MD12G1216200.v1.1

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006322
Physical Location & Seq
Forward (+)
29399709 .. 29400614
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1216200.v1.1.491

Sequence Viewer

Length: 906 bp
ATGGCTCGTAACGAAGAGAAAGCGCAGTCGATGCTCAATCGATTCATTACCCTCAAAGCCGAAGAGAAGAAGAAGCCAAAAGAGCGACGCCCATACCTCGCCTCCGAGTGCCGCGATCTCGCCGAGGCCTCCAGATGGCGGCAACAAATCATGGGGGAGATCGGCCGGAAAGTTGCCGAGATCCAGAACGAAGCCCTCGGGGAGCACCGGACCCGCGACCTCAACGACGAGATCAACAAGCTAATTCGAGAGAAGGTCCATTGGGAGCGGCGGATTGTGGAGCTCGGAGGCCCAAATTACGCCAAAAACGCCCCTAAGATGACCGACCTCGACGGCAATATTGTCGATGTTCCAAACCCCAGCGGCCGCGGCCCCGGGTATCGGTACTTTGGAGCGGCGAAGAAGCTGCCCGGAGTCAGGGAGCTTTTCGAGAAGCCTCCGGAGCTGAGAAAGCGGAGGACGAGGTATGATATATACAAGAGGATTGATGCTAGTTATTATGGGTATAGGGACGATGAGGATGGGATTTTGGAGAAGCTTGAGGTAGAGGCGGAGGAGAAGATGAGGGCGGAGGCGGTGGCGGAGTGGAAGAGGATGGAGGAGATAAGGCGTGAGGCGAGGAGGGCGGTGAAGAGCGGGGAGGTGGTGTCGGCTGCAAAGGTGAAGGAGGTTTTGTTTGAGGAGGAAGAGGAGGTGGTGGAGGAGGAGAGGCAGAGGGAGAGGGAATTGAAAGAGAGGAGTGAGCAGGAGAGGGAGTTTGTGGTGCACGTGCCGCTGCCGGATGATAAGGAGATTGAGAAGATGGTGCTGGAGAAGAAGAAGAGGGAGCTGCTGAGTAAGTACACCAGTGATGAATTGATGGAGGAGCAGACCGAGGCTAAAGATATGCTTAACATTAAGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

302

Amino Acids

35.65

Weight (kDa)

