FvH4_4g12880

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007361
Physical Location & Seq
Forward (+)
16541818 .. 16547954
6137 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12880.t4

Sequence Viewer

Length: 942 bp
ATGAACTTGGGCAAACCCAATTCCCCAAATGCTCCCTCACCACCACAACTCAACCTCCACCAAGCCCTCAATTCCCTCCAATCCCATTGCTCCGGTCTCCTCCACAACCTCTCCTCCCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCACCTCCAGTCAGCTCTCTCCAACCTCCAATCCCAAGCTAAAACCGCATTCCACGCCGGCTTTTCGGGCTTTGATTCCTCCCATCAGAGCCCGGCTTGGGCTCGAATAGCTGTGAACAACAAAACCCAGCTGCCAAATGAGGGGCCCAGAGCTGCCATGTCGACGGAGGCCATCGAGGAGAGGCTGGCTGGCGTGCCGGTCTATGCTCTTAGCAATGCGGCTGAGGAGTTCGTGCTGGTTTCGGGTGCTTCGAGTGGGAAGAGTCTTGGGTTGTTTTGTTTTAAGAAGGAAGACGCTGAGTCACTTCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTGGTGGCTGTGGCCTTGAATAAGGTTTTCCAGCTCAAGGTTGATGGGGTGGCCTTTCGGTTGATTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAAGCTGGATCTTCTGAAGATGGCTTTTTTGGGGTGCCAGTTTTCCAGTCTAGGAGCTTGATTCTAAGAAGCCAAAGCAAGAGCTATCGTCCAGTATTTTTCAGGAAGGAGGATTTAGAAAACTCACTATCAAGAGCTTCTCGGGATCAGAAACAATTGAATCCTTTACTTAGACCAGGGGATATTCAGGTTGCTGTTCTTGAGGATGTAATAAAGGGGATGAAGGGTTCCACACCGGTCTGGGATGATGTTGTTTTCATCCCTCCTGGATTTGATATCTCTACTGATCCCACCAAGCAACAGGCAGATGCTGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

314

Amino Acids

33.91

Weight (kDa)

