FvH4_4g25160

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002110
Physical Location & Seq
Reverse (-)
27023726 .. 27027959
4234 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25160.t3

Sequence Viewer

Length: 1860 bp
ATGGCTGAGGAGCAAATTAATTTTGAAGAGGAGGAATATGGAGCTGCTCAGAAGCTCCAATATCAGGGAAGTGGAGCCATATCTGCTCTTGCAGATGAAGAACTGATGGTGGAAGATGATGAGTATGACGATCTCTATGATGATGTTAATGTTGGAGAGGGTTTCCTCCAGATGCATCGATCTGAGGCACCAGCAGGGGCTGGTAGTGTTGGGAATGGAGGACCTCAAGCTCAGAAAACTGTTGCTCCTGAACTAAGAGTTCAAGCTGGTGCGTCTCAGGAAATGAAAATTCCGGGGGTTTCAGTTGGAGGAAATTATTCTACTGTTCCTGAACAGAAGGTTCAGCCACCAGTGGCTAATGTGCCTGAAACTCAAGTCAGGCATATGGGGTTTCAGGGTTCAACAACTATACCCTCGAATGTTGGTGTTGATTCTTTGGAAGTTACTGGAAAATTTGCTAATGAGCCTCTTCAATCAATGAATTCTGGCACTACTGGTCCTTCAGCTGTTGCACAGGTGCCTGCTAATCAAATGAATATGAAGGTCAATCTTAATCGTCCAATGGTTAATGATAACCAAATCCGTCCTCCAGTTGAGAATGGTTCAGCCACACTGTTTGTGGGAGACTTACATTGGTGGACAACTGATGCTGAACTGGAAAGTGTTTTATCTCAGTTTGGGAGGGTGAAAGAAATTAAGTTCTTTGATGAAAGAGCCAGTGGTAAATCCAAAGGTTATTGTCAGGTAGATTTTTATGATCCAGCAGCAGCCAGTGCATGCAAAGAGGGAATGGATGGTTATGTTTTCAATGGGAGAGCTTGTGTTGTGGCCTTTGCTTCTTCACAAACTTTAAAGCAGATGGGAGCTAATTATGTGAACAAGTCCCAAGGGCAGGTTCAGACTCAGCCCCAAGGGAGAAGGCCCATGAATGATGGTGCTGGGAGGGGGGGAGGCATGAATTTTCAAGGTGGAGACACTGGGAGAAATTTTGGGAGGGGCAACTGGGGACGAGGTGGACAGGGAGTACCCAACAGAGGTCCTGGTGGACCAGGCCGGGGAAGAGGAGGGGCCATGGGTGCAAGGAACATGGTTGGAAACAATGCTGGGGTTGGAACTGGTGGTAATGGAGGAGGCTATGGACAGGGTCTTGCAGGTCCTGGTTTTGGCGGTCCTGTGGGAGGTATGATGAATGCACCTGGCATGATGGGTCCTGGGTTCGATCCAACATATATGGGTCGAGGAGGTGGTTATGGGGGGTTCCCTGGTCCTGGTTTTCCTGGAATGCTTCCCCAGTTTCCTGGTATGGGACTTGCTGGGGTTGCTCCTCATGTAAACCCAGCTTTCTTTGGGAGGGGAATGGCAACAAGCGGCATGGGGATGATGGGTTCTTCTGGAATGGAGGGGCATCACGCTCCTATGTGGAATGATCCAAGTATGGCTGGGTGGGGAGGAGAAGAACAGGATCAAAGAACTAGGGAGTCGAGCTATGGGGGTGATGATGGTGGTTCTGAGTATGGGAACTATGTAGAGGCAAATCTTGAGAAATCAGCCAAGTCAAGTGCTGTCTCAAGGGACAGAGAACGGGGATCTGAGCGTGAGTGGACAGGATCATCTGAGAGGAGGCATCGTGATGAGAGGGAACAAGACTTTGACAGGTCTGAAAGGGAGCACAAGGAGCCCAGGTACAAAGAAGAAAAAGATAGTCACAGAGAGCATCGTCGAAGAGAGCGGGATGTGGGTTATGATGATGACTGGGACAGAGGACAATCTTCTTCAAGACCTCGCAGCAGGTCCAAAGCAGTGCCAGAAGATGATCATCGTTCTCGATCAAGGGATGTGGATTATGGGAAGAGGAGATGA

Protein Analysis

620

Amino Acids

66.28

Weight (kDa)

