RchiOBHm_Chr4g0423861

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006928
Physical Location & Seq
Reverse (-)
49460975 .. 49462158
1184 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39319

Sequence Viewer

Length: 492 bp
ATGGGGATCACCAAAATCAGTCAGAAGTTTATTACTCAGGTAACTCCAGAAAGGATGTTCAAGGCCTTGATCCTTGATGCTCACAACCTCTGTCCCAAGCTCATGTTCTCATCGATTAAAAGTATTGAATTCCTTGAAGGTGAAGGAGAAGTTGGAACCATCAAGCAGATCAACTTCACTGAAGCTAGTCCTATGAGATATGCTAAACACAGGATTGATGCTCTGGACAAGGAGAATCTGATGTGCAGGTACACCTTCATTGAAAGTGATGCAGCAGAACATTTGCTGGACAAGCTTGAGTACATTACCTATGATGTGAGGTTTGAAGGGTATGGCAGGGGAGGGTGTATATGTCATTTGACAAGTGAATACAAAGCAAAAGATGGTACTCAGATCAAAGAAGAGGACATTGAGCTTGGCAAAGACAGAGCCATTGGGATGTATGAAGTCTTAGAGGCCTACCTTATGGCTCATCCCCGTGCATACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

163

Amino Acids

18.7

Weight (kDa)

5.58

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 5 - 155 3.3e-19 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 237
AclWI GGATC 2 cut(s) 14, 64
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 3 cut(s) 251, 302, 388
AgsI TTSAA 5 cut(s) 61, 128, 137, 263, 326
AjuI GAANNNNNNNTTGG 4 cut(s) 89, 121, 135, 167
AluBI AGCT 4 cut(s) 100, 185, 295, 415
AluI AGCT 4 cut(s) 100, 185, 295, 415
AlwI GGATC 2 cut(s) 14, 64
AoxI GGCC 2 cut(s) 63, 456
ApeKI GCWGC 1 cut(s) 272
ApoI RAATTY 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 152
BbvI GCAGC 1 cut(s) 284
BccI CCATC 2 cut(s) 167, 377
BfaI CTAG 1 cut(s) 186
BfuAI ACCTGC 1 cut(s) 237
BisI GCNGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 3 cut(s) 67, 208, 259
BpmI CTGGAG 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 113
BseCI ATCGAT 1 cut(s) 113
BseGI GGATG 3 cut(s) 60, 444, 472
BseMII CTCAG 2 cut(s) 50, 404
BseXI GCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 265
BshFI GGCC 2 cut(s) 65, 458
BshVI ATCGAT 1 cut(s) 113
BslFI GGGAC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 2 cut(s) 65, 458
Bsp143I GATC 4 cut(s) 6, 69, 168, 393
BspANI GGCC 2 cut(s) 65, 458
BspCNI CTCAG 2 cut(s) 49, 403
BspDI ATCGAT 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 2 cut(s) 14, 64
BssMI GATC 4 cut(s) 6, 69, 168, 393
Bst6I CTCTTC 1 cut(s) 396
BstDEI CTNAG 3 cut(s) 36, 390, 451
BstF5I GGATG 3 cut(s) 60, 444, 472
BstKTI GATC 4 cut(s) 9, 72, 171, 396
BstMBI GATC 4 cut(s) 6, 69, 168, 393
BstMWI GCNNNNNNNGC 1 cut(s) 292
BstV1I GCAGC 1 cut(s) 284
Bsu15I ATCGAT 1 cut(s) 113
BsuRI GGCC 2 cut(s) 65, 458
BsuTUI ATCGAT 1 cut(s) 113
BtsCI GGATG 3 cut(s) 60, 444, 472
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 1 cut(s) 237
ClaI ATCGAT 1 cut(s) 113
Csp6I GTAC 3 cut(s) 250, 301, 387
CviAII CATG 1 cut(s) 103
CviJI RGCY 8 cut(s) 65, 100, 185, 295, 415, 431, 458, 470
CviKI_1 RGCY 8 cut(s) 65, 100, 185, 295, 415, 431, 458, 470
CviQI GTAC 3 cut(s) 250, 301, 387
DdeI CTNAG 3 cut(s) 36, 390, 451
DpnI GATC 4 cut(s) 8, 71, 170, 395
DpnII GATC 4 cut(s) 6, 69, 168, 393
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
Eco147I AGGCCT 2 cut(s) 65, 458
Eco57I CTGAAG 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 128
FaeI CATG 1 cut(s) 106
FaqI GGGAC 1 cut(s) 78
FatI CATG 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 3 cut(s) 67, 451, 459
Fsp4HI GCNGC 1 cut(s) 273
FspBI CTAG 1 cut(s) 186
GluI GCNGC 1 cut(s) 273
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 2 cut(s) 65, 458
Hin1II CATG 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 235
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 3 cut(s) 24, 240, 393
Hpy188III TCNNGA 2 cut(s) 47, 224
Hpy8I GTNNAC 1 cut(s) 252
HpyAV CCTTC 4 cut(s) 131, 137, 265, 320
HpyCH4V TGCA 3 cut(s) 246, 272, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 292
HpyF3I CTNAG 3 cut(s) 36, 390, 451
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 4 cut(s) 6, 69, 168, 393
LpnPI CCDG 7 cut(s) 23, 60, 196, 209, 232, 272, 322
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 3 cut(s) 67, 208, 259
MaeI CTAG 1 cut(s) 186
MaeIII GTNAC 1 cut(s) 40
MalI GATC 4 cut(s) 8, 71, 170, 395
MboI GATC 4 cut(s) 6, 69, 168, 393
MboII GAAGA 1 cut(s) 413
MluCI AATT 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 5 cut(s) 98, 312, 335, 397, 448
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 2 cut(s) 437, 477
MwoI GCNNNNNNNGC 1 cut(s) 292
NdeII GATC 4 cut(s) 6, 69, 168, 393
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 1 cut(s) 157
PceI AGGCCT 2 cut(s) 65, 458
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 1 cut(s) 274
PspN4I GGNNCC 1 cut(s) 157
RsaI GTAC 3 cut(s) 251, 302, 388
RsaNI GTAC 3 cut(s) 250, 301, 387
RseI CAYNNNNRTG 2 cut(s) 437, 477
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 4 cut(s) 6, 69, 168, 393
SfaNI GCATC 3 cut(s) 67, 208, 259
SmiMI CAYNNNNRTG 2 cut(s) 437, 477
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 1 cut(s) 128
SseBI AGGCCT 2 cut(s) 65, 458
SspMI CTAG 1 cut(s) 186
StuI AGGCCT 2 cut(s) 65, 458
TaqI TCGA 1 cut(s) 113
TasI AATT 1 cut(s) 128
TatI WGTACW 1 cut(s) 300
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 184
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 247, 459
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 128
XspI CTAG 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.