Rroxscaffold_5G00356770

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007361
Physical Location & Seq
Forward (+)
36152319 .. 36158598
6280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356770.1

Sequence Viewer

Length: 1044 bp
ATGAACTTGGGCAACCCCAATAACCCAAATGCTCCCTCACCACCACCACCTCAACTCAACCTCCAACAAGCCTTCACCTCCCTCCAAAACCACTGCTCTGGTTTCCTCCACCACCTCTCTCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCACCTCCAATCATCCCTCTCCAACCTCCAAAACCAAGCCAAACACGCATTCCATGCCGGGTTTTGGGGCTTTGATTCCTCCAAGCAGAACCCAGTTTGGGCTCGAATTGCTGTAAACAACAAAACCCAGTTCCCGAATTCTAGCGGCGATGGGCCCGGAGCTGCCATGTCGATCGAGGCCATCGAGGAGCGGCTGGCCGGCGTGCCGGTTTATGCTCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGCGGGAAGAGTCTTGGGTTGTTTTGTTTCAAGAAGGAGGACGCGGAGTCACTGCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGGAGCGGGTCCAAAGTTGTGGCTGTGGCTTTGAATAAGGTTTTCCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGATTCCTGAGTCTTCTCAAGTCAAGTATGCGCTTAAGGAAAAGGAGAAGGAACAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGTCGAGGAGCTTGATTCTAAGAAGCCAAAGCAAGAGCTATCGTCCTGTATTTTTCAGGAAGGAGGATTTAGAAAACTCACTATCAAGAGCTTCTCGTGAGCAGAATCAATTAAATCCTTTACTTAGACCAGGGGACATTCAGGTTGCTGTTCTCGAGGATGTAATAAAGGGGATGAAGGAGGGTTCTACATCGGTCTGGGATGACGTTGTTTTTATCCCCCTGGATTTGATATCTCTACTGATCCCACCAAGCAATAGGCAGATGCTACAAAATAGCAGGCATTGTCCTCATTGGCATGAACATCATTCCAATACAAACTGCAACCTGTTAGTTTATATTATTGGCATTAGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

347

Amino Acids

38.32

Weight (kDa)