8.79

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 58 - 223 2.3e-54 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 655
AccI GTMKAC 1 cut(s) 320
AciI CCGC 3 cut(s) 204, 377, 504
AclWI GGATC 3 cut(s) 637, 774, 902
AcuI CTGAAG 1 cut(s) 657
AfiI CCNNNNNNNGG 3 cut(s) 256, 299, 550
AflII CTTAAG 1 cut(s) 608
AgeI ACCGGT 1 cut(s) 856
AgsI TTSAA 3 cut(s) 133, 532, 781
AhdI GACNNNNNGTC 1 cut(s) 457
AjnI CCWGG 2 cut(s) 796, 886
AloI GAACNNNNNNTCC 2 cut(s) 832, 864
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 3 cut(s) 637, 774, 902
AlwNI CAGNNNCTG 1 cut(s) 932
Ama87I CYCGRG 1 cut(s) 762
AoxI GGCC 4 cut(s) 302, 327, 525, 564
ApaI GGGCCC 1 cut(s) 306
ApeKI GCWGC 3 cut(s) 289, 311, 932
AsiGI ACCGGT 1 cut(s) 856
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 4 cut(s) 302, 303, 487, 507
AsuC2I CCSGG 2 cut(s) 251, 492
AsuHPI GGTGA 1 cut(s) 30
AvaI CYCGRG 1 cut(s) 762
AvaII GGWCC 2 cut(s) 487, 507
BaeGI GKGCMC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 655
BanII GRGCYC 3 cut(s) 251, 262, 306
BbsI GAAGAC 2 cut(s) 456, 579
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 276, 298, 919
BccI CCATC 5 cut(s) 126, 249, 338, 551, 635
BciT130I CCWGG 2 cut(s) 798, 888
BcnI CCSGG 2 cut(s) 251, 492
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 1 cut(s) 672
BfrI CTTAAG 1 cut(s) 608
BisI GCNGC 4 cut(s) 290, 312, 378, 933
BlsI GCNGC 4 cut(s) 291, 313, 379, 934
Bme1390I CCNGG 4 cut(s) 251, 492, 798, 888
Bme18I GGWCC 2 cut(s) 487, 507
BmeRI GACNNNNNGTC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 762
BmgT120I GGNCC 4 cut(s) 302, 303, 487, 507
BmiI GGNNCC 6 cut(s) 303, 304, 489, 508, 657, 850
BmrFI CCNGG 4 cut(s) 251, 492, 798, 888
BmsI GCATC 1 cut(s) 919
BpiI GAAGAC 2 cut(s) 456, 579
BpmI CTGGAG 1 cut(s) 149
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 4 cut(s) 488, 533, 576, 842
BpuMI CCSGG 2 cut(s) 251, 492
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 490, 797
BsaWI WCCGGW 2 cut(s) 92, 856
BsaXI ACNNNNNCTCC 6 cut(s) 39, 69, 95, 98, 125, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 299, 550
Bse118I RCCGGY 3 cut(s) 215, 355, 856
Bse1I ACTGG 4 cut(s) 166, 659, 667, 713
Bse3DI GCAATG 2 cut(s) 85, 379
BseBI CCWGG 2 cut(s) 798, 888
BseDI CCNNGG 2 cut(s) 490, 797
BseGI GGATG 4 cut(s) 832, 846, 871, 879
BseLI CCNNNNNNNGG 3 cut(s) 256, 299, 550
BseMI GCAATG 2 cut(s) 85, 379
BseMII CTCAG 3 cut(s) 372, 447, 573
BseNI ACTGG 4 cut(s) 166, 659, 667, 713
BseRI GAGGAG 4 cut(s) 89, 103, 350, 398
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 3 cut(s) 276, 298, 919
BseYI CCCAGC 1 cut(s) 285
BshFI GGCC 4 cut(s) 304, 329, 527, 566
BshNI GGYRCC 1 cut(s) 655
BshTI ACCGGT 1 cut(s) 856
BsiHKCI CYCGRG 1 cut(s) 762
BsiSI CCGG 6 cut(s) 93, 216, 251, 356, 492, 857
BslI CCNNNNNNNGG 3 cut(s) 256, 299, 550
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 3 cut(s) 206, 501, 607
BsnI GGCC 4 cut(s) 304, 329, 527, 566
Bso31I GGTCTC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 762
Bsp120I GGGCCC 1 cut(s) 302
Bsp1286I GDGCHC 3 cut(s) 251, 262, 306
Bsp143I GATC 3 cut(s) 629, 766, 907
