FvH4_4g23540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009139
Physical Location & Seq
Forward (+)
26005621 .. 26009392
3772 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23540.t3

Sequence Viewer

Length: 537 bp
ATGATGAGAAGGCAGCAGCAGCATCATCATCAAGATCAACAATCGAGGGTGTTCTACGAGCTTTCCACTCTCGTCCTCAGCCTCCTCCGATCCCCGCCCACGCCGATTCAGTTTTCCGATCAGTCTGCGGCGGTTGCGTCCATGAATTCTGGTTCACAGCAGCCGATGATGACGATGACCGGCTCTTCGTCTAGGAGGGTGGCGGCTCCGGCGGCGCAGATCTCGCCGGCGGGGTTCGCGTCGCTGATGCTTGGGATTTCGATGGCGCTGATGCTGTGTGGATCGGTGACGTTCTTTATTGGGTTCTTGCTGATGCCGTGGGTGTTGGGGTTGGTTATGGTGTTTTATGTGGCCGGAGTTGTGTCTACTCTATCTGTTCTGGGGAAGTCGATTCTTTGTTACGCCTCGCCACCGCCGCCGACGTCCCCGCGGAAGGAGATACCCGATAAACCCACATTCTTCGACAGCTGCTACTATTATGCCAAACTTCCAACCTTCATCCACCAAATAATAATGTCTCTTCCATGCGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.56

Weight (kDa)

9.02

Isoelectric Point (pI)