6.2

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 1 - 280 2e-91 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 268, 395, 636
AccII CGCG 3 cut(s) 114, 216, 369
AccIII TCCGGA 1 cut(s) 439
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 163, 364
AcvI CACGTG 1 cut(s) 769
AcyI GRCGYC 1 cut(s) 88
AfaI GTAC 2 cut(s) 386, 842
AfeI AGCGCT 1 cut(s) 902
AfiI CCNNNNNNNGG 4 cut(s) 135, 138, 381, 417
AgsI TTSAA 1 cut(s) 730
AluBI AGCT 7 cut(s) 241, 283, 406, 424, 445, 538, 829
AluI AGCT 7 cut(s) 241, 283, 406, 424, 445, 538, 829
Alw21I GWGCWC 3 cut(s) 207, 285, 768
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 2 cut(s) 197, 374
Aor13HI TCCGGA 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 902
AoxI GGCC 5 cut(s) 126, 163, 289, 364, 370
ApaLI GTGCAC 1 cut(s) 764
ApeKI GCWGC 4 cut(s) 406, 653, 775, 829
AspLEI GCGC 2 cut(s) 25, 903
AspS9I GGNCC 4 cut(s) 210, 256, 290, 371
AsuC2I CCSGG 3 cut(s) 375, 376, 411
AsuHPI GGTGA 2 cut(s) 640, 673
AvaI CYCGRG 2 cut(s) 197, 374
AvaII GGWCC 2 cut(s) 210, 256
BaeGI GKGCMC 1 cut(s) 768
BanII GRGCYC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 769
Bbv12I GWGCWC 3 cut(s) 207, 285, 768
BbvI GCAGC 4 cut(s) 393, 640, 762, 816
BccI CCATC 5 cut(s) 129, 515, 589, 796, 853
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 4 cut(s) 325, 359, 388, 422
BcnI CCSGG 3 cut(s) 375, 376, 411
BfaI CTAG 2 cut(s) 492, 904
BfoI RGCGCY 1 cut(s) 904
Bme1390I CCNGG 3 cut(s) 375, 376, 411
Bme18I GGWCC 2 cut(s) 210, 256
BmeT110I CYCGRG 2 cut(s) 197, 374
BmgT120I GGNCC 4 cut(s) 210, 256, 290, 371
BmiI GGNNCC 2 cut(s) 212, 373
BmrFI CCNGG 3 cut(s) 375, 376, 411
BmsI GCATC 2 cut(s) 21, 478
BpmI CTGGAG 2 cut(s) 115, 830
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 3 cut(s) 375, 376, 411
Bsa29I ATCGAT 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 769
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 6 cut(s) 123, 196, 367, 373, 374, 873
BsaWI WCCGGW 2 cut(s) 207, 439
BsaXI ACNNNNNCTCC 8 cut(s) 86, 116, 632, 659, 662, 689, 740, 770
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 138, 381, 417
Bse1I ACTGG 1 cut(s) 846
BseAI TCCGGA 1 cut(s) 439
BseCI ATCGAT 1 cut(s) 40
BseDI CCNNGG 6 cut(s) 123, 196, 367, 373, 374, 873
BseGI GGATG 3 cut(s) 526, 600, 787
BseLI CCNNNNNNNGG 4 cut(s) 135, 138, 381, 417
BseMII CTCAG 2 cut(s) 437, 824
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 9 cut(s) 569, 614, 634, 695, 704, 716, 719, 751, 878
BseSI GKGCMC 1 cut(s) 768
BseX3I CGGCCG 2 cut(s) 163, 364
BseXI GCAGC 4 cut(s) 393, 640, 762, 816
BseYI CCCAGC 1 cut(s) 359
Bsh1236I CGCG 3 cut(s) 114, 216, 369
Bsh1285I CGRYCG 2 cut(s) 166, 367
BshFI GGCC 5 cut(s) 128, 165, 291, 366, 372
BshVI ATCGAT 1 cut(s) 40
BsiEI CGRYCG 2 cut(s) 166, 367
BsiHKAI GWGCWC 3 cut(s) 207, 285, 768
BsiHKCI CYCGRG 2 cut(s) 197, 374
BsiSI CCGG 6 cut(s) 166, 208, 375, 411, 440, 779
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 135, 138, 381, 417
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 5 cut(s) 128, 165, 291, 366, 372
BsoBI CYCGRG 2 cut(s) 197, 374
Bsp1286I GDGCHC 3 cut(s) 207, 285, 768
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 4 cut(s) 115, 159, 180, 231
BspANI GGCC 5 cut(s) 128, 165, 291, 366, 372
BspCNI CTCAG 2 cut(s) 438, 825
