MD16G1158500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011771
Physical Location & Seq
Forward (+)
12701021 .. 12702402
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1158500.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGCATTAACGATGAACCAACTCTCAGTTTTAGCTGCAGCTTTCATCTGCATCTCTCTTTTCTGCTGCAGAACAGGGATGGCTGATGATTATACACCTGAAAGAGGTTATGAACCAGAAAAAGGCACTGAACTCGAGGATCCAACTCAAATTGTAGCCAAAGCCTTGCTGTGTTTCAATGATAAATATGTTTACAGCAGCTGTGAAGAGTCTTACAGATTGAATGAGGGTGGAAATCTGAAGGTACCTGCAGAAAAAACTGATGAATACTGCAATGGTCCTTGCTTGACGGAGACACACCTTGTGCTCGATTGTCTTGAAAAAATCATGACCAACTTCCTGTTTTACAACAAGGCCACGATTCAAGACGTTCGAGACACGATTCATGCCGGATGTGGCCACGGCGAAGAAAGAGGTAAATTCGATGTGGCTGAGCACTTCAGAGCTGAAGGAAGCAATGCATACAAGGCTGCAAACCAGATTCTAATTGGGATAGTGTTGATGATTGTGGGCACTGGATTACTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

177

Amino Acids

19.51

Weight (kDa)

4.81

Isoelectric Point (pI)

