RchiOBHm_Chr4g0412551

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007361
Physical Location & Seq
Forward (+)
36431262 .. 36437756
6495 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38322

Sequence Viewer

Length: 966 bp
ATGAACTTGGGCAACCCCAATAACCCAAATGCTCCCTCACCGCCACCCCCACCTCAACTCAACCTCCAACAAGCCTTCACCTCCCTCCAAACCCACTGCTCTGGTTTCCTCCACCACCTCTCTCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCACCTCCAATCATCCCTCTCCAACCTCCAAAACCAAGCCAAAAACGCATTCCATGCCGGGTTTTCGGGCTTTGATTCCTCCAAGCAGAACCCAGTTTGGGCTCGAATTGCTGTAAACAACAAAACCCAGTTCCCGAATTCTAGTGGCGATGGGCCCGGAGCTGCCATGTCGACCGAGGCCATCGAGGAGCGGCTGGCCGGCGTGCCGGTTTATGCTCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGCGGGAAGAGTCTTGGGTTGTTTTGTTTCAAGAAGGAGGACGCGGAGTCACTGCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTTGTGGCTGTGGCTTTGAATAAGGTTTTCCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGATTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAGGAGAAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGTCGAGGAGCTTGATTCTAAGAAGCCAAAGCAAGAGCTATCGTCCAGTATTTTTCAGGAAGGAGGATTTAGAAAACTCGCTATCAAGAGCTTCTCGTGAGCAGAATCAATTAAATCCTTTACTTAGACCAGGGGACATTCAGGTTGCTGTTCTCGAGGATGTAATAAAGGGGATGAAGGAGGGTTCTACATCGGTCTGGGATGACGTTGTTTTTATCCCTCCTGGATTTGATATCTCTACTGATCCCACCAAGCAACAGGCAGATGCTGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

321

Amino Acids

34.81

Weight (kDa)