BspDI ATCGAT 1 cut(s) 40
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 3 cut(s) 114, 216, 369
BspLI GGNNCC 2 cut(s) 212, 373
BspPI GGATC 1 cut(s) 175
BspQI GCTCTTC 1 cut(s) 626
BsrBI CCGCTC 3 cut(s) 268, 395, 636
BsrI ACTGG 1 cut(s) 846
BssECI CCNNGG 6 cut(s) 123, 196, 367, 373, 374, 873
BssMI GATC 4 cut(s) 115, 159, 180, 231
BssNI GRCGYC 1 cut(s) 88
Bst6I CTCTTC 6 cut(s) 9, 57, 584, 626, 681, 815
BstACI GRCGYC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 31
BstBAI YACGTR 1 cut(s) 769
BstDEI CTNAG 3 cut(s) 315, 446, 833
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 3 cut(s) 526, 600, 787
BstFNI CGCG 3 cut(s) 114, 216, 369
BstH2I RGCGCY 1 cut(s) 904
BstHHI GCGC 2 cut(s) 25, 903
BstKTI GATC 4 cut(s) 118, 162, 183, 234
BstMBI GATC 4 cut(s) 115, 159, 180, 231
BstMCI CGRYCG 2 cut(s) 166, 367
BstMWI GCNNNNNNNGC 8 cut(s) 31, 82, 308, 369, 442, 451, 623, 772
BstSCI CCNGG 3 cut(s) 373, 374, 409
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 3 cut(s) 114, 216, 369
BstV1I GCAGC 4 cut(s) 393, 640, 762, 816
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 2 cut(s) 163, 364
Bsu15I ATCGAT 1 cut(s) 40
BsuRI GGCC 5 cut(s) 128, 165, 291, 366, 372
BsuTUI ATCGAT 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 526, 600, 787
BtsIMutI CAGTG 1 cut(s) 853
CciNI GCGGCCGC 1 cut(s) 364
CfoI GCGC 2 cut(s) 25, 903
Cfr13I GGNCC 4 cut(s) 210, 256, 290, 371
Cfr42I CCGCGG 1 cut(s) 370
Cfr9I CCCGGG 1 cut(s) 374
ClaI ATCGAT 1 cut(s) 40
CseI GACGC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 385, 841
CviAII CATG 1 cut(s) 151
CviQI GTAC 2 cut(s) 385, 841
DdeI CTNAG 3 cut(s) 315, 446, 833
DpnI GATC 4 cut(s) 117, 161, 182, 233
DpnII GATC 4 cut(s) 115, 159, 180, 231
EaeI YGGCCR 2 cut(s) 163, 364
EagI CGGCCG 2 cut(s) 163, 364
Eam1104I CTCTTC 6 cut(s) 9, 57, 584, 626, 681, 815
EarI CTCTTC 6 cut(s) 9, 57, 584, 626, 681, 815
EciI GGCGGA 4 cut(s) 286, 566, 584, 596
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 2 cut(s) 163, 364
Eco147I AGGCCT 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 2 cut(s) 210, 256
Eco47III AGCGCT 1 cut(s) 902
Eco52I CGGCCG 2 cut(s) 163, 364
Eco53kI GAGCTC 1 cut(s) 283
Eco72I CACGTG 1 cut(s) 769
Eco88I CYCGRG 2 cut(s) 197, 374
EcoICRI GAGCTC 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 285
FaeI CATG 1 cut(s) 154
FaiI YATR 8 cut(s) 94, 152, 468, 473, 475, 501, 507, 887
FalI AAGNNNNNCTT 2 cut(s) 873, 905
FaqI GGGAC 1 cut(s) 524
FatI CATG 1 cut(s) 150
FauI CCCGC 2 cut(s) 221, 629
FokI GGATG 3 cut(s) 533, 607, 794
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 2 cut(s) 492, 904
GlaI GCGC 2 cut(s) 24, 902
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 2 cut(s) 115, 830
HaeII RGCGCY 1 cut(s) 904
HaeIII GGCC 5 cut(s) 128, 165, 291, 366, 372
HapII CCGG 6 cut(s) 166, 208, 375, 411, 440, 779
HgaI GACGC 1 cut(s) 96
HhaI GCGC 2 cut(s) 25, 903
Hin1I GRCGYC 1 cut(s) 88
Hin1II CATG 1 cut(s) 154
Hin6I GCGC 2 cut(s) 23, 901
HinP1I GCGC 2 cut(s) 23, 901
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 2 cut(s) 42, 414
HpaII CCGG 6 cut(s) 166, 208, 375, 411, 440, 779
HphI GGTGA 2 cut(s) 640, 673
Hpy166II GTNNAC 2 cut(s) 766, 843
Hpy188I TCNGA 2 cut(s) 106, 287
Hpy188III TCNNGA 5 cut(s) 132, 184, 248, 430, 440