7.78

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 58 - 302 3.8e-83 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 673
AccBSI CCGCTC 3 cut(s) 352, 426, 516
AccII CGCG 1 cut(s) 467
AciI CCGC 7 cut(s) 306, 352, 389, 426, 467, 511, 516
AclWI GGATC 2 cut(s) 655, 922
AcoI YGGCCR 1 cut(s) 357
AcsI RAATTY 2 cut(s) 298, 398
AcuI CTGAAG 1 cut(s) 565
AfiI CCNNNNNNNGG 4 cut(s) 225, 259, 510, 562
AflII CTTAAG 1 cut(s) 620
AgsI TTSAA 3 cut(s) 136, 454, 544
AhdI GACNNNNNGTC 1 cut(s) 469
AjnI CCWGG 2 cut(s) 814, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 6 cut(s) 323, 559, 696, 723, 776, 1038
AluI AGCT 6 cut(s) 323, 559, 696, 723, 776, 1038
AlwI GGATC 2 cut(s) 655, 922
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 4 cut(s) 314, 339, 357, 576
ApaI GGGCCC 1 cut(s) 318
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 2 cut(s) 298, 398
AspLEI GCGC 1 cut(s) 619
AspS9I GGNCC 4 cut(s) 314, 315, 499, 519
AsuC2I CCSGG 3 cut(s) 220, 318, 504
AsuHPI GGTGA 2 cut(s) 30, 67
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 2 cut(s) 499, 519
BaeGI GKGCMC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 673
BanII GRGCYC 2 cut(s) 265, 318
BauI CACGAG 1 cut(s) 780
BbsI GAAGAC 1 cut(s) 591
BbvCI CCTCAGC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 310
BccI CCATC 3 cut(s) 305, 350, 563
BciT130I CCWGG 2 cut(s) 816, 908
BcnI CCSGG 3 cut(s) 220, 318, 504
BfaI CTAG 1 cut(s) 303
BfrI CTTAAG 1 cut(s) 620
BisI GCNGC 4 cut(s) 307, 324, 353, 390
BlsI GCNGC 4 cut(s) 308, 325, 354, 391
Bme1390I CCNGG 5 cut(s) 220, 318, 504, 816, 908
Bme18I GGWCC 2 cut(s) 499, 519
BmeRI GACNNNNNGTC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 4 cut(s) 314, 315, 499, 519
BmiI GGNNCC 4 cut(s) 316, 501, 520, 675
BmrFI CCNGG 5 cut(s) 220, 318, 504, 816, 908
BmrI ACTGGG 2 cut(s) 248, 283
BmsI GCATC 1 cut(s) 939
BmuI ACTGGG 2 cut(s) 248, 283
BpiI GAAGAC 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 393
BpuEI CTTGAG 3 cut(s) 500, 545, 588
BpuMI CCSGG 3 cut(s) 220, 318, 504
BsaJI CCNNGG 3 cut(s) 502, 815, 906
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 259, 510, 562
Bse118I RCCGGY 2 cut(s) 359, 367
Bse1I ACTGG 4 cut(s) 254, 289, 677, 685
Bse3DI GCAATG 1 cut(s) 391
BseBI CCWGG 2 cut(s) 816, 908
BseDI CCNNGG 3 cut(s) 502, 815, 906
BseGI GGATG 4 cut(s) 173, 850, 864, 892
BseLI CCNNNNNNNGG 4 cut(s) 225, 259, 510, 562
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 3 cut(s) 371, 384, 585
BseNI ACTGG 4 cut(s) 254, 289, 677, 685
BseRI GAGGAG 2 cut(s) 362, 706
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 1 cut(s) 310
Bsh1236I CGCG 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 4 cut(s) 316, 341, 359, 578
BshNI GGYRCC 1 cut(s) 673
BsiEI CGRYCG 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 5 cut(s) 219, 318, 360, 368, 504
BslFI GGGAC 1 cut(s) 833
BslI CCNNNNNNNGG 4 cut(s) 225, 259, 510, 562
BsmFI GGGAC 1 cut(s) 833
BsmI GAATGC 2 cut(s) 209, 513
BsnI GGCC 4 cut(s) 316, 341, 359, 578
BsoBI CYCGRG 1 cut(s) 839
Bsp120I GGGCCC 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 265, 318
Bsp143I GATC 3 cut(s) 333, 647, 927
BspACI CCGC 7 cut(s) 306, 352, 389, 426, 467, 511, 516
