MD13G1161600.v1.1

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006928
Physical Location & Seq
Forward (+)
12730972 .. 12731999
1028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1161600.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGGAATCACCAAGATCAGCCAGAAGTTCGTGACTCAGGTGACGCCACAGAGGATGTTCAACGCCTTGATCTTGGACTCCCACAACCTCTGCCCCAAGCTCATGTTCTCATCAATTAAAAGTATTGAATTCCTTAGCGGTTCAGGAGAAGTTGGAACCATCAAACAGATCAACTTCACTGAAGCTAGTCCTATGAAATATGTTAAGCACAGGATTGATGCTTTGGACAAGGAGGCCCTCAGCTGCAGCTACACATTCATCGAAAGCGACGCGTCGGATCACTTGATGGACAAGCTTGAATACATAACTTATGATGTCAAGTTTGAGGGGTATGGAAGAGGAGGATGCATCTGTCATTTGACCAGCACATACAAAGCCAAAGACGATATTCAGATCAAAGAAGAAGACATTGAGCTTGGCAAGGATAGAGCTATTGGGATGTATGAAGTGTTGGAAGCCTACCTCATGGCACACCCTCGCGCCTACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

164

Amino Acids

18.5

Weight (kDa)

5.72

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 4 - 155 4.3e-17 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 272, 480
AciI CCGC 1 cut(s) 138
AclWI GGATC 1 cut(s) 285
AcsI RAATTY 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 3 cut(s) 61, 128, 299
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 7 cut(s) 100, 185, 243, 249, 295, 415, 431
AluI AGCT 7 cut(s) 100, 185, 243, 249, 295, 415, 431
AlwI GGATC 1 cut(s) 285
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 243, 246
ApoI RAATTY 1 cut(s) 128
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 411
BbvCI CCTCAGC 1 cut(s) 239
BbvI GCAGC 2 cut(s) 230, 258
BccI CCATC 2 cut(s) 167, 280
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 244
BisI GCNGC 2 cut(s) 244, 247
BlsI GCNGC 2 cut(s) 245, 248
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 3 cut(s) 208, 335, 357
BpiI GAAGAC 1 cut(s) 411
Bpu10I CCTNAGC 2 cut(s) 134, 239
BsaHI GRCGYC 1 cut(s) 44
BseGI GGATG 3 cut(s) 60, 350, 444
BseMII CTCAG 2 cut(s) 50, 253
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 230, 258
Bsh1236I CGCG 2 cut(s) 272, 480
BshFI GGCC 1 cut(s) 236
BsnI GGCC 1 cut(s) 236
Bsp143I GATC 5 cut(s) 15, 69, 168, 277, 393
BspACI CCGC 1 cut(s) 138
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 2 cut(s) 49, 252
BspFNI CGCG 2 cut(s) 272, 480
BspLI GGNNCC 1 cut(s) 157
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 1 cut(s) 285
BssMI GATC 5 cut(s) 15, 69, 168, 277, 393
BssNI GRCGYC 1 cut(s) 44
Bst6I CTCTTC 1 cut(s) 331
BstACI GRCGYC 1 cut(s) 44
BstDEI CTNAG 3 cut(s) 36, 134, 239
BstF5I GGATG 3 cut(s) 60, 350, 444
BstFNI CGCG 2 cut(s) 272, 480
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 5 cut(s) 18, 72, 171, 280, 396
BstMBI GATC 5 cut(s) 15, 69, 168, 277, 393
BstSFI CTRYAG 1 cut(s) 244
BstUI CGCG 2 cut(s) 272, 480
BstV1I GCAGC 2 cut(s) 230, 258
BstV2I GAAGAC 1 cut(s) 411
BsuRI GGCC 1 cut(s) 236
BtsCI GGATG 3 cut(s) 60, 350, 444
BtsIMutI CAGTG 1 cut(s) 177
