Rh4DG169500

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007361
Physical Location & Seq
Forward (+)
33707358 .. 33709680
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG169500.1

Sequence Viewer

Length: 387 bp
ATGTCGACCGAGGCCATCGAGGAGCGGCTGGCTGGCGTGCCGGTCTATGCTCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGCGGGAAGAGTCTTGGGTTGTTTTGTTTCAAGAAGGAGGACGCGGAGTCACTGCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTTGTGGCTGTGGCTTTGAATAAGGTTTTTCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGATTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAGGAGAAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGGTTCTCCTCTTAGCTTTTCTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

128

Amino Acids

13.76

Weight (kDa)

5.87

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 1 - 124 1.1e-49 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 346
AccBSI CCGCTC 2 cut(s) 25, 99
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 140
AciI CCGC 5 cut(s) 25, 62, 99, 140, 189
AclWI GGATC 1 cut(s) 328
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 238
AflII CTTAAG 1 cut(s) 293
AgsI TTSAA 2 cut(s) 127, 217
AhdI GACNNNNNGTC 1 cut(s) 142
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 2 cut(s) 232, 374
AluI AGCT 2 cut(s) 232, 374
AlwI GGATC 1 cut(s) 328
AoxI GGCC 2 cut(s) 12, 249
ApoI RAATTY 1 cut(s) 71
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 2 cut(s) 172, 192
AsuC2I CCSGG 1 cut(s) 177
AvaII GGWCC 2 cut(s) 172, 192
BanI GGYRCC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 264
BbvCI CCTCAGC 1 cut(s) 66
BccI CCATC 2 cut(s) 23, 236
BciT130I CCWGG 1 cut(s) 359
BcnI CCSGG 1 cut(s) 177
BfrI CTTAAG 1 cut(s) 293
BisI GCNGC 2 cut(s) 26, 63
BlsI GCNGC 2 cut(s) 27, 64
Bme1390I CCNGG 2 cut(s) 177, 359
Bme18I GGWCC 2 cut(s) 172, 192
BmeRI GACNNNNNGTC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 172, 192
BmiI GGNNCC 3 cut(s) 174, 193, 348
BmrFI CCNGG 2 cut(s) 177, 359
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 173, 218, 261
BpuMI CCSGG 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 9, 175
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 64
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 2 cut(s) 9, 175
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 3 cut(s) 44, 57, 258
BseNI ACTGG 1 cut(s) 350
BseRI GAGGAG 2 cut(s) 35, 356
Bsh1236I CGCG 1 cut(s) 140
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 14, 251
BshNI GGYRCC 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 41, 177
BsmI GAATGC 2 cut(s) 186, 292
BsnI GGCC 2 cut(s) 14, 251
Bsp143I GATC 1 cut(s) 320
BspACI CCGC 5 cut(s) 25, 62, 99, 140, 189
BspANI GGCC 2 cut(s) 14, 251
BspCNI CTCAG 3 cut(s) 45, 58, 259
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 3 cut(s) 174, 193, 348
BspPI GGATC 1 cut(s) 328
BspT107I GGYRCC 1 cut(s) 346
BspTI CTTAAG 1 cut(s) 293
BsrBI CCGCTC 2 cut(s) 25, 99
BsrDI GCAATG 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 1 cut(s) 350
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 9, 175
BssMI GATC 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 293
BstC8I GCNNGC 3 cut(s) 30, 34, 38
BstDEI CTNAG 4 cut(s) 53, 66, 267, 370
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 1 cut(s) 323
BstMBI GATC 1 cut(s) 320
BstMCI CGRYCG 1 cut(s) 9
