MD04G1021300.v1.1

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007361
Physical Location & Seq
Reverse (-)
2534071 .. 2538866
4796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1021300.v1.1.491

Sequence Viewer

Length: 960 bp
ATGAACTTGGGAAACTCCAATAACCAGAAAACTTCCTCACCACCGACACACAATCCGCCCCAACTCAGCCCCCAACAGGCCTTCACCAACCTCCAAAACCATTGCTCCGGTTTCCTCCACCACCTCTCCCACCTTCCCCTCTTCAACCCGAACCCTTCTTCCCTCCAAACCCACCTCCAATCCACCTTCTCCAACCTCCAGAATCAAGCAAAACATGCATTCGATTCCAGGTTTTCGAGCTTCAATCCGGTGCATTCTTCTGGCAAGAACCCAGTTTGGGGTCGAATTGCGGTGAGTAACAAAACCCAGGTCGCCAATTCCCCTGGTGGCGGGTCTAAAGCCGCCATGTCCACCGAGGACATCGAAGAGCGGCTTGCCGGCGTGCCGGTTTATGCTCTCAGTAATGCCTCGGAGGAATTCGTGTTGGTTTCTGGTGCTTCCACTGGGAAGTCTCTGGGATTGTTTTGTTTCAAGAAAGAGGATGCGGATGCACTTCTTGAGCATATTAGGATTATGGACCCCGGAATGCGAAGCGGGTCGAAAGTGGTGGCTGTGGCTTTAAATAAGGTTTTTCAGCTCAAGGTCGATGGAGTAGCCTTCAGGTTGATTCCTGAGTATTCTCAAGTCAAGAATGCGCTTAAGGAGAGAGAAAAGGTTGGAACTTCTGATGATGATGGCTTTCCTGGTGTACCAGTTTTCCAGTCAAGGAGCTTGGTTCTGAAAAGCCAAAGCAAGAGCTATCGTCCAGTATTTTTCAGGAAGGAGGATTTGGAAAATTCACTATCAAGAGCTTCTCGTGACCAGAATCAATTAAATCCTGCCTTCAGACCAGGGGATGTTCAGGTTGCTGTTCTTGAGGAAGTCATAAAGGGGATGAAGGAGGGTTCTACATCAACGTGGGATGACGTCGTCTTCATCCCTCCTGGATTTGACATCTCTACTGATCCCAACAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

320

Amino Acids

34.86

Weight (kDa)

