Rw4G025630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009139
Physical Location & Seq
Forward (+)
51794770 .. 51796442
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025630.1

Sequence Viewer

Length: 441 bp
ATGATGAGAAGGGAGCAGCATCAGGACCAGCAATCTAGGGTTTTCTACGAGCTTTCCGCTCTAGTTCTCAGCCTTCTGCGGTCGCCGCCCACGCCGATTCAATTTTCCGATCAGTCTCCGGCCATTCCGCCGCCGACGAATTCTTCTTCTTCTTCTGTGTCTAGCTCTTCATCCAGGCGGCCGGCGGCGGCGCAGATCTCGCCGGCGGGGTTCGCGTCGCTGATGCTGGGGATTTCGATGGCGCTGATGCTGTGTGGATCGGTGACGTTCTTCATCGGGTTCTTGTTGATGCCTTGGGTGCTCGGGTTGGTTATGGTGTTTTATGTGGCTGGAGTCGTCTCCACTCTCTCTGTTTTGGGGAAGTCGATTCTTTGTTACGCCTCGCCGCCGCCGCCGCCGACGTCACCGCGGAAGGAGATTCCGGCATGGAAGTTATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.71

Weight (kDa)

9.78

Isoelectric Point (pI)

82.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 404
AccBSI CCGCTC 1 cut(s) 59
AccII CGCG 2 cut(s) 215, 409
AclWI GGATC 1 cut(s) 265
AcoI YGGCCR 2 cut(s) 120, 179
AcsI RAATTY 1 cut(s) 139
AcyI GRCGYC 1 cut(s) 401
AgsI TTSAA 1 cut(s) 101
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 2 cut(s) 52, 165
AluI AGCT 2 cut(s) 52, 165
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 120, 343
AlwI GGATC 1 cut(s) 265
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 2 cut(s) 120, 179
ApeKI GCWGC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 2 cut(s) 193, 244
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 274, 396
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 232
BciT130I CCWGG 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 120, 343
BfaI CTAG 3 cut(s) 36, 62, 162
BfoI RGCGCY 1 cut(s) 245
BglII AGATCT 1 cut(s) 195
Bme1390I CCNGG 1 cut(s) 175
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 175
BmsI GCATC 4 cut(s) 28, 213, 237, 279
BpmI CTGGAG 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 293, 407
Bse118I RCCGGY 2 cut(s) 181, 202
BseBI CCWGG 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 293, 407
BseGI GGATG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 82
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 226
Bsh1236I CGCG 2 cut(s) 215, 409
Bsh1285I CGRYCG 2 cut(s) 83, 182
BshFI GGCC 2 cut(s) 122, 181
BsiEI CGRYCG 2 cut(s) 83, 182
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 4 cut(s) 119, 182, 203, 422
BsmAI GTCTC 2 cut(s) 120, 343
BsmBI CGTCTC 1 cut(s) 343
BsnI GGCC 2 cut(s) 122, 181
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 3 cut(s) 109, 195, 257
BspANI GGCC 2 cut(s) 122, 181
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 2 cut(s) 215, 409
BspPI GGATC 1 cut(s) 265
BspQI GCTCTTC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 59
BsrFI RCCGGY 2 cut(s) 181, 202
BssAI RCCGGY 2 cut(s) 181, 202
BssECI CCNNGG 2 cut(s) 293, 407
BssMI GATC 3 cut(s) 109, 195, 257
BssNI GRCGYC 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 172
BstACI GRCGYC 1 cut(s) 401
BstC8I GCNNGC 2 cut(s) 183, 204
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 1 cut(s) 170
BstFNI CGCG 2 cut(s) 215, 409
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 2 cut(s) 193, 244
BstKTI GATC 3 cut(s) 112, 198, 260
BstMAI GTCTC 2 cut(s) 120, 343
BstMBI GATC 3 cut(s) 109, 195, 257
BstMCI CGRYCG 2 cut(s) 83, 182
BstMWI GCNNNNNNNGC 7 cut(s) 85, 91, 199, 212, 298, 391, 394
BstNI CCWGG 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 173
BstUI CGCG 2 cut(s) 215, 409
BstV1I GCAGC 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 2 cut(s) 122, 181
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 1 cut(s) 170
Cac8I GCNNGC 2 cut(s) 183, 204
CfoI GCGC 2 cut(s) 193, 244
Cfr10I RCCGGY 2 cut(s) 181, 202
Cfr13I GGNCC 1 cut(s) 25
Cfr42I CCGCGG 1 cut(s) 410
CseI GACGC 1 cut(s) 204
CviAII CATG 1 cut(s) 426
CviJI RGCY 6 cut(s) 52, 72, 122, 165, 181, 329