7.79

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 59 - 311 1.3e-89 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 676
AccBSI CCGCTC 2 cut(s) 355, 429
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 470
AciI CCGC 6 cut(s) 41, 355, 392, 429, 470, 519
AclWI GGATC 2 cut(s) 658, 926
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 2 cut(s) 301, 401
AcuI CTGAAG 1 cut(s) 568
AfiI CCNNNNNNNGG 2 cut(s) 262, 565
AflII CTTAAG 1 cut(s) 623
AgsI TTSAA 3 cut(s) 139, 457, 547
AhdI GACNNNNNGTC 1 cut(s) 472
AjnI CCWGG 2 cut(s) 817, 910
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 5 cut(s) 326, 562, 699, 726, 779
AluI AGCT 5 cut(s) 326, 562, 699, 726, 779
AlwI GGATC 2 cut(s) 658, 926
AlwNI CAGNNNCTG 1 cut(s) 956
Ama87I CYCGRG 1 cut(s) 842
AoxI GGCC 4 cut(s) 317, 342, 360, 579
ApaI GGGCCC 1 cut(s) 321
ApeKI GCWGC 2 cut(s) 326, 956
ApoI RAATTY 2 cut(s) 301, 401
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 4 cut(s) 317, 318, 502, 522
AsuC2I CCSGG 3 cut(s) 223, 321, 507
AsuHPI GGTGA 2 cut(s) 30, 70
AvaI CYCGRG 1 cut(s) 842
AvaII GGWCC 2 cut(s) 502, 522
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 676
BanII GRGCYC 2 cut(s) 268, 321
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 594
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 2 cut(s) 313, 943
BccI CCATC 3 cut(s) 308, 353, 566
BciT130I CCWGG 2 cut(s) 819, 912
BcnI CCSGG 3 cut(s) 223, 321, 507
BfaI CTAG 1 cut(s) 306
BfrI CTTAAG 1 cut(s) 623
BisI GCNGC 4 cut(s) 327, 356, 393, 957
BlsI GCNGC 4 cut(s) 328, 357, 394, 958
Bme1390I CCNGG 5 cut(s) 223, 321, 507, 819, 912
Bme18I GGWCC 2 cut(s) 502, 522
BmeRI GACNNNNNGTC 1 cut(s) 472
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 4 cut(s) 317, 318, 502, 522
BmiI GGNNCC 4 cut(s) 319, 504, 523, 678
BmrFI CCNGG 5 cut(s) 223, 321, 507, 819, 912
BmrI ACTGGG 2 cut(s) 251, 286
BmsI GCATC 1 cut(s) 943
BmuI ACTGGG 2 cut(s) 251, 286
BpiI GAAGAC 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 3 cut(s) 503, 548, 591
BpuMI CCSGG 3 cut(s) 223, 321, 507
BsaJI CCNNGG 3 cut(s) 339, 505, 818
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 565
Bse118I RCCGGY 2 cut(s) 362, 370
Bse1I ACTGG 5 cut(s) 257, 292, 680, 688, 734
Bse3DI GCAATG 1 cut(s) 394
BseBI CCWGG 2 cut(s) 819, 912
BseDI CCNNGG 3 cut(s) 339, 505, 818
BseGI GGATG 4 cut(s) 176, 853, 867, 895
BseLI CCNNNNNNNGG 2 cut(s) 262, 565
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 3 cut(s) 374, 387, 588
BseNI ACTGG 5 cut(s) 257, 292, 680, 688, 734
BseRI GAGGAG 2 cut(s) 365, 709
BseSI GKGCMC 1 cut(s) 321
BseXI GCAGC 2 cut(s) 313, 943
Bsh1236I CGCG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 4 cut(s) 319, 344, 362, 581
BshNI GGYRCC 1 cut(s) 676
BsiEI CGRYCG 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 5 cut(s) 222, 321, 363, 371, 507
BslFI GGGAC 1 cut(s) 836
BslI CCNNNNNNNGG 2 cut(s) 262, 565
BsmFI GGGAC 1 cut(s) 836
BsmI GAATGC 3 cut(s) 212, 516, 622
BsnI GGCC 4 cut(s) 319, 344, 362, 581
BsoBI CYCGRG 1 cut(s) 842
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 268, 321
Bsp143I GATC 2 cut(s) 650, 931
BspACI CCGC 6 cut(s) 41, 355, 392, 429, 470, 519
BspANI GGCC 4 cut(s) 319, 344, 362, 581
