MD13G1161700.v1.1

Major allergen Pru ar

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006928
Physical Location & Seq
Forward (+)
12732001 .. 12733718
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1161700.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGTTCATGTTGTTTTTCTTTTTTCCCCGAAGTGCTTTTCCAGTTCTTCTTTCCTAGACTTTCTTGTTTCTAACTTAGCGACCAAAATACTTTGCAACTTTGAAAGGCATTCAAAGACTGCAGCTACAACCCTACCCTACAACCTAATTTTTTATGTTTTCCACGTTGATTACACCAACCTAGCTACATACACACGCATCTCAAACTTAAGCTCTATATATGCATGCTGCCAATGGTTTCCAACTACGGCTGCTATTACAACTAATCTACAAATTATGGGTGTGACAAGTATAACACAGGAGTTCCTCTGCCCAATCGCCCCGGTGCGCATGTTCAAGGCTTTGATCATAGACTCAAAAAATCTGATACCAAAGCTGTTGCCCCAGTTCATCGCAAGCGTCGATGTAACACAGGGAGATGGAGGAGCAGGAAGCATTGAACAAGTCAATTTCACGGAAGGTAGCCATTTCAGATACGTGAAGCACCGGATTGACGAACTTGACGTAGAGAATTTTGTGTGCAAGTACACTATGATTGAAGGGGATGCATTAGGTGAGAAGCTTGAGTCTATTGCTTATGAGGTGAAGTTTGAGGCAGCTAGTGATGGGAGCTCAATCTGCAAGATGACAAGTAAATACAACACCATTGGGGAGTTTGAGGTCAAGGAAGAGGAAATCATAGCAGGCAAGAACAGTGCTATCGGGATATACAAAGTTGTGGAAGCCTACCTCTTGGAGAACCCAAATGTTTATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

253

Amino Acids

28.16

Weight (kDa)

5.28

Isoelectric Point (pI)