CviKI_1 RGCY 6 cut(s) 52, 72, 122, 165, 181, 329
DdeI CTNAG 1 cut(s) 68
DpnI GATC 3 cut(s) 111, 197, 259
DpnII GATC 3 cut(s) 109, 195, 257
EaeI YGGCCR 2 cut(s) 120, 179
EagI CGGCCG 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
EciI GGCGGA 1 cut(s) 117
EclXI CGGCCG 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 25
Eco52I CGGCCG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 293
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 1 cut(s) 429
FaiI YATR 3 cut(s) 314, 324, 427
FatI CATG 1 cut(s) 425
FauI CCCGC 1 cut(s) 199
FokI GGATG 1 cut(s) 157
FspBI CTAG 3 cut(s) 36, 62, 162
GlaI GCGC 2 cut(s) 192, 243
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 2 cut(s) 122, 181
HapII CCGG 4 cut(s) 119, 182, 203, 422
HgaI GACGC 1 cut(s) 204
HhaI GCGC 2 cut(s) 193, 244
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 1 cut(s) 429
Hin6I GCGC 2 cut(s) 191, 242
HinP1I GCGC 2 cut(s) 191, 242
HinfI GANTC 4 cut(s) 97, 333, 367, 418
HpaII CCGG 4 cut(s) 119, 182, 203, 422
HphI GGTGA 2 cut(s) 274, 396
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 1 cut(s) 23
Hpy99I CGWCG 3 cut(s) 139, 220, 403
HpyAV CCTTC 3 cut(s) 3, 83, 406
HpyCH4IV ACGT 2 cut(s) 266, 401
HpyF10VI GCNNNNNNNGC 7 cut(s) 85, 91, 199, 212, 298, 391, 394
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 2 cut(s) 266, 401
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 1 cut(s) 429
HspAI GCGC 2 cut(s) 191, 242
KroI GCCGGC 2 cut(s) 181, 202
KroNI GCCGGC 2 cut(s) 183, 204
KspI CCGCGG 1 cut(s) 410
Kzo9I GATC 3 cut(s) 109, 195, 257
LguI GCTCTTC 1 cut(s) 172
LmnI GCTCC 1 cut(s) 13
Lsp1109I GCAGC 1 cut(s) 28
LweI GCATC 4 cut(s) 28, 213, 237, 279
MaeI CTAG 3 cut(s) 36, 62, 162
MaeII ACGT 2 cut(s) 266, 401
MaeIII GTNAC 3 cut(s) 262, 374, 402
MalI GATC 3 cut(s) 111, 197, 259
MbiI CCGCTC 1 cut(s) 59
MboI GATC 3 cut(s) 109, 195, 257
MboII GAAGA 6 cut(s) 135, 138, 141, 144, 159, 262
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 2 cut(s) 101, 139
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 1 cut(s) 391
MreI CGCCGGCG 1 cut(s) 202
MroNI GCCGGC 2 cut(s) 181, 202
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 4 cut(s) 119, 182, 203, 422
MspR9I CCNGG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 175
MvnI CGCG 2 cut(s) 215, 409
MwoI GCNNNNNNNGC 7 cut(s) 85, 91, 199, 212, 298, 391, 394
NaeI GCCGGC 2 cut(s) 183, 204
NdeII GATC 3 cut(s) 109, 195, 257
NgoMIV GCCGGC 2 cut(s) 181, 202
NlaIII CATG 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 262, 402
PciSI GCTCTTC 1 cut(s) 172
PdiI GCCGGC 2 cut(s) 183, 204
PfeI GAWTC 3 cut(s) 97, 367, 418
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 173
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 1 cut(s) 195
SacII CCGCGG 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 172
Sau3AI GATC 3 cut(s) 109, 195, 257
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 1 cut(s) 342
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 1 cut(s) 303
SetI ASST 4 cut(s) 54, 167, 269, 404
SfaNI GCATC 4 cut(s) 28, 213, 237, 279
Sfr303I CCGCGG 1 cut(s) 410
SgrAI CRCCGGYG 1 cut(s) 202
SgrBI CCGCGG 1 cut(s) 410
SinI GGWCC 1 cut(s) 25
Sse9I AATT 2 cut(s) 101, 139
SspMI CTAG 3 cut(s) 36, 62, 162
StyD4I CCNGG 1 cut(s) 173
StyI CCWWGG 1 cut(s) 293
TaiI ACGT 2 cut(s) 269, 404
TaqI TCGA 2 cut(s) 236, 365
TasI AATT 2 cut(s) 101, 139
TauI GCSGC 9 cut(s) 88, 133, 181, 188, 191, 388, 391, 394, 397
TfiI GAWTC 3 cut(s) 97, 367, 418
TseFI GTSAC 2 cut(s) 262, 402
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 262, 402
TspDTI ATGAA 2 cut(s) 159, 262
VpaK11BI GGWCC 1 cut(s) 25
XapI RAATTY 1 cut(s) 139
XspI CTAG 3 cut(s) 36, 62, 162
ZraI GACGTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.