CfoI GCGC 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 3 cut(s) 52, 261, 278
CviAII CATG 2 cut(s) 103, 466
DdeI CTNAG 3 cut(s) 36, 134, 239
DpnI GATC 5 cut(s) 17, 71, 170, 279, 395
DpnII GATC 5 cut(s) 15, 69, 168, 277, 393
Eam1104I CTCTTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 128
EcoT22I ATGCAT 1 cut(s) 350
FaeI CATG 2 cut(s) 106, 469
FaiI YATR 9 cut(s) 104, 194, 201, 305, 312, 333, 370, 444, 467
FatI CATG 2 cut(s) 102, 465
Fnu4HI GCNGC 2 cut(s) 244, 247
FokI GGATG 3 cut(s) 67, 357, 451
Fsp4HI GCNGC 2 cut(s) 244, 247
FspBI CTAG 1 cut(s) 186
GlaI GCGC 1 cut(s) 481
GluI GCNGC 2 cut(s) 244, 247
HaeIII GGCC 1 cut(s) 236
HgaI GACGC 3 cut(s) 52, 261, 278
HhaI GCGC 1 cut(s) 482
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 2 cut(s) 106, 469
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 3 cut(s) 6, 34, 77
HphI GGTGA 1 cut(s) 52
Hpy188I TCNGA 3 cut(s) 277, 393, 491
Hpy188III TCNNGA 2 cut(s) 31, 144
Hpy99I CGWCG 2 cut(s) 272, 277
HpyCH4V TGCA 2 cut(s) 246, 348
HpyF3I CTNAG 3 cut(s) 36, 134, 239
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 2 cut(s) 106, 469
HspAI GCGC 1 cut(s) 480
Kzo9I GATC 5 cut(s) 15, 69, 168, 277, 393
LpnPI CCDG 5 cut(s) 23, 35, 129, 196, 376
Lsp1109I GCAGC 2 cut(s) 230, 258
LweI GCATC 3 cut(s) 208, 335, 357
MaeI CTAG 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 31, 40
MalI GATC 5 cut(s) 17, 71, 170, 279, 395
MboI GATC 5 cut(s) 15, 69, 168, 277, 393
MboII GAAGA 3 cut(s) 348, 413, 416
MluCI AATT 2 cut(s) 114, 128
MluI ACGCGT 1 cut(s) 270
MlyI GAGTC 2 cut(s) 28, 71
MmeI TCCRAC 3 cut(s) 133, 255, 432
MnlI CCTC 9 cut(s) 45, 98, 226, 248, 319, 332, 335, 473, 486
Mph1103I ATGCAT 1 cut(s) 350
MseI TTAA 2 cut(s) 117, 204
MspA1I CMGCKG 1 cut(s) 243
MvnI CGCG 2 cut(s) 272, 480
NdeII GATC 5 cut(s) 15, 69, 168, 277, 393
NlaIII CATG 2 cut(s) 106, 469
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 2 cut(s) 31, 40
NsiI ATGCAT 1 cut(s) 350
PfeI GAWTC 1 cut(s) 6
PkrI GCNGC 2 cut(s) 245, 248
PleI GAGTC 2 cut(s) 28, 71
PpsI GAGTC 2 cut(s) 28, 71
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 235
PstI CTGCAG 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 243
SaqAI TTAA 2 cut(s) 117, 204
SatI GCNGC 2 cut(s) 244, 247
Sau3AI GATC 5 cut(s) 15, 69, 168, 277, 393
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 28, 71
SfaNI GCATC 3 cut(s) 208, 335, 357
SfcI CTRYAG 1 cut(s) 244
Sse9I AATT 2 cut(s) 114, 128
SsiI CCGC 1 cut(s) 138
SspMI CTAG 1 cut(s) 186
TaqI TCGA 1 cut(s) 261
TasI AATT 2 cut(s) 114, 128
TfiI GAWTC 1 cut(s) 6
Tru1I TTAA 2 cut(s) 117, 204
Tru9I TTAA 2 cut(s) 117, 204
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 2 cut(s) 31, 40
TseI GCWGC 2 cut(s) 243, 246
Tsp45I GTSAC 2 cut(s) 31, 40
TspDTI ATGAA 3 cut(s) 209, 247, 459
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 128
XspI CTAG 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.