RchiOBHm_Chr4g0411521

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006322
Physical Location & Seq
Reverse (-)
35343127 .. 35346858
3732 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38232

Sequence Viewer

Length: 909 bp
ATGGCTCGTAATGAAGAGAAAGCGCAGTCCATGCTGAATCGCTACATCACCCTCAAAAACGAAGAGAAGAAGAAGCCCCGAGAGCGCCGCCCTTTCCTGGCCTCCGAATGCCGCGACCTCGCCGAGGCCTCCAAGTGGCGCCAGCAGATCATGGGAGAGATCGGCCGCAAAGTCGCCGAGATCCAAAACGAAGGCCTCGGCGAGCACCGCCTCCGCGACCTCAACGACGAGATCAACAAGCTCATCCGCGAGAAGACTCACTGGGAGCGCCGCATCGTCGAGCTCGGCGGCCCCAATTACGCCAAGCATGCTCCTAAGATGACCGATCTCGACGGCAACATCGTCGACGTTCCGAACCCGAGCGGCCGCGGCGCCGGTTACCGGTACTTTGGCGCGGCGAAGAAGCTGCCGGGTGTCAGGGAGCTTTTCGAGAAGCCGCCGGAGCTGAGGAAGCGGCGGACGAGGTATGACATTTACAAGAGGATTGATGCCAGCTATTATGGGTACAGGGACGACGAGGATGGGGTTTTGGAGAAGCTGGAGGTTGTGGCGGAGGAGAAGATGAGGGCGGAGGCGGTGGCGGAGTGGTGGAGGTTGGAGGAGATAAGGAGGGAGGCCAAGAGGGCCGTGAAGAGTGGTGAAGTGGTGACGGTGGCGAAGGCCAAGGAGATTCTGGTGGAGGAGGAGGAGGAGGTGGTGGAAGAAGAGAGGCAGAGAGAGAGGGAGTTGTTCAAAGAGAAGAGTGAAAAAGAGATGGAGTTTGTCGCTCATGTGCCATTGCCGGATGACAAGGAGATTGAGAAGATGGTGCTTGAGAAGAAGAAGAAGGACTTGCTGAGTAGGTATGTCAGTGAGGAATTGATGCAGGACCAGACTGAGGCTAAGCAGATGCTTAATATTCAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

302

Amino Acids

35.7

Weight (kDa)

