Rw4G027270

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002110
Physical Location & Seq
Reverse (-)
53694712 .. 53696656
1945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027270.1

Sequence Viewer

Length: 1854 bp
ATGGATCCAATGGCCGAGGAGCAAATAGATTATGAAGAGGAGGAGTATGGAGGTGCTCAGAAGCTCCAATATCAGGAAAGTGGAGCCATACCTGCTCTTGCTGATGAAGAACCGATGGTAGAAGATGACGAGTATGACGATCTCTACAATGATGTTAATGTTGGAGAAGGTTTCCTGCAGATGCACCGACCTGAGGCACCACACCCACCTGCTGGTGTTGGCAATGGAGGACTCCAAGCTCAGAAAAATAATGTTCCTGAAGTAAGAGTTCAAGCTGGTGTTTCTCAGGAACTGAAAAATCCGGGATTTTCAGTTGAAGGAAAGTATTCTAGTGTTCCTGAACAGAAGGATCAGCCCCCAGTGTCTAAGGTGCCAGAACTGGGATCTCAAAAGGGGAGGGTTATGGAGATGACTCATGATGCTCACGTCAGGAATATGGGTTTTCAGGGGACAGCAACTATGCCGTCCAATGTTGGTGTTGATTCTTCTTCAGATCTTACTGGGAAAATTGCCAATGGGCCTATTCCATCAATGAATTCTAGCACTACTGGTCCTCCAGCTGTTCCACAGATGCCGGCTAATCAAATGAATATGAAGATAAATGTTAATCGTCCAATGGTTAATGAAAACCAAATTCGTCCCCCAATTGAGAATGGTTCAGCCACACTGTTTGTGGGAGAACTCCATTGGTGGACTACTGATGCTGAACTGGAAAGTGTTTTGTCTCAGTTTGGGAGGATCAAGGAAATTAAGTTCTTTGATGAGAGAGCCAGTGGTAAATCCAAAGGTTATTGTCAGGTAGATTTTTATGATCCAGTGGCTGCCACTTGTGTTGTCGCCTTTGCTTCTTCACAAACTTTAAAGCAGATGGGAGATTCTTATGTCAGCAAATCCCAAGGCCAGGTTCAGACTCAGCCCCAAGGAAGAAGGCCTATGAATGACGGTGCTGGGAGAGGGGGAAATATGAATTATCAAGGTGGAGACACAGGGAGAAATTTTGGAAGGGGTAATAATTGGGGACGAGGTGGACAGGGAGTACTCAACAGAGGTCCTGGTGGTGGAGGACCAGGGAGGGGACGAGGAGGGGCCATGGGTGCAAGGAACATGGTTGGAAACAATGCTGGGGTTGGAACTGGTGCTAATGGAGGAGGCTATGGACAGGGCCTTGGAGGTCCTGGATTTGGCGGTCCTGTTGGAGGTATGATGAATGGACCGGGTATGATGGGTCCTGGGTTTGATCCAACGTATATGGGTCGAGGAGGTGGTTACGGAGGGTTTCCTGGTCCTGGTTTTCCTGGAATGCTTCCTCAATTTCCTGCTTTCTTTGGCAGGGGAATGGCTACGAATGGGATGGGGATGATGGGTTCTTCTGGAATGGAGGGGCATCATGCCCCGATGTGGAATGATCCAAGCATGGCTGGGTGGACTGGAGAAGAACAGGATCGAAGAACTAGGGAATCAAGCTATGGTGGTGATGATGGTGCATCTGAGTATGGAAACTATGGGGAGGCAACTCATGAGAAATCAGTTAGGTCAAGTGCTGCCCCTAGGGAAAGGGAACGGGAATCTGAGCGTGACTGGACGGGAACCTCTGAGAGGAGGCATCGTGATGAAAGGGAACAAGACTGGGATAGGTCTGAGAGGGAGCACAGGGAGCCCAGGTACAAGGAAGAAAAAGATAGTTACCGAGACCATAGGCGGAGGGAGCGTGACGTGGCCTATGAGGATGACCGGGACAGAGGACACTCTTCTTCAAGACCTCGCAGCAGGTCCAAGGCAATGCCAGAAGATGATCATCGTTCTCGATCAAGGGATATGGATTATGGGAAGAGGAGACGATTGCCATCTGAGTGA

Protein Analysis

617

Amino Acids

67.13

Weight (kDa)

5.51

