RLG00000007482

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011771
Physical Location & Seq
Forward (+)
17391389 .. 17392331
943 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007482

Sequence Viewer

Length: 552 bp
ATGGCATTAACAAAAACCCAACTCTTAGTTTTAGCTGCAGCTTTGATATACATCTCTCTTTCCTGCTGCAAAATGGGTTCCGCAGATGAAGATGGGCCTGAAACAGGTAATGTACCACAAACAGGTAATGTACCACAAACAGGTGGTGCTGGGGAATTCGATCCGGCTCAAATTGTTGCCAAAGCCTTGCTGTGTTTCAATAACAACTATGTATACAGCAGCTGCGAACAGTCTTTTAGATTAAATGAGAGTGGAGGTCTGAATGTTCCAAAAGACAAAACCGACGAGTTCTGCAATGGTGCTTGCCTCACAGAGACACACCTTGTGCTCAACTGTGTTGATCACATTTTGGCCAACTTCGTCTTCTACAACAAGGCCACTATAAGAGATGTTAGAGATACTGTTCAGGCCGGATGTGGATCTGGCCCCGAAAGAGGCAACTTCAACGTGGAGGAGCATATGGCTGAGACGAGCAAGGCACACAAGCCTGCAACTTACCAGATTCTTGTTGGGCTTGTTTTGATGGTTTTGGGAAATGGATTAGTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

184

Amino Acids

19.59

Weight (kDa)

