) 21, 282, 478, 879
BmuI ACTGGG 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 560
Bpu10I CCTNAGC 2 cut(s) 446, 663
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 772
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 4 cut(s) 123, 196, 367, 570
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 135, 417, 877
Bse1I ACTGG 1 cut(s) 266
BseDI CCNNGG 4 cut(s) 123, 196, 367, 570
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 4 cut(s) 124, 135, 417, 877
BseMII CTCAG 3 cut(s) 437, 827, 867
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 6 cut(s) 569, 614, 695, 698, 701, 704
BseX3I CGGCCG 2 cut(s) 163, 364
BseXI GCAGC 2 cut(s) 765, 849
Bsh1236I CGCG 4 cut(s) 81, 114, 216, 369
Bsh1285I CGRYCG 2 cut(s) 166, 367
BshFI GGCC 8 cut(s) 128, 165, 195, 291, 366, 569, 617, 870
BshNI GGYRCC 1 cut(s) 138
BsiEI CGRYCG 2 cut(s) 166, 367
BsiHKAI GWGCWC 2 cut(s) 207, 285
BsiSI CCGG 2 cut(s) 411, 440
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 124, 135, 417, 877
BsmAI GTCTC 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 8 cut(s) 128, 165, 195, 291, 366, 569, 617, 870
Bsp1286I GDGCHC 2 cut(s) 207, 285
Bsp143I GATC 5 cut(s) 147, 159, 180, 231, 325
Bsp1720I GCTNAGC 1 cut(s) 882
BspANI GGCC 8 cut(s) 128, 165, 195, 291, 366, 569, 617, 870
BspCNI CTCAG 3 cut(s) 438, 828, 868
BspFNI CGCG 4 cut(s) 81, 114, 216, 369
BspLI GGNNCC 1 cut(s) 140
BspMAI CTGCAG 1 cut(s) 867
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 138
BsrBI CCGCTC 1 cut(s) 626
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 4 cut(s) 123, 196, 367, 570
BssMI GATC 5 cut(s) 147, 159, 180, 231, 325
BssNI GRCGYC 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 5 cut(s) 9, 57, 626, 699, 734
BstACI GRCGYC 1 cut(s) 139
BstAPI GCANNNNNTGC 1 cut(s) 31
BstBAI YACGTR 1 cut(s) 772
BstC8I GCNNGC 5 cut(s) 83, 143, 203, 309, 493
BstDEI CTNAG 5 cut(s) 446, 663, 836, 876, 882
BstDSI CCRYGG 1 cut(s) 367
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 4 cut(s) 81, 114, 216, 369
BstH2I RGCGCY 4 cut(s) 88, 142, 271, 375
BstHHI GCGC 5 cut(s) 25, 87, 141, 270, 374
BstKTI GATC 5 cut(s) 150, 162, 183, 234, 328
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 5 cut(s) 147, 159, 180, 231, 325
BstMCI CGRYCG 2 cut(s) 166, 367
BstMWI GCNNNNNNNGC 9 cut(s) 31, 82, 87, 207, 308, 369, 442, 451, 623
BstSCI CCNGG 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 863
BstUI CGCG 4 cut(s) 81, 114, 216, 369
BstV1I GCAGC 2 cut(s) 765, 849
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 2 cut(s) 163, 364
BsuRI GGCC 8 cut(s) 128, 165, 195, 291, 366, 569, 617, 870
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 2 cut(s) 259, 856
Cac8I GCNNGC 5 cut(s) 83, 143, 203, 309, 493
CciNI GCGGCCGC 1 cut(s) 364
CfoI GCGC 5 cut(s) 25, 87, 141, 270, 374
Cfr13I GGNCC 3 cut(s) 290, 567, 868
Cfr42I CCGCGG 1 cut(s) 370
Csp6I GTAC 1 cut(s) 385
CviAII CATG 1 cut(s) 151
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 5 cut(s) 446, 663, 836, 876, 882
DinI GGCGCC 1 cut(s) 140
DpnI GATC 5 cut(s) 149, 161, 182, 233, 327
DpnII GATC 5 cut(s) 147, 159, 180, 231, 325
EaeI YGGCCR 2 cut(s) 163, 364
EagI CGGCCG 2 cut(s) 163, 364
Eam1104I CTCTTC 5 cut(s) 9, 57, 626, 699, 734
EarI CTCTTC 5 cut(s) 9, 57, 626, 699, 734
EciI GGCGGA 3 cut(s) 472, 566, 596
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 2 cut(s) 163, 364
Eco147I AGGCCT 2 cut(s) 128, 195
Eco24I GRGCYC 1 cut(s) 285
Eco52I CGGCCG 2 cut(s) 163, 364
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 74
Eco72I CACGTG 1 cut(s) 772
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 1 cut(s) 140
EheI GGCGCC 1 cut(s) 140
FaeI CATG 1 cut(s) 154
FaiI YATR 8 cut(s) 152, 245, 468, 473, 475, 501, 507, 846
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 1 cut(s) 524
FatI CATG 1 cut(s) 150
FauI CCCGC 1 cut(s) 86
