Rh4BG173400

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007361
Physical Location & Seq
Forward (+)
30961069 .. 30967541
6473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG173400.1

Sequence Viewer

Length: 717 bp
ATGAACTTGGGCAACCGCAATAACCCAAATGCTCCCTCACCACCACCTCAACTCAACCTCCAACAAGCCTTCACCTCCCTCCGAACCCACTGCTCTGGTTTCCTCCACCACCTCTCTCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCACCTCCAATCAACTCTCTCCAACCTCCAAAACCAAGCCAAAAACGCATTCCATGCCGGGTTTTCGGGCTTTGATTCCTCCAAGCAGAACGCAGTTTGGGCTCGAATTGCTGTAAACAACAAAACCCAGTTCCCGAATTCTAGTGGCGATGGGCCCGGAGCTGCCATGTCGACTGAGGCCATCGAGGAGCGGCTGGCCGGCGTGCCGGTTTATGCCCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGCGGGAAGAGTCTTGGGTTGTTTTGTTTCAAGAAGGAGGACGCGGAGTCACTGCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTTGTGGCTGTGGCTTTGAATAAGGTTTTCCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGGTTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAGGAGAAGGAGAAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGGTTCTCCTCTTAGCTTTTCTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

238

Amino Acids

25.62

Weight (kDa)

