Rh7CG360500

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006322
Physical Location & Seq
Forward (+)
44723185 .. 44728455
5271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG360500.1

Sequence Viewer

Length: 885 bp
ATGGCTCGTAACGAAGAGAAAGCGCAGTCCATGCTGAATCGCTACATCACTCTCAAAAACGAAGAGAAGAAGAAGCCCCGAGAGCGCCGCCCTTTCCTCGCCTCCGAATGCCGCGACCTCGCCGAGGCCTCCAAGTGGCGCCAGCAGATCATGGGAGAGATCGGCCGCAACGTCGCCGAGATCCAAAACGAAGGCCTCGGCGAGCACCGCCTCCGCGACCTCAACGACGAGATCAACAAGCTCATCCGCGAGAAGACTCACTGGGAGCGCCGCATCGTCGACCTCGGCGGCCCCAACTACGCCAAGCACGCTCCTAAGATGACCGATCTCGACGGCAACATCGTCGACGTTCCCAACCCTAGCGGCCGCGGCGCCGGTTACCGCTATTTTGGCGCGGCCAAGAAGCTGCCCGGTGTCAGGGAGCTTTTCGAGAAGCCGCCGGAGCTGAGGAAGCGGCGGACGAGGTATGACATTTACAAGAGGATTGATGCCAGCTATTATGGGTACAGGGACGACGAGGATGGGGTTTTGGAGAAGCTGGACGTTGTGGCGGAGTGGTGGAGGTTGGAGGAGATAAGGAGAGAGGCCAAGAGGGCGGTGAAGAGTGGCGAGGTGGTGACGGTGGCGAAGGCGAAGGAGATTCTGGTGGAGGAGGAGGAGGAGGTGGTGGAAGAAGAGAGGGAGTTGTTGAAAGAGAAGAGTGAAAAGGAGATGGAGTTTGTGGCTCATGTGCCATTGCCGGATGACAAGGAGATTGAGAAGATGGTGCTTGAGAAGAAGAAGAAGGACTTGCTCCGAAACATTGATAGTGGAATGTTTACCATAGACTGGCTCTTTGGGTCTGTTATATGTGTTGCGAGTTATAAAAACTTGTTAGCCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

294

Amino Acids

34.38

Weight (kDa)