FblI GTMKAC 1 cut(s) 345
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 1 cut(s) 360
GlaI GCGC 5 cut(s) 24, 86, 140, 269, 373
GsuI CTGGAG 1 cut(s) 560
HaeII RGCGCY 4 cut(s) 88, 142, 271, 375
HaeIII GGCC 8 cut(s) 128, 165, 195, 291, 366, 569, 617, 870
HapII CCGG 2 cut(s) 411, 440
HhaI GCGC 5 cut(s) 25, 87, 141, 270, 374
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 1 cut(s) 154
Hin6I GCGC 5 cut(s) 23, 85, 139, 268, 372
HinP1I GCGC 5 cut(s) 23, 85, 139, 268, 372
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 404
HinfI GANTC 2 cut(s) 37, 670
HpaII CCGG 2 cut(s) 411, 440
HphI GGTGA 3 cut(s) 40, 640, 658
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 2 cut(s) 329, 430
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 6 cut(s) 230, 281, 335, 347, 350, 788
HpyAV CCTTC 3 cut(s) 185, 652, 820
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 2 cut(s) 348, 771
HpyCH4V TGCA 1 cut(s) 865
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 82, 87, 207, 308, 369, 442, 451, 623
HpyF3I CTNAG 5 cut(s) 446, 663, 836, 876, 882
HpySE526I ACGT 2 cut(s) 348, 771
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 1 cut(s) 154
HspAI GCGC 5 cut(s) 23, 85, 139, 268, 372
KasI GGCGCC 1 cut(s) 138
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 5 cut(s) 147, 159, 180, 231, 325
LmnI GCTCC 3 cut(s) 265, 421, 442
Lsp1109I GCAGC 2 cut(s) 765, 849
LweI GCATC 4 cut(s) 21, 282, 478, 879
MaeI CTAG 1 cut(s) 360
MaeII ACGT 2 cut(s) 348, 771
MaeIII GTNAC 2 cut(s) 8, 646
MalI GATC 5 cut(s) 149, 161, 182, 233, 327
MbiI CCGCTC 1 cut(s) 626
MboI GATC 5 cut(s) 147, 159, 180, 231, 325
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 207, 285
MluCI AATT 1 cut(s) 295
Mly113I GGCGCC 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 2 cut(s) 894, 900
MspA1I CMGCKG 2 cut(s) 369, 778
MspI CCGG 2 cut(s) 411, 440
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 113
MvnI CGCG 4 cut(s) 81, 114, 216, 369
MwoI GCNNNNNNNGC 9 cut(s) 31, 82, 87, 207, 308, 369, 442, 451, 623
NarI GGCGCC 1 cut(s) 139
NciI CCSGG 1 cut(s) 411
NdeII GATC 5 cut(s) 147, 159, 180, 231, 325
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 1 cut(s) 140
NmeAIII GCCGAG 5 cut(s) 148, 177, 202, 264, 595
NmuCI GTSAC 1 cut(s) 646
NotI GCGGCCGC 1 cut(s) 364
PceI AGGCCT 2 cut(s) 128, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 113
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 2 cut(s) 37, 670
PluTI GGCGCC 1 cut(s) 142
PmaCI CACGTG 1 cut(s) 772
PmlI CACGTG 1 cut(s) 772
Ppu21I YACGTR 1 cut(s) 772
Psp124BI GAGCTC 1 cut(s) 285
PspCI CACGTG 1 cut(s) 772
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 3 cut(s) 290, 567, 868
PstI CTGCAG 1 cut(s) 867
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 1 cut(s) 370
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 2 cut(s) 894, 900
Sau3AI GATC 5 cut(s) 147, 159, 180, 231, 325
Sau96I GGNCC 3 cut(s) 290, 567, 868
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 2 cut(s) 207, 285
SfaNI GCATC 4 cut(s) 21, 282, 478, 879
SfcI CTRYAG 1 cut(s) 863
SfoI GGCGCC 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SgrDI CGTCGACG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 1 cut(s) 295
SseBI AGGCCT 2 cut(s) 128, 195
SspDI GGCGCC 1 cut(s) 138
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 2 cut(s) 128, 195
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 2 cut(s) 351, 774
TaqI TCGA 5 cut(s) 29, 279, 330, 345, 429
TaqII GACCGA 1 cut(s) 338
TasI AATT 1 cut(s) 295
TfiI GAWTC 2 cut(s) 37, 670
Tru1I TTAA 2 cut(s) 894, 900
Tru9I TTAA 2 cut(s) 894, 900
TscAI CASTG 2 cut(s) 266, 856
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 2 cut(s) 778, 862
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 34
TspRI CASTG 2 cut(s) 266, 856
XagI CCTNNNNNAGG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.