18.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 48 - 147 1e-45 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 244
AclWI GGATC 2 cut(s) 134, 147
AcoI YGGCCR 1 cut(s) 397
AcsI RAATTY 1 cut(s) 419
AcuI CTGAAG 3 cut(s) 260, 424, 468
AdeI CACNNNGTG 1 cut(s) 304
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 104, 122
AgsI TTSAA 4 cut(s) 178, 223, 320, 365
AluBI AGCT 4 cut(s) 35, 41, 201, 446
AluI AGCT 4 cut(s) 35, 41, 201, 446
Alw21I GWGCWC 2 cut(s) 309, 438
Alw26I GTCTC 2 cut(s) 287, 369
AlwI GGATC 2 cut(s) 134, 147
AlwNI CAGNNNCTG 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 354, 397
ApeKI GCWGC 5 cut(s) 35, 38, 66, 198, 470
ApoI RAATTY 1 cut(s) 419
Asp718I GGTACC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 515
BalI TGGCCA 1 cut(s) 399
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 1 cut(s) 244
Bbv12I GWGCWC 2 cut(s) 309, 438
BbvI GCAGC 5 cut(s) 22, 50, 53, 210, 457
BccI CCATC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BcoDI GTCTC 2 cut(s) 287, 369
BfmI CTRYAG 3 cut(s) 36, 67, 249
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 5 cut(s) 36, 39, 67, 199, 471
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 5 cut(s) 37, 40, 68, 200, 472
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 141, 246
BmsI GCATC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 400
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 122
Bse1I ACTGG 1 cut(s) 520
Bse3DI GCAATG 2 cut(s) 280, 463
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 2 cut(s) 84, 398
BseLI CCNNNNNNNGG 2 cut(s) 104, 122
BseMI GCAATG 2 cut(s) 280, 463
BseMII CTCAG 2 cut(s) 39, 423
BseNI ACTGG 1 cut(s) 520
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 5 cut(s) 22, 50, 53, 210, 457
BshFI GGCC 2 cut(s) 356, 399
BshNI GGYRCC 1 cut(s) 244
BsiHKAI GWGCWC 2 cut(s) 309, 438
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 2 cut(s) 104, 122
BsmAI GTCTC 2 cut(s) 287, 369
BsnI GGCC 2 cut(s) 356, 399
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 3 cut(s) 309, 438, 515
Bsp143I GATC 1 cut(s) 139
Bsp1720I GCTNAGC 1 cut(s) 432
BspANI GGCC 2 cut(s) 356, 399
BspCNI CTCAG 2 cut(s) 38, 424
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 2 cut(s) 141, 246
BspMAI CTGCAG 3 cut(s) 40, 71, 253
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 2 cut(s) 134, 147
BspT107I GGYRCC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 280, 463
BsrI ACTGG 1 cut(s) 520
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 1 cut(s) 139
Bst4CI ACNGT 1 cut(s) 525
Bst6I CTCTTC 1 cut(s) 201
BstDEI CTNAG 2 cut(s) 25, 432
BstDSI CCRYGG 1 cut(s) 400
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 2 cut(s) 84, 398
BstKTI GATC 1 cut(s) 142
BstMAI GTCTC 2 cut(s) 287, 369
BstMBI GATC 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstSFI CTRYAG 3 cut(s) 36, 67, 249
BstSLI GKGCMC 1 cut(s) 515
BstV1I GCAGC 5 cut(s) 22, 50, 53, 210, 457
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 2 cut(s) 356, 399
BtgI CCRYGG 1 cut(s) 400
BtsCI GGATG 2 cut(s) 84, 398
BtsIMutI CAGTG 2 cut(s) 126, 513
BveI ACCTGC 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 201
CciI TCATGA 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 245
CviAII CATG 2 cut(s) 328, 386
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 2 cut(s) 25, 432
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
DraIII CACNNNGTG 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 397
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 3 cut(s) 260, 424, 468
Eco88I CYCGRG 1 cut(s) 134
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoT22I ATGCAT 1 cut(s) 463
FaeI CATG 2 cut(s) 331, 389
FaiI YATR 6 cut(s) 93, 111, 189, 329, 387, 463
FatI CATG 2 cut(s) 327, 385
Fnu4HI GCNGC 5 cut(s) 36, 39, 67, 199, 471
FokI GGATG 2 cut(s) 91, 405
Fsp4HI GCNGC 5 cut(s) 36, 39, 67, 199, 471
GluI GCNGC 5 cut(s) 36, 39, 67, 199, 471
HaeIII GGCC 2 cut(s) 356, 399
HapII CCGG 1 cut(s) 390
Hin1II CATG 2 cut(s) 331, 389
HinfI GANTC 4 cut(s) 209, 361, 382, 481
HpaII CCGG 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 2 cut(s) 240, 443
Hpy188III TCNNGA 4 cut(s) 317, 328, 365, 374
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 2 cut(s) 235, 443
HpyCH4III ACNGT 1 cut(s) 525
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 7 cut(s) 38, 51, 69, 251, 273, 461, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 2 cut(s) 25, 432
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 2 cut(s) 331, 389
KpnI GGTACC 1 cut(s) 248
Kzo9I GATC 1 cut(s) 139
LpnPI CCDG 8 cut(s) 60, 111, 129, 261, 353, 403, 491, 501
Lsp1109I GCAGC 5 cut(s) 22, 50, 53, 210, 457
LweI GCATC 1 cut(s) 60
MaeII ACGT 1 cut(s) 369
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 2 cut(s) 218, 419
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 3 cut(s) 309, 438, 515
MlsI TGGCCA 1 cut(s) 399
MluCI AATT 3 cut(s) 150, 419, 486
MluNI TGGCCA 1 cut(s) 399
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 167
MnlI CCTC 4 cut(s) 98, 130, 220, 407
Mox20I TGGCCA 1 cut(s) 399
Mph1103I ATGCAT 1 cut(s) 463
MscI TGGCCA 1 cut(s) 399
MseI TTAA 1 cut(s) 8
Msp20I TGGCCA 1 cut(s) 399
MspA1I CMGCKG 1 cut(s) 201
MspI CCGG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 467
NdeII GATC 1 cut(s) 139
NlaIII CATG 2 cut(s) 331, 389
NlaIV GGNNCC 2 cut(s) 141, 246
NsiI ATGCAT 1 cut(s) 463
PaeR7I CTCGAG 1 cut(s) 134
PagI TCATGA 1 cut(s) 327
PfeI GAWTC 3 cut(s) 361, 382, 481
PkrI GCNGC 5 cut(s) 37, 40, 68, 200, 472
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 141, 246
PspPI GGNCC 1 cut(s) 278
PspXI VCTCGAGB 1 cut(s) 134
PstI CTGCAG 3 cut(s) 40, 71, 253
PstNI CAGNNNCTG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 201
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 5 cut(s) 36, 39, 67, 199, 471
Sau3AI GATC 1 cut(s) 139
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 1 cut(s) 218
SduI GDGCHC 3 cut(s) 309, 438, 515
SfaNI GCATC 1 cut(s) 60
SfcI CTRYAG 3 cut(s) 36, 67, 249
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 3 cut(s) 150, 419, 486
TaaI ACNGT 1 cut(s) 525
TaiI ACGT 1 cut(s) 372
TaqI TCGA 4 cut(s) 135, 309, 373, 423
TasI AATT 3 cut(s) 150, 419, 486
TfiI GAWTC 3 cut(s) 361, 382, 481
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 133, 520
TseI GCWGC 5 cut(s) 35, 38, 66, 198, 470
TspDTI ATGAA 5 cut(s) 29, 34, 126, 279, 374
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 133, 520
VpaK11BI GGWCC 1 cut(s) 278
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 1 cut(s) 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.