Rorug04G0108400

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006322
Physical Location & Seq
Forward (+)
16888361 .. 16888792
432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0108400.1

Sequence Viewer

Length: 432 bp
ATGTGGACTGAGTGTTGTGTTCAGGGACCATCAAGGAGTGTTAAAAGGAGCCATGGCAGTCCCCCAGGTCGGGGGATAAGTGGATTACAACCAAGTTCAGTTGAAGCTCTTGCTTTGCTTCATGGCCTTCGGTTCGCTGGTCATGTGGGGTTTAAAAGATTGGAAGTAGAAGGTGATGCTCTGTCCATCATTAATTCTTTACAAGTTGATTGTGATGATTTAAGTCTTGAAGGACATCTCCTAGATGAAGTTAAATTTTTAATTAATTCTTTTGAGTTTTGTAGTATGCACTTTGTGAAGCGGGAAGGCAACAATGTTACTCACCGTCTTGCAAAAGAGGCATTGAAAGTCAGTCAACCTTTCCTTTGTTTAGAGTCAGGACCTGCTTGGCTCCTTCAGTGCGTCAGTATAGACTTTCAGTATGAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

143

Amino Acids

15.81

Weight (kDa)

5.83

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 29 - 114 7.5e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 391
AciI CCGC 1 cut(s) 301
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 380
AdeI CACNNNGTG 1 cut(s) 295
AfiI CCNNNNNNNGG 3 cut(s) 69, 70, 71
AgsI TTSAA 3 cut(s) 104, 230, 346
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 1 cut(s) 107
AluI AGCT 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 383
AoxI GGCC 1 cut(s) 124
ApoI RAATTY 1 cut(s) 254
AseI ATTAAT 2 cut(s) 192, 264
AspS9I GGNCC 2 cut(s) 26, 380
AsuHPI GGTGA 2 cut(s) 185, 314
AvaII GGWCC 2 cut(s) 26, 380
BccI CCATC 2 cut(s) 37, 194
BciT130I CCWGG 1 cut(s) 66
BfaI CTAG 1 cut(s) 242
BfuAI ACCTGC 1 cut(s) 391
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 2 cut(s) 26, 380
BmgT120I GGNCC 2 cut(s) 26, 380
BmiI GGNNCC 3 cut(s) 27, 50, 392
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 166
BsaJI CCNNGG 2 cut(s) 52, 64
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 70, 71
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 52, 64
BseLI CCNNNNNNNGG 3 cut(s) 69, 70, 71
BshFI GGCC 1 cut(s) 126
BslFI GGGAC 2 cut(s) 39, 45
BslI CCNNNNNNNGG 3 cut(s) 69, 70, 71
BsmFI GGGAC 2 cut(s) 39, 45
BsnI GGCC 1 cut(s) 126
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 1 cut(s) 301
BspANI GGCC 1 cut(s) 126
BspLI GGNNCC 3 cut(s) 27, 50, 392
BspMI ACCTGC 1 cut(s) 391
BssECI CCNNGG 2 cut(s) 52, 64
BssT1I CCWWGG 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 326
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BsuRI GGCC 1 cut(s) 126
BtgI CCRYGG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 404
BveI ACCTGC 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 26, 380
CseI GACGC 1 cut(s) 391
CviAII CATG 3 cut(s) 53, 122, 143
CviJI RGCY 4 cut(s) 51, 107, 126, 391
CviKI_1 RGCY 4 cut(s) 51, 107, 126, 391
DdeI CTNAG 1 cut(s) 9
DraI TTTAAA 1 cut(s) 154
DraIII CACNNNGTG 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 52
Eco47I GGWCC 2 cut(s) 26, 380
Eco57I CTGAAG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 380
EcoRII CCWGG 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 52
FaeI CATG 3 cut(s) 56, 125, 146
FaiI YATR 6 cut(s) 54, 123, 144, 287, 410, 423
FaqI GGGAC 2 cut(s) 39, 45
FatI CATG 3 cut(s) 52, 121, 142
FauI CCCGC 1 cut(s) 294
FspBI CTAG 1 cut(s) 242
HaeIII GGCC 1 cut(s) 126
HgaI GACGC 1 cut(s) 391
Hin1II CATG 3 cut(s) 56, 125, 146
HincII GTYRAC 1 cut(s) 356
HindII GTYRAC 1 cut(s) 356
HinfI GANTC 1 cut(s) 374
HphI GGTGA 2 cut(s) 185, 314
Hpy166II GTNNAC 2 cut(s) 6, 356
Hpy188III TCNNGA 2 cut(s) 227, 378
Hpy8I GTNNAC 2 cut(s) 6, 356
HpyAV CCTTC 5 cut(s) 137, 164, 224, 299, 404
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4V TGCA 2 cut(s) 289, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 1 cut(s) 9
Hsp92II CATG 3 cut(s) 56, 125, 146
LmnI GCTCC 2 cut(s) 48, 396
LpnPI CCDG 6 cut(s) 8, 51, 78, 123, 363, 396
LweI GCATC 1 cut(s) 166
MaeI CTAG 1 cut(s) 242
MaeIII GTNAC 1 cut(s) 316
MluCI AATT 4 cut(s) 193, 254, 261, 265
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 1 cut(s) 331
MseI TTAA 7 cut(s) 42, 153, 192, 221, 252, 260, 264
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 1 cut(s) 338
NcoI CCATGG 1 cut(s) 52
NlaIII CATG 3 cut(s) 56, 125, 146
NlaIV GGNNCC 3 cut(s) 27, 50, 392
PacI TTAATTAA 1 cut(s) 264
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 380
PshBI ATTAAT 2 cut(s) 192, 264
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 3 cut(s) 27, 50, 392
PspPI GGNCC 2 cut(s) 26, 380
PspPPI RGGWCCY 1 cut(s) 380
PstNI CAGNNNCTG 1 cut(s) 383
SaqAI TTAA 7 cut(s) 42, 153, 192, 221, 252, 260, 264
Sau96I GGNCC 2 cut(s) 26, 380
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 66
SetI ASST 5 cut(s) 70, 109, 175, 361, 385
SfaNI GCATC 1 cut(s) 166
SinI GGWCC 2 cut(s) 26, 380
Sse9I AATT 4 cut(s) 193, 254, 261, 265
SsiI CCGC 1 cut(s) 301
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 326
TasI AATT 4 cut(s) 193, 254, 261, 265
Tru1I TTAA 7 cut(s) 42, 153, 192, 221, 252, 260, 264
Tru9I TTAA 7 cut(s) 42, 153, 192, 221, 252, 260, 264
TscAI CASTG 1 cut(s) 404
TspDTI ATGAA 2 cut(s) 110, 261
TspRI CASTG 1 cut(s) 404
VpaK11BI GGWCC 2 cut(s) 26, 380
VspI ATTAAT 2 cut(s) 192, 264
XapI RAATTY 1 cut(s) 254
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.