BspANI GGCC 4 cut(s) 316, 341, 359, 578
BspCNI CTCAG 3 cut(s) 372, 385, 586
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 4 cut(s) 316, 501, 520, 675
BspPI GGATC 2 cut(s) 655, 922
BspT107I GGYRCC 1 cut(s) 673
BspTI CTTAAG 1 cut(s) 620
BsrBI CCGCTC 3 cut(s) 352, 426, 516
BsrDI GCAATG 1 cut(s) 391
BsrFI RCCGGY 2 cut(s) 359, 367
BsrI ACTGG 4 cut(s) 254, 289, 677, 685
BssAI RCCGGY 2 cut(s) 359, 367
BssECI CCNNGG 3 cut(s) 502, 815, 906
BssMI GATC 3 cut(s) 333, 647, 927
BssSI CACGAG 1 cut(s) 780
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 2 cut(s) 816, 908
Bst6I CTCTTC 2 cut(s) 137, 425
BstAFI CTTAAG 1 cut(s) 620
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 4 cut(s) 357, 361, 365, 965
BstDEI CTNAG 5 cut(s) 380, 393, 594, 704, 809
BstF5I GGATG 4 cut(s) 173, 850, 864, 892
BstFNI CGCG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 619
BstKTI GATC 3 cut(s) 336, 650, 930
BstMBI GATC 3 cut(s) 333, 647, 927
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 3 cut(s) 206, 215, 269
BstNI CCWGG 2 cut(s) 816, 908
BstSCI CCNGG 5 cut(s) 218, 316, 502, 814, 906
BstSLI GKGCMC 1 cut(s) 318
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 1 cut(s) 310
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 2 cut(s) 98, 529
BstYI RGATCY 1 cut(s) 647
BsuRI GGCC 4 cut(s) 316, 341, 359, 578
BtgZI GCGATG 1 cut(s) 324
BtsCI GGATG 4 cut(s) 173, 850, 864, 892
BtsI GCAGTG 2 cut(s) 91, 473
BtsIMutI CAGTG 2 cut(s) 91, 473
Cac8I GCNNGC 4 cut(s) 357, 361, 365, 965
CfoI GCGC 1 cut(s) 619
Cfr10I RCCGGY 2 cut(s) 359, 367
Cfr13I GGNCC 4 cut(s) 314, 315, 499, 519
CseI GACGC 1 cut(s) 473
CviAII CATG 3 cut(s) 215, 328, 983
DdeI CTNAG 5 cut(s) 380, 393, 594, 704, 809
DpnI GATC 3 cut(s) 335, 649, 929
DpnII GATC 3 cut(s) 333, 647, 927
DriI GACNNNNNGTC 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 137, 425
Eam1105I GACNNNNNGTC 1 cut(s) 469
EarI CTCTTC 2 cut(s) 137, 425
Eco24I GRGCYC 2 cut(s) 265, 318
Eco32I GATATC 1 cut(s) 918
Eco47I GGWCC 2 cut(s) 499, 519
Eco57I CTGAAG 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 839
EcoRI GAATTC 2 cut(s) 298, 398
EcoRII CCWGG 2 cut(s) 814, 906
EcoRV GATATC 1 cut(s) 918
EcoT38I GRGCYC 2 cut(s) 265, 318
FaeI CATG 3 cut(s) 218, 331, 986
FaiI YATR 8 cut(s) 216, 329, 375, 486, 497, 615, 984, 1023
FaqI GGGAC 1 cut(s) 833
FatI CATG 3 cut(s) 214, 327, 982
FauI CCCGC 2 cut(s) 419, 509
Fnu4HI GCNGC 4 cut(s) 307, 324, 353, 390
FokI GGATG 4 cut(s) 160, 857, 871, 899
FriOI GRGCYC 2 cut(s) 265, 318
Fsp4HI GCNGC 4 cut(s) 307, 324, 353, 390
FspBI CTAG 1 cut(s) 303
GlaI GCGC 1 cut(s) 618
GluI GCNGC 4 cut(s) 307, 324, 353, 390
HaeIII GGCC 4 cut(s) 316, 341, 359, 578
HapII CCGG 5 cut(s) 219, 318, 360, 368, 504
HgaI GACGC 1 cut(s) 473
HhaI GCGC 1 cut(s) 619
Hin1II CATG 3 cut(s) 218, 331, 986
Hin6I GCGC 1 cut(s) 617
HinP1I GCGC 1 cut(s) 617
HinfI GANTC 7 cut(s) 236, 433, 470, 589, 596, 700, 790
HpaII CCGG 5 cut(s) 219, 318, 360, 368, 504
HphI GGTGA 2 cut(s) 30, 67
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 2 cut(s) 381, 655
Hpy188III TCNNGA 7 cut(s) 295, 454, 593, 742, 771, 782, 839
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 7 cut(s) 82, 156, 451, 589, 628, 739, 856
HpyCH4IV ACGT 1 cut(s) 891