5.45

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 205 - 274 1.7e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 883, 1142, 1779
AccB1I GGYRCC 2 cut(s) 187, 517
AccBSI CCGCTC 1 cut(s) 1729
AciI CCGC 3 cut(s) 1167, 1368, 1729
AclWI GGATC 6 cut(s) 752, 1214, 1421, 1470, 1594, 1615
AcsI RAATTY 5 cut(s) 288, 452, 481, 958, 985
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 2 cut(s) 1026, 1685
AfiI CCNNNNNNNGG 8 cut(s) 64, 892, 1034, 1055, 1163, 1178, 1268, 1304
AgsI TTSAA 7 cut(s) 26, 263, 402, 473, 808, 965, 1776
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AloI GAACNNNNNNTCC 2 cut(s) 1369, 1401
AluBI AGCT 9 cut(s) 44, 55, 230, 266, 506, 818, 866, 1340, 1485
AluI AGCT 9 cut(s) 44, 55, 230, 266, 506, 818, 866, 1340, 1485
Alw21I GWGCWC 1 cut(s) 1671
Alw26I GTCTC 4 cut(s) 279, 618, 966, 1570
AlwI GGATC 6 cut(s) 752, 1214, 1421, 1470, 1594, 1615
AlwNI CAGNNNCTG 2 cut(s) 200, 1157
AoxI GGCC 4 cut(s) 828, 920, 1051, 1068
ApeKI GCWGC 4 cut(s) 44, 764, 767, 1785
ApoI RAATTY 5 cut(s) 288, 452, 481, 958, 985
AseI ATTAAT 1 cut(s) 18
Asp700I GAANNNNTTC 1 cut(s) 316
AsuC2I CCSGG 2 cut(s) 294, 1055
AsuHPI GGTGA 2 cut(s) 697, 1505
AvaII GGWCC 9 cut(s) 221, 497, 1037, 1046, 1154, 1169, 1208, 1265, 1791
BanI GGYRCC 2 cut(s) 187, 517
BanII GRGCYC 1 cut(s) 1680
Bbv12I GWGCWC 1 cut(s) 1671
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 4 cut(s) 31, 776, 779, 1797
BccI CCATC 7 cut(s) 100, 788, 853, 926, 1198, 1375, 1493
BclI TGATCA 1 cut(s) 1813
BcnI CCSGG 2 cut(s) 294, 1055
BcoDI GTCTC 4 cut(s) 279, 618, 966, 1570
BfaI CTAG 1 cut(s) 1473
BfuAI ACCTGC 3 cut(s) 883, 1142, 1779
BisI GCNGC 5 cut(s) 45, 765, 768, 1369, 1786
BlsI GCNGC 5 cut(s) 46, 766, 769, 1370, 1787
Bme18I GGWCC 9 cut(s) 221, 497, 1037, 1046, 1154, 1169, 1208, 1265, 1791
BmiI GGNNCC 7 cut(s) 76, 189, 519, 1069, 1209, 1259, 1677
BmrI ACTGGG 4 cut(s) 987, 1012, 1285, 1762
BmsI GCATC 6 cut(s) 162, 184, 637, 1414, 1633, 1723
BmuI ACTGGG 4 cut(s) 987, 1012, 1285, 1762
BpmI CTGGAG 2 cut(s) 152, 573
Bpu10I CCTNAGC 1 cut(s) 6
BpuEI CTTGAG 4 cut(s) 210, 357, 1552, 1559
BpuMI CCSGG 2 cut(s) 294, 1055
Bsa29I ATCGAT 1 cut(s) 178
BsaBI GATNNNNATC 1 cut(s) 1815
BsaJI CCNNGG 8 cut(s) 293, 886, 910, 1054, 1071, 1211, 1261, 1679
BsaXI ACNNNNNCTCC 8 cut(s) 229, 259, 805, 835, 1612, 1642, 1667, 1697
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 892, 1034, 1055, 1163, 1178, 1268, 1304
Bse8I GATNNNNATC 1 cut(s) 1815
BseCI ATCGAT 1 cut(s) 178
BseDI CCNNGG 8 cut(s) 293, 886, 910, 1054, 1071, 1211, 1261, 1679