6.53

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 1 - 266 1.7e-87 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AccB1I GGYRCC 2 cut(s) 138, 371
AccB7I CCANNNNNTGG 1 cut(s) 828
AccI GTMKAC 2 cut(s) 279, 345
AccII CGCG 5 cut(s) 114, 216, 249, 369, 395
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 3 cut(s) 163, 364, 396
AcyI GRCGYC 2 cut(s) 139, 372
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 4 cut(s) 124, 135, 417, 828
AgsI TTSAA 1 cut(s) 691
AluBI AGCT 6 cut(s) 241, 406, 424, 445, 495, 538
AluI AGCT 6 cut(s) 241, 406, 424, 445, 495, 538
Alw21I GWGCWC 1 cut(s) 207
AlwI GGATC 1 cut(s) 175
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 7 cut(s) 126, 163, 193, 289, 364, 396, 585
ApeKI GCWGC 1 cut(s) 406
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AspLEI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
AspS9I GGNCC 1 cut(s) 290
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 2 cut(s) 610, 628
AvaI CYCGRG 1 cut(s) 78
BanI GGYRCC 2 cut(s) 138, 371
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 207
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 393
BccI CCATC 3 cut(s) 515, 706, 757
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BcnI CCSGG 1 cut(s) 411
BfaI CTAG 1 cut(s) 360
BfoI RGCGCY 4 cut(s) 88, 142, 271, 375
BglI GCCNNNNNGGC 1 cut(s) 593
Bme1390I CCNGG 1 cut(s) 411
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 3 cut(s) 140, 292, 373
BmrFI CCNGG 1 cut(s) 411
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 2 cut(s) 282, 478
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 791
BpuMI CCSGG 1 cut(s) 411
BsaHI GRCGYC 2 cut(s) 139, 372
BsaJI CCNNGG 4 cut(s) 123, 196, 283, 367
BsaXI ACNNNNNCTCC 2 cut(s) 701, 731
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 135, 417, 828
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 2 cut(s) 266, 833
Bse3DI GCAATG 1 cut(s) 734
BseDI CCNNGG 4 cut(s) 123, 196, 283, 367
BseGI GGATG 3 cut(s) 243, 526, 748
BseLI CCNNNNNNNGG 4 cut(s) 124, 135, 417, 828
BseMI GCAATG 1 cut(s) 734
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 2 cut(s) 266, 833
BseRI GAGGAG 5 cut(s) 584, 665, 668, 671, 674
BseX3I CGGCCG 2 cut(s) 163, 364
BseXI GCAGC 1 cut(s) 393
Bsh1236I CGCG 5 cut(s) 114, 216, 249, 369, 395
Bsh1285I CGRYCG 2 cut(s) 166, 367
BshFI GGCC 7 cut(s) 128, 165, 195, 291, 366, 398, 587
BshNI GGYRCC 2 cut(s) 138, 371
BsiEI CGRYCG 2 cut(s) 166, 367
BsiHKAI GWGCWC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 4 cut(s) 375, 411, 440, 740
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 4 cut(s) 124, 135, 417, 828
BsmFI GGGAC 1 cut(s) 524
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 7 cut(s) 128, 165, 195, 291, 366, 398, 587
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 5 cut(s) 147, 159, 180, 231, 325
BspANI GGCC 7 cut(s) 128, 165, 195, 291, 366, 398, 587
BspCNI CTCAG 1 cut(s) 438
BspFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BspLI GGNNCC 3 cut(s) 140, 292, 373
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 2 cut(s) 138, 371
BsrDI GCAATG 1 cut(s) 734
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 2 cut(s) 266, 833
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 4 cut(s) 123, 196, 283, 367
BssMI GATC 5 cut(s) 147, 159, 180, 231, 325
BssNI GRCGYC 2 cut(s) 139, 372
Bst4CI ACNGT 1 cut(s) 622
Bst6I CTCTTC 5 cut(s) 9, 57, 596, 669, 692
BstACI GRCGYC 2 cut(s) 139, 372
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 4 cut(s) 143, 203, 309, 493
BstDEI CTNAG 2 cut(s) 315, 446
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 243, 526, 748
BstFNI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstH2I RGCGCY 4 cut(s) 88, 142, 271, 375
BstHHI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
BstKTI GATC 5 cut(s) 150, 162, 183, 234, 328
BstMBI GATC 5 cut(s) 147, 159, 180, 231, 325
BstMCI CGRYCG 2 cut(s) 166, 367
BstMWI GCNNNNNNNGC 9 cut(s) 31, 82, 207, 308, 369, 390, 442, 451, 593
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 409
BstUI CGCG 5 cut(s) 114, 216, 249, 369, 395
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 2 cut(s) 163, 364
BsuRI GGCC 7 cut(s) 128, 165, 195, 291, 366, 398, 587
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 3 cut(s) 243, 526, 748
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 143, 203, 309, 493
CciNI GCGGCCGC 1 cut(s) 364
CfoI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 290
Cfr42I CCGCGG 1 cut(s) 370