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 223 - 279 3.4e-09 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 3 cut(s) 100, 217, 1758
AccB1I GGYRCC 2 cut(s) 196, 370
AccB7I CCANNNNNTGG 1 cut(s) 212
AciI CCGC 2 cut(s) 1185, 1699
AclWI GGATC 8 cut(s) 12, 357, 391, 746, 806, 1232, 1400, 1449
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 3 cut(s) 535, 633, 994
AcuI CTGAAG 2 cut(s) 279, 474
AfaI GTAC 2 cut(s) 1038, 1664
AgsI TTSAA 3 cut(s) 272, 317, 1755
AjiI CACGTC 2 cut(s) 427, 1714
AjnI CCWGG 9 cut(s) 900, 1051, 1066, 1174, 1228, 1279, 1285, 1294, 1658
AjuI GAANNNNNNNTTGG 2 cut(s) 888, 920
AloI GAACNNNNNNTCC 4 cut(s) 737, 769, 1348, 1380
AluBI AGCT 5 cut(s) 64, 239, 275, 560, 1464
AluI AGCT 5 cut(s) 64, 239, 275, 560, 1464
Alw21I GWGCWC 2 cut(s) 58, 1650
Alw26I GTCTC 4 cut(s) 729, 975, 1683, 1828
AlwI GGATC 8 cut(s) 12, 357, 391, 746, 806, 1232, 1400, 1449
AlwNI CAGNNNCTG 2 cut(s) 292, 821
AoxI GGCC 7 cut(s) 12, 518, 898, 929, 1086, 1162, 1716
ApeKI GCWGC 3 cut(s) 821, 1541, 1764
ApoI RAATTY 3 cut(s) 535, 633, 994
Asp700I GAANNNNTTC 1 cut(s) 325
AspA2I CCTAGG 1 cut(s) 1547
AsuC2I CCSGG 3 cut(s) 303, 1215, 1733
AsuHPI GGTGA 1 cut(s) 1484
AvaII GGWCC 9 cut(s) 551, 1049, 1064, 1172, 1187, 1211, 1226, 1283, 1770
AvrII CCTAGG 1 cut(s) 1547
AxyI CCTNAGG 1 cut(s) 192
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 196, 370
BanII GRGCYC 1 cut(s) 1659
Bbv12I GWGCWC 2 cut(s) 58, 1650
BbvI GCAGC 3 cut(s) 808, 1528, 1776
BccI CCATC 8 cut(s) 109, 535, 862, 1216, 1345, 1354, 1472, 1852
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 9 cut(s) 902, 1053, 1068, 1176, 1230, 1281, 1287, 1296, 1660
BclI TGATCA 1 cut(s) 1792
BcnI CCSGG 3 cut(s) 303, 1215, 1733
BcoDI GTCTC 4 cut(s) 729, 975, 1683, 1828
BfaI CTAG 4 cut(s) 330, 540, 1452, 1548
BfmI CTRYAG 1 cut(s) 176
BfuAI ACCTGC 3 cut(s) 100, 217, 1758
BglII AGATCT 1 cut(s) 493
BisI GCNGC 3 cut(s) 822, 1542, 1765
BlnI CCTAGG 1 cut(s) 1547
BlsI GCNGC 3 cut(s) 823, 1543, 1766
BmcAI AGTACT 1 cut(s) 1038
Bme18I GGWCC 9 cut(s) 551, 1049, 1064, 1172, 1187, 1211, 1226, 1283, 1770
BmgBI CACGTC 2 cut(s) 427, 1714
BmiI GGNNCC 8 cut(s) 6, 85, 198, 372, 1087, 1227, 1588, 1656
