FvH4_4g19330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011771
Physical Location & Seq
Reverse (-)
23011667 .. 23012572
906 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19330.t1

Sequence Viewer

Length: 531 bp
ATGGCATTAACAAAAATCCAACTCTTAGTTTTAGCTGCAGCTTTGATCAACATCTCTCTTTTCTGCAAAATCGGTTCTGCAGATGAAGATGGGCCTGAAACAGGTGACGTACCGCAAACAGGCGGTGGCCAAGAATTTGATCCAGCTCAAATTGTTGCCAAAGCCTTGCTGTGTTTCAATGATAACTATGTATACAGCAGCTGCCAAGCGTCTTATAGGTTGAATGAGAGTGGAGATCTGAATGTTCCTAAAGAGAAAACCGACGAGTTCTGCAATGGCGCTTGCCTCACAGAGACACACCTTGTGCTCAACTGTGTTGATCACATTTTGAACAACTTCATCTTCTACAACAAGGCCACTGTAAGAGATGTTAGAGATACGATTCATGCCGGATGTGGATATGGCCCCGAAAGAGGTGACTTCAACGTGGCTGAGCATATGGCTGTGACGAGTGAGGCACACAAGGCTGCAAATTACCAGATTATTGTTGGGTTTATCTTGATGGTTTTGGGAAATGGATCACTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

177

Amino Acids

19.07

Weight (kDa)

4.74

Isoelectric Point (pI)