BspCNI CTCAG 3 cut(s) 375, 388, 589
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 4 cut(s) 319, 504, 523, 678
BspPI GGATC 2 cut(s) 658, 926
BspT107I GGYRCC 1 cut(s) 676
BspTI CTTAAG 1 cut(s) 623
BsrBI CCGCTC 2 cut(s) 355, 429
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 2 cut(s) 362, 370
BsrI ACTGG 5 cut(s) 257, 292, 680, 688, 734
BssAI RCCGGY 2 cut(s) 362, 370
BssECI CCNNGG 3 cut(s) 339, 505, 818
BssMI GATC 2 cut(s) 650, 931
BssSI CACGAG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 819, 912
Bst6I CTCTTC 2 cut(s) 140, 428
BstAFI CTTAAG 1 cut(s) 623
BstAPI GCANNNNNTGC 2 cut(s) 218, 956
BstC8I GCNNGC 3 cut(s) 360, 364, 368
BstDEI CTNAG 5 cut(s) 383, 396, 597, 707, 812
BstF5I GGATG 4 cut(s) 176, 853, 867, 895
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 2 cut(s) 653, 934
BstMBI GATC 2 cut(s) 650, 931
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 209, 218, 272, 956
BstNI CCWGG 2 cut(s) 819, 912
BstSCI CCNGG 5 cut(s) 221, 319, 505, 817, 910
BstSLI GKGCMC 1 cut(s) 321
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 2 cut(s) 313, 943
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 650
BstXI CCANNNNNNTGG 2 cut(s) 101, 532
BstYI RGATCY 1 cut(s) 650
BsuRI GGCC 4 cut(s) 319, 344, 362, 581
BtgZI GCGATG 1 cut(s) 327
BtsCI GGATG 4 cut(s) 176, 853, 867, 895
BtsI GCAGTG 2 cut(s) 94, 476
BtsIMutI CAGTG 2 cut(s) 94, 476
Cac8I GCNNGC 3 cut(s) 360, 364, 368
CaiI CAGNNNCTG 1 cut(s) 956
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 2 cut(s) 362, 370
Cfr13I GGNCC 4 cut(s) 317, 318, 502, 522
CseI GACGC 1 cut(s) 476
CviAII CATG 2 cut(s) 218, 331
DdeI CTNAG 5 cut(s) 383, 396, 597, 707, 812
DpnI GATC 2 cut(s) 652, 933
DpnII GATC 2 cut(s) 650, 931
DriI GACNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 140, 428
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 2 cut(s) 140, 428
Eco24I GRGCYC 2 cut(s) 268, 321
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 502, 522
Eco57I CTGAAG 1 cut(s) 568
Eco88I CYCGRG 1 cut(s) 842
EcoRI GAATTC 2 cut(s) 301, 401
EcoRII CCWGG 2 cut(s) 817, 910
EcoRV GATATC 1 cut(s) 922
EcoT38I GRGCYC 2 cut(s) 268, 321
FaeI CATG 2 cut(s) 221, 334
FaiI YATR 5 cut(s) 219, 332, 378, 489, 500
FaqI GGGAC 1 cut(s) 836
FatI CATG 2 cut(s) 217, 330
FauI CCCGC 2 cut(s) 422, 512
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 4 cut(s) 327, 356, 393, 957
FokI GGATG 4 cut(s) 163, 860, 874, 902
FriOI GRGCYC 2 cut(s) 268, 321
Fsp4HI GCNGC 4 cut(s) 327, 356, 393, 957
FspBI CTAG 1 cut(s) 306
GlaI GCGC 1 cut(s) 621
GluI GCNGC 4 cut(s) 327, 356, 393, 957
HaeIII GGCC 4 cut(s) 319, 344, 362, 581
HapII CCGG 5 cut(s) 222, 321, 363, 371, 507
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 622
Hin1II CATG 2 cut(s) 221, 334
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 7 cut(s) 239, 436, 473, 592, 599, 703, 793
HpaII CCGG 5 cut(s) 222, 321, 363, 371, 507
HphI GGTGA 2 cut(s) 30, 70
Hpy166II GTNNAC 2 cut(s) 280, 336
Hpy188I TCNGA 2 cut(s) 384, 658
Hpy188III TCNNGA 8 cut(s) 298, 457, 596, 613, 745, 774, 785, 842
Hpy8I GTNNAC 2 cut(s) 280, 336
HpyAV CCTTC 9 cut(s) 85, 159, 454, 592, 625, 631, 637, 742, 859
HpyCH4IV ACGT 1 cut(s) 894