BmrI ACTGGG 4 cut(s) 353, 389, 510, 1636
BmsI GCATC 7 cut(s) 171, 409, 561, 691, 1393, 1493, 1612
BmuI ACTGGG 4 cut(s) 353, 389, 510, 1636
BpmI CTGGAG 2 cut(s) 540, 1449
BpuMI CCSGG 3 cut(s) 303, 1215, 1733
BsaBI GATNNNNATC 2 cut(s) 1794, 1843
BsaI GGTCTC 1 cut(s) 1683
BsaXI ACNNNNNCTCC 8 cut(s) 538, 568, 1591, 1621, 1646, 1676, 1693, 1723
Bse118I RCCGGY 1 cut(s) 574
Bse21I CCTNAGG 1 cut(s) 192
Bse3DI GCAATG 2 cut(s) 229, 1785
Bse8I GATNNNNATC 2 cut(s) 1794, 1843
BseBI CCWGG 9 cut(s) 902, 1053, 1068, 1176, 1230, 1281, 1287, 1296, 1660
BseGI GGATG 3 cut(s) 1356, 1362, 1732
BseJI GATNNNNATC 2 cut(s) 1794, 1843
BseMI GCAATG 2 cut(s) 229, 1785
BseRI GAGGAG 8 cut(s) 32, 53, 56, 1095, 1161, 1272, 1612, 1846
BseXI GCAGC 3 cut(s) 808, 1528, 1776
BseYI CCCAGC 3 cut(s) 947, 1121, 1418
BshFI GGCC 7 cut(s) 14, 520, 900, 931, 1088, 1164, 1718
BshNI GGYRCC 2 cut(s) 196, 370
BsiHKAI GWGCWC 2 cut(s) 58, 1650
BsiSI CCGG 4 cut(s) 302, 575, 1214, 1732
BslFI GGGAC 5 cut(s) 463, 624, 1032, 1089, 1748
BsmAI GTCTC 4 cut(s) 729, 975, 1683, 1828
BsmBI CGTCTC 1 cut(s) 1828
BsmFI GGGAC 5 cut(s) 463, 624, 1032, 1089, 1748
BsmI GAATGC 1 cut(s) 1305
BsnI GGCC 7 cut(s) 14, 520, 900, 931, 1088, 1164, 1718
Bso31I GGTCTC 1 cut(s) 1683
Bsp1286I GDGCHC 3 cut(s) 58, 1650, 1659
Bsp19I CCATGG 1 cut(s) 1089
BspACI CCGC 2 cut(s) 1185, 1699
BspANI GGCC 7 cut(s) 14, 520, 900, 931, 1088, 1164, 1718
BspHI TCATGA 2 cut(s) 415, 1516
BspLI GGNNCC 8 cut(s) 6, 85, 198, 372, 1087, 1227, 1588, 1656
BspMAI CTGCAG 1 cut(s) 180
BspMI ACCTGC 3 cut(s) 100, 217, 1758
BspPI GGATC 8 cut(s) 12, 357, 391, 746, 806, 1232, 1400, 1449
BspT107I GGYRCC 2 cut(s) 196, 370
BspTNI GGTCTC 1 cut(s) 1683
BsrDI GCAATG 2 cut(s) 229, 1785
BsrFI RCCGGY 1 cut(s) 574
BssAI RCCGGY 1 cut(s) 574
BssT1I CCWWGG 6 cut(s) 895, 919, 1089, 1165, 1547, 1773
Bst2UI CCWGG 9 cut(s) 902, 1053, 1068, 1176, 1230, 1281, 1287, 1296, 1660
Bst4CI ACNGT 2 cut(s) 669, 944
Bst6I CTCTTC 3 cut(s) 30, 1753, 1823
BstC8I GCNNGC 1 cut(s) 576
BstDSI CCRYGG 1 cut(s) 1089
BstENI CCTNNNNNAGG 2 cut(s) 1194, 1594
BstF5I GGATG 3 cut(s) 1356, 1362, 1732
BstMAI GTCTC 4 cut(s) 729, 975, 1683, 1828