6.55

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 1 - 281 4.1e-91 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 138, 371
AccBSI CCGCTC 1 cut(s) 363
AccI GTMKAC 1 cut(s) 345
AccII CGCG 5 cut(s) 114, 216, 249, 369, 395
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 163, 364
AcyI GRCGYC 2 cut(s) 139, 372
AfaI GTAC 2 cut(s) 386, 506
AfiI CCNNNNNNNGG 5 cut(s) 97, 124, 135, 381, 877
AgeI ACCGGT 1 cut(s) 381
AgsI TTSAA 1 cut(s) 733
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
AluI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
Alw21I GWGCWC 2 cut(s) 207, 285
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 2 cut(s) 78, 358
AoxI GGCC 9 cut(s) 99, 126, 163, 193, 289, 364, 615, 624, 660
ApeKI GCWGC 1 cut(s) 406
AsiGI ACCGGT 1 cut(s) 381
AspLEI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
AspS9I GGNCC 3 cut(s) 290, 624, 868
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 40, 650, 658
AvaI CYCGRG 2 cut(s) 78, 358
AvaII GGWCC 1 cut(s) 868
BanI GGYRCC 2 cut(s) 138, 371
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 2 cut(s) 207, 285
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 393
BccI CCATC 3 cut(s) 515, 748, 799
BceAI ACGGC 2 cut(s) 349, 611
BcgI CGANNNNNNTGC 4 cut(s) 325, 359, 388, 422
BciT130I CCWGG 1 cut(s) 98
BcnI CCSGG 1 cut(s) 411
BfoI RGCGCY 4 cut(s) 88, 142, 271, 375
BglI GCCNNNNNGGC 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 882
Bme1390I CCNGG 2 cut(s) 98, 411
Bme18I GGWCC 1 cut(s) 868
BmeT110I CYCGRG 2 cut(s) 78, 358
BmgT120I GGNCC 3 cut(s) 290, 624, 868
BmiI GGNNCC 3 cut(s) 140, 292, 373
BmrFI CCNGG 2 cut(s) 98, 411
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 4 cut(s) 282, 478, 852, 879
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 446
Bpu1102I GCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 1 cut(s) 411
BsaHI GRCGYC 2 cut(s) 139, 372
BsaJI CCNNGG 4 cut(s) 123, 196, 367, 663
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 124, 135, 381, 877
Bse118I RCCGGY 2 cut(s) 374, 381
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 776
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 4 cut(s) 123, 196, 367, 663
BseGI GGATG 3 cut(s) 243, 526, 790
BseLI CCNNNNNNNGG 5 cut(s) 97, 124, 135, 381, 877
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 3 cut(s) 437, 827, 867
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 6 cut(s) 569, 614, 695, 698, 701, 704
BseX3I CGGCCG 2 cut(s) 163, 364
BseXI GCAGC 1 cut(s) 393
Bsh1236I CGCG 5 cut(s) 114, 216, 249, 369, 395
Bsh1285I CGRYCG 2 cut(s) 166, 367
BshFI GGCC 9 cut(s) 101, 128, 165, 195, 291, 366, 617, 626, 662
BshNI GGYRCC 2 cut(s) 138, 371
BshTI ACCGGT 1 cut(s) 381
BsiEI CGRYCG 2 cut(s) 166, 367
BsiHKAI GWGCWC 2 cut(s) 207, 285
BsiHKCI CYCGRG 2 cut(s) 78, 358
BsiSI CCGG 5 cut(s) 375, 382, 410, 440, 782
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 5 cut(s) 97, 124, 135, 381, 877
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 9 cut(s) 101, 128, 165, 195, 291, 366, 617, 626, 662
BsoBI CYCGRG 2 cut(s) 78, 358
Bsp1286I GDGCHC 2 cut(s) 207, 285
Bsp143I GATC 5 cut(s) 147, 159, 180, 231, 325
Bsp1720I GCTNAGC 1 cut(s) 882
BspANI GGCC 9 cut(s) 101, 128, 165, 195, 291, 366, 617, 626, 662
BspCNI CTCAG 3 cut(s) 438, 828, 868
BspFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BspLI GGNNCC 3 cut(s) 140, 292, 373
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 2 cut(s) 138, 371
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 776
BsrFI RCCGGY 2 cut(s) 374, 381
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 2 cut(s) 374, 381
BssECI CCNNGG 4 cut(s) 123, 196, 367, 663
BssMI GATC 5 cut(s) 147, 159, 180, 231, 325
BssNI GRCGYC 2 cut(s) 139, 372
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 5 cut(s) 9, 57, 626, 699, 734
BstACI GRCGYC 2 cut(s) 139, 372
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 4 cut(s) 143, 203, 309, 493
BstDEI CTNAG 5 cut(s) 315, 446, 836, 876, 882
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 243, 526, 790
BstFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstH2I RGCGCY 4 cut(s) 88, 142, 271, 375
BstHHI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
BstKTI GATC 5 cut(s) 150, 162, 183, 234, 328
BstMBI GATC 5 cut(s) 147, 159, 180, 231, 325
BstMCI CGRYCG 2 cut(s) 166, 367
BstMWI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 311
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 96, 409
BstUI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 2 cut(s) 163, 364
BsuRI GGCC 9 cut(s) 101, 128, 165, 195, 291, 366, 617, 626, 662
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 243, 526, 790
BtsIMutI CAGTG 2 cut(s) 259, 856
Cac8I GCNNGC 4 cut(s) 143, 203, 309, 493
CciNI GCGGCCGC 1 cut(s) 364
CfoI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Cfr10I RCCGGY 2 cut(s) 374, 381
Cfr13I GGNCC 3 cut(s) 290, 624, 868
Cfr42I CCGCGG 1 cut(s) 370
Csp6I GTAC 2 cut(s) 385, 505
CspAI ACCGGT 1 cut(s) 381
CviAII CATG 4 cut(s) 31, 151, 308, 770
CviQI GTAC 2 cut(s) 385, 505
DdeI CTNAG 5 cut(s) 315, 446, 836, 876, 882
DinI GGCGCC 2 cut(s) 140, 373
