Rh4AG253000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011771
Physical Location & Seq
Reverse (-)
56762889 .. 56763891
1003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG253000.1

Sequence Viewer

Length: 609 bp
ATGGTTGGCATAATTAAGGCTACTCTGCTATACATTTTGCTCCAAGGTGTAGCCATTTTCTCTTCCATATCAGTCATGGCATTAACAAAAACCCAACTCTTAGTTTTAGCTGCAGCTTTGATCTACATCTCTCTTTCCTGCTGCAAAATGGGTTCCGCAGATGAAGATGGGCCTGAAACAGGTAATGTACCACAAACAGGTGGTGCTGGGGAATTCGATCCGGCTCAAATTGTTGCCAAAGCCTTGCTGTGTTTCAATAACAACTATGTATACAGCAGCTGCGAACAGTCTTTTAGATTGAATGAGAGTGGAGATCTGAATGTTCCAAAAGACAAAACCGACGAGTTCTGCAATGGTGCTTGCCTCACAGAGACACACCTTGTGCTCAACTGTGTTGATCACATTTTGGTCAACTTCCTCTTCTACAACAAGGCCACTATAAGAGATGTTAGAGATACTGTTCAGGCCGGATGTGGATCTGGCCCCGAAAGAGGCAACTTCAACGTGGAGGAGCATATGGCTGAGACGAGCAAGGCACGCAAGCCTGCAACTTACCAGATTCTTGTTGGGCTTGTTTTGATGGTTTTGGGAAATGGATTACTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

202

Amino Acids

21.76

Weight (kDa)

5.01

Isoelectric Point (pI)