BstMWI GCNNNNNNNGC 4 cut(s) 92, 1094, 1654, 1705
BstNI CCWGG 9 cut(s) 902, 1053, 1068, 1176, 1230, 1281, 1287, 1296, 1660
BstSFI CTRYAG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 808, 1528, 1776
BstX2I RGATCY 3 cut(s) 4, 383, 493
BstYI RGATCY 3 cut(s) 4, 383, 493
Bsu36I CCTNAGG 1 cut(s) 192
BsuRI GGCC 7 cut(s) 14, 520, 900, 931, 1088, 1164, 1718
BtgI CCRYGG 1 cut(s) 1089
BtrI CACGTC 2 cut(s) 427, 1714
BtsCI GGATG 3 cut(s) 1356, 1362, 1732
BtsIMutI CAGTG 4 cut(s) 366, 665, 778, 822
BveI ACCTGC 3 cut(s) 100, 217, 1758
Cac8I GCNNGC 1 cut(s) 576
CaiI CAGNNNCTG 2 cut(s) 292, 821
CciI TCATGA 2 cut(s) 415, 1516
Cfr10I RCCGGY 1 cut(s) 574
Csp6I GTAC 2 cut(s) 1037, 1663
CspCI CAANNNNNGTGG 4 cut(s) 652, 687, 814, 849
CviAII CATG 6 cut(s) 416, 1090, 1105, 1388, 1414, 1517
CviQI GTAC 2 cut(s) 1037, 1663
DraI TTTAAA 1 cut(s) 861
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 3 cut(s) 30, 1753, 1823
EarI CTCTTC 3 cut(s) 30, 1753, 1823
EciI GGCGGA 1 cut(s) 1714
Eco130I CCWWGG 6 cut(s) 895, 919, 1089, 1165, 1547, 1773
Eco147I AGGCCT 1 cut(s) 931
Eco24I GRGCYC 1 cut(s) 1659
Eco31I GGTCTC 1 cut(s) 1683
Eco47I GGWCC 9 cut(s) 551, 1049, 1064, 1172, 1187, 1211, 1226, 1283, 1770
Eco57I CTGAAG 2 cut(s) 279, 474
Eco81I CCTNAGG 1 cut(s) 192
EcoNI CCTNNNNNAGG 2 cut(s) 1194, 1594
EcoO109I RGGNCCY 4 cut(s) 1049, 1162, 1172, 1226
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 9 cut(s) 900, 1051, 1066, 1174, 1228, 1279, 1285, 1294, 1658
EcoT14I CCWWGG 6 cut(s) 895, 919, 1089, 1165, 1547, 1773
EcoT38I GRGCYC 1 cut(s) 1659
ErhI CCWWGG 6 cut(s) 895, 919, 1089, 1165, 1547, 1773
Esp3I CGTCTC 1 cut(s) 1828
FaeI CATG 6 cut(s) 419, 1093, 1108, 1391, 1417, 1520
FaqI GGGAC 5 cut(s) 463, 624, 1032, 1089, 1748
FatI CATG 6 cut(s) 415, 1089, 1104, 1387, 1413, 1516
FbaI TGATCA 1 cut(s) 1792
Fnu4HI GCNGC 3 cut(s) 822, 1542, 1765
FokI GGATG 3 cut(s) 1363, 1369, 1739
FriOI GRGCYC 1 cut(s) 1659
Fsp4HI GCNGC 3 cut(s) 822, 1542, 1765
FspBI CTAG 4 cut(s) 330, 540, 1452, 1548
GluI GCNGC 3 cut(s) 822, 1542, 1765
GsaI CCCAGC 3 cut(s) 951, 1125, 1422
GsuI CTGGAG 2 cut(s) 540, 1449
HaeIII GGCC 7 cut(s) 14, 520, 900, 931, 1088, 1164, 1718
HapII CCGG 4 cut(s) 302, 575, 1214, 1732