BseGI GGATG 4 cut(s) 799, 1383, 1738, 1840
BseJI GATNNNNATC 1 cut(s) 1815
BseLI CCNNNNNNNGG 8 cut(s) 64, 892, 1034, 1055, 1163, 1178, 1268, 1304
BseMII CTCAG 9 cut(s) 62, 174, 245, 290, 686, 917, 1500, 1581, 1605
BseRI GAGGAG 8 cut(s) 23, 44, 1077, 1143, 1254, 1314, 1464, 1633
BseXI GCAGC 4 cut(s) 31, 776, 779, 1797
BseYI CCCAGC 5 cut(s) 938, 1103, 1313, 1336, 1439
BshFI GGCC 4 cut(s) 830, 922, 1053, 1070
BshNI GGYRCC 2 cut(s) 187, 517
BshVI ATCGAT 1 cut(s) 178
BsiHKAI GWGCWC 1 cut(s) 1671
BsiSI CCGG 2 cut(s) 293, 1054
BslFI GGGAC 5 cut(s) 868, 1020, 1320, 1586, 1769
BslI CCNNNNNNNGG 8 cut(s) 64, 892, 1034, 1055, 1163, 1178, 1268, 1304
BsmAI GTCTC 4 cut(s) 279, 618, 966, 1570
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 5 cut(s) 868, 1020, 1320, 1586, 1769
BsmI GAATGC 2 cut(s) 1195, 1287
BsnI GGCC 4 cut(s) 830, 922, 1053, 1070
Bsp1286I GDGCHC 2 cut(s) 1671, 1680
Bsp19I CCATGG 1 cut(s) 1071
BspACI CCGC 3 cut(s) 1167, 1368, 1729
BspANI GGCC 4 cut(s) 830, 922, 1053, 1070
BspCNI CTCAG 9 cut(s) 61, 175, 244, 289, 685, 916, 1501, 1582, 1606
BspDI ATCGAT 1 cut(s) 178
BspLI GGNNCC 7 cut(s) 76, 189, 519, 1069, 1209, 1259, 1677
BspMI ACCTGC 3 cut(s) 883, 1142, 1779
BspPI GGATC 6 cut(s) 752, 1214, 1421, 1470, 1594, 1615
BspT107I GGYRCC 2 cut(s) 187, 517
BsrBI CCGCTC 1 cut(s) 1729
BssECI CCNNGG 8 cut(s) 293, 886, 910, 1054, 1071, 1211, 1261, 1679
BssT1I CCWWGG 3 cut(s) 886, 910, 1071
Bst4CI ACNGT 3 cut(s) 241, 325, 615
Bst6I CTCTTC 5 cut(s) 21, 474, 1054, 1717, 1844
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 522, 778
BstDSI CCRYGG 1 cut(s) 1071
BstENI CCTNNNNNAGG 1 cut(s) 1176
BstF5I GGATG 4 cut(s) 799, 1383, 1738, 1840
BstMAI GTCTC 4 cut(s) 279, 618, 966, 1570
BstMWI GCNNNNNNNGC 5 cut(s) 83, 773, 1076, 1319, 1675
BstNSI RCATGY 1 cut(s) 780
BstV1I GCAGC 4 cut(s) 31, 776, 779, 1797
BstX2I RGATCY 1 cut(s) 1586
BstXI CCANNNNNNTGG 1 cut(s) 1298
BstYI RGATCY 1 cut(s) 1586
Bsu15I ATCGAT 1 cut(s) 178
BsuRI GGCC 4 cut(s) 830, 922, 1053, 1070
BsuTUI ATCGAT 1 cut(s) 178
BtgI CCRYGG 1 cut(s) 1071
BtsCI GGATG 4 cut(s) 799, 1383, 1738, 1840
BtsI GCAGTG 1 cut(s) 1806
BtsIMutI CAGTG 6 cut(s) 357, 611, 724, 778, 975, 1806
BveI ACCTGC 3 cut(s) 883, 1142, 1779
Cac8I GCNNGC 2 cut(s) 522, 778
CaiI CAGNNNCTG 2 cut(s) 200, 1157
ClaI ATCGAT 1 cut(s) 178
CseI GACGC 1 cut(s) 261
Csp6I GTAC 2 cut(s) 1025, 1684
CspCI CAANNNNNGTGG 4 cut(s) 598, 633, 1818, 1853
CviAII CATG 8 cut(s) 777, 925, 955, 1072, 1087, 1201, 1328, 1372
CviQI GTAC 2 cut(s) 1025, 1684
DraI TTTAAA 1 cut(s) 852
Eam1104I CTCTTC 5 cut(s) 21, 474, 1054, 1717, 1844
