Rw4G014590

TIC 22-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007361
Physical Location & Seq
Forward (+)
33767011 .. 33772489
5479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014590.1

Sequence Viewer

Length: 927 bp
ATGACACCACCACCACCACCTCAACTCAACCTTCAACAAGCCTTCACCTCCCTCCAAACCCACTGCTCTGGTTTCCTCCACCACCTCTCTCATCTCCCTCTCTTCAACCCCAACCCTTCTTCTCTCCAAACCCACCTCCAATCATCCCTCTCCAACCTCCAAAACCAAGCCAAACACGCATTCCATGCCGGGTTTTCGGGCTTTGATTCCTCCAAGCAGAACCCAGTTTGGGCTCGAATTGCTGTAAACAACAAAACCCAGTTCCCGAATTCCAGTGGCGATGGGCCCGGAGCTGCCATGTCGACCGAGGCCATCGAGGAGCGGCTGGCCGGCGTGCCGGTTTATGCTCTGAGCAATGCGGCTGAGGAATTCGTGTTGGTTTCGGGTGCTTCGAGTGGGAAGAGTCTTGGATTGTTTTGTTTCAAGAAGGAAGACGCAGAGTCACTGCTTGAGCATATTAGAGTTATGGACCCCGGAATGCGAAGCGGGTCCAAAGTGGTGGCTGTGGCTTTGAATAAGGTTTTTCAGCTCAAGGTTGATGGGGTGGCCTTCAGGTTGATTCCTGAGTCTTCTCAAGTCAAGAATGCGCTTAAGGAGAAGGAGAAGGGTGGATCTTCTGATAATGGCTTTTTTGGGGTGCCAGTTTTCCAGTCGAGGAGCTTGATTCTAAGAAGCCAAAGCAAGAGCTATCGTCCAGTATTTTTCAGGAAGGAGGATTTAGAAAACTCACTATCAAGAGCTTCTCGTGAGCAGAATCAATTAAATCCTTTACTTAGACCAGGGGACATTCAGGTCGCTGTTCTCGAGGATGTAATAAAGGGGATGAAGGAGGGTTCTACATCGGTCTGGGATGACGTTGTTTTTATCCCTCCTGGATTTGATATCTCTACTGATCCCACCAAGCAACAGGCAGATGCTGCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

308

Amino Acids

33.49

Weight (kDa)

7.81

Isoelectric Point (pI)

