RLG00000002170

pre-mRNA-splicing factor ISY1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006322
Physical Location & Seq
Forward (+)
26692864 .. 26693425
562 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002170

Sequence Viewer

Length: 489 bp
ATGACCGATCTCGACGGCAACATTGTCGACGTTCCCAACCCTAGCGGCCGCGGCGCCGGTTACCGCTATTTTGGCGCGGCGAAGAAGCTGCCCGGTGTCAGGGAGCTTTTCGAGAAGCCGCCAGATCTGAGGAAGCGGGACGAGCTGGAGCTTGTGGCGGAGTGGTGGAGGTTGGAGGAGATAAGGAGGGAGGCCAAGAGGGCGGTGAAGAGTGGCCAAGTGGTGACGGTGGCCAAGGCGAAGGAGATTCTGGTGGAGGAGGAAGAGGAGGTGGTGGAAGAAGAGAGGCAGAGAGAGATGGAGTTATTGAAAGACAAGAGTGAAAAGGAGATGGAGTTTGTGGCTCATGTGCCATTGCCGGACGACAAGGAGATTGAGAAGATGGTGCTTGAGAAGACGAAGAAGAAGGACTTGATGAGTAGGTATGTCAGTGAGGAATTGCTGCAGGACCAGACTGAGGCTAAGCAGATGCTTAACATTAAGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
Isy1

Protein Analysis

163

Amino Acids

18.95

Weight (kDa)