HpyCH4V TGCA 1 cut(s) 1008
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 215, 269
HpyF3I CTNAG 5 cut(s) 380, 393, 594, 704, 809
HpySE526I ACGT 1 cut(s) 891
Hsp92II CATG 3 cut(s) 218, 331, 986
HspAI GCGC 1 cut(s) 617
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 333, 647, 927
LmnI GCTCC 5 cut(s) 37, 320, 349, 513, 693
Lsp1109I GCAGC 1 cut(s) 310
LweI GCATC 1 cut(s) 939
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 891
MaeIII GTNAC 1 cut(s) 471
MalI GATC 3 cut(s) 335, 649, 929
MbiI CCGCTC 3 cut(s) 352, 426, 516
MboI GATC 3 cut(s) 333, 647, 927
MboII GAAGA 5 cut(s) 124, 141, 442, 591, 642
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 2 cut(s) 265, 318
MluCI AATT 4 cut(s) 267, 298, 398, 794
MlyI GAGTC 3 cut(s) 442, 479, 605
MmeI TCCRAC 2 cut(s) 88, 207
MroNI GCCGGC 1 cut(s) 359
MseI TTAA 2 cut(s) 621, 797
MslI CAYNNNNRTG 1 cut(s) 981
MspCI CTTAAG 1 cut(s) 620
MspI CCGG 5 cut(s) 219, 318, 360, 368, 504
MspR9I CCNGG 5 cut(s) 220, 318, 504, 816, 908
Mva1269I GAATGC 2 cut(s) 209, 513
MvaI CCWGG 2 cut(s) 816, 908
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 3 cut(s) 206, 215, 269
NaeI GCCGGC 1 cut(s) 361
NciI CCSGG 3 cut(s) 220, 318, 504
NdeII GATC 3 cut(s) 333, 647, 927
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 3 cut(s) 218, 331, 986
NlaIV GGNNCC 4 cut(s) 316, 501, 520, 675
NmuCI GTSAC 1 cut(s) 471
PaeR7I CTCGAG 1 cut(s) 839
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 419
PctI GAATGC 2 cut(s) 209, 513
PdiI GCCGGC 1 cut(s) 361
PfeI GAWTC 4 cut(s) 236, 589, 700, 790
PkrI GCNGC 4 cut(s) 308, 325, 354, 391
Ple19I CGATCG 1 cut(s) 336
PleI GAGTC 3 cut(s) 441, 478, 604
PpsI GAGTC 3 cut(s) 441, 478, 604
Psp6I CCWGG 2 cut(s) 814, 906
PspGI CCWGG 2 cut(s) 814, 906
PspN4I GGNNCC 4 cut(s) 316, 501, 520, 675
PspOMI GGGCCC 1 cut(s) 314
PspPI GGNCC 4 cut(s) 314, 315, 499, 519
PsuI RGATCY 1 cut(s) 647
PvuI CGATCG 1 cut(s) 336
RseI CAYNNNNRTG 1 cut(s) 981
SaqAI TTAA 2 cut(s) 621, 797
SatI GCNGC 4 cut(s) 307, 324, 353, 390
Sau3AI GATC 3 cut(s) 333, 647, 927
Sau96I GGNCC 4 cut(s) 314, 315, 499, 519
SchI GAGTC 3 cut(s) 442, 479, 605
ScrFI CCNGG 5 cut(s) 220, 318, 504, 816, 908
SduI GDGCHC 2 cut(s) 265, 318
SfaNI GCATC 1 cut(s) 939
Sfr274I CTCGAG 1 cut(s) 839
SinI GGWCC 2 cut(s) 499, 519
SlaI CTCGAG 1 cut(s) 839
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 5 cut(s) 479, 560, 603, 620, 839
SmoI CTYRAG 5 cut(s) 479, 560, 603, 620, 839
Sse9I AATT 4 cut(s) 267, 298, 398, 794
SsiI CCGC 7 cut(s) 306, 352, 389, 426, 467, 511, 516
SspMI CTAG 1 cut(s) 303
StyD4I CCNGG 5 cut(s) 218, 316, 502, 814, 906
TaiI ACGT 1 cut(s) 894
TaqI TCGA 7 cut(s) 265, 332, 336, 345, 422, 689, 840
TaqII GACCGA 1 cut(s) 868
TasI AATT 4 cut(s) 267, 298, 398, 794
TauI GCSGC 3 cut(s) 309, 355, 392
TfiI GAWTC 4 cut(s) 236, 589, 700, 790
Tru1I TTAA 2 cut(s) 621, 797
Tru9I TTAA 2 cut(s) 621, 797
TscAI CASTG 2 cut(s) 98, 480
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 3 cut(s) 17, 875, 999
TspRI CASTG 2 cut(s) 98, 480
Vha464I CTTAAG 1 cut(s) 620
VpaK11BI GGWCC 2 cut(s) 499, 519
XapI RAATTY 2 cut(s) 298, 398
XhoI CTCGAG 1 cut(s) 839
XspI CTAG 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.