Hin1II CATG 6 cut(s) 419, 1093, 1108, 1391, 1417, 1520
HinfI GANTC 7 cut(s) 231, 412, 482, 875, 910, 1457, 1565
HpaII CCGG 4 cut(s) 302, 575, 1214, 1732
HphI GGTGA 1 cut(s) 1484
Hpy166II GTNNAC 3 cut(s) 693, 1028, 1425
Hpy188I TCNGA 9 cut(s) 60, 243, 493, 909, 1489, 1570, 1594, 1639, 1849
Hpy8I GTNNAC 3 cut(s) 693, 1028, 1425
HpyAV CCTTC 5 cut(s) 161, 311, 340, 921, 996
HpyCH4III ACNGT 2 cut(s) 669, 944
HpyCH4IV ACGT 3 cut(s) 426, 1244, 1713
HpyCH4V TGCA 4 cut(s) 178, 184, 1097, 1484
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 1094, 1654, 1705
HpySE526I ACGT 3 cut(s) 426, 1244, 1713
Hsp92II CATG 6 cut(s) 419, 1093, 1108, 1391, 1417, 1520
KroI GCCGGC 1 cut(s) 574
KroNI GCCGGC 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 1792
LmnI GCTCC 6 cut(s) 19, 69, 83, 1645, 1654, 1705
Lsp1109I GCAGC 3 cut(s) 808, 1528, 1776
LweI GCATC 7 cut(s) 171, 409, 561, 691, 1393, 1493, 1612
MaeI CTAG 4 cut(s) 330, 540, 1452, 1548
MaeII ACGT 3 cut(s) 426, 1244, 1713
MaeIII GTNAC 4 cut(s) 1265, 1574, 1682, 1709
MfeI CAATTG 1 cut(s) 645
MflI RGATCY 3 cut(s) 4, 383, 493
MhlI GDGCHC 3 cut(s) 58, 1650, 1659
MluCI AATT 9 cut(s) 507, 535, 633, 645, 747, 967, 994, 1012, 1310
MlyI GAGTC 3 cut(s) 225, 406, 904
MmeI TCCRAC 5 cut(s) 142, 1090, 1108, 1174, 1265
MroNI GCCGGC 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 325
MseI TTAA 5 cut(s) 156, 606, 621, 750, 860
MslI CAYNNNNRTG 2 cut(s) 1608, 1849
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 4 cut(s) 302, 575, 1214, 1732
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 1 cut(s) 1305
MvaI CCWGG 9 cut(s) 902, 1053, 1068, 1176, 1230, 1281, 1287, 1296, 1660
MwoI GCNNNNNNNGC 4 cut(s) 92, 1094, 1654, 1705
NaeI GCCGGC 1 cut(s) 576
NciI CCSGG 3 cut(s) 303, 1215, 1733
NcoI CCATGG 1 cut(s) 1089
NgoMIV GCCGGC 1 cut(s) 574
NlaIII CATG 6 cut(s) 419, 1093, 1108, 1391, 1417, 1520
NlaIV GGNNCC 8 cut(s) 6, 85, 198, 372, 1087, 1227, 1588, 1656
NmeAIII GCCGAG 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 1574, 1709
PagI TCATGA 2 cut(s) 415, 1516
PaqCI CACCTGC 1 cut(s) 217
PceI AGGCCT 1 cut(s) 931
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 1305
