Rh4DG169700

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007361
Physical Location & Seq
Forward (+)
33895670 .. 33902174
6505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG169700.1

Sequence Viewer

Length: 843 bp
ATGAACTTGGGCAACCCCAATAACCCAAATGCTCCCTCACCATCACCTCAACTCAACCTCCAACAAGCCTTCACCTCTCTCCAAACCCACTGCTCTGGTCTCCTCCACCACCTCTCTCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCATCTCCAATCAACTCTCTCCAACCTCCAAAACCAAGCCAAACACGCATTCCATGCCGGGTTTTCGGGCTTTGATTCCTCCAAGCAGAACCCAGTTTGGGCTCGAATTGCTGTAAACAACAAAACCCAGTTCCCGAATTCTAGTGGCGATGGGCCCGGAGCTGCCATGTCGACCGAGGCCATCGAGGAGCGGCTGGCCGGCGTGCCGGTCTATGCTCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGTGGGAAGAGTCTTGGATTGTTTTGTTTCAAGAAGGAGGACGCAGAGTCACTGCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTTGTGGCTGTGGCTTTGAATAAGGTTTTTCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGATTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAGGAGAAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGTCGAGGAGCTTGATTCTAAGAAGCCAAAGCAAGAGCTATCGTCCAGTATTTTTCAGGAAGGAGGATTTAGAAAACTCACTATCAAGAGCTTCTCGTGAGCAGAATCAATTAAATCCTTTACTTAGACCAGGGGACATTCAGGTATGGATTTCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

280

Amino Acids

30.51

Weight (kDa)

9.25

Isoelectric Point (pI)