EarI CTCTTC 5 cut(s) 21, 474, 1054, 1717, 1844
Eco130I CCWWGG 3 cut(s) 886, 910, 1071
Eco24I GRGCYC 1 cut(s) 1680
Eco47I GGWCC 9 cut(s) 221, 497, 1037, 1046, 1154, 1169, 1208, 1265, 1791
Eco57I CTGAAG 1 cut(s) 486
EcoNI CCTNNNNNAGG 1 cut(s) 1176
EcoO109I RGGNCCY 4 cut(s) 221, 1037, 1154, 1208
EcoRI GAATTC 1 cut(s) 481
EcoT14I CCWWGG 3 cut(s) 886, 910, 1071
EcoT22I ATGCAT 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 1680
ErhI CCWWGG 3 cut(s) 886, 910, 1071
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 8 cut(s) 780, 928, 958, 1075, 1090, 1204, 1331, 1375
FaqI GGGAC 5 cut(s) 868, 1020, 1320, 1586, 1769
FatI CATG 8 cut(s) 776, 924, 954, 1071, 1086, 1200, 1327, 1371
FauI CCCGC 1 cut(s) 1722
FauNDI CATATG 1 cut(s) 384
FbaI TGATCA 1 cut(s) 1813
Fnu4HI GCNGC 5 cut(s) 45, 765, 768, 1369, 1786
FokI GGATG 4 cut(s) 806, 1390, 1745, 1847
FriOI GRGCYC 1 cut(s) 1680
Fsp4HI GCNGC 5 cut(s) 45, 765, 768, 1369, 1786
FspBI CTAG 1 cut(s) 1473
GluI GCNGC 5 cut(s) 45, 765, 768, 1369, 1786
GsaI CCCAGC 5 cut(s) 942, 1107, 1317, 1340, 1443
GsuI CTGGAG 2 cut(s) 152, 573
HaeIII GGCC 4 cut(s) 830, 922, 1053, 1070
HapII CCGG 2 cut(s) 293, 1054
HgaI GACGC 1 cut(s) 261
Hin1II CATG 8 cut(s) 780, 928, 958, 1075, 1090, 1204, 1331, 1375
HinfI GANTC 3 cut(s) 431, 901, 1478
HpaII CCGG 2 cut(s) 293, 1054
HphI GGTGA 2 cut(s) 697, 1505
Hpy166II GTNNAC 6 cut(s) 639, 877, 1016, 1046, 1333, 1602
Hpy188I TCNGA 8 cut(s) 51, 184, 234, 900, 1510, 1591, 1615, 1660
Hpy188III TCNNGA 9 cut(s) 169, 248, 278, 329, 1392, 1538, 1628, 1776, 1824
Hpy8I GTNNAC 6 cut(s) 639, 877, 1016, 1046, 1333, 1602
Hpy99I CGWCG 1 cut(s) 1722
HpyAV CCTTC 4 cut(s) 331, 510, 535, 912
HpyCH4III ACNGT 3 cut(s) 241, 325, 615
HpyCH4V TGCA 8 cut(s) 92, 175, 512, 776, 780, 1079, 1151, 1193
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 773, 1076, 1319, 1675
Hsp92II CATG 8 cut(s) 780, 928, 958, 1075, 1090, 1204, 1331, 1375
Ksp22I TGATCA 1 cut(s) 1813
Lsp1109I GCAGC 4 cut(s) 31, 776, 779, 1797
LweI GCATC 6 cut(s) 162, 184, 637, 1414, 1633, 1723
MaeI CTAG 1 cut(s) 1473
MaeIII GTNAC 2 cut(s) 442, 1703
MbiI CCGCTC 1 cut(s) 1729
MflI RGATCY 1 cut(s) 1586
MhlI GDGCHC 2 cut(s) 1671, 1680
MlyI GAGTC 2 cut(s) 895, 1487
MmeI TCCRAC 5 cut(s) 133, 286, 1072, 1090, 1247
Mph1103I ATGCAT 1 cut(s) 177
MroXI GAANNNNTTC 1 cut(s) 316
MseI TTAA 6 cut(s) 18, 147, 552, 567, 696, 851
MslI CAYNNNNRTG 2 cut(s) 1376, 1629
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 2 cut(s) 293, 1054
Mva1269I GAATGC 2 cut(s) 1195, 1287
MwoI GCNNNNNNNGC 5 cut(s) 83, 773, 1076, 1319, 1675
