Rw4G021820

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011771
Physical Location & Seq
Reverse (-)
46576870 .. 46577790
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G021820.1

Sequence Viewer

Length: 504 bp
ATGGCATTAACAAAAACCCAACTCTTAGTTTTAGCTGCAGCTTTGATCTACATCTCTCTTTCCTGCTGCAAAATGGGTTCCGCAGATGAAGATGGGCCTGAAACAGGTAATGTACCACAAACAGGTGGTGCTGGGGAATTCGATCCGGCTCAAATTGTTGCCAAAGCGGTGCTGTGTTTCAATAACAACTATGTATACAGCAGCTGCGAACAGTCTTTTAGATTGAATGAGAGTGGAGATCTGAATGTTCCAAAAGACAAAACCGACGAGTTCTGCAATGGTGCTTGCCTCACAGAGACACACCTTGTGCTCAACTGTGTTGATCACATTTTGGCCAACTTCGTCTTCTACAACAAGGCCACTATAAGAGATGTTAGAGATACTGTTCAGGCCGGATGTGGATCTGGCCCTGAAAGAGGCAACTTCAACGCACGCAAGCCTGCAACTTACCAGATTCTTGTTGGGCTTGTTTTGATGGTTTTGGGAAATGGATTACTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Endosulfine

Protein Analysis

167

Amino Acids

17.93

Weight (kDa)

