FvH4_4g21350

Major pollen allergen Ole e 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015912
Physical Location & Seq
Reverse (-)
24383452 .. 24385497
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21350.t1

Sequence Viewer

Length: 594 bp
ATGTGTTTTCATCACATCATCGTCACTATCAATAGTGTCACGAAAAAGACTAAAAAGCTAGATTGCCTAGTAGACATTACGAACCCTCAATCTCTATATAAGCAGCAGAAGAGATTAACAATGCAATTCATAATCCATATTATCTCCCTCTTTGTGTTCTTTGAATTGCTTTTGCATTTGGATTTTACAGCAATGTCTGCAATGCTGAAATTGTCATTTGTATTCTTTGTTTTGACCATTGCTCTTTTCGAATGTTTCACTACGAGCACTGAAGCAAGGGAAGTCAAAAATGAAAACGGGCGGGAGGCAAACGACAGAAAGTGGTGCATTGCTAAGCCATCTACTTCTGATGCTCAGCTTGAGATGAATGTCCAGTATGTCTGCCAACAAGGGTACTACAAGATTAGCTGCTCCGTTATCCAACCGGGAGGTTCCTGTTACATCCCTTCTAACAAGGTCTCATCTGCATCGATGGTCATGAACCTCTTTTTCCAACAAGATGGACAGACGGCCAATGCTTGCTATTTCAATGGCAGTGGACTGCTTGTTTATGAGAATCCTACTGTTGGAAGCTGCAACTACCCTCCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

198

Amino Acids

22.31

Weight (kDa)