56.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 58 - 276 7.6e-73 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 670
AccBSI CCGCTC 1 cut(s) 349
AccI GTMKAC 1 cut(s) 329
AciI CCGC 3 cut(s) 349, 386, 513
AclWI GGATC 1 cut(s) 652
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 295, 395
AcuI CTGAAG 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 256
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 4 cut(s) 133, 451, 541, 837
AhdI GACNNNNNGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 811
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 5 cut(s) 320, 556, 693, 720, 773
AluI AGCT 5 cut(s) 320, 556, 693, 720, 773
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 1 cut(s) 652
AoxI GGCC 4 cut(s) 311, 336, 354, 573
ApaI GGGCCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 2 cut(s) 295, 395
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 4 cut(s) 311, 312, 496, 516
AsuC2I CCSGG 3 cut(s) 217, 315, 501
AsuHPI GGTGA 3 cut(s) 30, 36, 64
AvaII GGWCC 2 cut(s) 496, 516
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 2 cut(s) 262, 315
BauI CACGAG 1 cut(s) 777
BbsI GAAGAC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 307
BccI CCATC 5 cut(s) 49, 168, 302, 347, 560
BciT130I CCWGG 1 cut(s) 813
BcnI CCSGG 3 cut(s) 217, 315, 501
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 2 cut(s) 300, 841
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 3 cut(s) 321, 350, 387
BlsI GCNGC 3 cut(s) 322, 351, 388
Bme1390I CCNGG 4 cut(s) 217, 315, 501, 813
Bme18I GGWCC 2 cut(s) 496, 516
BmeRI GACNNNNNGTC 1 cut(s) 466
BmgT120I GGNCC 4 cut(s) 311, 312, 496, 516
BmiI GGNNCC 4 cut(s) 313, 498, 517, 672
BmrFI CCNGG 4 cut(s) 217, 315, 501, 813
BmrI ACTGGG 2 cut(s) 245, 280
BmuI ACTGGG 2 cut(s) 245, 280
BpiI GAAGAC 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 390
BpuEI CTTGAG 3 cut(s) 497, 542, 585
BpuMI CCSGG 3 cut(s) 217, 315, 501
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 333, 499, 812
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse118I RCCGGY 2 cut(s) 356, 364
Bse1I ACTGG 5 cut(s) 251, 286, 674, 682, 728
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 1 cut(s) 813
BseDI CCNNGG 3 cut(s) 333, 499, 812
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 3 cut(s) 368, 381, 582
BseNI ACTGG 5 cut(s) 251, 286, 674, 682, 728
BseRI GAGGAG 3 cut(s) 92, 359, 703
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 1 cut(s) 307
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 4 cut(s) 313, 338, 356, 575
BshNI GGYRCC 1 cut(s) 670
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 5 cut(s) 216, 315, 357, 365, 501
BslFI GGGAC 1 cut(s) 830
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 830
BsmI GAATGC 3 cut(s) 206, 510, 616
BsnI GGCC 4 cut(s) 313, 338, 356, 575
Bso31I GGTCTC 1 cut(s) 104
Bsp120I GGGCCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 262, 315
Bsp143I GATC 1 cut(s) 644
BspACI CCGC 3 cut(s) 349, 386, 513
BspANI GGCC 4 cut(s) 313, 338, 356, 575
BspCNI CTCAG 3 cut(s) 369, 382, 583
BspLI GGNNCC 4 cut(s) 313, 498, 517, 672
BspPI GGATC 1 cut(s) 652
BspT107I GGYRCC 1 cut(s) 670
BspTI CTTAAG 1 cut(s) 617
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 2 cut(s) 356, 364
BsrI ACTGG 5 cut(s) 251, 286, 674, 682, 728
BssAI RCCGGY 2 cut(s) 356, 364
BssECI CCNNGG 3 cut(s) 333, 499, 812
BssMI GATC 1 cut(s) 644
BssSI CACGAG 1 cut(s) 777
Bst2BI CACGAG 1 cut(s) 777
Bst2UI CCWGG 1 cut(s) 813
Bst6I CTCTTC 2 cut(s) 134, 422
BstAFI CTTAAG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 354, 358, 362
BstDEI CTNAG 5 cut(s) 377, 390, 591, 701, 806
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 1 cut(s) 647
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 644
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 3 cut(s) 203, 212, 266
BstNI CCWGG 1 cut(s) 813
BstSCI CCNGG 4 cut(s) 215, 313, 499, 811
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 1 cut(s) 307
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 1 cut(s) 644
BstXI CCANNNNNNTGG 2 cut(s) 95, 526
BstYI RGATCY 1 cut(s) 644
BsuRI GGCC 4 cut(s) 313, 338, 356, 575
BtgZI GCGATG 1 cut(s) 321
BtsI GCAGTG 2 cut(s) 88, 470
BtsIMutI CAGTG 2 cut(s) 88, 470
Cac8I GCNNGC 3 cut(s) 354, 358, 362
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 2 cut(s) 356, 364
