FvH4_4g18951

Major allergen Pru ar

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006928
Physical Location & Seq
Reverse (-)
22714193 .. 22715298
1106 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18951.t1

Sequence Viewer

Length: 483 bp
ATGGGTGTGACAAGCATTTCACAGGAGTTCTTGTGCCCGATTGCCCCATCGCGCATGTTCAAGGCTTTGATCATAGACTCTAAGAACTTGATCCCAAAGCTGTTGCCCCAATTCATAGCAAGCGTTGAGGTAACTCAAGGAGATGGCGGAGCAGGAAGCATTGAACAAGTCAACTTCACAGAAGGTAGCCATTTCAAATACGTCAAGCACAGAATCGATGAACTCGACCAGGACAATTTTGTGTGCAAGTACACTATGATTGAGGGAGATGCATTAGGTGACAAGCTTGAGTCTATTGGCTATGAGGTCAAGTTTGAGGCAGCTAGTGATGGGAGCTCTATCTGCAAGATGACAAGTAATTACAACCCCATTGGAGAAGCTGAGGTCAAGGAAGAGGAGATTATAGCAGGAAAGAATAGTGCTATGGGGATTTACGAAGTAGTTGAAACCTTCCTCGTAGAGAACCCAAATGTTTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

161

Amino Acids

17.57

Weight (kDa)

4.51

Isoelectric Point (pI)

26.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 136 9.7e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 52
AciI CCGC 1 cut(s) 147
AclWI GGATC 1 cut(s) 85
AfaI GTAC 1 cut(s) 251
AgsI TTSAA 4 cut(s) 61, 164, 196, 446
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 1 cut(s) 460
AluBI AGCT 5 cut(s) 100, 286, 323, 336, 380
AluI AGCT 5 cut(s) 100, 286, 323, 336, 380
Alw21I GWGCWC 1 cut(s) 338
AlwI GGATC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 320
AspLEI GCGC 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 290
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 338
Bbv12I GWGCWC 1 cut(s) 338
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 332
BccI CCATC 3 cut(s) 55, 137, 323
BciT130I CCWGG 1 cut(s) 230
BclI TGATCA 1 cut(s) 69
BfaI CTAG 1 cut(s) 324
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 230
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 259
Bpu10I CCTNAGC 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 120, 308
Bsa29I ATCGAT 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
BseBI CCWGG 1 cut(s) 230
BseCI ATCGAT 1 cut(s) 216
BseMII CTCAG 1 cut(s) 372
BseRI GAGGAG 1 cut(s) 410
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 52
BshVI ATCGAT 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 38, 338
Bsp143I GATC 2 cut(s) 69, 90
BspACI CCGC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 373
BspDI ATCGAT 1 cut(s) 216
BspFNI CGCG 1 cut(s) 52
BspPI GGATC 1 cut(s) 85
BssMI GATC 2 cut(s) 69, 90
Bst2UI CCWGG 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 2 cut(s) 81, 381
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 2 cut(s) 72, 93
BstMBI GATC 2 cut(s) 69, 90
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 1 cut(s) 58
BstSCI CCNGG 1 cut(s) 228
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 332
Bsu15I ATCGAT 1 cut(s) 216
BsuTUI ATCGAT 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 1 cut(s) 250
CviAII CATG 1 cut(s) 55
CviJI RGCY 8 cut(s) 65, 100, 189, 286, 300, 323, 336, 380
CviKI_1 RGCY 8 cut(s) 65, 100, 189, 286, 300, 323, 336, 380
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 2 cut(s) 81, 381
DpnI GATC 2 cut(s) 71, 92
DpnII GATC 2 cut(s) 69, 90
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
EciI GGCGGA 1 cut(s) 162
Ecl136II GAGCTC 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 338
FaeI CATG 1 cut(s) 58
FaiI YATR 7 cut(s) 56, 74, 116, 257, 303, 404, 425
FatI CATG 1 cut(s) 54
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 321
FriOI GRGCYC 1 cut(s) 338
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 53
GluI GCNGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 54
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 77, 213, 290
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 2 cut(s) 172, 252
Hpy188III TCNNGA 1 cut(s) 480
Hpy8I GTNNAC 2 cut(s) 172, 252
HpyAV CCTTC 2 cut(s) 176, 460
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 246, 272, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 2 cut(s) 81, 381
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 52
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 2 cut(s) 69, 90
LmnI GCTCC 2 cut(s) 149, 333
LpnPI CCDG 5 cut(s) 8, 138, 215, 242, 393
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 1 cut(s) 259
MaeI CTAG 1 cut(s) 324
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 3 cut(s) 7, 130, 278
MalI GATC 2 cut(s) 71, 92
MboI GATC 2 cut(s) 69, 90
MboII GAAGA 1 cut(s) 404
MhlI GDGCHC 2 cut(s) 38, 338
MluCI AATT 3 cut(s) 110, 235, 358
MlyI GAGTC 2 cut(s) 71, 299
MnlI CCTC 7 cut(s) 121, 256, 298, 310, 376, 388, 464
Mph1103I ATGCAT 1 cut(s) 274
MspR9I CCNGG 1 cut(s) 230
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 342
NdeII GATC 2 cut(s) 69, 90
NlaIII CATG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 7, 278
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 58
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 213
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 2 cut(s) 71, 298
PpsI GAGTC 2 cut(s) 71, 298
Psp124BI GAGCTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 228
PspGI CCWGG 1 cut(s) 228
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SacI GAGCTC 1 cut(s) 338
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 2 cut(s) 69, 90
SchI GAGTC 2 cut(s) 71, 299
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 2 cut(s) 38, 338
SfaNI GCATC 1 cut(s) 259
SmlI CTYRAG 2 cut(s) 135, 287
SmoI CTYRAG 2 cut(s) 135, 287
Sse9I AATT 3 cut(s) 110, 235, 358
SsiI CCGC 1 cut(s) 147
SspMI CTAG 1 cut(s) 324
SstI GAGCTC 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 228
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 216, 225
TasI AATT 3 cut(s) 110, 235, 358
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 1 cut(s) 213
TseFI GTSAC 2 cut(s) 7, 278
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 2 cut(s) 7, 278
TspDTI ATGAA 2 cut(s) 103, 234
XceI RCATGY 1 cut(s) 58
XspI CTAG 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.