Rh5CG462500

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0015912
Physical Location & Seq
Reverse (-)
64491241 .. 64491739
499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG462500.1

Sequence Viewer

Length: 417 bp
ATGGGTCCCACGCCCGATGTAGTTGCGCAACCACCGTCGTCGACGGGGAAGAGGTGGTGCGTGCCGAAACCGAACGCTACCCAGGCGACGCTGCAGAAGAACATAGACTACGTTTGCAGCATCGGAGTGGACTGCAAGCCCATACAGAACGGTGGGCCGTGCTTTGAGCCGAACACTGTGAAGTCACACGCGGCTTACGTCATGAACGCCTATTACCAGGATCATGGCCCACAGGATATTAACTGCGATTTCAACGGCACTGGAGTGGTCACCACCGCTGATCCCAGTTACAAAACGTGCACGTACCCTAACAAGGCGGAGAAGGAGGCAGTGGCGGGAAGCTCAATGAGAATGAGATGTAGCAAAGTGGCTCTGTTCTTTCTAATGGTCGGTTGTGGTGTAATGTTTAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

138

Amino Acids

14.89

Weight (kDa)

8.1

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 19 - 88 4e-24 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66852 AT1G66855 AT1G66870 AT1G66870 AT4G16165
fragaria_vesca FvH4_4g21350
rosa_chinensis RchiOBHm_Chr4g0427121
rosa_laevigata RLG00000007260
rosa_multiflora Rmu_co7963664.1_g000001 Rmu_sc0000953.1_g000032
rosa_roxburghii Rroxscaffold_5G00368940
rosa_rugosa Rorug04G0216200
rosa_samantha Rh4AG271900 Rh4DG275400 Rh5CG462500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 27
AccI GTMKAC 1 cut(s) 41
AccII CGCG 1 cut(s) 191
AciI CCGC 4 cut(s) 191, 276, 317, 335
AclWI GGATC 2 cut(s) 228, 275
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 313
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 2 cut(s) 81, 216
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 1 cut(s) 342
AluI AGCT 1 cut(s) 342
Alw21I GWGCWC 1 cut(s) 302
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 2 cut(s) 228, 275
AoxI GGCC 2 cut(s) 155, 226
ApaLI GTGCAC 1 cut(s) 298
ApeKI GCWGC 2 cut(s) 91, 117
AspLEI GCGC 1 cut(s) 28
AspS9I GGNCC 3 cut(s) 5, 155, 227
AsuHPI GGTGA 1 cut(s) 262
AvaII GGWCC 1 cut(s) 5
BaeGI GKGCMC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 78, 129
BceAI ACGGC 2 cut(s) 142, 271
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 83, 218
BfmI CTRYAG 1 cut(s) 92
BisI GCNGC 3 cut(s) 92, 118, 192
BlsI GCNGC 3 cut(s) 93, 119, 193
Bme1390I CCNGG 2 cut(s) 83, 218
Bme18I GGWCC 1 cut(s) 5
BmgT120I GGNCC 3 cut(s) 5, 155, 227
BmiI GGNNCC 2 cut(s) 6, 7
BmrFI CCNGG 2 cut(s) 83, 218
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 2 cut(s) 265, 285
BseBI CCWGG 2 cut(s) 83, 218
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 313
BseNI ACTGG 2 cut(s) 265, 285
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 2 cut(s) 78, 129
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 2 cut(s) 157, 228
BsiHKAI GWGCWC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 313
BsnI GGCC 2 cut(s) 157, 228
Bsp1286I GDGCHC 1 cut(s) 302
Bsp143I GATC 2 cut(s) 220, 280
BspACI CCGC 4 cut(s) 191, 276, 317, 335
BspANI GGCC 2 cut(s) 157, 228
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 6, 7
BspMAI CTGCAG 1 cut(s) 96
BspPI GGATC 2 cut(s) 228, 275
BsrI ACTGG 2 cut(s) 265, 285
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 2 cut(s) 220, 280
Bst2UI CCWGG 2 cut(s) 83, 218
Bst4CI ACNGT 3 cut(s) 36, 152, 178
Bst6I CTCTTC 1 cut(s) 44
BstBAI YACGTR 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 62, 137
BstEII GGTNACC 1 cut(s) 268
BstFNI CGCG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 28
BstKTI GATC 2 cut(s) 223, 283
BstMBI GATC 2 cut(s) 220, 280
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 2 cut(s) 83, 218
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 81, 216
BstSFI CTRYAG 1 cut(s) 92
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 2 cut(s) 78, 129
BstXI CCANNNNNNTGG 1 cut(s) 224
BsuRI GGCC 2 cut(s) 157, 228
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 3 cut(s) 174, 258, 336
Cac8I GCNNGC 2 cut(s) 62, 137
