RchiOBHm_Chr4g0423851

Major allergen Pru ar

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006928
Physical Location & Seq
Reverse (-)
49450619 .. 49451351
733 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39318

Sequence Viewer

Length: 624 bp
ATGATAAAAACTTTGAAAATTCTTTGTAAATTGGATTTCCATCTGTATATAGATGTACTGCCACTGGTTAGTTTCCACCGGTTCCTATATATAATCAAGGTTTGTCATCTAGATCCTCCTTTTACAATTAGTCTACATATCATGGGTGTGACAAGCGTTTCACAGGAGTTCTTGTGCCCGATTGCCCCATCGCGCATGTTCAAGGCTTTGATCATAGACTCCAAAAACTTGATCCCAAAGCTGTTGCCCCAATTCATAGCAAGCGTTGAAGTAACTCAAGGAGATGGCGGAGCAGGAAGCATTGAACAAGTCAACTTCACAGAAGGTAGCCATTTCAAATACGTCAAGCACAGAATCGATGAACTCGACCAGGACAATTTCGTGTGCAAGTACACTATGATTGAGGGAGATGCATTAGGGGACAAGCTTGAGTCTATTGCTTACGAGGTCAAGTTTGAGGCAGCTAGTGATGGGAGCTCTATATGCAAGATGACAAGTAATTACAACTCCATTGGAGAATTTGAGGTCAAAGAAGAGGAGATTTTAGCAGGAAAGAATAGTGCTATGGGAATTTACAAAGTCGTTGAAGCCTACCTCTTAGAGAACCCAAATGTTTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

207

Amino Acids

23.23

Weight (kDa)

