Rh4AG174200

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007361
Physical Location & Seq
Forward (+)
43690625 .. 43697079
6455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG174200.1

Sequence Viewer

Length: 717 bp
ATGAACTTGGGCAACCCCAATAACCCAAATGCTCCCTCACCACCACCCCCACCTCAACTCAACCTCCAACAAGCCTTCACCTCCCTCCAAACCCACTGCTCTGGTTTCCTCCACCACCTCTCTCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCACCTCCAATCATCCCTCTCCAACCTCCAAAACCAAGCCAAACACGCATTCCATGCCGGGTTTTCGGGCTTTGATTCCTCCAAGCAGAACCCAGTTTGGGCTCGAATTGCTGTAAACAACAAAACCCAGTTCCCGAATTCCAGTGGCGATGGGCCCGGAGCTGCCATGTCGACCGAGGCCATCGAGGAGCGGCTGGCCGGCGTGCCGGTTTATGCTCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGCGGGAAGAGTCTTGGGTTGTTTTGTTTCAAGAAGGAGGACGCGGAGTCACTGCTTGAGTATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTGGTGGCTGTGGCTTTGAATAAGGTTTTCCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGATTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAGGAGAAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGGTTCTCCTCTTAGCTTTTCTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

238

Amino Acids

25.54

Weight (kDa)