HpyCH4V TGCA 1 cut(s) 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 218, 272, 956
HpyF3I CTNAG 5 cut(s) 383, 396, 597, 707, 812
HpySE526I ACGT 1 cut(s) 894
Hsp92II CATG 2 cut(s) 221, 334
HspAI GCGC 1 cut(s) 620
KroI GCCGGC 1 cut(s) 362
KroNI GCCGGC 1 cut(s) 364
Kzo9I GATC 2 cut(s) 650, 931
LmnI GCTCC 4 cut(s) 37, 323, 352, 696
Lsp1109I GCAGC 2 cut(s) 313, 943
LweI GCATC 1 cut(s) 943
MaeI CTAG 1 cut(s) 306
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 1 cut(s) 474
MalI GATC 2 cut(s) 652, 933
MbiI CCGCTC 2 cut(s) 355, 429
MboI GATC 2 cut(s) 650, 931
MboII GAAGA 5 cut(s) 127, 144, 445, 594, 645
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 268, 321
MluCI AATT 4 cut(s) 270, 301, 401, 797
MlyI GAGTC 3 cut(s) 445, 482, 608
MmeI TCCRAC 2 cut(s) 91, 210
MroNI GCCGGC 1 cut(s) 362
MseI TTAA 2 cut(s) 624, 800
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 5 cut(s) 222, 321, 363, 371, 507
MspR9I CCNGG 5 cut(s) 223, 321, 507, 819, 912
Mva1269I GAATGC 3 cut(s) 212, 516, 622
MvaI CCWGG 2 cut(s) 819, 912
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 4 cut(s) 209, 218, 272, 956
NaeI GCCGGC 1 cut(s) 364
NciI CCSGG 3 cut(s) 223, 321, 507
NdeII GATC 2 cut(s) 650, 931
NgoMIV GCCGGC 1 cut(s) 362
NlaIII CATG 2 cut(s) 221, 334
NlaIV GGNNCC 4 cut(s) 319, 504, 523, 678
NmuCI GTSAC 1 cut(s) 474
PaeR7I CTCGAG 1 cut(s) 842
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 3 cut(s) 212, 516, 622
PdiI GCCGGC 1 cut(s) 364
PfeI GAWTC 4 cut(s) 239, 592, 703, 793
PfoI TCCNGGA 1 cut(s) 910
PkrI GCNGC 4 cut(s) 328, 357, 394, 958
PleI GAGTC 3 cut(s) 444, 481, 607
PpsI GAGTC 3 cut(s) 444, 481, 607
Psp6I CCWGG 2 cut(s) 817, 910
PspGI CCWGG 2 cut(s) 817, 910
PspN4I GGNNCC 4 cut(s) 319, 504, 523, 678
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 4 cut(s) 317, 318, 502, 522
PstNI CAGNNNCTG 1 cut(s) 956
PsuI RGATCY 1 cut(s) 650
SalI GTCGAC 1 cut(s) 334
SaqAI TTAA 2 cut(s) 624, 800
SatI GCNGC 4 cut(s) 327, 356, 393, 957
Sau3AI GATC 2 cut(s) 650, 931
Sau96I GGNCC 4 cut(s) 317, 318, 502, 522
SchI GAGTC 3 cut(s) 445, 482, 608
ScrFI CCNGG 5 cut(s) 223, 321, 507, 819, 912
SduI GDGCHC 2 cut(s) 268, 321
SfaNI GCATC 1 cut(s) 943
Sfr274I CTCGAG 1 cut(s) 842
SinI GGWCC 2 cut(s) 502, 522
SlaI CTCGAG 1 cut(s) 842
SmlI CTYRAG 5 cut(s) 482, 563, 606, 623, 842
SmoI CTYRAG 5 cut(s) 482, 563, 606, 623, 842
Sse9I AATT 4 cut(s) 270, 301, 401, 797
SsiI CCGC 6 cut(s) 41, 355, 392, 429, 470, 519
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 5 cut(s) 221, 319, 505, 817, 910
TaiI ACGT 1 cut(s) 897
TaqI TCGA 6 cut(s) 268, 335, 348, 425, 692, 843
TaqII GACCGA 2 cut(s) 353, 871
TasI AATT 4 cut(s) 270, 301, 401, 797
TauI GCSGC 2 cut(s) 358, 395
TfiI GAWTC 4 cut(s) 239, 592, 703, 793
Tru1I TTAA 2 cut(s) 624, 800
Tru9I TTAA 2 cut(s) 624, 800
TscAI CASTG 2 cut(s) 101, 483
TseFI GTSAC 1 cut(s) 474
TseI GCWGC 2 cut(s) 326, 956
Tsp45I GTSAC 1 cut(s) 474
TspDTI ATGAA 2 cut(s) 17, 878
TspRI CASTG 2 cut(s) 101, 483
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 502, 522
XapI RAATTY 2 cut(s) 301, 401
XhoI CTCGAG 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 335
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.