4.91

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7731 PF24865 49 - 145 2.8e-43 Domain of unknown function (DUF7731)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011771)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49350 AT5G49350
fragaria_vesca FvH4_4g19330
malus_domestica MD13G1157600.v1.1 MD16G1158500.v1.1
prunus_persica Prupe.1G123300_v2.0.a1
pyrus_communis pycom13g13490 pycom16g13340
rosa_chinensis RchiOBHm_Chr4g0424351
rosa_laevigata RLG00000007482
rosa_multiflora Rmu_sc0005995.1_g000010
rosa_roxburghii Rroxscaffold_5G00366300
rosa_rugosa Rorug04G0196600 Rorug04G0196700
rosa_samantha Rh4AG253000 Rh4BG258400 Rh4CG269400 Rh4DG252800
rosa_wichuraiana Rw4G021820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 195
AciI CCGC 2 cut(s) 81, 167
AclWI GGATC 2 cut(s) 137, 409
AcoI YGGCCR 1 cut(s) 333
AcsI RAATTY 1 cut(s) 137
AdeI CACNNNGTG 1 cut(s) 307
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 104, 122, 416
AgsI TTSAA 3 cut(s) 181, 226, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 3 cut(s) 35, 41, 204
AluI AGCT 3 cut(s) 35, 41, 204
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 2 cut(s) 137, 409
AlwNI CAGNNNCTG 1 cut(s) 204
AoxI GGCC 5 cut(s) 95, 333, 357, 390, 406
ApeKI GCWGC 5 cut(s) 35, 38, 66, 201, 204
ApoI RAATTY 1 cut(s) 137
AspS9I GGNCC 2 cut(s) 95, 407
BalI TGGCCA 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 5 cut(s) 22, 50, 53, 191, 213
BccI CCATC 2 cut(s) 86, 469
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 36
BglII AGATCT 1 cut(s) 238
BisI GCNGC 5 cut(s) 36, 39, 67, 202, 205
BlsI GCNGC 5 cut(s) 37, 40, 68, 203, 206
BmgT120I GGNCC 2 cut(s) 95, 407
BmiI GGNNCC 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 337
BsaBI GATNNNNATC 2 cut(s) 50, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 122, 416
Bse3DI GCAATG 1 cut(s) 283
Bse8I GATNNNNATC 2 cut(s) 50, 400
BseGI GGATG 1 cut(s) 401
BseJI GATNNNNATC 2 cut(s) 50, 400
BseLI CCNNNNNNNGG 3 cut(s) 104, 122, 416
BseMI GCAATG 1 cut(s) 283
BseXI GCAGC 5 cut(s) 22, 50, 53, 191, 213
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 5 cut(s) 97, 335, 359, 392, 408
BsiHKAI GWGCWC 1 cut(s) 312
BsiSI CCGG 2 cut(s) 146, 393
BslI CCNNNNNNNGG 3 cut(s) 104, 122, 416
BsmAI GTCTC 1 cut(s) 290
BsnI GGCC 5 cut(s) 97, 335, 359, 392, 408
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 5 cut(s) 45, 142, 238, 322, 401
BspACI CCGC 2 cut(s) 81, 167
BspANI GGCC 5 cut(s) 97, 335, 359, 392, 408
BspLI GGNNCC 1 cut(s) 79
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 2 cut(s) 137, 409
BsrDI GCAATG 1 cut(s) 283
BssMI GATC 5 cut(s) 45, 142, 238, 322, 401
BssNAI GTATAC 1 cut(s) 196
Bst1107I GTATAC 1 cut(s) 196
Bst4CI ACNGT 4 cut(s) 213, 317, 385, 498
BstC8I GCNNGC 4 cut(s) 286, 433, 437, 441
BstDEI CTNAG 1 cut(s) 25
BstENI CCTNNNNNAGG 2 cut(s) 102, 414
BstF5I GGATG 1 cut(s) 401
BstKTI GATC 5 cut(s) 48, 145, 241, 325, 404
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 5 cut(s) 45, 142, 238, 322, 401
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 5 cut(s) 22, 50, 53, 191, 213
BstV2I GAAGAC 1 cut(s) 337
BstX2I RGATCY 2 cut(s) 238, 401
BstYI RGATCY 2 cut(s) 238, 401
BstZ17I GTATAC 1 cut(s) 196
BsuRI GGCC 5 cut(s) 97, 335, 359, 392, 408
BtsCI GGATG 1 cut(s) 401
Cac8I GCNNGC 4 cut(s) 286, 433, 437, 441
CaiI CAGNNNCTG 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 95, 407
Csp6I GTAC 1 cut(s) 113
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 1 cut(s) 25
DpnI GATC 5 cut(s) 47, 144, 240, 324, 403
DpnII GATC 5 cut(s) 45, 142, 238, 322, 401
DraIII CACNNNGTG 1 cut(s) 307
EaeI YGGCCR 1 cut(s) 333
EcoNI CCTNNNNNAGG 2 cut(s) 102, 414
EcoRI GAATTC 1 cut(s) 137
FaiI YATR 3 cut(s) 192, 196, 365
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 36, 39, 67, 202, 205
FokI GGATG 1 cut(s) 408
Fsp4HI GCNGC 5 cut(s) 36, 39, 67, 202, 205
GluI GCNGC 5 cut(s) 36, 39, 67, 202, 205
GsaI CCCAGC 1 cut(s) 135
HaeIII GGCC 5 cut(s) 97, 335, 359, 392, 408
HapII CCGG 2 cut(s) 146, 393
HinfI GANTC 1 cut(s) 454
HpaII CCGG 2 cut(s) 146, 393
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 243, 503
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 1 cut(s) 269
HpyCH4III ACNGT 4 cut(s) 213, 317, 385, 498
HpyCH4V TGCA 4 cut(s) 38, 69, 276, 443
HpyF3I CTNAG 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 5 cut(s) 45, 142, 238, 322, 401
Lsp1109I GCAGC 5 cut(s) 22, 50, 53, 191, 213
MalI GATC 5 cut(s) 47, 144, 240, 324, 403
MboI GATC 5 cut(s) 45, 142, 238, 322, 401
MboII GAAGA 2 cut(s) 101, 337
MflI RGATCY 2 cut(s) 238, 401
MhlI GDGCHC 1 cut(s) 312
MlsI TGGCCA 1 cut(s) 335
MluCI AATT 2 cut(s) 137, 153
MluNI TGGCCA 1 cut(s) 335
MnlI CCTC 2 cut(s) 299, 410
Mox20I TGGCCA 1 cut(s) 335
MscI TGGCCA 1 cut(s) 335
MseI TTAA 1 cut(s) 8
Msp20I TGGCCA 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 204
MspI CCGG 2 cut(s) 146, 393
NdeII GATC 5 cut(s) 45, 142, 238, 322, 401
NlaIV GGNNCC 1 cut(s) 79
PfeI GAWTC 1 cut(s) 454
PkrI GCNGC 5 cut(s) 37, 40, 68, 203, 206
PspFI CCCAGC 1 cut(s) 131
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 95, 407
PstI CTGCAG 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 204
PsuI RGATCY 2 cut(s) 238, 401
PvuII CAGCTG 1 cut(s) 204
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 5 cut(s) 36, 39, 67, 202, 205
Sau3AI GATC 5 cut(s) 45, 142, 238, 322, 401
Sau96I GGNCC 2 cut(s) 95, 407
SduI GDGCHC 1 cut(s) 312
SetI ASST 6 cut(s) 37, 43, 109, 127, 206, 306
SfcI CTRYAG 1 cut(s) 36
Sse9I AATT 2 cut(s) 137, 153
SsiI CCGC 2 cut(s) 81, 167
TaaI ACNGT 4 cut(s) 213, 317, 385, 498
TaqI TCGA 1 cut(s) 141
TasI AATT 2 cut(s) 137, 153
TfiI GAWTC 1 cut(s) 454
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TseI GCWGC 5 cut(s) 35, 38, 66, 201, 204
TspDTI ATGAA 1 cut(s) 102
XagI CCTNNNNNAGG 2 cut(s) 102, 414
XapI RAATTY 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmiI GTMKAC 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.