29.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 48 - 147 5.9e-46 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 192
AciI CCGC 2 cut(s) 113, 123
AclWI GGATC 2 cut(s) 134, 526
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 1 cut(s) 134
AdeI CACNNNGTG 1 cut(s) 304
AfaI GTAC 1 cut(s) 111
AfiI CCNNNNNNNGG 3 cut(s) 101, 119, 413
AgsI TTSAA 4 cut(s) 178, 223, 331, 424
AluBI AGCT 4 cut(s) 35, 41, 146, 201
AluI AGCT 4 cut(s) 35, 41, 146, 201
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 2 cut(s) 134, 526
AlwNI CAGNNNCTG 1 cut(s) 201
AoxI GGCC 4 cut(s) 92, 127, 354, 403
ApeKI GCWGC 5 cut(s) 35, 38, 198, 201, 467
ApoI RAATTY 1 cut(s) 134
Asp700I GAANNNNTTC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 92, 404
AsuHPI GGTGA 2 cut(s) 116, 428
BalI TGGCCA 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 5 cut(s) 22, 50, 188, 210, 454
BccI CCATC 2 cut(s) 83, 496
BclI TGATCA 2 cut(s) 45, 319
BcoDI GTCTC 1 cut(s) 287
BfmI CTRYAG 2 cut(s) 36, 78
BfoI RGCGCY 1 cut(s) 282
BglII AGATCT 1 cut(s) 235
BisI GCNGC 5 cut(s) 36, 39, 199, 202, 468
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 5 cut(s) 37, 40, 200, 203, 469
BmgT120I GGNCC 2 cut(s) 92, 404
BmiI GGNNCC 1 cut(s) 406
Bpu1102I GCTNAGC 1 cut(s) 432
BsaBI GATNNNNATC 1 cut(s) 50
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 119, 413
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 1 cut(s) 50
BseGI GGATG 1 cut(s) 398
BseJI GATNNNNATC 1 cut(s) 50
BseLI CCNNNNNNNGG 3 cut(s) 101, 119, 413
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 423
BseXI GCAGC 5 cut(s) 22, 50, 188, 210, 454
BshFI GGCC 4 cut(s) 94, 129, 356, 405
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 3 cut(s) 101, 119, 413
BsmAI GTCTC 1 cut(s) 287
BsnI GGCC 4 cut(s) 94, 129, 356, 405
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 5 cut(s) 45, 139, 235, 319, 518
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 2 cut(s) 113, 123
BspANI GGCC 4 cut(s) 94, 129, 356, 405
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 2 cut(s) 40, 82
BspPI GGATC 2 cut(s) 134, 526
BsrDI GCAATG 1 cut(s) 280
BssMI GATC 5 cut(s) 45, 139, 235, 319, 518
BssNAI GTATAC 1 cut(s) 193
Bst1107I GTATAC 1 cut(s) 193
Bst4CI ACNGT 3 cut(s) 314, 361, 525
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 2 cut(s) 25, 432
BstENI CCTNNNNNAGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 5 cut(s) 48, 142, 238, 322, 521
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 5 cut(s) 45, 139, 235, 319, 518
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstSFI CTRYAG 2 cut(s) 36, 78
BstV1I GCAGC 5 cut(s) 22, 50, 188, 210, 454
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BstZ17I GTATAC 1 cut(s) 193
BsuRI GGCC 4 cut(s) 94, 129, 356, 405
BtsCI GGATG 1 cut(s) 398
BtsIMutI CAGTG 2 cut(s) 357, 521
Cac8I GCNNGC 1 cut(s) 283
CaiI CAGNNNCTG 1 cut(s) 201
CfoI GCGC 1 cut(s) 281
Cfr13I GGNCC 2 cut(s) 92, 404
CseI GACGC 1 cut(s) 198
Csp6I GTAC 1 cut(s) 110
CviAII CATG 1 cut(s) 386
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 2 cut(s) 25, 432
DpnI GATC 5 cut(s) 47, 141, 237, 321, 520
DpnII GATC 5 cut(s) 45, 139, 235, 319, 518
DraIII CACNNNGTG 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 127
EcoNI CCTNNNNNAGG 1 cut(s) 99
FaeI CATG 1 cut(s) 389
FaiI YATR 7 cut(s) 189, 193, 216, 387, 402, 438, 440
FatI CATG 1 cut(s) 385
FauNDI CATATG 1 cut(s) 438
FbaI TGATCA 2 cut(s) 45, 319
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 5 cut(s) 36, 39, 199, 202, 468
FokI GGATG 1 cut(s) 405
Fsp4HI GCNGC 5 cut(s) 36, 39, 199, 202, 468
GlaI GCGC 1 cut(s) 280
GluI GCNGC 5 cut(s) 36, 39, 199, 202, 468
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 4 cut(s) 94, 129, 356, 405
HapII CCGG 1 cut(s) 390
HgaI GACGC 1 cut(s) 198
HhaI GCGC 1 cut(s) 281
Hin1II CATG 1 cut(s) 389
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 1 cut(s) 382
HpaII CCGG 1 cut(s) 390
HphI GGTGA 2 cut(s) 116, 428
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 240, 530
Hpy188III TCNNGA 1 cut(s) 499
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 266
HpyCH4III ACNGT 3 cut(s) 314, 361, 525
HpyCH4IV ACGT 2 cut(s) 108, 426
HpyCH4V TGCA 5 cut(s) 38, 66, 80, 273, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 2 cut(s) 25, 432
HpySE526I ACGT 2 cut(s) 108, 426
Hsp92II CATG 1 cut(s) 389
HspAI GCGC 1 cut(s) 279
Ksp22I TGATCA 2 cut(s) 45, 319
Kzo9I GATC 5 cut(s) 45, 139, 235, 319, 518
LpnPI CCDG 6 cut(s) 87, 105, 108, 156, 403, 491
Lsp1109I GCAGC 5 cut(s) 22, 50, 188, 210, 454
MaeII ACGT 2 cut(s) 108, 426
MaeIII GTNAC 3 cut(s) 104, 416, 445
MalI GATC 5 cut(s) 47, 141, 237, 321, 520
MboI GATC 5 cut(s) 45, 139, 235, 319, 518
MboII GAAGA 2 cut(s) 98, 334
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 3 cut(s) 134, 150, 472
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 3 cut(s) 296, 407, 448
Mox20I TGGCCA 1 cut(s) 129
MroXI GAANNNNTTC 1 cut(s) 335
MscI TGGCCA 1 cut(s) 129
MseI TTAA 1 cut(s) 8
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 464
NdeI CATATG 1 cut(s) 438
NdeII GATC 5 cut(s) 45, 139, 235, 319, 518
NlaIII CATG 1 cut(s) 389
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 3 cut(s) 104, 416, 445
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 1 cut(s) 382
PkrI GCNGC 5 cut(s) 37, 40, 200, 203, 469
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 2 cut(s) 92, 404
PstI CTGCAG 2 cut(s) 40, 82
PstNI CAGNNNCTG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 201
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 5 cut(s) 36, 39, 199, 202, 468
Sau3AI GATC 5 cut(s) 45, 139, 235, 319, 518
Sau96I GGNCC 2 cut(s) 92, 404
SduI GDGCHC 1 cut(s) 309
SfcI CTRYAG 2 cut(s) 36, 78
Sse9I AATT 3 cut(s) 134, 150, 472
SsiI CCGC 2 cut(s) 113, 123
TaaI ACNGT 3 cut(s) 314, 361, 525
TaiI ACGT 2 cut(s) 111, 429
TasI AATT 3 cut(s) 134, 150, 472
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 364, 528
TseFI GTSAC 3 cut(s) 104, 416, 445
TseI GCWGC 5 cut(s) 35, 38, 198, 201, 467
Tsp45I GTSAC 3 cut(s) 104, 416, 445
TspDTI ATGAA 3 cut(s) 99, 328, 374
TspRI CASTG 2 cut(s) 364, 528
XagI CCTNNNNNAGG 1 cut(s) 99
XapI RAATTY 1 cut(s) 134
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.