Rroxscaffold_3G00238560

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006322
Physical Location & Seq
Forward (+)
26761603 .. 26764213
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00238560.1

Sequence Viewer

Length: 912 bp
ATGGCTCGTAACGAAGAGAAAGCGCAGTCCATGCTGAATCGCTACATCACTCTCAAAAACGAAGAGAAGAAGAAGCCCCGAGAGCGCCGCCCTTTCCTCGCCTCCGAATGCCGCGACCTCGCCGAGGCCTCCAAGTGGCGCCAGCAGATCATGGGAGAGATCGGCCGCAAAGTCGCCGAGATCCAAAACGAAGGCCTCGGCGAGCACCGCCTCCGCGACCTCAACGACGACATCAACAAGCTCATCCGCGAGAAGACTCACTGGGAGCGCCGCATCGTCGAGCTCGGCGGCCCCAATTACGCCAAGCACGCTCCTAAGATGACCGATCTCGACGGCAACATCGTCGACGTCCCAAACCCTAGCGGCCGCGGCGCCGGTTACCGGTATTTTGGCGCGGCGAAGAAGCTGCCGGGAGTCAGGGAGCTTTTCGAGAAGCCGCCGGAGCTGAGGAAGCGGCGGACGAGGTATGACATTTACAAGAGGATTGATGCCAGCTACTATGGGTACAGGGACGACGAGGATGGGGTTTTGGAGAAGCTGGAGGTTGTGGCGGAGGAGAAGATGAGGGCGGAGGCGGTGGCGGAGTGGTGGAGGTTGGAGGAGATAAGGAGAGAGGCCAAGAGGGCGGTGAAGAGTGGCGAGGTGGTGACGGTGGCGAAGGTGAAGGAGATTCTGGTGGAGGAGGAGGAGGAGGAGGTGGTGGAAGAAGAGAGGCAGAGAGAGAGGGAGTTGTTGAAAGAGAAGAGTGAAAAAGAGATGGAGTTTGTGGCTCATGTGCCATTGCCGGATGACAAGGAGATTGAGAAGATGGTGCTTGAGAAGAAGAAGAAGGACTTGATGAGTAGGTATGTCAGTGAGGAATTGCTGCAGGACCAGAGTGAGGCTAAGCAGATGCTTAACATTAAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

303

Amino Acids

35.79

Weight (kDa)