4.98

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 55 - 154 2.9e-44 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 213
AciI CCGC 1 cut(s) 81
AclWI GGATC 2 cut(s) 155, 427
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 1 cut(s) 155
AdeI CACNNNGTG 1 cut(s) 325
AfaI GTAC 2 cut(s) 114, 132
AfiI CCNNNNNNNGG 4 cut(s) 104, 122, 140, 434
AgsI TTSAA 2 cut(s) 199, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AluBI AGCT 3 cut(s) 35, 41, 222
AluI AGCT 3 cut(s) 35, 41, 222
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 2 cut(s) 308, 461
AlwI GGATC 2 cut(s) 155, 427
AlwNI CAGNNNCTG 1 cut(s) 222
AoxI GGCC 5 cut(s) 95, 351, 375, 408, 424
ApeKI GCWGC 5 cut(s) 35, 38, 66, 219, 222
ApoI RAATTY 1 cut(s) 155
AspS9I GGNCC 2 cut(s) 95, 425
BalI TGGCCA 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 5 cut(s) 22, 50, 53, 209, 231
BccI CCATC 2 cut(s) 86, 517
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 2 cut(s) 308, 461
BfmI CTRYAG 1 cut(s) 36
BisI GCNGC 5 cut(s) 36, 39, 67, 220, 223
BlsI GCNGC 5 cut(s) 37, 40, 68, 221, 224
BmgT120I GGNCC 2 cut(s) 95, 425
BmiI GGNNCC 2 cut(s) 79, 427
BpiI GAAGAC 1 cut(s) 355
BsaBI GATNNNNATC 2 cut(s) 50, 418
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 122, 140, 434
Bse3DI GCAATG 1 cut(s) 301
Bse8I GATNNNNATC 2 cut(s) 50, 418
BseGI GGATG 1 cut(s) 419
BseJI GATNNNNATC 2 cut(s) 50, 418
BseLI CCNNNNNNNGG 4 cut(s) 104, 122, 140, 434
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 456
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 5 cut(s) 22, 50, 53, 209, 231
BseYI CCCAGC 1 cut(s) 149
BshFI GGCC 5 cut(s) 97, 353, 377, 410, 426
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 2 cut(s) 164, 411
BslI CCNNNNNNNGG 4 cut(s) 104, 122, 140, 434
BsmAI GTCTC 2 cut(s) 308, 461
BsmBI CGTCTC 1 cut(s) 461
BsnI GGCC 5 cut(s) 97, 353, 377, 410, 426
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 3 cut(s) 160, 340, 419
BspACI CCGC 1 cut(s) 81
BspANI GGCC 5 cut(s) 97, 353, 377, 410, 426
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 79, 427
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 2 cut(s) 155, 427
BsrDI GCAATG 1 cut(s) 301
BssMI GATC 3 cut(s) 160, 340, 419
BssNAI GTATAC 1 cut(s) 214
Bst1107I GTATAC 1 cut(s) 214
Bst4CI ACNGT 3 cut(s) 231, 335, 403
BstC8I GCNNGC 2 cut(s) 304, 489
BstDEI CTNAG 2 cut(s) 25, 465
BstENI CCTNNNNNAGG 1 cut(s) 102
BstF5I GGATG 1 cut(s) 419
BstKTI GATC 3 cut(s) 163, 343, 422
BstMAI GTCTC 2 cut(s) 308, 461
BstMBI GATC 3 cut(s) 160, 340, 419
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 22, 50, 53, 209, 231
BstV2I GAAGAC 1 cut(s) 355
BstX2I RGATCY 1 cut(s) 419
BstYI RGATCY 1 cut(s) 419
BstZ17I GTATAC 1 cut(s) 214
BsuRI GGCC 5 cut(s) 97, 353, 377, 410, 426
BtsCI GGATG 1 cut(s) 419
Cac8I GCNNGC 2 cut(s) 304, 489
CaiI CAGNNNCTG 1 cut(s) 222
Cfr13I GGNCC 2 cut(s) 95, 425
Csp6I GTAC 2 cut(s) 113, 131
CviQI GTAC 2 cut(s) 113, 131
DdeI CTNAG 2 cut(s) 25, 465
DpnI GATC 3 cut(s) 162, 342, 421
DpnII GATC 3 cut(s) 160, 340, 419
DraIII CACNNNGTG 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 102
EcoRI GAATTC 1 cut(s) 155
Esp3I CGTCTC 1 cut(s) 461
FaiI YATR 6 cut(s) 49, 210, 214, 383, 459, 461
FauNDI CATATG 1 cut(s) 459
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 36, 39, 67, 220, 223
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 5 cut(s) 36, 39, 67, 220, 223
GluI GCNGC 5 cut(s) 36, 39, 67, 220, 223
GsaI CCCAGC 1 cut(s) 153
HaeIII GGCC 5 cut(s) 97, 353, 377, 410, 426
HapII CCGG 2 cut(s) 164, 411
HinfI GANTC 1 cut(s) 502
HpaII CCGG 2 cut(s) 164, 411
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 261, 551
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 287
HpyCH4III ACNGT 3 cut(s) 231, 335, 403
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 4 cut(s) 38, 69, 294, 491
HpyF3I CTNAG 2 cut(s) 25, 465
HpySE526I ACGT 1 cut(s) 447
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 3 cut(s) 160, 340, 419
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 5 cut(s) 22, 50, 53, 209, 231
MaeII ACGT 1 cut(s) 447
MalI GATC 3 cut(s) 162, 342, 421
MboI GATC 3 cut(s) 160, 340, 419
MboII GAAGA 2 cut(s) 101, 355
MflI RGATCY 1 cut(s) 419
MhlI GDGCHC 1 cut(s) 330
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 2 cut(s) 155, 171
MluNI TGGCCA 1 cut(s) 353
MnlI CCTC 4 cut(s) 248, 317, 428, 445
Mox20I TGGCCA 1 cut(s) 353
MscI TGGCCA 1 cut(s) 353
MseI TTAA 2 cut(s) 8, 242
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 222
MspI CCGG 2 cut(s) 164, 411
NdeI CATATG 1 cut(s) 459
NdeII GATC 3 cut(s) 160, 340, 419
NlaIV GGNNCC 2 cut(s) 79, 427
PfeI GAWTC 1 cut(s) 502
PkrI GCNGC 5 cut(s) 37, 40, 68, 221, 224
PspFI CCCAGC 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 79, 427
PspPI GGNCC 2 cut(s) 95, 425
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 1 cut(s) 419
PvuII CAGCTG 1 cut(s) 222
RsaI GTAC 2 cut(s) 114, 132
RsaNI GTAC 2 cut(s) 113, 131
SaqAI TTAA 2 cut(s) 8, 242
SatI GCNGC 5 cut(s) 36, 39, 67, 220, 223
Sau3AI GATC 3 cut(s) 160, 340, 419
Sau96I GGNCC 2 cut(s) 95, 425
SduI GDGCHC 1 cut(s) 330
SetI ASST 9 cut(s) 37, 43, 109, 127, 145, 224, 259, 324, 450
SfcI CTRYAG 1 cut(s) 36
Sse9I AATT 2 cut(s) 155, 171
SsiI CCGC 1 cut(s) 81
TaaI ACNGT 3 cut(s) 231, 335, 403
TaiI ACGT 1 cut(s) 450
TaqI TCGA 1 cut(s) 159
TasI AATT 2 cut(s) 155, 171
TfiI GAWTC 1 cut(s) 502
Tru1I TTAA 2 cut(s) 8, 242
Tru9I TTAA 2 cut(s) 8, 242
TseI GCWGC 5 cut(s) 35, 38, 66, 219, 222
TspDTI ATGAA 1 cut(s) 102
XagI CCTNNNNNAGG 1 cut(s) 102
XapI RAATTY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.