7.83

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 59 - 234 6.4e-55 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 676
AccBSI CCGCTC 2 cut(s) 355, 429
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 470
AciI CCGC 5 cut(s) 355, 392, 429, 470, 519
AclWI GGATC 1 cut(s) 658
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 2 cut(s) 301, 401
AcuI CTGAAG 1 cut(s) 568
AfiI CCNNNNNNNGG 2 cut(s) 262, 565
AflII CTTAAG 1 cut(s) 623
AgsI TTSAA 3 cut(s) 139, 457, 547
AhdI GACNNNNNGTC 1 cut(s) 472
AjnI CCWGG 1 cut(s) 687
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 3 cut(s) 326, 562, 704
AluI AGCT 3 cut(s) 326, 562, 704
AlwI GGATC 1 cut(s) 658
AoxI GGCC 4 cut(s) 317, 342, 360, 579
ApaI GGGCCC 1 cut(s) 321
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 2 cut(s) 301, 401
AspLEI GCGC 1 cut(s) 622
AspS9I GGNCC 4 cut(s) 317, 318, 502, 522
AsuC2I CCSGG 3 cut(s) 223, 321, 507
AsuHPI GGTGA 2 cut(s) 30, 70
AvaII GGWCC 2 cut(s) 502, 522
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 676
BanII GRGCYC 2 cut(s) 268, 321
BbsI GAAGAC 1 cut(s) 594
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 313
BccI CCATC 3 cut(s) 308, 353, 566
BciT130I CCWGG 1 cut(s) 689
BcnI CCSGG 3 cut(s) 223, 321, 507
BfrI CTTAAG 1 cut(s) 623
BisI GCNGC 3 cut(s) 327, 356, 393
BlsI GCNGC 3 cut(s) 328, 357, 394
Bme1390I CCNGG 4 cut(s) 223, 321, 507, 689
Bme18I GGWCC 2 cut(s) 502, 522
BmeRI GACNNNNNGTC 1 cut(s) 472
BmgT120I GGNCC 4 cut(s) 317, 318, 502, 522
BmiI GGNNCC 4 cut(s) 319, 504, 523, 678
BmrFI CCNGG 4 cut(s) 223, 321, 507, 689
BmrI ACTGGG 2 cut(s) 251, 286
BmuI ACTGGG 2 cut(s) 251, 286
BpiI GAAGAC 1 cut(s) 594
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 3 cut(s) 503, 548, 591
BpuMI CCSGG 3 cut(s) 223, 321, 507
BsaJI CCNNGG 2 cut(s) 339, 505
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 565
Bse118I RCCGGY 2 cut(s) 362, 370
Bse1I ACTGG 4 cut(s) 257, 292, 306, 680
Bse3DI GCAATG 1 cut(s) 394
BseBI CCWGG 1 cut(s) 689
BseDI CCNNGG 2 cut(s) 339, 505
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 262, 565
BseMI GCAATG 1 cut(s) 394
BseMII CTCAG 3 cut(s) 374, 387, 588
BseNI ACTGG 4 cut(s) 257, 292, 306, 680
BseRI GAGGAG 2 cut(s) 365, 686
BseSI GKGCMC 1 cut(s) 321
BseXI GCAGC 1 cut(s) 313
Bsh1236I CGCG 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 4 cut(s) 319, 344, 362, 581
BshNI GGYRCC 1 cut(s) 676
BsiEI CGRYCG 1 cut(s) 339
BsiSI CCGG 5 cut(s) 222, 321, 363, 371, 507
BslI CCNNNNNNNGG 2 cut(s) 262, 565
BsmI GAATGC 3 cut(s) 212, 516, 622
BsnI GGCC 4 cut(s) 319, 344, 362, 581
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 268, 321
Bsp143I GATC 1 cut(s) 650
BspACI CCGC 5 cut(s) 355, 392, 429, 470, 519
BspANI GGCC 4 cut(s) 319, 344, 362, 581
BspCNI CTCAG 3 cut(s) 375, 388, 589
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 4 cut(s) 319, 504, 523, 678
BspPI GGATC 1 cut(s) 658
BspT107I GGYRCC 1 cut(s) 676
BspTI CTTAAG 1 cut(s) 623
BsrBI CCGCTC 2 cut(s) 355, 429
BsrDI GCAATG 1 cut(s) 394
BsrFI RCCGGY 2 cut(s) 362, 370
BsrI ACTGG 4 cut(s) 257, 292, 306, 680
BssAI RCCGGY 2 cut(s) 362, 370
BssECI CCNNGG 2 cut(s) 339, 505
BssMI GATC 1 cut(s) 650
Bst2UI CCWGG 1 cut(s) 689
Bst6I CTCTTC 2 cut(s) 140, 428
BstAFI CTTAAG 1 cut(s) 623
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 3 cut(s) 360, 364, 368
BstDEI CTNAG 4 cut(s) 383, 396, 597, 700
BstF5I GGATG 1 cut(s) 176
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 622
BstKTI GATC 1 cut(s) 653
BstMBI GATC 1 cut(s) 650
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 3 cut(s) 209, 218, 272
BstNI CCWGG 1 cut(s) 689
BstSCI CCNGG 4 cut(s) 221, 319, 505, 687
BstSLI GKGCMC 1 cut(s) 321
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 1 cut(s) 313
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 650
BstXI CCANNNNNNTGG 2 cut(s) 101, 532
BstYI RGATCY 1 cut(s) 650
BsuRI GGCC 4 cut(s) 319, 344, 362, 581
BtgZI GCGATG 1 cut(s) 327
BtsCI GGATG 1 cut(s) 176
BtsI GCAGTG 2 cut(s) 94, 476
BtsIMutI CAGTG 3 cut(s) 94, 313, 476
Cac8I GCNNGC 3 cut(s) 360, 364, 368
CfoI GCGC 1 cut(s) 622
Cfr10I RCCGGY 2 cut(s) 362, 370
Cfr13I GGNCC 4 cut(s) 317, 318, 502, 522
