Rorug04G0191400

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006928
Physical Location & Seq
Forward (+)
33414838 .. 33416106
1269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0191400.1

Sequence Viewer

Length: 609 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAGCGGAGGAATTGGTCGTGGGTTGCTTCGTCTCCATCAAGACCACCTTGGGCGACGACTTCCAGGGCCAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTATATCAAGGAGTTGAGCTATTTGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGAGGAATCAGCTATCAGACAAGCAGAGGCAGAGTGTGAGAGGATCGGAGTTGGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAGTCCATATCTTCCCGAGTCTGTCAGTGGAGGAACTCCTGCTGCCAATGATCGGGTGAAGAAAGTGCTTGAGTTGGAGAAGACTTTTCCCTCAAGGGATAAGTCATTGTGGCTCAAGATAAATTGTTTAATCATGGTGGTAAAATTGATTAAATGCAAGTATGGTGGTATGGTGGTCTTGCTGTCAAGACGTAAGACCAAGCATGAAGTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

202

Amino Acids

22.34

Weight (kDa)

9.05

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AD PF09793 71 - 153 6e-26 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AciI CCGC 2 cut(s) 32, 121
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 2 cut(s) 97, 444
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 3 cut(s) 90, 96, 300
AluBI AGCT 3 cut(s) 165, 179, 248
AluI AGCT 3 cut(s) 165, 179, 248
Alw26I GTCTC 2 cut(s) 64, 230
AlwI GGATC 1 cut(s) 287
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 4 cut(s) 3, 94, 113, 141
ApeKI GCWGC 5 cut(s) 15, 23, 26, 29, 434
Asp700I GAANNNNTTC 1 cut(s) 314
AspS9I GGNCC 2 cut(s) 94, 113
AsuHPI GGTGA 2 cut(s) 286, 460
AvaI CYCGRG 1 cut(s) 407
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 189, 479
BbvI GCAGC 5 cut(s) 27, 35, 38, 41, 421
BccI CCATC 2 cut(s) 5, 71
BciT130I CCWGG 3 cut(s) 92, 98, 302
BcoDI GTCTC 2 cut(s) 64, 230
BisI GCNGC 5 cut(s) 16, 24, 27, 30, 435
BlsI GCNGC 5 cut(s) 17, 25, 28, 31, 436
Bme1390I CCNGG 3 cut(s) 92, 98, 302
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 94, 113
BmrFI CCNGG 3 cut(s) 92, 98, 302
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 2 cut(s) 189, 479
Bpu10I CCTNAGC 1 cut(s) 117
BpuEI CTTGAG 4 cut(s) 122, 469, 482, 491
BsaJI CCNNGG 4 cut(s) 6, 75, 91, 97
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 444
Bse1I ACTGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 302
BseDI CCNNGG 4 cut(s) 6, 75, 91, 97
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 2 cut(s) 97, 444
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 2 cut(s) 241, 320
BseNI ACTGG 1 cut(s) 341
BseXI GCAGC 5 cut(s) 27, 35, 38, 41, 421
BseYI CCCAGC 1 cut(s) 348
BshFI GGCC 4 cut(s) 5, 96, 115, 143
BsiHKCI CYCGRG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 2 cut(s) 97, 444
BsmAI GTCTC 2 cut(s) 64, 230
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 365
BsnI GGCC 4 cut(s) 5, 96, 115, 143
BsoBI CYCGRG 1 cut(s) 407
Bsp143I GATC 3 cut(s) 214, 279, 442
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 2 cut(s) 32, 121
BspANI GGCC 4 cut(s) 5, 96, 115, 143
BspCNI CTCAG 2 cut(s) 240, 319
BspPI GGATC 1 cut(s) 287
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 341
BssECI CCNNGG 4 cut(s) 6, 75, 91, 97
BssMI GATC 3 cut(s) 214, 279, 442
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2UI CCWGG 3 cut(s) 92, 98, 302
Bst4CI ACNGT 2 cut(s) 206, 361
BstDEI CTNAG 4 cut(s) 117, 227, 306, 330
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 3 cut(s) 217, 282, 445
BstMAI GTCTC 2 cut(s) 64, 230
BstMBI GATC 3 cut(s) 214, 279, 442
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 3 cut(s) 92, 98, 302
BstSCI CCNGG 3 cut(s) 90, 96, 300
BstV1I GCAGC 5 cut(s) 27, 35, 38, 41, 421
BstV2I GAAGAC 2 cut(s) 189, 479
BstXI CCANNNNNNTGG 1 cut(s) 348