NciI CCSGG 2 cut(s) 294, 1055
NcoI CCATGG 1 cut(s) 1071
NdeI CATATG 1 cut(s) 384
NlaIII CATG 8 cut(s) 780, 928, 958, 1075, 1090, 1204, 1331, 1375
NlaIV GGNNCC 7 cut(s) 76, 189, 519, 1069, 1209, 1259, 1677
NmuCI GTSAC 1 cut(s) 1703
NsiI ATGCAT 1 cut(s) 177
NspI RCATGY 1 cut(s) 780
PaeI GCATGC 1 cut(s) 780
PctI GAATGC 2 cut(s) 1195, 1287
PdmI GAANNNNTTC 1 cut(s) 316
PfeI GAWTC 1 cut(s) 431
PflFI GACNNNGTC 1 cut(s) 1143
PfoI TCCNGGA 1 cut(s) 1276
PkrI GCNGC 5 cut(s) 46, 766, 769, 1370, 1787
PleI GAGTC 2 cut(s) 895, 1486
PpsI GAGTC 2 cut(s) 895, 1486
PpuMI RGGWCCY 4 cut(s) 221, 1037, 1154, 1208
PshBI ATTAAT 1 cut(s) 18
Psp5II RGGWCCY 4 cut(s) 221, 1037, 1154, 1208
PspFI CCCAGC 5 cut(s) 938, 1103, 1313, 1336, 1439
PspN4I GGNNCC 7 cut(s) 76, 189, 519, 1069, 1209, 1259, 1677
PspPPI RGGWCCY 4 cut(s) 221, 1037, 1154, 1208
PstNI CAGNNNCTG 2 cut(s) 200, 1157
PsuI RGATCY 1 cut(s) 1586
PsyI GACNNNGTC 1 cut(s) 1143
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 2 cut(s) 1026, 1685
RsaNI GTAC 2 cut(s) 1025, 1684
RseI CAYNNNNRTG 2 cut(s) 1376, 1629
SaqAI TTAA 6 cut(s) 18, 147, 552, 567, 696, 851
SatI GCNGC 5 cut(s) 45, 765, 768, 1369, 1786
SchI GAGTC 2 cut(s) 895, 1487
SduI GDGCHC 2 cut(s) 1671, 1680
SfaNI GCATC 6 cut(s) 162, 184, 637, 1414, 1633, 1723
SinI GGWCC 9 cut(s) 221, 497, 1037, 1046, 1154, 1169, 1208, 1265, 1791
SmiMI CAYNNNNRTG 2 cut(s) 1376, 1629
SmlI CTYRAG 4 cut(s) 225, 372, 1538, 1567
SmoI CTYRAG 4 cut(s) 225, 372, 1538, 1567
SphI GCATGC 1 cut(s) 780
SsiI CCGC 3 cut(s) 1167, 1368, 1729
SspMI CTAG 1 cut(s) 1473
StyI CCWWGG 3 cut(s) 886, 910, 1071
TaaI ACNGT 3 cut(s) 241, 325, 615
TaqI TCGA 7 cut(s) 178, 416, 1218, 1237, 1481, 1720, 1825
TauI GCSGC 1 cut(s) 1371
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 6 cut(s) 18, 147, 552, 567, 696, 851
Tru9I TTAA 6 cut(s) 18, 147, 552, 567, 696, 851
TscAI CASTG 6 cut(s) 357, 618, 724, 778, 982, 1806
TseFI GTSAC 1 cut(s) 1703
TseI GCWGC 4 cut(s) 44, 764, 767, 1785
Tsp45I GTSAC 1 cut(s) 1703
TspDTI ATGAA 9 cut(s) 111, 299, 494, 548, 554, 723, 941, 971, 1202
TspGWI ACGGA 1 cut(s) 572
TspRI CASTG 6 cut(s) 357, 618, 724, 778, 982, 1806
Tth111I GACNNNGTC 1 cut(s) 1143
VpaK11BI GGWCC 9 cut(s) 221, 497, 1037, 1046, 1154, 1169, 1208, 1265, 1791
VspI ATTAAT 1 cut(s) 18
XagI CCTNNNNNAGG 1 cut(s) 1176
XapI RAATTY 5 cut(s) 288, 452, 481, 958, 985
XceI RCATGY 1 cut(s) 780
XcmI CCANNNNNNNNNTGG 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 316
XspI CTAG 1 cut(s) 1473
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.