53.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 48 - 298 9e-90 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 791
AccB1I GGYRCC 1 cut(s) 637
AccBSI CCGCTC 1 cut(s) 322
AccI GTMKAC 1 cut(s) 302
AciI CCGC 3 cut(s) 322, 359, 486
AclWI GGATC 2 cut(s) 619, 887
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 2 cut(s) 268, 368
AcuI CTGAAG 1 cut(s) 535
AfiI CCNNNNNNNGG 1 cut(s) 229
AflII CTTAAG 1 cut(s) 590
AgsI TTSAA 4 cut(s) 35, 106, 424, 514
AhdI GACNNNNNGTC 1 cut(s) 439
AjnI CCWGG 2 cut(s) 778, 871
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 5 cut(s) 293, 529, 660, 687, 740
AluI AGCT 5 cut(s) 293, 529, 660, 687, 740
AlwI GGATC 2 cut(s) 619, 887
AlwNI CAGNNNCTG 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 803
AoxI GGCC 4 cut(s) 284, 309, 327, 546
ApaI GGGCCC 1 cut(s) 288
ApeKI GCWGC 2 cut(s) 293, 917
ApoI RAATTY 2 cut(s) 268, 368
AspLEI GCGC 1 cut(s) 589
AspS9I GGNCC 4 cut(s) 284, 285, 469, 489
AsuC2I CCSGG 3 cut(s) 190, 288, 474
AsuHPI GGTGA 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 803
AvaII GGWCC 2 cut(s) 469, 489
BaeGI GKGCMC 1 cut(s) 288
BanI GGYRCC 1 cut(s) 637
BanII GRGCYC 2 cut(s) 235, 288
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 2 cut(s) 438, 561
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 280, 904
BccI CCATC 3 cut(s) 275, 320, 533
BciT130I CCWGG 2 cut(s) 780, 873
BcnI CCSGG 3 cut(s) 190, 288, 474
BfrI CTTAAG 1 cut(s) 590
BisI GCNGC 4 cut(s) 294, 323, 360, 918
BlsI GCNGC 4 cut(s) 295, 324, 361, 919
Bme1390I CCNGG 5 cut(s) 190, 288, 474, 780, 873
Bme18I GGWCC 2 cut(s) 469, 489
BmeRI GACNNNNNGTC 1 cut(s) 439
BmeT110I CYCGRG 1 cut(s) 803
BmgT120I GGNCC 4 cut(s) 284, 285, 469, 489
BmiI GGNNCC 4 cut(s) 286, 471, 490, 639
BmrFI CCNGG 5 cut(s) 190, 288, 474, 780, 873
BmrI ACTGGG 2 cut(s) 218, 253
BmsI GCATC 1 cut(s) 904
BmuI ACTGGG 2 cut(s) 218, 253
BpiI GAAGAC 2 cut(s) 438, 561
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 3 cut(s) 470, 515, 558
BpuMI CCSGG 3 cut(s) 190, 288, 474
BsaJI CCNNGG 3 cut(s) 306, 472, 779
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse118I RCCGGY 2 cut(s) 329, 337
Bse1I ACTGG 6 cut(s) 224, 259, 273, 641, 649, 695
Bse3DI GCAATG 1 cut(s) 361
BseBI CCWGG 2 cut(s) 780, 873
BseDI CCNNGG 3 cut(s) 306, 472, 779
BseGI GGATG 4 cut(s) 143, 814, 828, 856
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 3 cut(s) 341, 354, 555
BseNI ACTGG 6 cut(s) 224, 259, 273, 641, 649, 695
BseRI GAGGAG 2 cut(s) 332, 670
BseSI GKGCMC 1 cut(s) 288
BseXI GCAGC 2 cut(s) 280, 904
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 4 cut(s) 286, 311, 329, 548
BshNI GGYRCC 1 cut(s) 637
BsiEI CGRYCG 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 803
BsiSI CCGG 5 cut(s) 189, 288, 330, 338, 474
BslFI GGGAC 1 cut(s) 797
BslI CCNNNNNNNGG 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 797
BsmI GAATGC 3 cut(s) 179, 483, 589
BsnI GGCC 4 cut(s) 286, 311, 329, 548
BsoBI CYCGRG 1 cut(s) 803
Bsp120I GGGCCC 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 235, 288
Bsp143I GATC 2 cut(s) 611, 892
BspACI CCGC 3 cut(s) 322, 359, 486
BspANI GGCC 4 cut(s) 286, 311, 329, 548