26.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 93 - 246 7.8e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 329
AcsI RAATTY 1 cut(s) 511
AfaI GTAC 1 cut(s) 527
AflII CTTAAG 1 cut(s) 208
AgsI TTSAA 5 cut(s) 104, 114, 337, 440, 539
AjuI GAANNNNNNNTTGG 1 cut(s) 736
AluBI AGCT 7 cut(s) 125, 185, 213, 376, 562, 599, 612
AluI AGCT 7 cut(s) 125, 185, 213, 376, 562, 599, 612
Alw21I GWGCWC 1 cut(s) 614
ApeKI GCWGC 4 cut(s) 122, 228, 251, 596
ApoI RAATTY 1 cut(s) 511
AspLEI GCGC 1 cut(s) 330
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 566, 595
BanII GRGCYC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 4 cut(s) 134, 215, 238, 608
BccI CCATC 2 cut(s) 413, 599
BceAI ACGGC 1 cut(s) 264
BclI TGATCA 1 cut(s) 345
BcnI CCSGG 1 cut(s) 323
BfaI CTAG 3 cut(s) 56, 182, 600
BfmI CTRYAG 1 cut(s) 120
BfrI CTTAAG 1 cut(s) 208
BisI GCNGC 4 cut(s) 123, 229, 252, 597
BlsI GCNGC 4 cut(s) 124, 230, 253, 598
Bme1390I CCNGG 1 cut(s) 323
BmrFI CCNGG 1 cut(s) 323
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 207, 535
BmuI ACTGGG 1 cut(s) 379
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 478
BsaJI CCNNGG 1 cut(s) 321
BsaWI WCCGGW 1 cut(s) 486
Bse1I ACTGG 2 cut(s) 42, 385
BseDI CCNNGG 1 cut(s) 321
BseGI GGATG 1 cut(s) 550
BseNI ACTGG 2 cut(s) 42, 385
BseRI GAGGAG 1 cut(s) 438
BseXI GCAGC 4 cut(s) 134, 215, 238, 608
BsiHKAI GWGCWC 1 cut(s) 614
BsiSI CCGG 2 cut(s) 323, 487
BsmI GAATGC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 1 cut(s) 345
BspMAI CTGCAG 1 cut(s) 124
BspTI CTTAAG 1 cut(s) 208
BsrI ACTGG 2 cut(s) 42, 385
BssECI CCNNGG 1 cut(s) 321
BssMI GATC 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 695
Bst6I CTCTTC 1 cut(s) 663
BstAFI CTTAAG 1 cut(s) 208
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 3 cut(s) 226, 397, 685
BstDEI CTNAG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 550
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 1 cut(s) 348
BstMBI GATC 1 cut(s) 345
BstMWI GCNNNNNNNGC 1 cut(s) 618
BstNSI RCATGY 2 cut(s) 228, 334
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 4 cut(s) 134, 215, 238, 608
BtgZI GCGATG 1 cut(s) 376
BtsCI GGATG 1 cut(s) 550
BtsIMutI CAGTG 1 cut(s) 700
Cac8I GCNNGC 3 cut(s) 226, 397, 685
CfoI GCGC 1 cut(s) 330
CseI GACGC 1 cut(s) 388
Csp6I GTAC 1 cut(s) 526
CviAII CATG 3 cut(s) 8, 225, 331
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 1 cut(s) 76
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Ecl136II GAGCTC 1 cut(s) 612
Eco24I GRGCYC 1 cut(s) 614
Eco53kI GAGCTC 1 cut(s) 612
EcoICRI GAGCTC 1 cut(s) 612
EcoT22I ATGCAT 2 cut(s) 226, 550
EcoT38I GRGCYC 1 cut(s) 614
FaeI CATG 3 cut(s) 11, 228, 334
FatI CATG 3 cut(s) 7, 224, 330
FbaI TGATCA 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 123, 229, 252, 597
FokI GGATG 1 cut(s) 557
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 4 cut(s) 123, 229, 252, 597
FspAI RTGCGCAY 1 cut(s) 329
FspBI CTAG 3 cut(s) 56, 182, 600
FspI TGCGCA 1 cut(s) 329
GlaI GCGC 1 cut(s) 329
GluI GCNGC 4 cut(s) 123, 229, 252, 597
HapII CCGG 2 cut(s) 323, 487
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 330
Hin1II CATG 3 cut(s) 11, 228, 334
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 2 cut(s) 353, 566
HpaII CCGG 2 cut(s) 323, 487
HphI GGTGA 2 cut(s) 566, 595
Hpy166II GTNNAC 1 cut(s) 528
Hpy188I TCNGA 2 cut(s) 366, 473
Hpy188III TCNNGA 2 cut(s) 703, 756
Hpy8I GTNNAC 1 cut(s) 528
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 2 cut(s) 452, 533
HpyCH4III ACNGT 1 cut(s) 695
HpyCH4IV ACGT 3 cut(s) 165, 477, 504
HpyCH4V TGCA 6 cut(s) 96, 122, 224, 522, 548, 621
HpyF10VI GCNNNNNNNGC 1 cut(s) 618
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 3 cut(s) 165, 477, 504
Hsp92II CATG 3 cut(s) 11, 228, 334
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 345
Kzo9I GATC 1 cut(s) 345
LmnI GCTCC 2 cut(s) 425, 609
LpnPI CCDG 8 cut(s) 55, 284, 336, 398, 398, 414, 500, 669
Lsp1109I GCAGC 4 cut(s) 134, 215, 238, 608
LweI GCATC 2 cut(s) 207, 535
MaeI CTAG 3 cut(s) 56, 182, 600
MaeII ACGT 3 cut(s) 165, 477, 504
MaeIII GTNAC 2 cut(s) 283, 406
MalI GATC 1 cut(s) 347
MboI GATC 1 cut(s) 345
MboII GAAGA 2 cut(s) 39, 680
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 4 cut(s) 147, 273, 448, 511
MlyI GAGTC 2 cut(s) 347, 575
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 7 cut(s) 317, 416, 574, 586, 652, 664, 740
Mph1103I ATGCAT 2 cut(s) 226, 550
MseI TTAA 1 cut(s) 209
MspCI CTTAAG 1 cut(s) 208
MspI CCGG 2 cut(s) 323, 487
MspR9I CCNGG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 618
NciI CCSGG 1 cut(s) 323
NdeII GATC 1 cut(s) 345
NlaIII CATG 3 cut(s) 11, 228, 334
NmuCI GTSAC 1 cut(s) 283
NsbI TGCGCA 1 cut(s) 329
NsiI ATGCAT 2 cut(s) 226, 550
NspI RCATGY 2 cut(s) 228, 334
PaeI GCATGC 1 cut(s) 228
PcsI WCGNNNNNNNCGW 2 cut(s) 399, 501
PctI GAATGC 1 cut(s) 109
PkrI GCNGC 4 cut(s) 124, 230, 253, 598
PleI GAGTC 2 cut(s) 347, 574
PpsI GAGTC 2 cut(s) 347, 574
Ppu21I YACGTR 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 614
PstI CTGCAG 1 cut(s) 124
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SacI GAGCTC 1 cut(s) 614
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 4 cut(s) 123, 229, 252, 597
Sau3AI GATC 1 cut(s) 345
SchI GAGTC 2 cut(s) 347, 575
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 1 cut(s) 614
SfaNI GCATC 2 cut(s) 207, 535
SfcI CTRYAG 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 208, 563
SmoI CTYRAG 2 cut(s) 208, 563
SphI GCATGC 1 cut(s) 228
Sse9I AATT 4 cut(s) 147, 273, 448, 511
SspMI CTAG 3 cut(s) 56, 182, 600
SstI GAGCTC 1 cut(s) 614
StyD4I CCNGG 1 cut(s) 321
TaaI ACNGT 1 cut(s) 695
TaiI ACGT 3 cut(s) 168, 480, 507
TaqI TCGA 1 cut(s) 402
TasI AATT 4 cut(s) 147, 273, 448, 511
TatI WGTACW 1 cut(s) 525
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 4 cut(s) 122, 228, 251, 596
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 379
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 1 cut(s) 700
Vha464I CTTAAG 1 cut(s) 208
XapI RAATTY 1 cut(s) 511
XceI RCATGY 2 cut(s) 228, 334
XspI CTAG 3 cut(s) 56, 182, 600
Zsp2I ATGCAT 2 cut(s) 226, 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.