Csp6I GTAC 1 cut(s) 505
CviAII CATG 3 cut(s) 31, 151, 728
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 2 cut(s) 315, 446
DinI GGCGCC 2 cut(s) 140, 373
DpnI GATC 5 cut(s) 149, 161, 182, 233, 327
DpnII GATC 5 cut(s) 147, 159, 180, 231, 325
EaeI YGGCCR 3 cut(s) 163, 364, 396
EagI CGGCCG 2 cut(s) 163, 364
Eam1104I CTCTTC 5 cut(s) 9, 57, 596, 669, 692
EarI CTCTTC 5 cut(s) 9, 57, 596, 669, 692
EciI GGCGGA 2 cut(s) 472, 566
EclXI CGGCCG 2 cut(s) 163, 364
Eco147I AGGCCT 2 cut(s) 128, 195
Eco52I CGGCCG 2 cut(s) 163, 364
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 377
EgeI GGCGCC 2 cut(s) 140, 373
EheI GGCGCC 2 cut(s) 140, 373
FaeI CATG 3 cut(s) 34, 154, 731
FaiI YATR 9 cut(s) 32, 152, 468, 501, 729, 824, 848, 850, 864
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FaqI GGGAC 1 cut(s) 524
FatI CATG 3 cut(s) 30, 150, 727
FblI GTMKAC 2 cut(s) 279, 345
FokI GGATG 3 cut(s) 230, 533, 755
FspBI CTAG 1 cut(s) 360
GlaI GCGC 6 cut(s) 24, 86, 140, 269, 373, 394
HaeII RGCGCY 4 cut(s) 88, 142, 271, 375
HaeIII GGCC 7 cut(s) 128, 165, 195, 291, 366, 398, 587
HapII CCGG 4 cut(s) 375, 411, 440, 740
HhaI GCGC 6 cut(s) 25, 87, 141, 270, 374, 395
Hin1I GRCGYC 2 cut(s) 139, 372
Hin1II CATG 3 cut(s) 34, 154, 731
Hin6I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HinP1I GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
HincII GTYRAC 2 cut(s) 280, 346
HindII GTYRAC 2 cut(s) 280, 346
HinfI GANTC 3 cut(s) 37, 256, 640
HpaII CCGG 4 cut(s) 375, 411, 440, 740
HphI GGTGA 2 cut(s) 610, 628
Hpy166II GTNNAC 3 cut(s) 280, 346, 819
Hpy188I TCNGA 2 cut(s) 106, 797
Hpy188III TCNNGA 2 cut(s) 329, 430
Hpy8I GTNNAC 3 cut(s) 280, 346, 819
Hpy99I CGWCG 7 cut(s) 176, 230, 281, 335, 347, 350, 518
HpyAV CCTTC 4 cut(s) 185, 622, 628, 778
HpyCH4III ACNGT 1 cut(s) 622
HpyCH4IV ACGT 3 cut(s) 171, 348, 543
HpyF10VI GCNNNNNNNGC 9 cut(s) 31, 82, 207, 308, 369, 390, 442, 451, 593
HpyF3I CTNAG 2 cut(s) 315, 446
HpySE526I ACGT 3 cut(s) 171, 348, 543
Hsp92I GRCGYC 2 cut(s) 139, 372
Hsp92II CATG 3 cut(s) 34, 154, 731
HspAI GCGC 6 cut(s) 23, 85, 139, 268, 372, 393
KasI GGCGCC 2 cut(s) 138, 371
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 5 cut(s) 147, 159, 180, 231, 325
LmnI GCTCC 5 cut(s) 265, 316, 421, 442, 798
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 2 cut(s) 282, 478
MaeI CTAG 1 cut(s) 360
MaeII ACGT 3 cut(s) 171, 348, 543
MaeIII GTNAC 3 cut(s) 8, 377, 616
MalI GATC 5 cut(s) 149, 161, 182, 233, 327
MboI GATC 5 cut(s) 147, 159, 180, 231, 325
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 207
Mly113I GGCGCC 2 cut(s) 139, 372
MlyI GAGTC 1 cut(s) 250
MmeI TCCRAC 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 4 cut(s) 375, 411, 440, 740
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 113
MvnI CGCG 5 cut(s) 114, 216, 249, 369, 395
MwoI GCNNNNNNNGC 9 cut(s) 31, 82, 207, 308, 369, 390, 442, 451, 593
NarI GGCGCC 2 cut(s) 139, 372
NciI CCSGG 1 cut(s) 411
NdeII GATC 5 cut(s) 147, 159, 180, 231, 325
NlaIII CATG 3 cut(s) 34, 154, 731
NlaIV GGNNCC 3 cut(s) 140, 292, 373
NmeAIII GCCGAG 4 cut(s) 148, 177, 202, 264
NmuCI GTSAC 1 cut(s) 616
NotI GCGGCCGC 1 cut(s) 364
PceI AGGCCT 2 cut(s) 128, 195
PcsI WCGNNNNNNNCGW 2 cut(s) 168, 339
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 37, 640
PflMI CCANNNNNTGG 1 cut(s) 828
PleI GAGTC 1 cut(s) 250
PluTI GGCGCC 2 cut(s) 142, 375
PpsI GAGTC 1 cut(s) 250
PsiI TTATAA 1 cut(s) 864
PspEI GGTNACC 1 cut(s) 377
PspN4I GGNNCC 3 cut(s) 140, 292, 373
PspPI GGNCC 1 cut(s) 290
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacII CCGCGG 1 cut(s) 370
SalI GTCGAC 2 cut(s) 278, 344
Sau3AI GATC 5 cut(s) 147, 159, 180, 231, 325
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 2 cut(s) 282, 478
SfoI GGCGCC 2 cut(s) 140, 373
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SgrDI CGTCGACG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SseBI AGGCCT 2 cut(s) 128, 195
SspDI GGCGCC 2 cut(s) 138, 371
SspMI CTAG 1 cut(s) 360
StuI AGGCCT 2 cut(s) 128, 195
StyD4I CCNGG 1 cut(s) 409
TaaI ACNGT 1 cut(s) 622
TaiI ACGT 3 cut(s) 174, 351, 546
TaqI TCGA 4 cut(s) 279, 330, 345, 429
TaqII GACCGA 1 cut(s) 338
TfiI GAWTC 2 cut(s) 37, 640
TscAI CASTG 1 cut(s) 266
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 616
TspRI CASTG 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 828
XagI CCTNNNNNAGG 1 cut(s) 122
XmiI GTMKAC 2 cut(s) 279, 345
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.