BspACI CCGC 3 cut(s) 204, 377, 504
BspANI GGCC 4 cut(s) 304, 329, 527, 566
BspCNI CTCAG 3 cut(s) 373, 448, 574
BspLI GGNNCC 6 cut(s) 303, 304, 489, 508, 657, 850
BspPI GGATC 3 cut(s) 637, 774, 902
BspT107I GGYRCC 1 cut(s) 655
BspTI CTTAAG 1 cut(s) 608
BspTNI GGTCTC 1 cut(s) 101
BsrDI GCAATG 2 cut(s) 85, 379
BsrFI RCCGGY 3 cut(s) 215, 355, 856
BsrI ACTGG 4 cut(s) 166, 659, 667, 713
BssAI RCCGGY 3 cut(s) 215, 355, 856
BssECI CCNNGG 2 cut(s) 490, 797
BssMI GATC 3 cut(s) 629, 766, 907
Bst2UI CCWGG 2 cut(s) 798, 888
Bst6I CTCTTC 2 cut(s) 134, 413
BstAFI CTTAAG 1 cut(s) 608
BstAPI GCANNNNNTGC 1 cut(s) 932
BstC8I GCNNGC 4 cut(s) 217, 345, 349, 353
BstDEI CTNAG 6 cut(s) 368, 381, 456, 582, 686, 791
BstF5I GGATG 4 cut(s) 832, 846, 871, 879
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 3 cut(s) 632, 769, 910
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 3 cut(s) 629, 766, 907
BstMWI GCNNNNNNNGC 5 cut(s) 203, 212, 225, 266, 932
BstNI CCWGG 2 cut(s) 798, 888
BstSCI CCNGG 4 cut(s) 249, 490, 796, 886
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 276, 298, 919
BstV2I GAAGAC 2 cut(s) 456, 579
BstX2I RGATCY 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 517
BstYI RGATCY 1 cut(s) 629
BsuRI GGCC 4 cut(s) 304, 329, 527, 566
BtsCI GGATG 4 cut(s) 832, 846, 871, 879
Cac8I GCNNGC 4 cut(s) 217, 345, 349, 353
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 1 cut(s) 607
Cfr10I RCCGGY 3 cut(s) 215, 355, 856
Cfr13I GGNCC 4 cut(s) 302, 303, 487, 507
CseI GACGC 1 cut(s) 461
CspAI ACCGGT 1 cut(s) 856
CviAII CATG 1 cut(s) 316
DdeI CTNAG 6 cut(s) 368, 381, 456, 582, 686, 791
DpnI GATC 3 cut(s) 631, 768, 909
DpnII GATC 3 cut(s) 629, 766, 907
DriI GACNNNNNGTC 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 134, 413
Eam1105I GACNNNNNGTC 1 cut(s) 457
EarI CTCTTC 2 cut(s) 134, 413
Eco24I GRGCYC 3 cut(s) 251, 262, 306
Eco31I GGTCTC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 898
Eco47I GGWCC 2 cut(s) 487, 507
Eco57I CTGAAG 1 cut(s) 657
Eco88I CYCGRG 1 cut(s) 762
EcoO109I RGGNCCY 1 cut(s) 302
EcoRII CCWGG 2 cut(s) 796, 886
EcoRV GATATC 1 cut(s) 898
EcoT38I GRGCYC 3 cut(s) 251, 262, 306
FaeI CATG 1 cut(s) 319
FaiI YATR 4 cut(s) 317, 363, 474, 485
FatI CATG 1 cut(s) 315
FauI CCCGC 1 cut(s) 497
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 4 cut(s) 290, 312, 378, 933
FokI GGATG 4 cut(s) 839, 853, 866, 878
FriOI GRGCYC 3 cut(s) 251, 262, 306
Fsp4HI GCNGC 4 cut(s) 290, 312, 378, 933
FspBI CTAG 1 cut(s) 672
GlaI GCGC 1 cut(s) 606
GluI GCNGC 4 cut(s) 290, 312, 378, 933
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 4 cut(s) 304, 329, 527, 566
HapII CCGG 6 cut(s) 93, 216, 251, 356, 492, 857
HgaI GACGC 1 cut(s) 461
HhaI GCGC 1 cut(s) 607
Hin1II CATG 1 cut(s) 319
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HinfI GANTC 7 cut(s) 233, 421, 458, 577, 584, 682, 781
HpaII CCGG 6 cut(s) 93, 216, 251, 356, 492, 857
HphI GGTGA 1 cut(s) 30
Hpy166II GTNNAC 2 cut(s) 274, 321
Hpy188I TCNGA 3 cut(s) 246, 637, 771
Hpy188III TCNNGA 7 cut(s) 467, 581, 598, 724, 753, 764, 821
Hpy8I GTNNAC 2 cut(s) 274, 321
