Rroxscaffold_5G00371770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009139
Physical Location & Seq
Forward (+)
53322265 .. 53323281
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371770.1

Sequence Viewer

Length: 516 bp
ATGATGAGAAGGGAGCAGCATCAGGACCAGCAATCTAGGGTTTTCTACGAGCTTTCCGCTCTAGTTCTCAGCCTTCTGCGGTCGCCGCCCACGCCGATTCAATTTTCCGATCAGTCTCCGGCGATTCCGCCGCCGCCGACGAATTCTTCTTCTTCTTCTTCTTCATCCAGGCGGCCGGCGGCGGCGGCGGCGGCTCAGATCTCGCCGGCGGGGTTCGCGTCGCTGATGCTGGGGATTTCGATGGCGCTGATGCTGTGTGGATCGGTGACGTTCTTCATCGGGTTCTTGTTGATGCCTTGGGTGCTCGGGTTGGTTATGGTGTTTTATGTGGCTGGAGTCGTCTCCACTCTCTCTGTTTTGGGGAAGTCGATTCTTTGTTACGCCTCGCCCCCGCCGACGTCACCGCGGAAGGAGATTCCGGATAAATTCACATTCTTCGACAGCCGCTACTATTATGCCAAACTTCCAACCTTCATCCACCAAATAATATGTCTCTTCCATGCAAAATCTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.74

Weight (kDa)

9.33

Isoelectric Point (pI)