8.89

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 58 - 234 6.4e-54 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 676
AccBSI CCGCTC 2 cut(s) 349, 423
AccI GTMKAC 1 cut(s) 329
AccII CGCG 1 cut(s) 464
AciI CCGC 6 cut(s) 16, 349, 386, 423, 464, 513
AclWI GGATC 1 cut(s) 658
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 295, 395
AcuI CTGAAG 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 559
AflII CTTAAG 1 cut(s) 617
AgsI TTSAA 3 cut(s) 133, 451, 541
AhdI GACNNNNNGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 3 cut(s) 320, 556, 704
AluI AGCT 3 cut(s) 320, 556, 704
AlwI GGATC 1 cut(s) 658
AoxI GGCC 4 cut(s) 311, 336, 354, 573
ApaI GGGCCC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 2 cut(s) 295, 395
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 4 cut(s) 311, 312, 496, 516
AsuC2I CCSGG 3 cut(s) 217, 315, 501
AsuHPI GGTGA 2 cut(s) 30, 64
AvaII GGWCC 2 cut(s) 496, 516
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 676
BanII GRGCYC 2 cut(s) 262, 315
BbsI GAAGAC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 307
BccI CCATC 3 cut(s) 302, 347, 560
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 1 cut(s) 689
BcnI CCSGG 3 cut(s) 217, 315, 501
BfaI CTAG 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 3 cut(s) 321, 350, 387
BlsI GCNGC 3 cut(s) 322, 351, 388
Bme1390I CCNGG 4 cut(s) 217, 315, 501, 689
Bme18I GGWCC 2 cut(s) 496, 516
BmeRI GACNNNNNGTC 1 cut(s) 466
BmgT120I GGNCC 4 cut(s) 311, 312, 496, 516
BmiI GGNNCC 5 cut(s) 313, 498, 517, 588, 678
BmrFI CCNGG 4 cut(s) 217, 315, 501, 689
BmrI ACTGGG 1 cut(s) 280
BmuI ACTGGG 1 cut(s) 280
BpiI GAAGAC 1 cut(s) 588
Bpu10I CCTNAGC 2 cut(s) 377, 390
BpuEI CTTGAG 3 cut(s) 497, 542, 585
BpuMI CCSGG 3 cut(s) 217, 315, 501
BsaJI CCNNGG 1 cut(s) 499
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse118I RCCGGY 2 cut(s) 356, 364
Bse1I ACTGG 2 cut(s) 286, 680
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 1 cut(s) 499
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 4 cut(s) 324, 368, 381, 582
BseNI ACTGG 2 cut(s) 286, 680
BseRI GAGGAG 2 cut(s) 359, 686
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 4 cut(s) 313, 338, 356, 575
BshNI GGYRCC 1 cut(s) 676
BsiSI CCGG 5 cut(s) 216, 315, 357, 365, 501
BslI CCNNNNNNNGG 1 cut(s) 559
BsmI GAATGC 3 cut(s) 206, 510, 616
BsnI GGCC 4 cut(s) 313, 338, 356, 575
Bsp120I GGGCCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 262, 315
Bsp143I GATC 1 cut(s) 650
BspACI CCGC 6 cut(s) 16, 349, 386, 423, 464, 513
BspANI GGCC 4 cut(s) 313, 338, 356, 575
BspCNI CTCAG 4 cut(s) 325, 369, 382, 583
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 5 cut(s) 313, 498, 517, 588, 678
BspPI GGATC 1 cut(s) 658
BspT107I GGYRCC 1 cut(s) 676
BspTI CTTAAG 1 cut(s) 617
BsrBI CCGCTC 2 cut(s) 349, 423
BsrDI GCAATG 1 cut(s) 388
BsrFI RCCGGY 2 cut(s) 356, 364
BsrI ACTGG 2 cut(s) 286, 680
BssAI RCCGGY 2 cut(s) 356, 364
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 689
Bst6I CTCTTC 2 cut(s) 134, 422
BstAFI CTTAAG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 354, 358, 362
BstDEI CTNAG 5 cut(s) 333, 377, 390, 591, 700
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 1 cut(s) 653
BstMBI GATC 1 cut(s) 650
BstMWI GCNNNNNNNGC 4 cut(s) 203, 212, 257, 266
BstNI CCWGG 1 cut(s) 689
BstSCI CCNGG 4 cut(s) 215, 313, 499, 687
BstSLI GKGCMC 1 cut(s) 315
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 307
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 1 cut(s) 650
BstXI CCANNNNNNTGG 2 cut(s) 95, 526
BstYI RGATCY 1 cut(s) 650
BsuRI GGCC 4 cut(s) 313, 338, 356, 575
BtgZI GCGATG 1 cut(s) 321
BtsI GCAGTG 2 cut(s) 88, 470
BtsIMutI CAGTG 2 cut(s) 88, 470
Cac8I GCNNGC 3 cut(s) 354, 358, 362
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 2 cut(s) 356, 364
Cfr13I GGNCC 4 cut(s) 311, 312, 496, 516
CseI GACGC 1 cut(s) 470
CviAII CATG 2 cut(s) 212, 325
DdeI CTNAG 5 cut(s) 333, 377, 390, 591, 700
DpnI GATC 1 cut(s) 652
DpnII GATC 1 cut(s) 650