Hpy8I GTNNAC 2 cut(s) 766, 843
Hpy99I CGWCG 3 cut(s) 90, 230, 335
HpyAV CCTTC 2 cut(s) 247, 658
HpyCH4IV ACGT 1 cut(s) 768
HpyCH4V TGCA 2 cut(s) 656, 766
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 82, 308, 369, 442, 451, 623, 772
HpyF3I CTNAG 3 cut(s) 315, 446, 833
HpySE526I ACGT 1 cut(s) 768
Hsp92I GRCGYC 1 cut(s) 88
Hsp92II CATG 1 cut(s) 154
HspAI GCGC 2 cut(s) 23, 901
Kpn2I TCCGGA 1 cut(s) 439
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 4 cut(s) 115, 159, 180, 231
LguI GCTCTTC 1 cut(s) 626
LmnI GCTCC 8 cut(s) 202, 265, 280, 392, 421, 442, 826, 865
Lsp1109I GCAGC 4 cut(s) 393, 640, 762, 816
LweI GCATC 2 cut(s) 21, 478
MaeI CTAG 2 cut(s) 492, 904
MaeII ACGT 1 cut(s) 768
MaeIII GTNAC 1 cut(s) 8
MalI GATC 4 cut(s) 117, 161, 182, 233
MbiI CCGCTC 3 cut(s) 268, 395, 636
MboI GATC 4 cut(s) 115, 159, 180, 231
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 3 cut(s) 207, 285, 768
MluCI AATT 4 cut(s) 243, 295, 725, 854
MlyI GAGTC 1 cut(s) 423
MroI TCCGGA 1 cut(s) 439
MseI TTAA 2 cut(s) 891, 897
MspA1I CMGCKG 3 cut(s) 363, 369, 775
MspI CCGG 6 cut(s) 166, 208, 375, 411, 440, 779
MspR9I CCNGG 3 cut(s) 375, 376, 411
MvnI CGCG 3 cut(s) 114, 216, 369
MwoI GCNNNNNNNGC 8 cut(s) 31, 82, 308, 369, 442, 451, 623, 772
NciI CCSGG 3 cut(s) 375, 376, 411
NdeII GATC 4 cut(s) 115, 159, 180, 231
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 2 cut(s) 212, 373
NmeAIII GCCGAG 2 cut(s) 148, 202
NotI GCGGCCGC 1 cut(s) 364
PceI AGGCCT 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 626
PfeI GAWTC 1 cut(s) 42
PleI GAGTC 1 cut(s) 422
PmaCI CACGTG 1 cut(s) 769
PmlI CACGTG 1 cut(s) 769
PpsI GAGTC 1 cut(s) 422
Ppu21I YACGTR 1 cut(s) 769
Psp124BI GAGCTC 1 cut(s) 285
PspCI CACGTG 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 212, 373
PspPI GGNCC 4 cut(s) 210, 256, 290, 371
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 2 cut(s) 386, 842
RsaNI GTAC 2 cut(s) 385, 841
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 626
SaqAI TTAA 2 cut(s) 891, 897
Sau3AI GATC 4 cut(s) 115, 159, 180, 231
Sau96I GGNCC 4 cut(s) 210, 256, 290, 371
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 3 cut(s) 375, 376, 411
SduI GDGCHC 3 cut(s) 207, 285, 768
SfaNI GCATC 2 cut(s) 21, 478
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SinI GGWCC 2 cut(s) 210, 256
SmaI CCCGGG 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 4 cut(s) 243, 295, 725, 854
SseBI AGGCCT 1 cut(s) 128
SspI AATATT 1 cut(s) 340
SspMI CTAG 2 cut(s) 492, 904
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 1 cut(s) 128
StyD4I CCNGG 3 cut(s) 373, 374, 409
TaiI ACGT 1 cut(s) 771
TaqI TCGA 6 cut(s) 29, 40, 247, 330, 345, 429
TaqII GACCGA 2 cut(s) 338, 887
TasI AATT 4 cut(s) 243, 295, 725, 854
TatI WGTACW 1 cut(s) 840
TauI GCSGC 8 cut(s) 114, 142, 271, 366, 369, 372, 398, 775
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 2 cut(s) 891, 897
Tru9I TTAA 2 cut(s) 891, 897
TscAI CASTG 1 cut(s) 853
TseI GCWGC 4 cut(s) 406, 653, 775, 829
TspDTI ATGAA 2 cut(s) 34, 867
TspMI CCCGGG 1 cut(s) 374
TspRI CASTG 1 cut(s) 853
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 210, 256
XmaI CCCGGG 1 cut(s) 374
XspI CTAG 2 cut(s) 492, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.