CciI TCATGA 1 cut(s) 201
CfoI GCGC 1 cut(s) 28
Cfr13I GGNCC 3 cut(s) 5, 155, 227
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 304
CviAII CATG 2 cut(s) 202, 224
CviJI RGCY 7 cut(s) 139, 157, 169, 194, 228, 342, 371
CviKI_1 RGCY 7 cut(s) 139, 157, 169, 194, 228, 342, 371
CviQI GTAC 1 cut(s) 304
DpnI GATC 2 cut(s) 222, 282
DpnII GATC 2 cut(s) 220, 280
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 5
Eco91I GGTNACC 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 5
EcoO65I GGTNACC 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 81, 216
FaeI CATG 2 cut(s) 205, 227
FaiI YATR 4 cut(s) 104, 143, 203, 225
FatI CATG 2 cut(s) 201, 223
FauI CCCGC 1 cut(s) 328
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 3 cut(s) 92, 118, 192
Fsp4HI GCNGC 3 cut(s) 92, 118, 192
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 1 cut(s) 27
GluI GCNGC 3 cut(s) 92, 118, 192
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 157, 228
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 28
Hin1II CATG 2 cut(s) 205, 227
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 3 cut(s) 42, 130, 300
Hpy188I TCNGA 1 cut(s) 125
Hpy188III TCNNGA 1 cut(s) 202
Hpy8I GTNNAC 3 cut(s) 42, 130, 300
Hpy99I CGWCG 4 cut(s) 40, 43, 46, 91
HpyAV CCTTC 1 cut(s) 316
HpyCH4III ACNGT 3 cut(s) 36, 152, 178
HpyCH4IV ACGT 4 cut(s) 111, 198, 296, 302
HpyCH4V TGCA 4 cut(s) 94, 117, 135, 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpySE526I ACGT 4 cut(s) 111, 198, 296, 302
Hsp92II CATG 2 cut(s) 205, 227
HspAI GCGC 1 cut(s) 26
KflI GGGWCCC 1 cut(s) 5
Kzo9I GATC 2 cut(s) 220, 280
LpnPI CCDG 7 cut(s) 68, 95, 203, 218, 230, 246, 298
Lsp1109I GCAGC 2 cut(s) 78, 129
LweI GCATC 1 cut(s) 129
MaeII ACGT 4 cut(s) 111, 198, 296, 302
MaeIII GTNAC 3 cut(s) 183, 268, 287
MalI GATC 2 cut(s) 222, 282
MboI GATC 2 cut(s) 220, 280
MboII GAAGA 2 cut(s) 61, 109
MhlI GDGCHC 1 cut(s) 302
MnlI CCTC 2 cut(s) 45, 319
MseI TTAA 2 cut(s) 240, 408
MslI CAYNNNNRTG 2 cut(s) 125, 263
MspA1I CMGCKG 1 cut(s) 278
MspR9I CCNGG 2 cut(s) 83, 218
MvaI CCWGG 2 cut(s) 83, 218
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 2 cut(s) 220, 280
NlaIII CATG 2 cut(s) 205, 227
NlaIV GGNNCC 2 cut(s) 6, 7
NmuCI GTSAC 2 cut(s) 183, 268
NsbI TGCGCA 1 cut(s) 27
OliI CACNNNNGTG 1 cut(s) 263
PagI TCATGA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PkrI GCNGC 3 cut(s) 93, 119, 193
Ppu21I YACGTR 1 cut(s) 303
PpuMI RGGWCCY 1 cut(s) 5
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 2 cut(s) 81, 216
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 2 cut(s) 81, 216
PspN4I GGNNCC 2 cut(s) 6, 7
PspPI GGNCC 3 cut(s) 5, 155, 227
PspPPI RGGWCCY 1 cut(s) 5
PsrI GAACNNNNNNTAC 4 cut(s) 92, 124, 197, 229
PstI CTGCAG 1 cut(s) 96
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 2 cut(s) 125, 263
SalI GTCGAC 1 cut(s) 40
SaqAI TTAA 2 cut(s) 240, 408
SatI GCNGC 3 cut(s) 92, 118, 192
Sau3AI GATC 2 cut(s) 220, 280
Sau96I GGNCC 3 cut(s) 5, 155, 227
ScrFI CCNGG 2 cut(s) 83, 218
SduI GDGCHC 1 cut(s) 302
SetI ASST 6 cut(s) 56, 114, 201, 299, 305, 344
SfaNI GCATC 1 cut(s) 129
SfcI CTRYAG 1 cut(s) 92
SgrDI CGTCGACG 1 cut(s) 40
SinI GGWCC 1 cut(s) 5
SmiMI CAYNNNNRTG 2 cut(s) 125, 263
SsiI CCGC 4 cut(s) 191, 276, 317, 335
SspI AATATT 1 cut(s) 412
StyD4I CCNGG 2 cut(s) 81, 216
TaaI ACNGT 3 cut(s) 36, 152, 178
TaiI ACGT 4 cut(s) 114, 201, 299, 305
TaqI TCGA 1 cut(s) 41
TauI GCSGC 1 cut(s) 194
Tru1I TTAA 2 cut(s) 240, 408
Tru9I TTAA 2 cut(s) 240, 408
TscAI CASTG 3 cut(s) 181, 265, 336
TseFI GTSAC 2 cut(s) 183, 268
TseI GCWGC 2 cut(s) 91, 117
Tsp45I GTSAC 2 cut(s) 183, 268
TspDTI ATGAA 1 cut(s) 218
TspRI CASTG 3 cut(s) 181, 265, 336
VneI GTGCAC 1 cut(s) 298
VpaK11BI GGWCC 1 cut(s) 5
XmiI GTMKAC 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.