8.05

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 107 - 180 2.1e-16 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66852 AT1G66855 AT1G66870 AT1G66870 AT4G16165
fragaria_vesca FvH4_4g21350
rosa_chinensis RchiOBHm_Chr4g0427121
rosa_laevigata RLG00000007260
rosa_multiflora Rmu_co7963664.1_g000001 Rmu_sc0000953.1_g000032
rosa_roxburghii Rroxscaffold_5G00368940
rosa_rugosa Rorug04G0216200
rosa_samantha Rh4AG271900 Rh4DG275400 Rh5CG462500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 72
AciI CCGC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 510
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 1 cut(s) 566
AgsI TTSAA 2 cut(s) 164, 529
AluBI AGCT 4 cut(s) 58, 358, 408, 573
AluI AGCT 4 cut(s) 58, 358, 408, 573
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 1 cut(s) 463
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 3 cut(s) 103, 408, 573
AsuC2I CCSGG 1 cut(s) 426
AsuII TTCGAA 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 3 cut(s) 115, 395, 560
BccI CCATC 3 cut(s) 346, 466, 494
BceAI ACGGC 1 cut(s) 525
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 2 cut(s) 59, 68
BisI GCNGC 3 cut(s) 104, 409, 574
BlpI GCTNAGC 2 cut(s) 333, 354
BlsI GCNGC 3 cut(s) 105, 410, 575
Bme1390I CCNGG 1 cut(s) 426
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 340, 476
Bpu1102I GCTNAGC 2 cut(s) 333, 354
Bpu14I TTCGAA 1 cut(s) 249
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 426
Bsa29I ATCGAT 1 cut(s) 470
BsaI GGTCTC 1 cut(s) 463
Bsc4I CCNNNNNNNGG 1 cut(s) 566
Bse1I ACTGG 1 cut(s) 373
Bse3DI GCAATG 4 cut(s) 198, 207, 237, 327
BseCI ATCGAT 1 cut(s) 470
BseGI GGATG 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 566
BseMI GCAATG 4 cut(s) 198, 207, 237, 327
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 373
BseXI GCAGC 3 cut(s) 115, 395, 560
BshFI GGCC 1 cut(s) 512
BshVI ATCGAT 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 269
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 1 cut(s) 566
BsmAI GTCTC 1 cut(s) 463
BsnI GGCC 1 cut(s) 512
Bso31I GGTCTC 1 cut(s) 463
Bsp119I TTCGAA 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 269
Bsp1720I GCTNAGC 2 cut(s) 333, 354
BspACI CCGC 1 cut(s) 301
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 1 cut(s) 367
BspDI ATCGAT 1 cut(s) 470
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 433
BspT104I TTCGAA 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 463
BsrDI GCAATG 4 cut(s) 198, 207, 237, 327
BsrI ACTGG 1 cut(s) 373
Bst4CI ACNGT 1 cut(s) 565
Bst6I CTCTTC 1 cut(s) 104
BstAPI GCANNNNNTGC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 520
BstDEI CTNAG 2 cut(s) 333, 354
BstF5I GGATG 1 cut(s) 441
BstMAI GTCTC 1 cut(s) 463
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 424
BstV1I GCAGC 3 cut(s) 115, 395, 560
BstXI CCANNNNNNTGG 1 cut(s) 500
Bsu15I ATCGAT 1 cut(s) 470
BsuRI GGCC 1 cut(s) 512
BsuTUI ATCGAT 1 cut(s) 470
BtsCI GGATG 1 cut(s) 441
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 2 cut(s) 267, 541
Cac8I GCNNGC 1 cut(s) 520
CciI TCATGA 1 cut(s) 477
ClaI ATCGAT 1 cut(s) 470
Csp6I GTAC 1 cut(s) 394
CspCI CAANNNNNGTGG 2 cut(s) 517, 552
CviAII CATG 1 cut(s) 478
CviJI RGCY 6 cut(s) 58, 337, 358, 408, 512, 573
CviKI_1 RGCY 6 cut(s) 58, 337, 358, 408, 512, 573
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 2 cut(s) 333, 354
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 291
FaeI CATG 1 cut(s) 481
FaiI YATR 7 cut(s) 97, 99, 131, 138, 378, 479, 552
FatI CATG 1 cut(s) 477
FauI CCCGC 1 cut(s) 294
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 104, 409, 574
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 3 cut(s) 104, 409, 574
FspBI CTAG 2 cut(s) 59, 68
GluI GCNGC 3 cut(s) 104, 409, 574
HaeIII GGCC 1 cut(s) 512
HapII CCGG 1 cut(s) 425
Hin1II CATG 1 cut(s) 481
HinfI GANTC 1 cut(s) 556
HpaII CCGG 1 cut(s) 425
Hpy166II GTNNAC 2 cut(s) 73, 539
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 2 cut(s) 40, 478
Hpy8I GTNNAC 2 cut(s) 73, 539
HpyAV CCTTC 1 cut(s) 456
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4V TGCA 6 cut(s) 124, 175, 200, 327, 467, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 2 cut(s) 333, 354
Hsp92II CATG 1 cut(s) 481
LmnI GCTCC 1 cut(s) 416
LpnPI CCDG 3 cut(s) 386, 438, 448
Lsp1109I GCAGC 3 cut(s) 115, 395, 560
LweI GCATC 2 cut(s) 340, 476
MaeI CTAG 2 cut(s) 59, 68
MaeIII GTNAC 3 cut(s) 22, 37, 437
MboII GAAGA 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 3 cut(s) 125, 164, 209
MmeI TCCRAC 3 cut(s) 445, 517, 547
MnlI CCTC 6 cut(s) 96, 158, 298, 422, 494, 594
MseI TTAA 1 cut(s) 116
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 426
MwoI GCNNNNNNNGC 1 cut(s) 197
NciI CCSGG 1 cut(s) 426
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 1 cut(s) 433
NmuCI GTSAC 2 cut(s) 22, 37
NspV TTCGAA 1 cut(s) 249
PagI TCATGA 1 cut(s) 477
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 3 cut(s) 105, 410, 575
PspN4I GGNNCC 1 cut(s) 433
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 3 cut(s) 104, 409, 574
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 269
SetI ASST 7 cut(s) 60, 360, 410, 433, 459, 486, 575
SfaNI GCATC 2 cut(s) 340, 476
SfuI TTCGAA 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 3 cut(s) 125, 164, 209
SsiI CCGC 1 cut(s) 301
SspMI CTAG 2 cut(s) 59, 68
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 1 cut(s) 565
TaqI TCGA 2 cut(s) 249, 470
TasI AATT 3 cut(s) 125, 164, 209
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 2 cut(s) 274, 541
TseFI GTSAC 2 cut(s) 22, 37
TseI GCWGC 3 cut(s) 103, 408, 573
Tsp45I GTSAC 2 cut(s) 22, 37
TspDTI ATGAA 4 cut(s) 118, 306, 380, 494
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 2 cut(s) 274, 541
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 2 cut(s) 59, 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.