6.55

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 1 - 282 1.7e-90 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 138, 371
AccI GTMKAC 1 cut(s) 345
AccII CGCG 5 cut(s) 114, 216, 249, 369, 395
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 2 cut(s) 163, 364
AcyI GRCGYC 3 cut(s) 139, 348, 372
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 4 cut(s) 124, 135, 381, 880
AgeI ACCGGT 1 cut(s) 381
AgsI TTSAA 1 cut(s) 736
AluBI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
AluI AGCT 7 cut(s) 241, 283, 406, 424, 445, 495, 538
Alw21I GWGCWC 2 cut(s) 207, 285
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 6 cut(s) 126, 163, 193, 289, 364, 615
ApeKI GCWGC 2 cut(s) 406, 865
AsiGI ACCGGT 1 cut(s) 381
AspLEI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
AspS9I GGNCC 2 cut(s) 290, 871
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 3 cut(s) 640, 658, 673
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 871
BanI GGYRCC 2 cut(s) 138, 371
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 2 cut(s) 207, 285
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 2 cut(s) 393, 852
BccI CCATC 3 cut(s) 515, 751, 802
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 4 cut(s) 325, 359, 388, 422
BcnI CCSGG 1 cut(s) 411
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 866
BfoI RGCGCY 4 cut(s) 88, 142, 271, 375
BglI GCCNNNNNGGC 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 885
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 1 cut(s) 871
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 290, 871
BmiI GGNNCC 3 cut(s) 140, 292, 373
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 3 cut(s) 282, 478, 882
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 446
Bpu1102I GCTNAGC 1 cut(s) 885
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 1 cut(s) 411
BsaHI GRCGYC 3 cut(s) 139, 348, 372
BsaJI CCNNGG 3 cut(s) 123, 196, 367
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 135, 381, 880
Bse118I RCCGGY 2 cut(s) 374, 381
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 779
BseDI CCNNGG 3 cut(s) 123, 196, 367
BseGI GGATG 3 cut(s) 243, 526, 793
BseLI CCNNNNNNNGG 4 cut(s) 124, 135, 381, 880
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 7 cut(s) 569, 614, 695, 698, 701, 704, 707
BseX3I CGGCCG 2 cut(s) 163, 364
BseXI GCAGC 2 cut(s) 393, 852
Bsh1236I CGCG 5 cut(s) 114, 216, 249, 369, 395
Bsh1285I CGRYCG 2 cut(s) 166, 367
BshFI GGCC 6 cut(s) 128, 165, 195, 291, 366, 617
BshNI GGYRCC 2 cut(s) 138, 371
BshTI ACCGGT 1 cut(s) 381
BsiEI CGRYCG 2 cut(s) 166, 367
BsiHKAI GWGCWC 2 cut(s) 207, 285
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 5 cut(s) 375, 382, 410, 440, 785
BslFI GGGAC 2 cut(s) 335, 524
BslI CCNNNNNNNGG 4 cut(s) 124, 135, 381, 880
BsmFI GGGAC 2 cut(s) 335, 524
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 6 cut(s) 128, 165, 195, 291, 366, 617
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 207, 285
Bsp143I GATC 4 cut(s) 147, 159, 180, 325
Bsp1720I GCTNAGC 1 cut(s) 885
BspANI GGCC 6 cut(s) 128, 165, 195, 291, 366, 617
BspCNI CTCAG 1 cut(s) 438
BspFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BspLI GGNNCC 3 cut(s) 140, 292, 373
BspMAI CTGCAG 1 cut(s) 870
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 2 cut(s) 138, 371
BsrDI GCAATG 1 cut(s) 779
BsrFI RCCGGY 2 cut(s) 374, 381
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 2 cut(s) 374, 381
BssECI CCNNGG 3 cut(s) 123, 196, 367
BssMI GATC 4 cut(s) 147, 159, 180, 325
BssNI GRCGYC 3 cut(s) 139, 348, 372
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 5 cut(s) 9, 57, 626, 702, 737
BstACI GRCGYC 3 cut(s) 139, 348, 372
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 4 cut(s) 143, 203, 309, 493
BstDEI CTNAG 3 cut(s) 315, 446, 885
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 243, 526, 793
BstFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstH2I RGCGCY 4 cut(s) 88, 142, 271, 375
BstHHI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
BstKTI GATC 4 cut(s) 150, 162, 183, 328
BstMBI GATC 4 cut(s) 147, 159, 180, 325
BstMCI CGRYCG 2 cut(s) 166, 367
BstMWI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 866
BstUI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstV1I GCAGC 2 cut(s) 393, 852
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 2 cut(s) 163, 364
BsuRI GGCC 6 cut(s) 128, 165, 195, 291, 366, 617
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 243, 526, 793
BtsIMutI CAGTG 2 cut(s) 259, 859
Cac8I GCNNGC 4 cut(s) 143, 203, 309, 493
CciNI GCGGCCGC 1 cut(s) 364
CfoI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Cfr10I RCCGGY 2 cut(s) 374, 381
Cfr13I GGNCC 2 cut(s) 290, 871
Cfr42I CCGCGG 1 cut(s) 370
Csp6I GTAC 1 cut(s) 505
CspAI ACCGGT 1 cut(s) 381
CviAII CATG 3 cut(s) 31, 151, 773