Cfr13I GGNCC 4 cut(s) 311, 312, 496, 516
CseI GACGC 1 cut(s) 470
CviAII CATG 2 cut(s) 212, 325
DdeI CTNAG 5 cut(s) 377, 390, 591, 701, 806
DpnI GATC 1 cut(s) 646
DpnII GATC 1 cut(s) 644
DriI GACNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 134, 422
Eam1105I GACNNNNNGTC 1 cut(s) 466
EarI CTCTTC 2 cut(s) 134, 422
Eco24I GRGCYC 2 cut(s) 262, 315
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 2 cut(s) 496, 516
Eco57I CTGAAG 1 cut(s) 562
EcoRI GAATTC 2 cut(s) 295, 395
EcoRII CCWGG 1 cut(s) 811
EcoT38I GRGCYC 2 cut(s) 262, 315
FaeI CATG 2 cut(s) 215, 328
FaiI YATR 6 cut(s) 213, 326, 372, 483, 494, 829
FaqI GGGAC 1 cut(s) 830
FatI CATG 2 cut(s) 211, 324
FauI CCCGC 1 cut(s) 506
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 3 cut(s) 321, 350, 387
FriOI GRGCYC 2 cut(s) 262, 315
Fsp4HI GCNGC 3 cut(s) 321, 350, 387
FspBI CTAG 2 cut(s) 300, 841
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 321, 350, 387
HaeIII GGCC 4 cut(s) 313, 338, 356, 575
HapII CCGG 5 cut(s) 216, 315, 357, 365, 501
HgaI GACGC 1 cut(s) 470
HhaI GCGC 1 cut(s) 616
Hin1II CATG 2 cut(s) 215, 328
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 7 cut(s) 233, 430, 467, 586, 593, 697, 787
HpaII CCGG 5 cut(s) 216, 315, 357, 365, 501
HphI GGTGA 3 cut(s) 30, 36, 64
Hpy166II GTNNAC 2 cut(s) 274, 330
Hpy188I TCNGA 2 cut(s) 378, 652
Hpy188III TCNNGA 7 cut(s) 292, 451, 590, 607, 739, 768, 779
Hpy8I GTNNAC 2 cut(s) 274, 330
HpyAV CCTTC 8 cut(s) 79, 153, 448, 586, 619, 625, 631, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 212, 266
HpyF3I CTNAG 5 cut(s) 377, 390, 591, 701, 806
Hsp92II CATG 2 cut(s) 215, 328
HspAI GCGC 1 cut(s) 614
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 1 cut(s) 644
LmnI GCTCC 4 cut(s) 37, 317, 346, 690
Lsp1109I GCAGC 1 cut(s) 307
MaeI CTAG 2 cut(s) 300, 841
MaeIII GTNAC 1 cut(s) 468
MalI GATC 1 cut(s) 646
MbiI CCGCTC 1 cut(s) 349
MboI GATC 1 cut(s) 644
MboII GAAGA 5 cut(s) 121, 138, 439, 588, 639
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 2 cut(s) 262, 315
MluCI AATT 4 cut(s) 264, 295, 395, 791
MlyI GAGTC 3 cut(s) 439, 476, 602
MmeI TCCRAC 2 cut(s) 85, 204
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 2 cut(s) 618, 794
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 5 cut(s) 216, 315, 357, 365, 501
MspR9I CCNGG 4 cut(s) 217, 315, 501, 813
Mva1269I GAATGC 3 cut(s) 206, 510, 616
MvaI CCWGG 1 cut(s) 813
MwoI GCNNNNNNNGC 3 cut(s) 203, 212, 266
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 3 cut(s) 217, 315, 501
NdeII GATC 1 cut(s) 644
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 2 cut(s) 215, 328
NlaIV GGNNCC 4 cut(s) 313, 498, 517, 672
NmuCI GTSAC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PctI GAATGC 3 cut(s) 206, 510, 616
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 4 cut(s) 233, 586, 697, 787
PkrI GCNGC 3 cut(s) 322, 351, 388
PleI GAGTC 3 cut(s) 438, 475, 601
PpsI GAGTC 3 cut(s) 438, 475, 601
Psp6I CCWGG 1 cut(s) 811
PspGI CCWGG 1 cut(s) 811
PspN4I GGNNCC 4 cut(s) 313, 498, 517, 672
PspOMI GGGCCC 1 cut(s) 311
PspPI GGNCC 4 cut(s) 311, 312, 496, 516
PsuI RGATCY 1 cut(s) 644
SalI GTCGAC 1 cut(s) 328
SaqAI TTAA 2 cut(s) 618, 794
SatI GCNGC 3 cut(s) 321, 350, 387
Sau3AI GATC 1 cut(s) 644
Sau96I GGNCC 4 cut(s) 311, 312, 496, 516
SchI GAGTC 3 cut(s) 439, 476, 602
ScrFI CCNGG 4 cut(s) 217, 315, 501, 813
SduI GDGCHC 2 cut(s) 262, 315
SinI GGWCC 2 cut(s) 496, 516
SmlI CTYRAG 4 cut(s) 476, 557, 600, 617
SmoI CTYRAG 4 cut(s) 476, 557, 600, 617
Sse9I AATT 4 cut(s) 264, 295, 395, 791
SsiI CCGC 3 cut(s) 349, 386, 513
SspMI CTAG 2 cut(s) 300, 841
StyD4I CCNGG 4 cut(s) 215, 313, 499, 811
TaqI TCGA 5 cut(s) 262, 329, 342, 419, 686
TaqII GACCGA 1 cut(s) 347
TasI AATT 4 cut(s) 264, 295, 395, 791
TauI GCSGC 2 cut(s) 352, 389
TfiI GAWTC 4 cut(s) 233, 586, 697, 787
Tru1I TTAA 2 cut(s) 618, 794
Tru9I TTAA 2 cut(s) 618, 794
TscAI CASTG 2 cut(s) 95, 477
TseFI GTSAC 1 cut(s) 468
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 468
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 95, 477
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 2 cut(s) 496, 516
XapI RAATTY 2 cut(s) 295, 395
XmiI GTMKAC 1 cut(s) 329
XspI CTAG 2 cut(s) 300, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.