Rroxscaffold_5G00366300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011771
Physical Location & Seq
Reverse (-)
48138740 .. 48139885
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00366300.1

Sequence Viewer

Length: 534 bp
ATGGCATTTACAAAAACCCAACTCTTAGTTTTAGCTGCAGCTTTGATCTACATCTCTCTTTCCTGCTGCAAAATGGGTTCTGCAGATGAAGATGGGCCTGAAACAGGTAATCTACCACAAACAGGTGGTGCTGGGGAATTCGATCCGGCTCAAATTGTTGCCAAAGCCTTGCTGTGTTTCAATAACAACTATGTATACAGCAGCTGCGAACAGTCTTTTAGATTGAATGAGAGTGGAGATCTGAATGTTCCAAAAGACAAAACCGACGAGTTCTGCGGTGGTGCTTGCCTCACAGAGACACACCTTGTGCTCAACTGTGTTGATCACATTTTGACCAACTTCGTCTTCTACAACAAGGCCACTATAAGAGATGTTAGAGATACTGTTCAGGCCGGATGTGGATCTGGCCCCGAAAGAGGTAACTTCAACGTGGAGGAGCATATGGCTGAGACGAGCAAGGCACACAGGCCTGCAACATACCAGATTCTTGTTGGGCTTGTTTTGATGGTTTTGGGAAATGGATTACTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

177

Amino Acids

19.12

Weight (kDa)

