MD16G1161000.v1.1

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006928
Physical Location & Seq
Forward (+)
13123320 .. 13124395
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1161000.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGGTGTCACCAAGATCAGCCAGAAGTTTGTGACTCAGGTGACGCCACAGAGGATGTTCAACGCCTTGATCTTGGATGCCCACAACATCTGCCCCAAGCTCATGTTCTCATCAATTAAAAGTATTGAATTCCTTAGCGGTTCAGGAGAAGTTGGAACCATCAAACAGATCAACTTCACTGAAGCTAGTCCTATGAAATATGCTAAGCACAGGATTGATGCTTTGGACAAGGAGGCACTCAGCTGCACCTACACCTTCATCGAAAGCGATGCAACGGATCACTTGTTGGACAAGCTTGAATACATAACATATGATGTCAAGTTTGAGGGGTATGGAAGAGGAGGATGCATCTGTCATTTGACCAGCACATACAAAGCCAAAGATGATATACAGATCAAAGAAGAAGACATTGAGCTTGGCAAGGATAGAGCTATTGGGATGTATGAAGTTTTGGAAGCCTATCTCATGGCGCACCCTCGCGCCTACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

164

Amino Acids

18.43

Weight (kDa)

5.72

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 155 1.9e-17 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 480
AciI CCGC 1 cut(s) 138
AclWI GGATC 1 cut(s) 285
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 44
AgsI TTSAA 3 cut(s) 61, 128, 299
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 6 cut(s) 100, 185, 243, 295, 415, 431
AluI AGCT 6 cut(s) 100, 185, 243, 295, 415, 431
AlwI GGATC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 128
AspLEI GCGC 2 cut(s) 472, 482
AsuHPI GGTGA 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 230
BccI CCATC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BfaI CTAG 1 cut(s) 186
BisI GCNGC 1 cut(s) 244
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 5 cut(s) 67, 208, 259, 335, 357
BpiI GAAGAC 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 134
Bpu1102I GCTNAGC 1 cut(s) 204
BsaHI GRCGYC 1 cut(s) 44
BseGI GGATG 4 cut(s) 60, 82, 350, 444
BseMII CTCAG 2 cut(s) 50, 253
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 230
BsgI GTGCAG 1 cut(s) 229
Bsh1236I CGCG 1 cut(s) 480
Bsp143I GATC 5 cut(s) 15, 69, 168, 277, 393
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 49, 252
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 157
BspPI GGATC 1 cut(s) 285
BssMI GATC 5 cut(s) 15, 69, 168, 277, 393
BssNI GRCGYC 1 cut(s) 44
Bst6I CTCTTC 1 cut(s) 331
BstACI GRCGYC 1 cut(s) 44
BstDEI CTNAG 4 cut(s) 36, 134, 204, 239
BstF5I GGATG 4 cut(s) 60, 82, 350, 444
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 2 cut(s) 472, 482
BstKTI GATC 5 cut(s) 18, 72, 171, 280, 396
BstMBI GATC 5 cut(s) 15, 69, 168, 277, 393
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 1 cut(s) 230
BstV2I GAAGAC 1 cut(s) 411
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 4 cut(s) 60, 82, 350, 444
BtsIMutI CAGTG 1 cut(s) 177
CfoI GCGC 2 cut(s) 472, 482
CseI GACGC 1 cut(s) 52
CviAII CATG 2 cut(s) 103, 466
CviJI RGCY 9 cut(s) 21, 100, 185, 243, 295, 377, 415, 431, 458
CviKI_1 RGCY 9 cut(s) 21, 100, 185, 243, 295, 377, 415, 431, 458
DdeI CTNAG 4 cut(s) 36, 134, 204, 239
DpnI GATC 5 cut(s) 17, 71, 170, 279, 395
DpnII GATC 5 cut(s) 15, 69, 168, 277, 393
Eam1104I CTCTTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 350
FaeI CATG 2 cut(s) 106, 469
FatI CATG 2 cut(s) 102, 465
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 244
FokI GGATG 4 cut(s) 67, 89, 357, 451
Fsp4HI GCNGC 1 cut(s) 244
FspBI CTAG 1 cut(s) 186
GlaI GCGC 2 cut(s) 471, 481
GluI GCNGC 1 cut(s) 244
HgaI GACGC 1 cut(s) 52
HhaI GCGC 2 cut(s) 472, 482
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 2 cut(s) 106, 469
Hin6I GCGC 2 cut(s) 470, 480
HinP1I GCGC 2 cut(s) 470, 480
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 34
HphI GGTGA 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 491
Hpy188III TCNNGA 1 cut(s) 144
HpyAV CCTTC 1 cut(s) 265
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 3 cut(s) 246, 272, 348
HpyF3I CTNAG 4 cut(s) 36, 134, 204, 239
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 106, 469
HspAI GCGC 2 cut(s) 470, 480
Kzo9I GATC 5 cut(s) 15, 69, 168, 277, 393
LpnPI CCDG 5 cut(s) 23, 35, 129, 196, 376
Lsp1109I GCAGC 1 cut(s) 230
LweI GCATC 5 cut(s) 67, 208, 259, 335, 357
MaeI CTAG 1 cut(s) 186
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 3 cut(s) 7, 31, 40
MalI GATC 5 cut(s) 17, 71, 170, 279, 395
MboI GATC 5 cut(s) 15, 69, 168, 277, 393
MboII GAAGA 3 cut(s) 348, 413, 416
MluCI AATT 2 cut(s) 114, 128
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 2 cut(s) 133, 267
MnlI CCTC 6 cut(s) 45, 226, 319, 332, 335, 486
Mph1103I ATGCAT 1 cut(s) 350
MseI TTAA 1 cut(s) 117
MspA1I CMGCKG 1 cut(s) 243
MvnI CGCG 1 cut(s) 480
NdeI CATATG 1 cut(s) 310
NdeII GATC 5 cut(s) 15, 69, 168, 277, 393
NlaIII CATG 2 cut(s) 106, 469
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 3 cut(s) 7, 31, 40
NsiI ATGCAT 1 cut(s) 350
PkrI GCNGC 1 cut(s) 245
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 243
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 1 cut(s) 244
Sau3AI GATC 5 cut(s) 15, 69, 168, 277, 393
SchI GAGTC 1 cut(s) 28
SfaNI GCATC 5 cut(s) 67, 208, 259, 335, 357
Sse9I AATT 2 cut(s) 114, 128
SsiI CCGC 1 cut(s) 138
SspMI CTAG 1 cut(s) 186
TaiI ACGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 261
TasI AATT 2 cut(s) 114, 128
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 3 cut(s) 7, 31, 40
TseI GCWGC 1 cut(s) 243
Tsp45I GTSAC 3 cut(s) 7, 31, 40
TspDTI ATGAA 3 cut(s) 209, 247, 459
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 128
XspI CTAG 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.