5.18

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Isy1 PF06246 2 - 140 1.2e-13 Isy1-like splicing family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AccI GTMKAC 1 cut(s) 27
AccII CGCG 2 cut(s) 51, 77
AcoI YGGCCR 3 cut(s) 46, 214, 231
AcyI GRCGYC 1 cut(s) 54
AfiI CCNNNNNNNGG 2 cut(s) 99, 457
AgsI TTSAA 1 cut(s) 310
AluBI AGCT 4 cut(s) 88, 106, 145, 151
AluI AGCT 4 cut(s) 88, 106, 145, 151
AoxI GGCC 4 cut(s) 46, 192, 214, 231
ApeKI GCWGC 2 cut(s) 88, 442
AspLEI GCGC 2 cut(s) 56, 77
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 217, 235
AvaII GGWCC 1 cut(s) 448
BalI TGGCCA 2 cut(s) 216, 233
BanI GGYRCC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 401
BbvI GCAGC 2 cut(s) 75, 429
BccI CCATC 3 cut(s) 292, 325, 376
BceAI ACGGC 1 cut(s) 31
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 70, 104
BcnI CCSGG 1 cut(s) 93
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 200
BglII AGATCT 1 cut(s) 124
BisI GCNGC 7 cut(s) 46, 49, 52, 78, 89, 119, 443
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 7 cut(s) 47, 50, 53, 79, 90, 120, 444
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 1 cut(s) 448
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 167
Bpu1102I GCTNAGC 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 49, 234
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 457
Bse118I RCCGGY 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 49, 234
BseLI CCNNNNNNNGG 2 cut(s) 99, 457
BseMI GCAATG 1 cut(s) 353
BseMII CTCAG 2 cut(s) 119, 447
BseRI GAGGAG 3 cut(s) 191, 272, 281
BseX3I CGGCCG 1 cut(s) 46
BseXI GCAGC 2 cut(s) 75, 429
Bsh1236I CGCG 2 cut(s) 51, 77
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 4 cut(s) 48, 194, 216, 233
BshNI GGYRCC 1 cut(s) 53
BsiEI CGRYCG 1 cut(s) 49
BsiSI CCGG 3 cut(s) 57, 93, 359
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 99, 457
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 4 cut(s) 48, 194, 216, 233
Bsp143I GATC 2 cut(s) 7, 124
Bsp1720I GCTNAGC 1 cut(s) 462
BspANI GGCC 4 cut(s) 48, 194, 216, 233
BspCNI CTCAG 2 cut(s) 120, 448
BspFNI CGCG 2 cut(s) 51, 77
BspLI GGNNCC 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 53
BsrDI GCAATG 1 cut(s) 353
BsrFI RCCGGY 1 cut(s) 56
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 2 cut(s) 49, 234
BssMI GATC 2 cut(s) 7, 124
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 229
Bst6I CTCTTC 3 cut(s) 203, 258, 276
BstACI GRCGYC 1 cut(s) 54
BstDEI CTNAG 3 cut(s) 128, 456, 462
BstDSI CCRYGG 1 cut(s) 49
BstEII GGTNACC 1 cut(s) 59
BstFNI CGCG 2 cut(s) 51, 77
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 2 cut(s) 56, 77
BstKTI GATC 2 cut(s) 10, 127
BstMBI GATC 2 cut(s) 7, 124
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 4 cut(s) 51, 72, 142, 200
BstPI GGTNACC 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 443
BstUI CGCG 2 cut(s) 51, 77
BstV1I GCAGC 2 cut(s) 75, 429
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZI CGGCCG 1 cut(s) 46
BsuRI GGCC 4 cut(s) 48, 194, 216, 233
BtgI CCRYGG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 436
CciNI GCGGCCGC 1 cut(s) 46
CfoI GCGC 2 cut(s) 56, 77
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 448
Cfr42I CCGCGG 1 cut(s) 52
CviAII CATG 1 cut(s) 347
DdeI CTNAG 3 cut(s) 128, 456, 462
DinI GGCGCC 1 cut(s) 55
DpnI GATC 2 cut(s) 9, 126
DpnII GATC 2 cut(s) 7, 124
EaeI YGGCCR 3 cut(s) 46, 214, 231
EagI CGGCCG 1 cut(s) 46
Eam1104I CTCTTC 3 cut(s) 203, 258, 276
EarI CTCTTC 3 cut(s) 203, 258, 276
EciI GGCGGA 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 448
Eco52I CGGCCG 1 cut(s) 46
Eco91I GGTNACC 1 cut(s) 59
EcoO65I GGTNACC 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 234
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 1 cut(s) 350
FaiI YATR 2 cut(s) 348, 426
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 152
FatI CATG 1 cut(s) 346
FauI CCCGC 1 cut(s) 129
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 7 cut(s) 46, 49, 52, 78, 89, 119, 443
Fsp4HI GCNGC 7 cut(s) 46, 49, 52, 78, 89, 119, 443
FspBI CTAG 1 cut(s) 42
GlaI GCGC 2 cut(s) 55, 76
GluI GCNGC 7 cut(s) 46, 49, 52, 78, 89, 119, 443
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 4 cut(s) 48, 194, 216, 233
HapII CCGG 3 cut(s) 57, 93, 359
HhaI GCGC 2 cut(s) 56, 77
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 1 cut(s) 350
Hin6I GCGC 2 cut(s) 54, 75
HinP1I GCGC 2 cut(s) 54, 75
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 1 cut(s) 247
HpaII CCGG 3 cut(s) 57, 93, 359
HphI GGTGA 2 cut(s) 217, 235
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 2 cut(s) 11, 112
Hpy8I GTNNAC 1 cut(s) 28
Hpy99I CGWCG 2 cut(s) 17, 32
HpyAV CCTTC 2 cut(s) 235, 400
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 1 cut(s) 445
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 72, 142, 200
HpyF3I CTNAG 3 cut(s) 128, 456, 462
HpySE526I ACGT 1 cut(s) 30
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 1 cut(s) 350
HspAI GCGC 2 cut(s) 54, 75
KasI GGCGCC 1 cut(s) 53
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 2 cut(s) 7, 124
LmnI GCTCC 2 cut(s) 103, 148
LpnPI CCDG 9 cut(s) 70, 85, 106, 131, 135, 236, 372, 431, 464
Lsp1109I GCAGC 2 cut(s) 75, 429
LweI GCATC 1 cut(s) 459
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 2 cut(s) 59, 223
MalI GATC 2 cut(s) 9, 126
MboI GATC 2 cut(s) 7, 124
MboII GAAGA 9 cut(s) 94, 220, 275, 290, 293, 391, 406, 412, 415
MflI RGATCY 1 cut(s) 124
MlsI TGGCCA 2 cut(s) 216, 233
MluCI AATT 1 cut(s) 437
MluNI TGGCCA 2 cut(s) 216, 233
Mly113I GGCGCC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 153
Mox20I TGGCCA 2 cut(s) 216, 233
MscI TGGCCA 2 cut(s) 216, 233
MseI TTAA 2 cut(s) 474, 480
Msp20I TGGCCA 2 cut(s) 216, 233
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 3 cut(s) 57, 93, 359
MspR9I CCNGG 1 cut(s) 93
MvnI CGCG 2 cut(s) 51, 77
MwoI GCNNNNNNNGC 4 cut(s) 51, 72, 142, 200
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 1 cut(s) 93
NdeII GATC 2 cut(s) 7, 124
NlaIII CATG 1 cut(s) 350
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 223
NotI GCGGCCGC 1 cut(s) 46
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 7 cut(s) 47, 50, 53, 79, 90, 120, 444
PluTI GGCGCC 1 cut(s) 57
PspEI GGTNACC 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 448
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 124
SacII CCGCGG 1 cut(s) 52
SalI GTCGAC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 474, 480
SatI GCNGC 7 cut(s) 46, 49, 52, 78, 89, 119, 443
Sau3AI GATC 2 cut(s) 7, 124
Sau96I GGNCC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 93
SetI ASST 8 cut(s) 33, 90, 108, 147, 153, 173, 273, 425
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 443
SfoI GGCGCC 1 cut(s) 55
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 1 cut(s) 437
SspDI GGCGCC 1 cut(s) 53
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 91
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 33
TaqI TCGA 3 cut(s) 12, 27, 111
TaqII GACCGA 1 cut(s) 20
TasI AATT 1 cut(s) 437
TauI GCSGC 5 cut(s) 48, 51, 54, 80, 121
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 2 cut(s) 474, 480
Tru9I TTAA 2 cut(s) 474, 480
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 88, 442
Tsp45I GTSAC 1 cut(s) 223
TspRI CASTG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 27
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.