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 5 cut(s) 153, 439, 610, 721, 838
HpyCH4V TGCA 1 cut(s) 935
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 212, 225, 266, 932
HpyF3I CTNAG 6 cut(s) 368, 381, 456, 582, 686, 791
Hsp92II CATG 1 cut(s) 319
HspAI GCGC 1 cut(s) 605
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 3 cut(s) 629, 766, 907
LmnI GCTCC 3 cut(s) 37, 95, 675
Lsp1109I GCAGC 3 cut(s) 276, 298, 919
LweI GCATC 1 cut(s) 919
MaeI CTAG 1 cut(s) 672
MaeIII GTNAC 1 cut(s) 459
MalI GATC 3 cut(s) 631, 768, 909
MboI GATC 3 cut(s) 629, 766, 907
MboII GAAGA 7 cut(s) 121, 138, 430, 461, 579, 624, 650
MfeI CAATTG 1 cut(s) 776
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 3 cut(s) 251, 262, 306
MluCI AATT 3 cut(s) 19, 70, 776
MlyI GAGTC 3 cut(s) 430, 467, 593
MmeI TCCRAC 1 cut(s) 204
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 2 cut(s) 441, 609
MspA1I CMGCKG 1 cut(s) 289
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 6 cut(s) 93, 216, 251, 356, 492, 857
MspR9I CCNGG 4 cut(s) 251, 492, 798, 888
MunI CAATTG 1 cut(s) 776
Mva1269I GAATGC 3 cut(s) 206, 501, 607
MvaI CCWGG 2 cut(s) 798, 888
MwoI GCNNNNNNNGC 5 cut(s) 203, 212, 225, 266, 932
NaeI GCCGGC 1 cut(s) 217
NciI CCSGG 2 cut(s) 251, 492
NdeII GATC 3 cut(s) 629, 766, 907
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 1 cut(s) 319
NlaIV GGNNCC 6 cut(s) 303, 304, 489, 508, 657, 850
NmuCI GTSAC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 1 cut(s) 407
PctI GAATGC 3 cut(s) 206, 501, 607
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 4 cut(s) 233, 577, 682, 781
PfoI TCCNGGA 1 cut(s) 886
PinAI ACCGGT 1 cut(s) 856
PkrI GCNGC 4 cut(s) 291, 313, 379, 934
PleI GAGTC 3 cut(s) 429, 466, 592
PpsI GAGTC 3 cut(s) 429, 466, 592
Psp6I CCWGG 2 cut(s) 796, 886
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 2 cut(s) 796, 886
PspN4I GGNNCC 6 cut(s) 303, 304, 489, 508, 657, 850
PspOMI GGGCCC 1 cut(s) 302
PspPI GGNCC 4 cut(s) 302, 303, 487, 507
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 629
PvuII CAGCTG 1 cut(s) 289
SalI GTCGAC 1 cut(s) 319
SaqAI TTAA 2 cut(s) 441, 609
SatI GCNGC 4 cut(s) 290, 312, 378, 933
Sau3AI GATC 3 cut(s) 629, 766, 907
Sau96I GGNCC 4 cut(s) 302, 303, 487, 507
SchI GAGTC 3 cut(s) 430, 467, 593
ScrFI CCNGG 4 cut(s) 251, 492, 798, 888
SduI GDGCHC 3 cut(s) 251, 262, 306
SfaNI GCATC 1 cut(s) 919
SinI GGWCC 2 cut(s) 487, 507
SmlI CTYRAG 5 cut(s) 467, 548, 591, 608, 821
SmoI CTYRAG 5 cut(s) 467, 548, 591, 608, 821
Sse9I AATT 3 cut(s) 19, 70, 776
SsiI CCGC 3 cut(s) 204, 377, 504
SspMI CTAG 1 cut(s) 672
StyD4I CCNGG 4 cut(s) 249, 490, 796, 886
TaqI TCGA 4 cut(s) 262, 320, 333, 410
TasI AATT 3 cut(s) 19, 70, 776
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 4 cut(s) 233, 577, 682, 781
Tru1I TTAA 2 cut(s) 441, 609
Tru9I TTAA 2 cut(s) 441, 609
TseFI GTSAC 1 cut(s) 459
TseI GCWGC 3 cut(s) 289, 311, 932
Tsp45I GTSAC 1 cut(s) 459
TspDTI ATGAA 3 cut(s) 17, 857, 868
TspGWI ACGGA 1 cut(s) 338
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 487, 507
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.