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 3 cut(s) 315, 446, 885
DinI GGCGCC 2 cut(s) 140, 373
DpnI GATC 4 cut(s) 149, 161, 182, 327
DpnII GATC 4 cut(s) 147, 159, 180, 325
EaeI YGGCCR 2 cut(s) 163, 364
EagI CGGCCG 2 cut(s) 163, 364
Eam1104I CTCTTC 5 cut(s) 9, 57, 626, 702, 737
EarI CTCTTC 5 cut(s) 9, 57, 626, 702, 737
EciI GGCGGA 4 cut(s) 472, 566, 584, 596
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 2 cut(s) 163, 364
Eco147I AGGCCT 2 cut(s) 128, 195
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 871
Eco52I CGGCCG 2 cut(s) 163, 364
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 2 cut(s) 140, 373
EheI GGCGCC 2 cut(s) 140, 373
FaeI CATG 3 cut(s) 34, 154, 776
FaiI YATR 6 cut(s) 32, 152, 468, 501, 774, 849
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 2 cut(s) 335, 524
FatI CATG 3 cut(s) 30, 150, 772
FblI GTMKAC 1 cut(s) 345
FokI GGATG 3 cut(s) 230, 533, 800
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 1 cut(s) 360
GlaI GCGC 6 cut(s) 24, 86, 140, 269, 373, 394
GsuI CTGGAG 1 cut(s) 560
HaeII RGCGCY 4 cut(s) 88, 142, 271, 375
HaeIII GGCC 6 cut(s) 128, 165, 195, 291, 366, 617
HapII CCGG 5 cut(s) 375, 382, 410, 440, 785
HhaI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Hin1I GRCGYC 3 cut(s) 139, 348, 372
Hin1II CATG 3 cut(s) 34, 154, 776
Hin6I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HinP1I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 4 cut(s) 37, 256, 414, 670
HpaII CCGG 5 cut(s) 375, 382, 410, 440, 785
HphI GGTGA 3 cut(s) 640, 658, 673
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 2 cut(s) 329, 430
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 6 cut(s) 230, 281, 335, 347, 350, 518
HpyAV CCTTC 4 cut(s) 185, 652, 658, 823
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 1 cut(s) 868
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
HpyF3I CTNAG 3 cut(s) 315, 446, 885
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 3 cut(s) 139, 348, 372
Hsp92II CATG 3 cut(s) 34, 154, 776
HspAI GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
KasI GGCGCC 2 cut(s) 138, 371
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 4 cut(s) 147, 159, 180, 325
LmnI GCTCC 4 cut(s) 265, 316, 421, 442
Lsp1109I GCAGC 2 cut(s) 393, 852
LweI GCATC 3 cut(s) 282, 478, 882
MaeI CTAG 1 cut(s) 360
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 3 cut(s) 8, 377, 646
MalI GATC 4 cut(s) 149, 161, 182, 327
MboI GATC 4 cut(s) 147, 159, 180, 325
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 207, 285
MluCI AATT 2 cut(s) 295, 860
Mly113I GGCGCC 2 cut(s) 139, 372
MlyI GAGTC 2 cut(s) 250, 423
MmeI TCCRAC 1 cut(s) 576
MseI TTAA 2 cut(s) 897, 903
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 5 cut(s) 375, 382, 410, 440, 785
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 113
MvnI CGCG 5 cut(s) 114, 216, 249, 369, 395
MwoI GCNNNNNNNGC 8 cut(s) 31, 82, 207, 308, 369, 442, 451, 623
NarI GGCGCC 2 cut(s) 139, 372
NciI CCSGG 1 cut(s) 411
NdeII GATC 4 cut(s) 147, 159, 180, 325
NlaIII CATG 3 cut(s) 34, 154, 776
NlaIV GGNNCC 3 cut(s) 140, 292, 373
NmeAIII GCCGAG 4 cut(s) 148, 177, 202, 264
NmuCI GTSAC 1 cut(s) 646
NotI GCGGCCGC 1 cut(s) 364
PceI AGGCCT 2 cut(s) 128, 195
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 37, 670
PinAI ACCGGT 1 cut(s) 381
PleI GAGTC 2 cut(s) 250, 422
PluTI GGCGCC 2 cut(s) 142, 375
PpsI GAGTC 2 cut(s) 250, 422
Psp124BI GAGCTC 1 cut(s) 285
PspEI GGTNACC 1 cut(s) 377
PspN4I GGNNCC 3 cut(s) 140, 292, 373
PspPI GGNCC 2 cut(s) 290, 871
PstI CTGCAG 1 cut(s) 870
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 1 cut(s) 370
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 2 cut(s) 897, 903
Sau3AI GATC 4 cut(s) 147, 159, 180, 325
Sau96I GGNCC 2 cut(s) 290, 871
SchI GAGTC 2 cut(s) 250, 423
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 2 cut(s) 207, 285
SfaNI GCATC 3 cut(s) 282, 478, 882
SfcI CTRYAG 1 cut(s) 866
SfoI GGCGCC 2 cut(s) 140, 373
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SgrDI CGTCGACG 1 cut(s) 344
SinI GGWCC 1 cut(s) 871
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 2 cut(s) 295, 860
SseBI AGGCCT 2 cut(s) 128, 195
SspDI GGCGCC 2 cut(s) 138, 371
SspMI CTAG 1 cut(s) 360
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 2 cut(s) 128, 195
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 1 cut(s) 351
TaqI TCGA 4 cut(s) 279, 330, 345, 429
TaqII GACCGA 1 cut(s) 338
TasI AATT 2 cut(s) 295, 860
TfiI GAWTC 2 cut(s) 37, 670
Tru1I TTAA 2 cut(s) 897, 903
Tru9I TTAA 2 cut(s) 897, 903
TscAI CASTG 2 cut(s) 266, 859
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 2 cut(s) 406, 865
Tsp45I GTSAC 1 cut(s) 646
TspRI CASTG 2 cut(s) 266, 859
VpaK11BI GGWCC 1 cut(s) 871
XagI CCTNNNNNAGG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 1 cut(s) 360
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.