DriI GACNNNNNGTC 1 cut(s) 466
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 134, 422
Eam1105I GACNNNNNGTC 1 cut(s) 466
EarI CTCTTC 2 cut(s) 134, 422
Eco24I GRGCYC 2 cut(s) 262, 315
Eco47I GGWCC 2 cut(s) 496, 516
Eco57I CTGAAG 1 cut(s) 562
EcoRI GAATTC 2 cut(s) 295, 395
EcoRII CCWGG 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 262, 315
FaeI CATG 2 cut(s) 215, 328
FaiI YATR 5 cut(s) 213, 326, 372, 483, 494
FatI CATG 2 cut(s) 211, 324
FauI CCCGC 2 cut(s) 416, 506
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 3 cut(s) 321, 350, 387
FriOI GRGCYC 2 cut(s) 262, 315
Fsp4HI GCNGC 3 cut(s) 321, 350, 387
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 321, 350, 387
HaeIII GGCC 4 cut(s) 313, 338, 356, 575
HapII CCGG 5 cut(s) 216, 315, 357, 365, 501
HgaI GACGC 1 cut(s) 470
HhaI GCGC 1 cut(s) 616
Hin1II CATG 2 cut(s) 215, 328
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 4 cut(s) 233, 430, 467, 593
HpaII CCGG 5 cut(s) 216, 315, 357, 365, 501
HphI GGTGA 2 cut(s) 30, 64
Hpy166II GTNNAC 2 cut(s) 274, 330
Hpy188I TCNGA 2 cut(s) 83, 658
Hpy188III TCNNGA 4 cut(s) 292, 451, 590, 607
Hpy8I GTNNAC 2 cut(s) 274, 330
HpyAV CCTTC 8 cut(s) 79, 153, 448, 586, 619, 625, 631, 637
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 212, 257, 266
HpyF3I CTNAG 5 cut(s) 333, 377, 390, 591, 700
Hsp92II CATG 2 cut(s) 215, 328
HspAI GCGC 1 cut(s) 614
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Kzo9I GATC 1 cut(s) 650
LmnI GCTCC 3 cut(s) 37, 317, 346
Lsp1109I GCAGC 1 cut(s) 307
MaeI CTAG 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 468
MalI GATC 1 cut(s) 652
MbiI CCGCTC 2 cut(s) 349, 423
MboI GATC 1 cut(s) 650
MboII GAAGA 5 cut(s) 121, 138, 439, 588, 645
MfeI CAATTG 1 cut(s) 712
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 262, 315
MluCI AATT 4 cut(s) 264, 295, 395, 712
MlyI GAGTC 3 cut(s) 439, 476, 602
MmeI TCCRAC 2 cut(s) 85, 204
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 1 cut(s) 618
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 5 cut(s) 216, 315, 357, 365, 501
MspR9I CCNGG 4 cut(s) 217, 315, 501, 689
MunI CAATTG 1 cut(s) 712
Mva1269I GAATGC 3 cut(s) 206, 510, 616
MvaI CCWGG 1 cut(s) 689
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 203, 212, 257, 266
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 3 cut(s) 217, 315, 501
NdeII GATC 1 cut(s) 650
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 2 cut(s) 215, 328
NlaIV GGNNCC 5 cut(s) 313, 498, 517, 588, 678
NmuCI GTSAC 1 cut(s) 468
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PctI GAATGC 3 cut(s) 206, 510, 616
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 233
PkrI GCNGC 3 cut(s) 322, 351, 388
PleI GAGTC 3 cut(s) 438, 475, 601
PpsI GAGTC 3 cut(s) 438, 475, 601
Psp6I CCWGG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 5 cut(s) 313, 498, 517, 588, 678
PspOMI GGGCCC 1 cut(s) 311
PspPI GGNCC 4 cut(s) 311, 312, 496, 516
PsuI RGATCY 1 cut(s) 650
SalI GTCGAC 1 cut(s) 328
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 3 cut(s) 321, 350, 387
Sau3AI GATC 1 cut(s) 650
Sau96I GGNCC 4 cut(s) 311, 312, 496, 516
SchI GAGTC 3 cut(s) 439, 476, 602
ScrFI CCNGG 4 cut(s) 217, 315, 501, 689
SduI GDGCHC 2 cut(s) 262, 315
SinI GGWCC 2 cut(s) 496, 516
SmlI CTYRAG 4 cut(s) 476, 557, 600, 617
SmoI CTYRAG 4 cut(s) 476, 557, 600, 617
Sse9I AATT 4 cut(s) 264, 295, 395, 712
SsiI CCGC 6 cut(s) 16, 349, 386, 423, 464, 513
SspMI CTAG 1 cut(s) 300
StyD4I CCNGG 4 cut(s) 215, 313, 499, 687
TaqI TCGA 4 cut(s) 262, 329, 342, 419
TasI AATT 4 cut(s) 264, 295, 395, 712
TauI GCSGC 2 cut(s) 352, 389
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 2 cut(s) 95, 477
TseFI GTSAC 1 cut(s) 468
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 468
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 95, 477
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 2 cut(s) 496, 516
XapI RAATTY 2 cut(s) 295, 395
XmiI GTMKAC 1 cut(s) 329
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.