32.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 74 - 173 4.5e-44 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 270
AciI CCGC 1 cut(s) 156
AclWI GGATC 2 cut(s) 212, 484
AcsI RAATTY 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 382
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 3 cut(s) 179, 197, 491
AgsI TTSAA 3 cut(s) 256, 301, 502
AluBI AGCT 3 cut(s) 110, 116, 279
AluI AGCT 3 cut(s) 110, 116, 279
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 2 cut(s) 365, 518
AlwI GGATC 2 cut(s) 212, 484
AlwNI CAGNNNCTG 1 cut(s) 279
AoxI GGCC 4 cut(s) 170, 432, 465, 481
ApeKI GCWGC 5 cut(s) 110, 113, 141, 276, 279
ApoI RAATTY 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 170, 482
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 5 cut(s) 97, 125, 128, 266, 288
BccI CCATC 2 cut(s) 161, 574
BclI TGATCA 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 365, 518
BfmI CTRYAG 1 cut(s) 111
BglII AGATCT 1 cut(s) 313
BisI GCNGC 5 cut(s) 111, 114, 142, 277, 280
BlsI GCNGC 5 cut(s) 112, 115, 143, 278, 281
BmgT120I GGNCC 2 cut(s) 170, 482
BmiI GGNNCC 2 cut(s) 154, 484
BsaBI GATNNNNATC 2 cut(s) 125, 475
BsaJI CCNNGG 1 cut(s) 43
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 197, 491
Bse3DI GCAATG 1 cut(s) 358
Bse8I GATNNNNATC 2 cut(s) 125, 475
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 476
BseJI GATNNNNATC 2 cut(s) 125, 475
BseLI CCNNNNNNNGG 3 cut(s) 179, 197, 491
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 524
BseXI GCAGC 5 cut(s) 97, 125, 128, 266, 288
BseYI CCCAGC 1 cut(s) 206
BshFI GGCC 4 cut(s) 172, 434, 467, 483
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 2 cut(s) 221, 468
BslI CCNNNNNNNGG 3 cut(s) 179, 197, 491
BsmAI GTCTC 2 cut(s) 365, 518
BsmBI CGTCTC 1 cut(s) 518
BsnI GGCC 4 cut(s) 172, 434, 467, 483
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 5 cut(s) 120, 217, 313, 397, 476
BspACI CCGC 1 cut(s) 156
BspANI GGCC 4 cut(s) 172, 434, 467, 483
BspCNI CTCAG 1 cut(s) 514
BspLI GGNNCC 2 cut(s) 154, 484
BspMAI CTGCAG 1 cut(s) 115
BspPI GGATC 2 cut(s) 212, 484
BsrDI GCAATG 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 5 cut(s) 120, 217, 313, 397, 476
BssNAI GTATAC 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 43
Bst1107I GTATAC 1 cut(s) 271
Bst4CI ACNGT 4 cut(s) 288, 392, 460, 603
Bst6I CTCTTC 2 cut(s) 67, 425
BstC8I GCNNGC 4 cut(s) 361, 538, 542, 546
BstDEI CTNAG 2 cut(s) 100, 522
BstENI CCTNNNNNAGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 476
BstKTI GATC 5 cut(s) 123, 220, 316, 400, 479
BstMAI GTCTC 2 cut(s) 365, 518
BstMBI GATC 5 cut(s) 120, 217, 313, 397, 476
BstMWI GCNNNNNNNGC 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 111
BstV1I GCAGC 5 cut(s) 97, 125, 128, 266, 288
BstX2I RGATCY 2 cut(s) 313, 476
BstYI RGATCY 2 cut(s) 313, 476
BstZ17I GTATAC 1 cut(s) 271
BsuRI GGCC 4 cut(s) 172, 434, 467, 483
BtsCI GGATG 1 cut(s) 476
Cac8I GCNNGC 4 cut(s) 361, 538, 542, 546
CaiI CAGNNNCTG 1 cut(s) 279
Cfr13I GGNCC 2 cut(s) 170, 482
Csp6I GTAC 1 cut(s) 188
CviAII CATG 1 cut(s) 76
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 2 cut(s) 100, 522
DpnI GATC 5 cut(s) 122, 219, 315, 399, 478
DpnII GATC 5 cut(s) 120, 217, 313, 397, 476
DraIII CACNNNGTG 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 67, 425
EarI CTCTTC 2 cut(s) 67, 425
Eco130I CCWWGG 1 cut(s) 43
EcoNI CCTNNNNNAGG 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
Esp3I CGTCTC 1 cut(s) 518
FaeI CATG 1 cut(s) 79
FaiI YATR 9 cut(s) 11, 31, 68, 77, 267, 271, 440, 516, 518
FatI CATG 1 cut(s) 75
FauNDI CATATG 1 cut(s) 516
FbaI TGATCA 1 cut(s) 397
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 111, 114, 142, 277, 280
FokI GGATG 1 cut(s) 483
Fsp4HI GCNGC 5 cut(s) 111, 114, 142, 277, 280
GluI GCNGC 5 cut(s) 111, 114, 142, 277, 280
GsaI CCCAGC 1 cut(s) 210
HaeIII GGCC 4 cut(s) 172, 434, 467, 483
HapII CCGG 2 cut(s) 221, 468
Hin1II CATG 1 cut(s) 79
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 1 cut(s) 559
HpaII CCGG 2 cut(s) 221, 468
Hpy166II GTNNAC 2 cut(s) 271, 412
Hpy188I TCNGA 2 cut(s) 318, 608
Hpy8I GTNNAC 2 cut(s) 271, 412
Hpy99I CGWCG 1 cut(s) 344
HpyCH4III ACNGT 4 cut(s) 288, 392, 460, 603
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 4 cut(s) 113, 144, 351, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 537
HpyF3I CTNAG 2 cut(s) 100, 522
HpySE526I ACGT 1 cut(s) 504
Hsp92II CATG 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 5 cut(s) 120, 217, 313, 397, 476
LmnI GCTCC 2 cut(s) 45, 511
Lsp1109I GCAGC 5 cut(s) 97, 125, 128, 266, 288
MaeII ACGT 1 cut(s) 504
MalI GATC 5 cut(s) 122, 219, 315, 399, 478
MboI GATC 5 cut(s) 120, 217, 313, 397, 476
MboII GAAGA 3 cut(s) 54, 176, 412
MflI RGATCY 2 cut(s) 313, 476
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 3 cut(s) 12, 212, 228
MnlI CCTC 4 cut(s) 374, 428, 485, 502
MseI TTAA 2 cut(s) 15, 83
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 2 cut(s) 221, 468
MwoI GCNNNNNNNGC 1 cut(s) 537
NdeI CATATG 1 cut(s) 516
NdeII GATC 5 cut(s) 120, 217, 313, 397, 476
NlaIII CATG 1 cut(s) 79
NlaIV GGNNCC 2 cut(s) 154, 484
PfeI GAWTC 1 cut(s) 559
PkrI GCNGC 5 cut(s) 112, 115, 143, 278, 281
PspFI CCCAGC 1 cut(s) 206
PspN4I GGNNCC 2 cut(s) 154, 484
PspPI GGNCC 2 cut(s) 170, 482
PstI CTGCAG 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 279
PsuI RGATCY 2 cut(s) 313, 476
PvuII CAGCTG 1 cut(s) 279
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 15, 83
SatI GCNGC 5 cut(s) 111, 114, 142, 277, 280
Sau3AI GATC 5 cut(s) 120, 217, 313, 397, 476
Sau96I GGNCC 2 cut(s) 170, 482
SduI GDGCHC 1 cut(s) 387
SetI ASST 8 cut(s) 49, 112, 118, 184, 202, 281, 381, 507
SfcI CTRYAG 1 cut(s) 111
Sse9I AATT 3 cut(s) 12, 212, 228
SsiI CCGC 1 cut(s) 156
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 4 cut(s) 288, 392, 460, 603
TaiI ACGT 1 cut(s) 507
TaqI TCGA 1 cut(s) 216
TasI AATT 3 cut(s) 12, 212, 228
TfiI GAWTC 1 cut(s) 559
Tru1I TTAA 2 cut(s) 15, 83
Tru9I TTAA 2 cut(s) 15, 83
TseI GCWGC 5 cut(s) 110, 113, 141, 276, 279
TspDTI ATGAA 1 cut(s) 177
XagI CCTNNNNNAGG 1 cut(s) 177
XapI RAATTY 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 2 cut(s) 73, 563
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.