8.45

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 62 - 315 1.1e-88 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 909
AccBSI CCGCTC 1 cut(s) 370
AciI CCGC 7 cut(s) 56, 290, 330, 342, 370, 485, 534
AclWI GGATC 1 cut(s) 938
AcsI RAATTY 2 cut(s) 416, 775
AcuI CTGAAG 2 cut(s) 583, 808
AcyI GRCGYC 1 cut(s) 906
AfaI GTAC 1 cut(s) 690
AfiI CCNNNNNNNGG 4 cut(s) 76, 277, 278, 329
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 3 cut(s) 145, 244, 472
AjnI CCWGG 6 cut(s) 227, 306, 322, 682, 829, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 752, 784
AloI GAACNNNNNNTCC 2 cut(s) 143, 175
AluBI AGCT 5 cut(s) 240, 577, 711, 738, 791
AluI AGCT 5 cut(s) 240, 577, 711, 738, 791
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 1 cut(s) 938
AoxI GGCC 1 cut(s) 78
ApoI RAATTY 2 cut(s) 416, 775
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 1 cut(s) 517
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 3 cut(s) 30, 76, 304
AvaII GGWCC 1 cut(s) 517
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 904
BccI CCATC 2 cut(s) 581, 668
BciT130I CCWGG 6 cut(s) 229, 308, 324, 684, 831, 924
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 456
BfrI CTTAAG 1 cut(s) 638
BisI GCNGC 2 cut(s) 342, 371
BlsI GCNGC 2 cut(s) 343, 372
Bme1390I CCNGG 7 cut(s) 229, 308, 324, 522, 684, 831, 924
Bme18I GGWCC 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 7 cut(s) 229, 308, 324, 522, 684, 831, 924
BmrI ACTGGG 2 cut(s) 266, 453
BmsI GCATC 2 cut(s) 472, 478
BmuI ACTGGG 2 cut(s) 266, 453
BpiI GAAGAC 1 cut(s) 904
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 4 cut(s) 518, 563, 606, 875
BpuMI CCSGG 1 cut(s) 522
BsaHI GRCGYC 1 cut(s) 906
BsaJI CCNNGG 6 cut(s) 306, 322, 354, 408, 520, 830
BsaWI WCCGGW 2 cut(s) 107, 247
BsaXI ACNNNNNCTCC 4 cut(s) 89, 110, 119, 140
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 277, 278, 329
Bse118I RCCGGY 2 cut(s) 377, 385
Bse1I ACTGG 5 cut(s) 272, 448, 692, 700, 746
Bse3DI GCAATG 1 cut(s) 100
BseBI CCWGG 6 cut(s) 229, 308, 324, 684, 831, 924
BseDI CCNNGG 6 cut(s) 306, 322, 354, 408, 520, 830
BseGI GGATG 6 cut(s) 487, 493, 841, 879, 907, 915
BseLI CCNNNNNNNGG 4 cut(s) 76, 277, 278, 329
BseMI GCAATG 1 cut(s) 100
BseMII CTCAG 3 cut(s) 79, 412, 603
BseNI ACTGG 5 cut(s) 272, 448, 692, 700, 746
BshFI GGCC 1 cut(s) 80
BsiSI CCGG 5 cut(s) 108, 248, 378, 386, 522
BslI CCNNNNNNNGG 4 cut(s) 76, 277, 278, 329
BsmAI GTCTC 1 cut(s) 456
BsmI GAATGC 4 cut(s) 218, 253, 531, 637
BsnI GGCC 1 cut(s) 80
Bsp143I GATC 1 cut(s) 943
BspACI CCGC 7 cut(s) 56, 290, 330, 342, 370, 485, 534
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 3 cut(s) 78, 411, 604
BspLI GGNNCC 1 cut(s) 519
BspPI GGATC 1 cut(s) 938
BspQI GCTCTTC 1 cut(s) 360
BspTI CTTAAG 1 cut(s) 638
BsrBI CCGCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 2 cut(s) 377, 385
BsrI ACTGG 5 cut(s) 272, 448, 692, 700, 746
BssAI RCCGGY 2 cut(s) 377, 385
BssECI CCNNGG 6 cut(s) 306, 322, 354, 408, 520, 830
BssMI GATC 1 cut(s) 943
BssNI GRCGYC 1 cut(s) 906
BssSI CACGAG 1 cut(s) 795
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 6 cut(s) 229, 308, 324, 684, 831, 924
Bst6I CTCTTC 2 cut(s) 146, 360
BstACI GRCGYC 1 cut(s) 906
BstAFI CTTAAG 1 cut(s) 638
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 3 cut(s) 375, 379, 383
BstDEI CTNAG 3 cut(s) 65, 398, 612
BstF5I GGATG 6 cut(s) 487, 493, 841, 879, 907, 915
BstHHI GCGC 1 cut(s) 637
BstKTI GATC 1 cut(s) 946
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 1 cut(s) 943
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 6 cut(s) 229, 308, 324, 684, 831, 924
BstNSI RCATGY 1 cut(s) 218
BstSCI CCNGG 7 cut(s) 227, 306, 322, 520, 682, 829, 922
BstV2I GAAGAC 1 cut(s) 904
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 6 cut(s) 487, 493, 841, 879, 907, 915
BtsIMutI CAGTG 1 cut(s) 441
Cac8I GCNNGC 3 cut(s) 375, 379, 383
CfoI GCGC 1 cut(s) 637
Cfr10I RCCGGY 2 cut(s) 377, 385
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 1 cut(s) 689
CviAII CATG 2 cut(s) 215, 346
CviQI GTAC 1 cut(s) 689
DdeI CTNAG 3 cut(s) 65, 398, 612
DpnI GATC 1 cut(s) 945
DpnII GATC 1 cut(s) 943
DraI TTTAAA 1 cut(s) 561
Eam1104I CTCTTC 2 cut(s) 146, 360
EarI CTCTTC 2 cut(s) 146, 360