BspCNI CTCAG 3 cut(s) 342, 355, 556
BspLI GGNNCC 4 cut(s) 286, 471, 490, 639
BspPI GGATC 2 cut(s) 619, 887
BspT107I GGYRCC 1 cut(s) 637
BspTI CTTAAG 1 cut(s) 590
BsrBI CCGCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 361
BsrFI RCCGGY 2 cut(s) 329, 337
BsrI ACTGG 6 cut(s) 224, 259, 273, 641, 649, 695
BssAI RCCGGY 2 cut(s) 329, 337
BssECI CCNNGG 3 cut(s) 306, 472, 779
BssMI GATC 2 cut(s) 611, 892
BssSI CACGAG 1 cut(s) 744
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 2 cut(s) 780, 873
Bst6I CTCTTC 2 cut(s) 107, 395
BstAFI CTTAAG 1 cut(s) 590
BstAPI GCANNNNNTGC 2 cut(s) 185, 917
BstC8I GCNNGC 3 cut(s) 327, 331, 335
BstDEI CTNAG 5 cut(s) 350, 363, 564, 668, 773
BstF5I GGATG 4 cut(s) 143, 814, 828, 856
BstHHI GCGC 1 cut(s) 589
BstKTI GATC 2 cut(s) 614, 895
BstMBI GATC 2 cut(s) 611, 892
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 4 cut(s) 176, 185, 239, 917
BstNI CCWGG 2 cut(s) 780, 873
BstSCI CCNGG 5 cut(s) 188, 286, 472, 778, 871
BstSLI GKGCMC 1 cut(s) 288
BstV1I GCAGC 2 cut(s) 280, 904
BstV2I GAAGAC 2 cut(s) 438, 561
BstX2I RGATCY 1 cut(s) 611
BstXI CCANNNNNNTGG 2 cut(s) 68, 499
BstYI RGATCY 1 cut(s) 611
BsuRI GGCC 4 cut(s) 286, 311, 329, 548
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 4 cut(s) 143, 814, 828, 856
BtsI GCAGTG 2 cut(s) 61, 443
BtsIMutI CAGTG 3 cut(s) 61, 280, 443
Cac8I GCNNGC 3 cut(s) 327, 331, 335
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 1 cut(s) 589
Cfr10I RCCGGY 2 cut(s) 329, 337
Cfr13I GGNCC 4 cut(s) 284, 285, 469, 489
CseI GACGC 1 cut(s) 443
CviAII CATG 2 cut(s) 185, 298
DdeI CTNAG 5 cut(s) 350, 363, 564, 668, 773
DpnI GATC 2 cut(s) 613, 894
DpnII GATC 2 cut(s) 611, 892
DrdI GACNNNNNNGTC 1 cut(s) 791
DriI GACNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 791
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 107, 395
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 2 cut(s) 107, 395
Eco24I GRGCYC 2 cut(s) 235, 288
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 2 cut(s) 469, 489
Eco57I CTGAAG 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 803
EcoRI GAATTC 2 cut(s) 268, 368
EcoRII CCWGG 2 cut(s) 778, 871
EcoRV GATATC 1 cut(s) 883
EcoT38I GRGCYC 2 cut(s) 235, 288
FaeI CATG 2 cut(s) 188, 301
FaiI YATR 5 cut(s) 186, 299, 345, 456, 467
FaqI GGGAC 1 cut(s) 797
FatI CATG 2 cut(s) 184, 297
FauI CCCGC 1 cut(s) 479
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 4 cut(s) 294, 323, 360, 918
FokI GGATG 4 cut(s) 130, 821, 835, 863
FriOI GRGCYC 2 cut(s) 235, 288
Fsp4HI GCNGC 4 cut(s) 294, 323, 360, 918
GlaI GCGC 1 cut(s) 588
GluI GCNGC 4 cut(s) 294, 323, 360, 918
HaeIII GGCC 4 cut(s) 286, 311, 329, 548
HapII CCGG 5 cut(s) 189, 288, 330, 338, 474
HgaI GACGC 1 cut(s) 443
HhaI GCGC 1 cut(s) 589
Hin1II CATG 2 cut(s) 188, 301
Hin6I GCGC 1 cut(s) 587
HinP1I GCGC 1 cut(s) 587
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HinfI GANTC 7 cut(s) 206, 403, 440, 559, 566, 664, 754
HpaII CCGG 5 cut(s) 189, 288, 330, 338, 474
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 2 cut(s) 247, 303
Hpy188I TCNGA 2 cut(s) 351, 619
Hpy188III TCNNGA 8 cut(s) 265, 424, 563, 580, 706, 735, 746, 803
Hpy8I GTNNAC 2 cut(s) 247, 303