CseI GACGC 1 cut(s) 476
CviAII CATG 2 cut(s) 218, 331
DdeI CTNAG 4 cut(s) 383, 396, 597, 700
DpnI GATC 1 cut(s) 652
DpnII GATC 1 cut(s) 650
DriI GACNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 140, 428
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 2 cut(s) 140, 428
Eco24I GRGCYC 2 cut(s) 268, 321
Eco47I GGWCC 2 cut(s) 502, 522
Eco57I CTGAAG 1 cut(s) 568
EcoRI GAATTC 2 cut(s) 301, 401
EcoRII CCWGG 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 268, 321
FaeI CATG 2 cut(s) 221, 334
FaiI YATR 5 cut(s) 219, 332, 378, 489, 500
FatI CATG 2 cut(s) 217, 330
FauI CCCGC 2 cut(s) 422, 512
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 3 cut(s) 327, 356, 393
FokI GGATG 1 cut(s) 163
FriOI GRGCYC 2 cut(s) 268, 321
Fsp4HI GCNGC 3 cut(s) 327, 356, 393
GlaI GCGC 1 cut(s) 621
GluI GCNGC 3 cut(s) 327, 356, 393
HaeIII GGCC 4 cut(s) 319, 344, 362, 581
HapII CCGG 5 cut(s) 222, 321, 363, 371, 507
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 622
Hin1II CATG 2 cut(s) 221, 334
Hin6I GCGC 1 cut(s) 620
HinP1I GCGC 1 cut(s) 620
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 5 cut(s) 239, 436, 473, 592, 599
HpaII CCGG 5 cut(s) 222, 321, 363, 371, 507
HphI GGTGA 2 cut(s) 30, 70
Hpy166II GTNNAC 2 cut(s) 280, 336
Hpy188I TCNGA 2 cut(s) 384, 658
Hpy188III TCNNGA 4 cut(s) 298, 457, 596, 613
Hpy8I GTNNAC 2 cut(s) 280, 336
HpyAV CCTTC 7 cut(s) 85, 159, 454, 592, 625, 631, 637
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 218, 272
HpyF3I CTNAG 4 cut(s) 383, 396, 597, 700
Hsp92II CATG 2 cut(s) 221, 334
HspAI GCGC 1 cut(s) 620
KroI GCCGGC 1 cut(s) 362
KroNI GCCGGC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 650
LmnI GCTCC 3 cut(s) 37, 323, 352
Lsp1109I GCAGC 1 cut(s) 313
MaeIII GTNAC 1 cut(s) 474
MalI GATC 1 cut(s) 652
MbiI CCGCTC 2 cut(s) 355, 429
MboI GATC 1 cut(s) 650
MboII GAAGA 5 cut(s) 127, 144, 445, 594, 645
MfeI CAATTG 1 cut(s) 712
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 2 cut(s) 268, 321
MluCI AATT 4 cut(s) 270, 301, 401, 712
MlyI GAGTC 3 cut(s) 445, 482, 608
MmeI TCCRAC 2 cut(s) 91, 210
MroNI GCCGGC 1 cut(s) 362
MseI TTAA 1 cut(s) 624
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 5 cut(s) 222, 321, 363, 371, 507
MspR9I CCNGG 4 cut(s) 223, 321, 507, 689
MunI CAATTG 1 cut(s) 712
Mva1269I GAATGC 3 cut(s) 212, 516, 622
MvaI CCWGG 1 cut(s) 689
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 3 cut(s) 209, 218, 272
NaeI GCCGGC 1 cut(s) 364
NciI CCSGG 3 cut(s) 223, 321, 507
NdeII GATC 1 cut(s) 650
NgoMIV GCCGGC 1 cut(s) 362
NlaIII CATG 2 cut(s) 221, 334
NlaIV GGNNCC 4 cut(s) 319, 504, 523, 678
NmuCI GTSAC 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 3 cut(s) 212, 516, 622
PdiI GCCGGC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 239, 592
PkrI GCNGC 3 cut(s) 328, 357, 394
PleI GAGTC 3 cut(s) 444, 481, 607
PpsI GAGTC 3 cut(s) 444, 481, 607
Psp6I CCWGG 1 cut(s) 687
PspGI CCWGG 1 cut(s) 687
PspN4I GGNNCC 4 cut(s) 319, 504, 523, 678
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 4 cut(s) 317, 318, 502, 522
PsuI RGATCY 1 cut(s) 650
SalI GTCGAC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 3 cut(s) 327, 356, 393
Sau3AI GATC 1 cut(s) 650
Sau96I GGNCC 4 cut(s) 317, 318, 502, 522
SchI GAGTC 3 cut(s) 445, 482, 608
ScrFI CCNGG 4 cut(s) 223, 321, 507, 689
SduI GDGCHC 2 cut(s) 268, 321
SinI GGWCC 2 cut(s) 502, 522
SmlI CTYRAG 4 cut(s) 482, 563, 606, 623
SmoI CTYRAG 4 cut(s) 482, 563, 606, 623
Sse9I AATT 4 cut(s) 270, 301, 401, 712
SsiI CCGC 5 cut(s) 355, 392, 429, 470, 519
StyD4I CCNGG 4 cut(s) 221, 319, 505, 687
TaqI TCGA 4 cut(s) 268, 335, 348, 425
TaqII GACCGA 1 cut(s) 353
TasI AATT 4 cut(s) 270, 301, 401, 712
TauI GCSGC 2 cut(s) 358, 395
TfiI GAWTC 2 cut(s) 239, 592
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 3 cut(s) 101, 313, 483
TseFI GTSAC 1 cut(s) 474
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 474
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 101, 313, 483
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 502, 522
XapI RAATTY 2 cut(s) 301, 401
XmiI GTMKAC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.