EciI GGCGGA 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 517
Eco57I CTGAAG 2 cut(s) 583, 808
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 6 cut(s) 227, 306, 322, 682, 829, 922
EcoT22I ATGCAT 1 cut(s) 220
FaeI CATG 2 cut(s) 218, 349
FaiI YATR 6 cut(s) 216, 347, 393, 504, 515, 866
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 2 cut(s) 214, 345
FauI CCCGC 2 cut(s) 323, 527
Fnu4HI GCNGC 2 cut(s) 342, 371
FokI GGATG 6 cut(s) 494, 500, 848, 886, 902, 914
Fsp4HI GCNGC 2 cut(s) 342, 371
GlaI GCGC 1 cut(s) 636
GluI GCNGC 2 cut(s) 342, 371
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 1 cut(s) 80
HapII CCGG 5 cut(s) 108, 248, 378, 386, 522
HhaI GCGC 1 cut(s) 637
Hin1I GRCGYC 1 cut(s) 906
Hin1II CATG 2 cut(s) 218, 349
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HinfI GANTC 4 cut(s) 202, 224, 607, 805
HpaII CCGG 5 cut(s) 108, 248, 378, 386, 522
HphI GGTGA 3 cut(s) 30, 76, 304
Hpy166II GTNNAC 2 cut(s) 351, 689
Hpy188I TCNGA 4 cut(s) 412, 667, 720, 827
Hpy188III TCNNGA 9 cut(s) 199, 472, 497, 611, 628, 757, 786, 797, 854
Hpy8I GTNNAC 2 cut(s) 351, 689
Hpy99I CGWCG 1 cut(s) 911
HpyAV CCTTC 8 cut(s) 91, 143, 165, 196, 607, 754, 832, 871
HpyCH4IV ACGT 2 cut(s) 896, 906
HpyCH4V TGCA 3 cut(s) 218, 253, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 3 cut(s) 65, 398, 612
HpySE526I ACGT 2 cut(s) 896, 906
Hsp92I GRCGYC 1 cut(s) 906
Hsp92II CATG 2 cut(s) 218, 349
HspAI GCGC 1 cut(s) 635
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Kzo9I GATC 1 cut(s) 943
LguI GCTCTTC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 110, 708
LweI GCATC 2 cut(s) 472, 478
MaeII ACGT 2 cut(s) 896, 906
MaeIII GTNAC 2 cut(s) 296, 797
MalI GATC 1 cut(s) 945
MbiI CCGCTC 1 cut(s) 370
MboI GATC 1 cut(s) 943
MboII GAAGA 5 cut(s) 133, 150, 249, 377, 904
MluCI AATT 5 cut(s) 285, 316, 416, 775, 809
MmeI TCCRAC 2 cut(s) 216, 637
Mph1103I ATGCAT 1 cut(s) 220
MroNI GCCGGC 1 cut(s) 377
MseI TTAA 3 cut(s) 560, 639, 812
MslI CAYNNNNRTG 1 cut(s) 895
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 5 cut(s) 108, 248, 378, 386, 522
MspR9I CCNGG 7 cut(s) 229, 308, 324, 522, 684, 831, 924
Mva1269I GAATGC 4 cut(s) 218, 253, 531, 637
MvaI CCWGG 6 cut(s) 229, 308, 324, 684, 831, 924
MwoI GCNNNNNNNGC 1 cut(s) 215
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 1 cut(s) 522
NdeII GATC 1 cut(s) 943
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 2 cut(s) 218, 349
NlaIV GGNNCC 1 cut(s) 519
NmuCI GTSAC 1 cut(s) 797
NsiI ATGCAT 1 cut(s) 220
NspI RCATGY 1 cut(s) 218
PceI AGGCCT 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 360
PctI GAATGC 4 cut(s) 218, 253, 531, 637
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 4 cut(s) 202, 224, 607, 805
PflFI GACNNNGTC 1 cut(s) 908
PfoI TCCNGGA 1 cut(s) 922
PkrI GCNGC 2 cut(s) 343, 372
Psp6I CCWGG 6 cut(s) 227, 306, 322, 682, 829, 922
PspGI CCWGG 6 cut(s) 227, 306, 322, 682, 829, 922
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 908
RsaI GTAC 1 cut(s) 690
RsaNI GTAC 1 cut(s) 689
RseI CAYNNNNRTG 1 cut(s) 895
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 3 cut(s) 560, 639, 812
SatI GCNGC 2 cut(s) 342, 371
Sau3AI GATC 1 cut(s) 943
Sau96I GGNCC 1 cut(s) 517
ScrFI CCNGG 7 cut(s) 229, 308, 324, 522, 684, 831, 924
SfaNI GCATC 2 cut(s) 472, 478
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 895
SmlI CTYRAG 5 cut(s) 497, 578, 621, 638, 854
SmoI CTYRAG 5 cut(s) 497, 578, 621, 638, 854
Sse9I AATT 5 cut(s) 285, 316, 416, 775, 809
SseBI AGGCCT 1 cut(s) 80
SsiI CCGC 7 cut(s) 56, 290, 330, 342, 370, 485, 534
StuI AGGCCT 1 cut(s) 80
StyD4I CCNGG 7 cut(s) 227, 306, 322, 520, 682, 829, 922
TaiI ACGT 2 cut(s) 899, 909
TaqI TCGA 6 cut(s) 222, 236, 283, 363, 539, 585
TasI AATT 5 cut(s) 285, 316, 416, 775, 809
TauI GCSGC 2 cut(s) 344, 373
TfiI GAWTC 4 cut(s) 202, 224, 607, 805
Tru1I TTAA 3 cut(s) 560, 639, 812
Tru9I TTAA 3 cut(s) 560, 639, 812
TscAI CASTG 1 cut(s) 448
TseFI GTSAC 1 cut(s) 797
Tsp45I GTSAC 1 cut(s) 797
TspDTI ATGAA 3 cut(s) 17, 890, 904
TspRI CASTG 1 cut(s) 448
Tth111I GACNNNGTC 1 cut(s) 908
Vha464I CTTAAG 1 cut(s) 638
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 2 cut(s) 416, 775
XceI RCATGY 1 cut(s) 218
ZraI GACGTC 1 cut(s) 907
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.