72.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 365
AccII CGCG 2 cut(s) 239, 430
AclWI GGATC 2 cut(s) 84, 289
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 1 cut(s) 145
AcyI GRCGYC 1 cut(s) 422
AfiI CCNNNNNNNGG 1 cut(s) 433
AluBI AGCT 2 cut(s) 61, 468
AluI AGCT 2 cut(s) 61, 468
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 2 cut(s) 84, 289
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 5 cut(s) 13, 16, 19, 160, 468
ApoI RAATTY 1 cut(s) 145
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AspLEI GCGC 2 cut(s) 217, 268
AsuHPI GGTGA 1 cut(s) 298
BbvCI CCTCAGC 1 cut(s) 77
BbvI GCAGC 5 cut(s) 25, 28, 31, 172, 455
BccI CCATC 1 cut(s) 256
BceAI ACGGC 1 cut(s) 301
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 269
BglII AGATCT 1 cut(s) 219
BisI GCNGC 9 cut(s) 14, 17, 20, 129, 161, 204, 213, 416, 469
BlsI GCNGC 9 cut(s) 15, 18, 21, 130, 162, 205, 214, 417, 470
BmiI GGNNCC 1 cut(s) 207
BmsI GCATC 4 cut(s) 31, 237, 261, 303
Bpu10I CCTNAGC 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 317, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse118I RCCGGY 2 cut(s) 179, 226
BseDI CCNNGG 2 cut(s) 317, 428
BseGI GGATG 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 5 cut(s) 25, 28, 31, 172, 455
Bsh1236I CGCG 2 cut(s) 239, 430
BshFI GGCC 1 cut(s) 353
BsiSI CCGG 4 cut(s) 180, 209, 227, 354
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 1 cut(s) 353
Bsp143I GATC 5 cut(s) 34, 89, 118, 219, 281
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 90
BspFNI CGCG 2 cut(s) 239, 430
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 2 cut(s) 84, 289
BspQI GCTCTTC 1 cut(s) 190
BsrFI RCCGGY 2 cut(s) 179, 226
BssAI RCCGGY 2 cut(s) 179, 226
BssECI CCNNGG 2 cut(s) 317, 428
BssMI GATC 5 cut(s) 34, 89, 118, 219, 281
BssNI GRCGYC 1 cut(s) 422
Bst6I CTCTTC 2 cut(s) 190, 525
BstACI GRCGYC 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 1 cut(s) 77
BstDSI CCRYGG 2 cut(s) 317, 428
BstF5I GGATG 1 cut(s) 498
BstFNI CGCG 2 cut(s) 239, 430
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 2 cut(s) 217, 268
BstKTI GATC 5 cut(s) 37, 92, 121, 222, 284
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 5 cut(s) 34, 89, 118, 219, 281
BstMWI GCNNNNNNNGC 7 cut(s) 19, 134, 209, 212, 223, 236, 415
BstUI CGCG 2 cut(s) 239, 430
BstV1I GCAGC 5 cut(s) 25, 28, 31, 172, 455
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BsuRI GGCC 1 cut(s) 353
BtgI CCRYGG 2 cut(s) 317, 428
BtsCI GGATG 1 cut(s) 498
Cac8I GCNNGC 1 cut(s) 228
CfoI GCGC 2 cut(s) 217, 268
Cfr10I RCCGGY 2 cut(s) 179, 226
Cfr42I CCGCGG 1 cut(s) 431
CseI GACGC 2 cut(s) 126, 228
CviAII CATG 2 cut(s) 142, 525
CviJI RGCY 7 cut(s) 61, 81, 163, 183, 206, 353, 468
CviKI_1 RGCY 7 cut(s) 61, 81, 163, 183, 206, 353, 468
DdeI CTNAG 1 cut(s) 77
DpnI GATC 5 cut(s) 36, 91, 120, 221, 283
DpnII GATC 5 cut(s) 34, 89, 118, 219, 281
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 2 cut(s) 190, 525
EarI CTCTTC 2 cut(s) 190, 525
EcoRI GAATTC 1 cut(s) 145
FaeI CATG 2 cut(s) 145, 528
FaiI YATR 5 cut(s) 143, 338, 348, 480, 526
FaqI GGGAC 1 cut(s) 409
FatI CATG 2 cut(s) 141, 524
FauI CCCGC 3 cut(s) 102, 223, 435
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 9 cut(s) 14, 17, 20, 129, 161, 204, 213, 416, 469
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 9 cut(s) 14, 17, 20, 129, 161, 204, 213, 416, 469
FspBI CTAG 1 cut(s) 192
GlaI GCGC 2 cut(s) 216, 267
GluI GCNGC 9 cut(s) 14, 17, 20, 129, 161, 204, 213, 416, 469
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 353
HapII CCGG 4 cut(s) 180, 209, 227, 354
HgaI GACGC 2 cut(s) 126, 228
HhaI GCGC 2 cut(s) 217, 268
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 2 cut(s) 145, 528
Hin6I GCGC 2 cut(s) 215, 266
HinP1I GCGC 2 cut(s) 215, 266
HinfI GANTC 2 cut(s) 106, 391
HpaII CCGG 4 cut(s) 180, 209, 227, 354
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 2 cut(s) 155, 366
Hpy188I TCNGA 3 cut(s) 89, 118, 536
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 2 cut(s) 155, 366
Hpy99I CGWCG 2 cut(s) 244, 424
HpyAV CCTTC 3 cut(s) 3, 427, 505
HpyCH4IV ACGT 2 cut(s) 290, 422
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 134, 209, 212, 223, 236, 415
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 2 cut(s) 290, 422
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 2 cut(s) 145, 528
HspAI GCGC 2 cut(s) 215, 266
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
KspI CCGCGG 1 cut(s) 431
Kzo9I GATC 5 cut(s) 34, 89, 118, 219, 281
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 6 cut(s) 135, 193, 222, 240, 365, 367
Lsp1109I GCAGC 5 cut(s) 25, 28, 31, 172, 455
LweI GCATC 4 cut(s) 31, 237, 261, 303
MaeI CTAG 1 cut(s) 192
MaeII ACGT 2 cut(s) 290, 422
MaeIII GTNAC 2 cut(s) 286, 398
MalI GATC 5 cut(s) 36, 91, 120, 221, 283
MboI GATC 5 cut(s) 34, 89, 118, 219, 281
MboII GAAGA 3 cut(s) 177, 451, 512
MflI RGATCY 1 cut(s) 219
MluCI AATT 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 515
MnlI CCTC 6 cut(s) 39, 86, 92, 95, 189, 415
MreI CGCCGGCG 1 cut(s) 226
MroNI GCCGGC 1 cut(s) 226
MspA1I CMGCKG 2 cut(s) 430, 468
MspI CCGG 4 cut(s) 180, 209, 227, 354
MvnI CGCG 2 cut(s) 239, 430
MwoI GCNNNNNNNGC 7 cut(s) 19, 134, 209, 212, 223, 236, 415
NaeI GCCGGC 1 cut(s) 228
NdeII GATC 5 cut(s) 34, 89, 118, 219, 281
NgoMIV GCCGGC 1 cut(s) 226
NlaIII CATG 2 cut(s) 145, 528
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 190
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 106, 391
PkrI GCNGC 9 cut(s) 15, 18, 21, 130, 162, 205, 214, 417, 470
PspN4I GGNNCC 1 cut(s) 207
PsuI RGATCY 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 468
SacII CCGCGG 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 190
SatI GCNGC 9 cut(s) 14, 17, 20, 129, 161, 204, 213, 416, 469
Sau3AI GATC 5 cut(s) 34, 89, 118, 219, 281
SetI ASST 5 cut(s) 63, 293, 425, 470, 497
SfaNI GCATC 4 cut(s) 31, 237, 261, 303
Sfr303I CCGCGG 1 cut(s) 431
SgrAI CRCCGGYG 1 cut(s) 226
SgrBI CCGCGG 1 cut(s) 431
Sse9I AATT 1 cut(s) 145
SspMI CTAG 1 cut(s) 192
TaiI ACGT 2 cut(s) 293, 425
TaqI TCGA 4 cut(s) 44, 260, 389, 462
TasI AATT 1 cut(s) 145
TauI GCSGC 4 cut(s) 131, 206, 215, 418
TfiI GAWTC 2 cut(s) 106, 391
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 5 cut(s) 13, 16, 19, 160, 468
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 2 cut(s) 158, 487
XapI RAATTY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 365
XspI CTAG 1 cut(s) 192
ZraI GACGTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.