77.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 401
AccBSI CCGCTC 1 cut(s) 59
AccII CGCG 2 cut(s) 218, 406
AccIII TCCGGA 1 cut(s) 418
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 2 cut(s) 142, 425
AcyI GRCGYC 1 cut(s) 398
AgsI TTSAA 1 cut(s) 101
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 1 cut(s) 52
AluI AGCT 1 cut(s) 52
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 120, 346, 497
AlwI GGATC 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 305
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 1 cut(s) 173
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 2 cut(s) 142, 425
ArsI GACNNNNNNTTYG 2 cut(s) 475, 507
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 277, 393
AvaI CYCGRG 1 cut(s) 305
AvaII GGWCC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 169
BcoDI GTCTC 3 cut(s) 120, 346, 497
BfaI CTAG 2 cut(s) 36, 62
BfoI RGCGCY 1 cut(s) 248
BglII AGATCT 1 cut(s) 198
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 169
BmsI GCATC 4 cut(s) 28, 216, 240, 282
BpmI CTGGAG 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 2 cut(s) 296, 404
BsaWI WCCGGW 1 cut(s) 418
Bse118I RCCGGY 2 cut(s) 175, 205
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 2 cut(s) 296, 404
BseGI GGATG 2 cut(s) 164, 474
BseMII CTCAG 2 cut(s) 82, 209
BseX3I CGGCCG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 229
Bsh1236I CGCG 2 cut(s) 218, 406
Bsh1285I CGRYCG 2 cut(s) 83, 176
BshFI GGCC 1 cut(s) 175
BsiEI CGRYCG 2 cut(s) 83, 176
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 305
BsiSI CCGG 4 cut(s) 119, 176, 206, 419
BsmAI GTCTC 3 cut(s) 120, 346, 497
BsmBI CGTCTC 1 cut(s) 346
BsnI GGCC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 306
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 3 cut(s) 109, 198, 260
BspANI GGCC 1 cut(s) 175
BspCNI CTCAG 2 cut(s) 81, 208
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 2 cut(s) 218, 406
BspPI GGATC 1 cut(s) 268
BsrBI CCGCTC 1 cut(s) 59
BsrFI RCCGGY 2 cut(s) 175, 205
BssAI RCCGGY 2 cut(s) 175, 205
BssECI CCNNGG 2 cut(s) 296, 404
BssMI GATC 3 cut(s) 109, 198, 260
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 169
Bst6I CTCTTC 1 cut(s) 500
BstACI GRCGYC 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 177, 207
BstDEI CTNAG 2 cut(s) 68, 195
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 2 cut(s) 164, 474
BstFNI CGCG 2 cut(s) 218, 406
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 3 cut(s) 112, 201, 263
BstMAI GTCTC 3 cut(s) 120, 346, 497
BstMBI GATC 3 cut(s) 109, 198, 260
BstMCI CGRYCG 2 cut(s) 83, 176
BstMWI GCNNNNNNNGC 7 cut(s) 85, 91, 185, 188, 191, 215, 301
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 167
BstUI CGCG 2 cut(s) 218, 406
BstV1I GCAGC 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BstZI CGGCCG 1 cut(s) 173
BsuRI GGCC 1 cut(s) 175
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 2 cut(s) 164, 474
Cac8I GCNNGC 2 cut(s) 177, 207
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 2 cut(s) 175, 205
Cfr13I GGNCC 1 cut(s) 25
Cfr42I CCGCGG 1 cut(s) 407
CseI GACGC 1 cut(s) 207
CviAII CATG 1 cut(s) 500
CviJI RGCY 6 cut(s) 52, 72, 175, 194, 332, 444
CviKI_1 RGCY 6 cut(s) 52, 72, 175, 194, 332, 444
DdeI CTNAG 2 cut(s) 68, 195
DpnI GATC 3 cut(s) 111, 200, 262
DpnII GATC 3 cut(s) 109, 198, 260
EaeI YGGCCR 1 cut(s) 173
EagI CGGCCG 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 500
EarI CTCTTC 1 cut(s) 500
EciI GGCGGA 1 cut(s) 117
EclXI CGGCCG 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 25
Eco52I CGGCCG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 167
EcoT14I CCWWGG 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 296
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 1 cut(s) 503
FaiI YATR 5 cut(s) 317, 327, 456, 490, 501
FatI CATG 1 cut(s) 499
FauI CCCGC 2 cut(s) 202, 399
FokI GGATG 2 cut(s) 151, 461
FspBI CTAG 2 cut(s) 36, 62
GlaI GCGC 1 cut(s) 246
GsaI CCCAGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 354
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 1 cut(s) 175
HapII CCGG 4 cut(s) 119, 176, 206, 419
HgaI GACGC 1 cut(s) 207
HhaI GCGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 1 cut(s) 503
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HinfI GANTC 5 cut(s) 97, 124, 336, 370, 415
HpaII CCGG 4 cut(s) 119, 176, 206, 419
HphI GGTGA 2 cut(s) 277, 393
Hpy188I TCNGA 3 cut(s) 109, 198, 511
Hpy188III TCNNGA 2 cut(s) 23, 419
Hpy99I CGWCG 3 cut(s) 142, 223, 400
HpyAV CCTTC 4 cut(s) 3, 83, 403, 481
HpyCH4IV ACGT 2 cut(s) 269, 398
HpyCH4V TGCA 1 cut(s) 503
HpyF10VI GCNNNNNNNGC 7 cut(s) 85, 91, 185, 188, 191, 215, 301
HpyF3I CTNAG 2 cut(s) 68, 195
HpySE526I ACGT 2 cut(s) 269, 398
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 1 cut(s) 503
HspAI GCGC 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 418
KroI GCCGGC 2 cut(s) 175, 205
KroNI GCCGGC 2 cut(s) 177, 207
KspI CCGCGG 1 cut(s) 407
Kzo9I GATC 3 cut(s) 109, 198, 260
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 4 cut(s) 28, 216, 240, 282
MaeI CTAG 2 cut(s) 36, 62
MaeII ACGT 2 cut(s) 269, 398
MaeIII GTNAC 3 cut(s) 265, 377, 399
MalI GATC 3 cut(s) 111, 200, 262
MbiI CCGCTC 1 cut(s) 59
MboI GATC 3 cut(s) 109, 198, 260
MboII GAAGA 9 cut(s) 138, 141, 144, 147, 150, 153, 265, 427, 487
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 3 cut(s) 101, 142, 425
MlyI GAGTC 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 491
MnlI CCTC 1 cut(s) 394
MreI CGCCGGCG 1 cut(s) 205
MroI TCCGGA 1 cut(s) 418
MroNI GCCGGC 2 cut(s) 175, 205
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 4 cut(s) 119, 176, 206, 419
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 2 cut(s) 218, 406
MwoI GCNNNNNNNGC 7 cut(s) 85, 91, 185, 188, 191, 215, 301
NaeI GCCGGC 2 cut(s) 177, 207
NdeII GATC 3 cut(s) 109, 198, 260
NgoMIV GCCGGC 2 cut(s) 175, 205
NlaIII CATG 1 cut(s) 503
NmuCI GTSAC 2 cut(s) 265, 399
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PdiI GCCGGC 2 cut(s) 177, 207
PfeI GAWTC 4 cut(s) 97, 124, 370, 415
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
Psp6I CCWGG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 229
PspGI CCWGG 1 cut(s) 167
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 1 cut(s) 198
SacII CCGCGG 1 cut(s) 407
Sau3AI GATC 3 cut(s) 109, 198, 260
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 1 cut(s) 345
ScrFI CCNGG 1 cut(s) 169
SduI GDGCHC 1 cut(s) 306
SetI ASST 4 cut(s) 54, 272, 401, 473
SfaNI GCATC 4 cut(s) 28, 216, 240, 282
Sfr303I CCGCGG 1 cut(s) 407
SgrAI CRCCGGYG 1 cut(s) 205
SgrBI CCGCGG 1 cut(s) 407
SinI GGWCC 1 cut(s) 25
Sse9I AATT 3 cut(s) 101, 142, 425
SspMI CTAG 2 cut(s) 36, 62
StyD4I CCNGG 1 cut(s) 167
StyI CCWWGG 1 cut(s) 296
TaiI ACGT 2 cut(s) 272, 401
TaqI TCGA 3 cut(s) 239, 368, 438
TasI AATT 3 cut(s) 101, 142, 425
TfiI GAWTC 4 cut(s) 97, 124, 370, 415
TseFI GTSAC 2 cut(s) 265, 399
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 265, 399
TspDTI ATGAA 3 cut(s) 153, 265, 463
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 2 cut(s) 142, 425
XspI CTAG 2 cut(s) 36, 62
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.