DpnI GATC 5 cut(s) 149, 161, 182, 233, 327
DpnII GATC 5 cut(s) 147, 159, 180, 231, 325
EaeI YGGCCR 2 cut(s) 163, 364
EagI CGGCCG 2 cut(s) 163, 364
Eam1104I CTCTTC 5 cut(s) 9, 57, 626, 699, 734
EarI CTCTTC 5 cut(s) 9, 57, 626, 699, 734
EciI GGCGGA 4 cut(s) 472, 566, 584, 596
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 2 cut(s) 163, 364
Eco130I CCWWGG 1 cut(s) 663
Eco147I AGGCCT 2 cut(s) 128, 195
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 868
Eco52I CGGCCG 2 cut(s) 163, 364
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 2 cut(s) 78, 358
Eco91I GGTNACC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 2 cut(s) 140, 373
EheI GGCGCC 2 cut(s) 140, 373
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 4 cut(s) 34, 154, 311, 773
FaiI YATR 7 cut(s) 32, 152, 309, 468, 501, 771, 846
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 1 cut(s) 524
FatI CATG 4 cut(s) 30, 150, 307, 769
FblI GTMKAC 1 cut(s) 345
FokI GGATG 3 cut(s) 230, 533, 797
FriOI GRGCYC 1 cut(s) 285
GlaI GCGC 6 cut(s) 24, 86, 140, 269, 373, 394
GsuI CTGGAG 1 cut(s) 560
HaeII RGCGCY 4 cut(s) 88, 142, 271, 375
HaeIII GGCC 9 cut(s) 101, 128, 165, 195, 291, 366, 617, 626, 662
HapII CCGG 5 cut(s) 375, 382, 410, 440, 782
HhaI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Hin1I GRCGYC 2 cut(s) 139, 372
Hin1II CATG 4 cut(s) 34, 154, 311, 773
Hin6I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HinP1I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 3 cut(s) 37, 256, 670
HpaII CCGG 5 cut(s) 375, 382, 410, 440, 782
HphI GGTGA 3 cut(s) 40, 650, 658
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 106, 354
Hpy188III TCNNGA 2 cut(s) 329, 430
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 6 cut(s) 230, 281, 335, 347, 350, 518
HpyAV CCTTC 3 cut(s) 185, 652, 820
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 1 cut(s) 865
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
HpyF3I CTNAG 5 cut(s) 315, 446, 836, 876, 882
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 2 cut(s) 139, 372
Hsp92II CATG 4 cut(s) 34, 154, 311, 773
HspAI GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
KasI GGCGCC 2 cut(s) 138, 371
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 5 cut(s) 147, 159, 180, 231, 325
LmnI GCTCC 4 cut(s) 265, 316, 421, 442
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 4 cut(s) 282, 478, 852, 879
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 377, 646
MalI GATC 5 cut(s) 149, 161, 182, 233, 327
MbiI CCGCTC 1 cut(s) 363
MboI GATC 5 cut(s) 147, 159, 180, 231, 325
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 207, 285
MluCI AATT 2 cut(s) 295, 857
Mly113I GGCGCC 2 cut(s) 139, 372
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 576
MseI TTAA 1 cut(s) 894
MspA1I CMGCKG 2 cut(s) 369, 904
MspI CCGG 5 cut(s) 375, 382, 410, 440, 782
MspR9I CCNGG 2 cut(s) 98, 411
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 5 cut(s) 114, 216, 249, 369, 395
MwoI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
NarI GGCGCC 2 cut(s) 139, 372
NciI CCSGG 1 cut(s) 411
NdeII GATC 5 cut(s) 147, 159, 180, 231, 325
NlaIII CATG 4 cut(s) 34, 154, 311, 773
NlaIV GGNNCC 3 cut(s) 140, 292, 373
NmeAIII GCCGAG 4 cut(s) 148, 177, 202, 264
NmuCI GTSAC 1 cut(s) 646
NotI GCGGCCGC 1 cut(s) 364
NspI RCATGY 1 cut(s) 311
PaeI GCATGC 1 cut(s) 311
PceI AGGCCT 2 cut(s) 128, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 37, 670
PinAI ACCGGT 1 cut(s) 381
PleI GAGTC 1 cut(s) 250
PluTI GGCGCC 2 cut(s) 142, 375
PpsI GAGTC 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 96
PspEI GGTNACC 1 cut(s) 377
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 140, 292, 373
PspPI GGNCC 3 cut(s) 290, 624, 868
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 2 cut(s) 386, 506
RsaNI GTAC 2 cut(s) 385, 505
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 1 cut(s) 370
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 894
Sau3AI GATC 5 cut(s) 147, 159, 180, 231, 325
Sau96I GGNCC 3 cut(s) 290, 624, 868
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 98, 411
SduI GDGCHC 2 cut(s) 207, 285
SfaNI GCATC 4 cut(s) 282, 478, 852, 879
SfiI GGCCNNNNNGGCC 1 cut(s) 623
SfoI GGCGCC 2 cut(s) 140, 373
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SgrDI CGTCGACG 1 cut(s) 344
SinI GGWCC 1 cut(s) 868
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
SphI GCATGC 1 cut(s) 311
Sse9I AATT 2 cut(s) 295, 857
SseBI AGGCCT 2 cut(s) 128, 195
SspDI GGCGCC 2 cut(s) 138, 371
SspI AATATT 1 cut(s) 898
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 2 cut(s) 128, 195
StyD4I CCNGG 2 cut(s) 96, 409
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 1 cut(s) 351
TaqI TCGA 4 cut(s) 279, 330, 345, 429
TaqII GACCGA 1 cut(s) 338
TasI AATT 2 cut(s) 295, 857
TfiI GAWTC 2 cut(s) 37, 670
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TscAI CASTG 2 cut(s) 266, 856
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 27
TspRI CASTG 2 cut(s) 266, 856
VpaK11BI GGWCC 1 cut(s) 868
XagI CCTNNNNNAGG 1 cut(s) 122
XceI RCATGY 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.