5.24

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 48 - 202 8.5e-24 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 133
AccII CGCG 1 cut(s) 193
AciI CCGC 1 cut(s) 288
AclWI GGATC 2 cut(s) 107, 226
AcsI RAATTY 3 cut(s) 18, 518, 570
AfaI GTAC 2 cut(s) 57, 392
AgeI ACCGGT 1 cut(s) 78
AgsI TTSAA 6 cut(s) 16, 202, 269, 305, 337, 587
AjnI CCWGG 1 cut(s) 369
AjuI GAANNNNNNNTTGG 1 cut(s) 601
AluBI AGCT 4 cut(s) 241, 427, 464, 477
AluI AGCT 4 cut(s) 241, 427, 464, 477
Alw21I GWGCWC 1 cut(s) 479
AlwI GGATC 2 cut(s) 107, 226
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 3 cut(s) 18, 518, 570
AsiGI ACCGGT 1 cut(s) 78
AspLEI GCGC 1 cut(s) 195
BaeGI GKGCMC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 479
BbvI GCAGC 1 cut(s) 473
BccI CCATC 4 cut(s) 48, 196, 278, 464
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 1 cut(s) 210
BfaI CTAG 2 cut(s) 110, 465
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 1 cut(s) 371
BmiI GGNNCC 1 cut(s) 83
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 1 cut(s) 400
BpuEI CTTGAG 2 cut(s) 261, 449
Bsa29I ATCGAT 1 cut(s) 357
BsaBI GATNNNNATC 1 cut(s) 39
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 1 cut(s) 69
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 1 cut(s) 371
BseCI ATCGAT 1 cut(s) 357
BseJI GATNNNNATC 1 cut(s) 39
BseNI ACTGG 1 cut(s) 69
BseRI GAGGAG 1 cut(s) 551
BseSI GKGCMC 1 cut(s) 179
BseXI GCAGC 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 193
BshTI ACCGGT 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 479
BsiSI CCGG 1 cut(s) 79
BslFI GGGAC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 434
Bsp1286I GDGCHC 2 cut(s) 179, 479
Bsp143I GATC 3 cut(s) 112, 210, 231
BspACI CCGC 1 cut(s) 288
BspDI ATCGAT 1 cut(s) 357
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 83
BspPI GGATC 2 cut(s) 107, 226
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 78
BssMI GATC 3 cut(s) 112, 210, 231
Bst2UI CCWGG 1 cut(s) 371
Bst6I CTCTTC 1 cut(s) 528
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 598
BstFNI CGCG 1 cut(s) 193
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 3 cut(s) 115, 213, 234
BstMBI GATC 3 cut(s) 112, 210, 231
BstMWI GCNNNNNNNGC 1 cut(s) 483
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 179
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 473
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 357
BsuTUI ATCGAT 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 62
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 2 cut(s) 56, 391
CspAI ACCGGT 1 cut(s) 78
CviAII CATG 2 cut(s) 142, 196
CviJI RGCY 7 cut(s) 206, 241, 330, 427, 464, 477, 590
CviKI_1 RGCY 7 cut(s) 206, 241, 330, 427, 464, 477, 590
CviQI GTAC 2 cut(s) 56, 391
DdeI CTNAG 1 cut(s) 598
DpnI GATC 3 cut(s) 114, 212, 233
DpnII GATC 3 cut(s) 112, 210, 231
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
EciI GGCGGA 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 479
Eco53kI GAGCTC 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 477
EcoRII CCWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 479
FaeI CATG 2 cut(s) 145, 199
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 141, 195
FbaI TGATCA 1 cut(s) 210
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 1 cut(s) 462
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 2 cut(s) 110, 465
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 462
HapII CCGG 1 cut(s) 79
HhaI GCGC 1 cut(s) 195
Hin1II CATG 2 cut(s) 145, 199
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 3 cut(s) 218, 354, 431
HpaII CCGG 1 cut(s) 79
Hpy166II GTNNAC 3 cut(s) 134, 313, 393
Hpy188III TCNNGA 2 cut(s) 110, 621
Hpy8I GTNNAC 3 cut(s) 134, 313, 393
HpyAV CCTTC 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 3 cut(s) 387, 413, 486
HpyF10VI GCNNNNNNNGC 1 cut(s) 483
HpyF3I CTNAG 1 cut(s) 598
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 2 cut(s) 145, 199
HspAI GCGC 1 cut(s) 193
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 3 cut(s) 112, 210, 231
LmnI GCTCC 2 cut(s) 290, 474
LpnPI CCDG 7 cut(s) 50, 92, 149, 279, 356, 383, 534
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 110, 465
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 148, 271
MalI GATC 3 cut(s) 114, 212, 233
MboI GATC 3 cut(s) 112, 210, 231
MboII GAAGA 1 cut(s) 545
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 2 cut(s) 179, 479
MluCI AATT 8 cut(s) 18, 29, 126, 251, 376, 499, 518, 570
MlyI GAGTC 2 cut(s) 212, 440
MnlI CCTC 7 cut(s) 126, 397, 439, 451, 517, 529, 605
Mph1103I ATGCAT 1 cut(s) 415
MslI CAYNNNNRTG 1 cut(s) 146
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 483
NdeII GATC 3 cut(s) 112, 210, 231
NlaIII CATG 2 cut(s) 145, 199
NlaIV GGNNCC 1 cut(s) 83
NmuCI GTSAC 1 cut(s) 148
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 1 cut(s) 199
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 1 cut(s) 354
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 2 cut(s) 212, 439
PpsI GAGTC 2 cut(s) 212, 439
Psp124BI GAGCTC 1 cut(s) 479
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 1 cut(s) 83
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 57, 392
RsaNI GTAC 2 cut(s) 56, 391
RseI CAYNNNNRTG 1 cut(s) 146
SacI GAGCTC 1 cut(s) 479
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 3 cut(s) 112, 210, 231
SchI GAGTC 2 cut(s) 212, 440
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 2 cut(s) 179, 479
SfaNI GCATC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 2 cut(s) 276, 428
SmoI CTYRAG 2 cut(s) 276, 428
Sse9I AATT 8 cut(s) 18, 29, 126, 251, 376, 499, 518, 570
SsiI CCGC 1 cut(s) 288
SspMI CTAG 2 cut(s) 110, 465
SstI GAGCTC 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 369
TaiI ACGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 357, 366
TasI AATT 8 cut(s) 18, 29, 126, 251, 376, 499, 518, 570
TatI WGTACW 2 cut(s) 55, 390
TfiI GAWTC 1 cut(s) 354
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 244, 375
TspRI CASTG 1 cut(s) 69
XapI RAATTY 3 cut(s) 18, 518, 570
XbaI TCTAGA 1 cut(s) 109
XceI RCATGY 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 2 cut(s) 110, 465
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.