BsuRI GGCC 4 cut(s) 5, 96, 115, 143
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 16
BtsIMutI CAGTG 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 94, 113
CseI GACGC 1 cut(s) 343
Csp6I GTAC 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 3 cut(s) 7, 526, 596
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 4 cut(s) 117, 227, 306, 330
DpnI GATC 3 cut(s) 216, 281, 444
DpnII GATC 3 cut(s) 214, 279, 442
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 2 cut(s) 6, 75
Eco57I CTGAAG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 407
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 3 cut(s) 90, 96, 300
EcoT14I CCWWGG 2 cut(s) 6, 75
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 3 cut(s) 10, 529, 599
FaiI YATR 8 cut(s) 8, 150, 365, 400, 527, 555, 563, 597
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 174, 206
FaqI GGGAC 1 cut(s) 365
FatI CATG 3 cut(s) 6, 525, 595
Fnu4HI GCNGC 5 cut(s) 16, 24, 27, 30, 435
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 5 cut(s) 16, 24, 27, 30, 435
GluI GCNGC 5 cut(s) 16, 24, 27, 30, 435
GsaI CCCAGC 1 cut(s) 352
HaeIII GGCC 4 cut(s) 5, 96, 115, 143
HgaI GACGC 1 cut(s) 343
Hin1II CATG 3 cut(s) 10, 529, 599
HinfI GANTC 2 cut(s) 242, 410
HphI GGTGA 2 cut(s) 286, 460
Hpy188I TCNGA 4 cut(s) 230, 254, 284, 309
Hpy188III TCNNGA 4 cut(s) 67, 407, 508, 579
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 2 cut(s) 206, 361
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 2 cut(s) 23, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 117, 227, 306, 330
HpySE526I ACGT 1 cut(s) 583
Hsp92II CATG 3 cut(s) 10, 529, 599
Kzo9I GATC 3 cut(s) 214, 279, 442
Lsp1109I GCAGC 5 cut(s) 27, 35, 38, 41, 421
LweI GCATC 1 cut(s) 310
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 2 cut(s) 206, 292
MalI GATC 3 cut(s) 216, 281, 444
MboI GATC 3 cut(s) 214, 279, 442
MboII GAAGA 4 cut(s) 194, 395, 463, 484
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 38, 514, 536
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 8 cut(s) 28, 104, 232, 256, 270, 373, 416, 493
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 198, 219, 521, 543
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 92, 98, 302
MvaI CCWGG 3 cut(s) 92, 98, 302
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 214, 279, 442
NlaIII CATG 3 cut(s) 10, 529, 599
NmuCI GTSAC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 5 cut(s) 17, 25, 28, 31, 436
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
Psp6I CCWGG 3 cut(s) 90, 96, 300
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 3 cut(s) 90, 96, 300
PspPI GGNCC 2 cut(s) 94, 113
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 4 cut(s) 198, 219, 521, 543
SatI GCNGC 5 cut(s) 16, 24, 27, 30, 435
Sau3AI GATC 3 cut(s) 214, 279, 442
Sau96I GGNCC 2 cut(s) 94, 113
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 3 cut(s) 92, 98, 302
SetI ASST 7 cut(s) 77, 167, 181, 213, 250, 384, 586
SfaNI GCATC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 4 cut(s) 137, 461, 484, 506
SmoI CTYRAG 4 cut(s) 137, 461, 484, 506
Sse9I AATT 3 cut(s) 38, 514, 536
SsiI CCGC 2 cut(s) 32, 121
StyD4I CCNGG 3 cut(s) 90, 96, 300
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 2 cut(s) 206, 361
TaiI ACGT 1 cut(s) 586
TaqI TCGA 1 cut(s) 318
TasI AATT 3 cut(s) 38, 514, 536
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 4 cut(s) 198, 219, 521, 543
Tru9I TTAA 4 cut(s) 198, 219, 521, 543
TscAI CASTG 1 cut(s) 424
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 5 cut(s) 15, 23, 26, 29, 434
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 1 cut(s) 389
TspRI CASTG 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.