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 2 cut(s) 175, 357
BstUI CGCG 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 2 cut(s) 14, 251
BtsI GCAGTG 1 cut(s) 146
BtsIMutI CAGTG 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 30, 34, 38
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 172, 192
CseI GACGC 1 cut(s) 146
DdeI CTNAG 4 cut(s) 53, 66, 267, 370
DpnI GATC 1 cut(s) 322
DpnII GATC 1 cut(s) 320
DriI GACNNNNNGTC 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 98
Eam1105I GACNNNNNGTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 98
Eco47I GGWCC 2 cut(s) 172, 192
Eco57I CTGAAG 1 cut(s) 238
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 357
FaiI YATR 3 cut(s) 48, 159, 170
FauI CCCGC 2 cut(s) 92, 182
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 26, 63
Fsp4HI GCNGC 2 cut(s) 26, 63
GlaI GCGC 1 cut(s) 291
GluI GCNGC 2 cut(s) 26, 63
HaeIII GGCC 2 cut(s) 14, 251
HapII CCGG 2 cut(s) 41, 177
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 292
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 106, 143, 262, 269
HpaII CCGG 2 cut(s) 41, 177
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 54, 328
Hpy188III TCNNGA 3 cut(s) 127, 266, 283
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 5 cut(s) 124, 262, 295, 301, 307
HpyF3I CTNAG 4 cut(s) 53, 66, 267, 370
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 1 cut(s) 320
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 9 cut(s) 14, 18, 54, 190, 241, 279, 344, 363, 371
MaeIII GTNAC 1 cut(s) 144
MalI GATC 1 cut(s) 322
MbiI CCGCTC 2 cut(s) 25, 99
MboI GATC 1 cut(s) 320
MboII GAAGA 3 cut(s) 115, 264, 315
MfeI CAATTG 1 cut(s) 382
MflI RGATCY 1 cut(s) 320
MluCI AATT 2 cut(s) 71, 382
MlyI GAGTC 3 cut(s) 115, 152, 278
MnlI CCTC 5 cut(s) 4, 13, 61, 127, 377
MseI TTAA 1 cut(s) 294
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 2 cut(s) 41, 177
MspR9I CCNGG 2 cut(s) 177, 359
MunI CAATTG 1 cut(s) 382
Mva1269I GAATGC 2 cut(s) 186, 292
MvaI CCWGG 1 cut(s) 359
MvnI CGCG 1 cut(s) 140
NciI CCSGG 1 cut(s) 177
NdeII GATC 1 cut(s) 320
NlaIV GGNNCC 3 cut(s) 174, 193, 348
NmuCI GTSAC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PctI GAATGC 2 cut(s) 186, 292
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 2 cut(s) 27, 64
PleI GAGTC 3 cut(s) 114, 151, 277
PpsI GAGTC 3 cut(s) 114, 151, 277
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 3 cut(s) 174, 193, 348
PspPI GGNCC 2 cut(s) 172, 192
PsuI RGATCY 1 cut(s) 320
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 2 cut(s) 26, 63
Sau3AI GATC 1 cut(s) 320
Sau96I GGNCC 2 cut(s) 172, 192
SchI GAGTC 3 cut(s) 115, 152, 278
ScrFI CCNGG 2 cut(s) 177, 359
SetI ASST 6 cut(s) 225, 234, 240, 260, 363, 376
SinI GGWCC 2 cut(s) 172, 192
SmlI CTYRAG 4 cut(s) 152, 233, 276, 293
SmoI CTYRAG 4 cut(s) 152, 233, 276, 293
Sse9I AATT 2 cut(s) 71, 382
SsiI CCGC 5 cut(s) 25, 62, 99, 140, 189
StyD4I CCNGG 2 cut(s) 175, 357
TaqI TCGA 3 cut(s) 5, 18, 95
TaqII GACCGA 1 cut(s) 23
TasI AATT 2 cut(s) 71, 382
TauI GCSGC 2 cut(s) 28, 65
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 153
TseFI GTSAC 1 cut(s) 144
Tsp45I GTSAC 1 cut(s) 144
TspRI CASTG 1 cut(s) 153
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 2 cut(s) 172, 192
XapI RAATTY 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.