4.87

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 49 - 148 2.8e-44 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 195
AciI CCGC 1 cut(s) 276
AclWI GGATC 2 cut(s) 137, 409
AcsI RAATTY 1 cut(s) 137
AdeI CACNNNGTG 1 cut(s) 307
AfiI CCNNNNNNNGG 3 cut(s) 104, 122, 416
AgsI TTSAA 3 cut(s) 181, 226, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 3 cut(s) 35, 41, 204
AluI AGCT 3 cut(s) 35, 41, 204
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 2 cut(s) 290, 443
AlwI GGATC 2 cut(s) 137, 409
AlwNI CAGNNNCTG 1 cut(s) 204
AoxI GGCC 5 cut(s) 95, 357, 390, 406, 467
ApeKI GCWGC 5 cut(s) 35, 38, 66, 201, 204
ApoI RAATTY 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 95, 407
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 22, 50, 53, 191, 213
BccI CCATC 2 cut(s) 86, 499
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 2 cut(s) 290, 443
BfmI CTRYAG 2 cut(s) 36, 81
BglII AGATCT 1 cut(s) 238
BisI GCNGC 5 cut(s) 36, 39, 67, 202, 205
BlsI GCNGC 5 cut(s) 37, 40, 68, 203, 206
BmgT120I GGNCC 2 cut(s) 95, 407
BmiI GGNNCC 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 337
BsaBI GATNNNNATC 2 cut(s) 50, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 122, 416
Bse8I GATNNNNATC 2 cut(s) 50, 400
BseGI GGATG 1 cut(s) 401
BseJI GATNNNNATC 2 cut(s) 50, 400
BseLI CCNNNNNNNGG 3 cut(s) 104, 122, 416
BseMII CTCAG 1 cut(s) 438
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 5 cut(s) 22, 50, 53, 191, 213
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 5 cut(s) 97, 359, 392, 408, 469
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 2 cut(s) 146, 393
BslI CCNNNNNNNGG 3 cut(s) 104, 122, 416
BsmAI GTCTC 2 cut(s) 290, 443
BsmBI CGTCTC 1 cut(s) 443
BsnI GGCC 5 cut(s) 97, 359, 392, 408, 469
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 5 cut(s) 45, 142, 238, 322, 401
BspACI CCGC 1 cut(s) 276
BspANI GGCC 5 cut(s) 97, 359, 392, 408, 469
BspCNI CTCAG 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 409
BspMAI CTGCAG 2 cut(s) 40, 85
BspPI GGATC 2 cut(s) 137, 409
BssMI GATC 5 cut(s) 45, 142, 238, 322, 401
BssNAI GTATAC 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 196
Bst4CI ACNGT 4 cut(s) 213, 317, 385, 528
BstC8I GCNNGC 2 cut(s) 286, 471
BstDEI CTNAG 2 cut(s) 25, 447
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 401
BstKTI GATC 5 cut(s) 48, 145, 241, 325, 404
BstMAI GTCTC 2 cut(s) 290, 443
BstMBI GATC 5 cut(s) 45, 142, 238, 322, 401
BstSFI CTRYAG 2 cut(s) 36, 81
BstV1I GCAGC 5 cut(s) 22, 50, 53, 191, 213
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 2 cut(s) 238, 401
BstYI RGATCY 2 cut(s) 238, 401
BstZ17I GTATAC 1 cut(s) 196
BsuRI GGCC 5 cut(s) 97, 359, 392, 408, 469
BtsCI GGATG 1 cut(s) 401
Cac8I GCNNGC 2 cut(s) 286, 471
CaiI CAGNNNCTG 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 95, 407
DdeI CTNAG 2 cut(s) 25, 447
DpnI GATC 5 cut(s) 47, 144, 240, 324, 403
DpnII GATC 5 cut(s) 45, 142, 238, 322, 401
DraIII CACNNNGTG 1 cut(s) 307
Eco147I AGGCCT 1 cut(s) 469
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 137
Esp3I CGTCTC 1 cut(s) 443
FaiI YATR 6 cut(s) 192, 196, 365, 441, 443, 478
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 36, 39, 67, 202, 205
FokI GGATG 1 cut(s) 408
Fsp4HI GCNGC 5 cut(s) 36, 39, 67, 202, 205
GluI GCNGC 5 cut(s) 36, 39, 67, 202, 205
GsaI CCCAGC 1 cut(s) 135
HaeIII GGCC 5 cut(s) 97, 359, 392, 408, 469
HapII CCGG 2 cut(s) 146, 393
HinfI GANTC 1 cut(s) 484
HpaII CCGG 2 cut(s) 146, 393
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 243, 533
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 269
HpyCH4III ACNGT 4 cut(s) 213, 317, 385, 528
HpyCH4IV ACGT 1 cut(s) 429
HpyCH4V TGCA 4 cut(s) 38, 69, 83, 473
HpyF3I CTNAG 2 cut(s) 25, 447
HpySE526I ACGT 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 5 cut(s) 45, 142, 238, 322, 401
LmnI GCTCC 1 cut(s) 436
Lsp1109I GCAGC 5 cut(s) 22, 50, 53, 191, 213
MaeII ACGT 1 cut(s) 429
MaeIII GTNAC 1 cut(s) 419
MalI GATC 5 cut(s) 47, 144, 240, 324, 403
MboI GATC 5 cut(s) 45, 142, 238, 322, 401
MboII GAAGA 2 cut(s) 101, 337
MflI RGATCY 2 cut(s) 238, 401
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 2 cut(s) 137, 153
MnlI CCTC 3 cut(s) 299, 410, 427
MspA1I CMGCKG 1 cut(s) 204
MspI CCGG 2 cut(s) 146, 393
NdeI CATATG 1 cut(s) 441
NdeII GATC 5 cut(s) 45, 142, 238, 322, 401
NlaIV GGNNCC 1 cut(s) 409
PceI AGGCCT 1 cut(s) 469
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 5 cut(s) 37, 40, 68, 203, 206
PspFI CCCAGC 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 2 cut(s) 95, 407
PstI CTGCAG 2 cut(s) 40, 85
PstNI CAGNNNCTG 1 cut(s) 204
PsuI RGATCY 2 cut(s) 238, 401
PvuII CAGCTG 1 cut(s) 204
SatI GCNGC 5 cut(s) 36, 39, 67, 202, 205
Sau3AI GATC 5 cut(s) 45, 142, 238, 322, 401
Sau96I GGNCC 2 cut(s) 95, 407
SduI GDGCHC 1 cut(s) 312
SetI ASST 8 cut(s) 37, 43, 109, 127, 206, 306, 421, 432
SfcI CTRYAG 2 cut(s) 36, 81
Sse9I AATT 2 cut(s) 137, 153
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 1 cut(s) 276
StuI AGGCCT 1 cut(s) 469
TaaI ACNGT 4 cut(s) 213, 317, 385, 528
TaiI ACGT 1 cut(s) 432
TaqI TCGA 1 cut(s) 141
TasI AATT 2 cut(s) 137, 153
TfiI GAWTC 1 cut(s) 484
TseI GCWGC 5 cut(s) 35, 38, 66, 201, 204
TspDTI ATGAA 1 cut(s) 102
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.