HpyAV CCTTC 9 cut(s) 41, 52, 126, 421, 559, 592, 598, 703, 820
HpyCH4IV ACGT 1 cut(s) 855
HpyCH4V TGCA 1 cut(s) 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 185, 239, 917
HpyF3I CTNAG 5 cut(s) 350, 363, 564, 668, 773
HpySE526I ACGT 1 cut(s) 855
Hsp92II CATG 2 cut(s) 188, 301
HspAI GCGC 1 cut(s) 587
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 2 cut(s) 611, 892
LmnI GCTCC 3 cut(s) 290, 319, 657
Lsp1109I GCAGC 2 cut(s) 280, 904
LweI GCATC 1 cut(s) 904
MaeII ACGT 1 cut(s) 855
MaeIII GTNAC 1 cut(s) 441
MalI GATC 2 cut(s) 613, 894
MbiI CCGCTC 1 cut(s) 322
MboI GATC 2 cut(s) 611, 892
MboII GAAGA 6 cut(s) 94, 111, 412, 443, 561, 606
MflI RGATCY 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 235, 288
MluCI AATT 4 cut(s) 237, 268, 368, 758
MlyI GAGTC 3 cut(s) 412, 449, 575
MmeI TCCRAC 1 cut(s) 177
MroNI GCCGGC 1 cut(s) 329
MseI TTAA 2 cut(s) 591, 761
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 5 cut(s) 189, 288, 330, 338, 474
MspR9I CCNGG 5 cut(s) 190, 288, 474, 780, 873
Mva1269I GAATGC 3 cut(s) 179, 483, 589
MvaI CCWGG 2 cut(s) 780, 873
MwoI GCNNNNNNNGC 4 cut(s) 176, 185, 239, 917
NaeI GCCGGC 1 cut(s) 331
NciI CCSGG 3 cut(s) 190, 288, 474
NdeII GATC 2 cut(s) 611, 892
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 2 cut(s) 188, 301
NlaIV GGNNCC 4 cut(s) 286, 471, 490, 639
NmuCI GTSAC 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 803
PcsI WCGNNNNNNNCGW 2 cut(s) 389, 801
PctI GAATGC 3 cut(s) 179, 483, 589
PdiI GCCGGC 1 cut(s) 331
PfeI GAWTC 4 cut(s) 206, 559, 664, 754
PfoI TCCNGGA 1 cut(s) 871
PkrI GCNGC 4 cut(s) 295, 324, 361, 919
PleI GAGTC 3 cut(s) 411, 448, 574
PpsI GAGTC 3 cut(s) 411, 448, 574
Psp6I CCWGG 2 cut(s) 778, 871
PspGI CCWGG 2 cut(s) 778, 871
PspN4I GGNNCC 4 cut(s) 286, 471, 490, 639
PspOMI GGGCCC 1 cut(s) 284
PspPI GGNCC 4 cut(s) 284, 285, 469, 489
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 611
SalI GTCGAC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 591, 761
SatI GCNGC 4 cut(s) 294, 323, 360, 918
Sau3AI GATC 2 cut(s) 611, 892
Sau96I GGNCC 4 cut(s) 284, 285, 469, 489
SchI GAGTC 3 cut(s) 412, 449, 575
ScrFI CCNGG 5 cut(s) 190, 288, 474, 780, 873
SduI GDGCHC 2 cut(s) 235, 288
SfaNI GCATC 1 cut(s) 904
Sfr274I CTCGAG 1 cut(s) 803
SinI GGWCC 2 cut(s) 469, 489
SlaI CTCGAG 1 cut(s) 803
SmlI CTYRAG 5 cut(s) 449, 530, 573, 590, 803
SmoI CTYRAG 5 cut(s) 449, 530, 573, 590, 803
Sse9I AATT 4 cut(s) 237, 268, 368, 758
SsiI CCGC 3 cut(s) 322, 359, 486
StyD4I CCNGG 5 cut(s) 188, 286, 472, 778, 871
TaiI ACGT 1 cut(s) 858
TaqI TCGA 6 cut(s) 235, 302, 315, 392, 653, 804
TaqII GACCGA 2 cut(s) 320, 832
TasI AATT 4 cut(s) 237, 268, 368, 758
TauI GCSGC 2 cut(s) 325, 362
TfiI GAWTC 4 cut(s) 206, 559, 664, 754
Tru1I TTAA 2 cut(s) 591, 761
Tru9I TTAA 2 cut(s) 591, 761
TscAI CASTG 3 cut(s) 68, 280, 450
TseFI GTSAC 1 cut(s) 441
TseI GCWGC 2 cut(s) 293, 917
Tsp45I GTSAC 1 cut(s) 441
TspDTI ATGAA 1 cut(s) 839
TspRI CASTG 3 cut(s) 68, 280, 450
Vha464I CTTAAG 1 cut(s) 590
VpaK11BI GGWCC 2 cut(s) 469, 489
XapI RAATTY 2 cut(s) 268, 368
XhoI CTCGAG 1 cut(s) 803
XmiI GTMKAC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.