PdiI GCCGGC 1 cut(s) 576
PdmI GAANNNNTTC 1 cut(s) 325
PfeI GAWTC 4 cut(s) 482, 875, 1457, 1565
PflMI CCANNNNNTGG 1 cut(s) 212
PfoI TCCNGGA 3 cut(s) 301, 1174, 1294
PkrI GCNGC 3 cut(s) 823, 1543, 1766
PleI GAGTC 3 cut(s) 225, 406, 904
PpsI GAGTC 3 cut(s) 225, 406, 904
PpuMI RGGWCCY 3 cut(s) 1049, 1172, 1226
Psp5II RGGWCCY 3 cut(s) 1049, 1172, 1226
Psp6I CCWGG 9 cut(s) 900, 1051, 1066, 1174, 1228, 1279, 1285, 1294, 1658
PspFI CCCAGC 3 cut(s) 947, 1121, 1418
PspGI CCWGG 9 cut(s) 900, 1051, 1066, 1174, 1228, 1279, 1285, 1294, 1658
PspN4I GGNNCC 8 cut(s) 6, 85, 198, 372, 1087, 1227, 1588, 1656
PspPPI RGGWCCY 3 cut(s) 1049, 1172, 1226
PstI CTGCAG 1 cut(s) 180
PstNI CAGNNNCTG 2 cut(s) 292, 821
PsuI RGATCY 3 cut(s) 4, 383, 493
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 2 cut(s) 1038, 1664
RsaNI GTAC 2 cut(s) 1037, 1663
RseI CAYNNNNRTG 2 cut(s) 1608, 1849
SaqAI TTAA 5 cut(s) 156, 606, 621, 750, 860
SatI GCNGC 3 cut(s) 822, 1542, 1765
ScaI AGTACT 1 cut(s) 1038
SchI GAGTC 3 cut(s) 225, 406, 904
SduI GDGCHC 3 cut(s) 58, 1650, 1659
SfaNI GCATC 7 cut(s) 171, 409, 561, 691, 1393, 1493, 1612
SfcI CTRYAG 1 cut(s) 176
SinI GGWCC 9 cut(s) 551, 1049, 1064, 1172, 1187, 1211, 1226, 1283, 1770
SmiMI CAYNNNNRTG 2 cut(s) 1608, 1849
Sse9I AATT 9 cut(s) 507, 535, 633, 645, 747, 967, 994, 1012, 1310
SseBI AGGCCT 1 cut(s) 931
SsiI CCGC 2 cut(s) 1185, 1699
SspMI CTAG 4 cut(s) 330, 540, 1452, 1548
StuI AGGCCT 1 cut(s) 931
StyI CCWWGG 6 cut(s) 895, 919, 1089, 1165, 1547, 1773
TaaI ACNGT 2 cut(s) 669, 944
TaiI ACGT 3 cut(s) 429, 1247, 1716
TaqI TCGA 3 cut(s) 1255, 1444, 1804
TasI AATT 9 cut(s) 507, 535, 633, 645, 747, 967, 994, 1012, 1310
TatI WGTACW 1 cut(s) 1036
TfiI GAWTC 4 cut(s) 482, 875, 1457, 1565
Tru1I TTAA 5 cut(s) 156, 606, 621, 750, 860
Tru9I TTAA 5 cut(s) 156, 606, 621, 750, 860
TscAI CASTG 4 cut(s) 366, 672, 778, 822
TseFI GTSAC 2 cut(s) 1574, 1709
TseI GCWGC 3 cut(s) 821, 1541, 1764
Tsp45I GTSAC 2 cut(s) 1574, 1709
TspGWI ACGGA 1 cut(s) 1284
TspRI CASTG 4 cut(s) 366, 672, 778, 822
Van91I CCANNNNNTGG 1 cut(s) 212
VpaK11BI GGWCC 9 cut(s) 551, 1049, 1064, 1172, 1187, 1211, 1226, 1283, 1770
XagI CCTNNNNNAGG 2 cut(s) 1194, 1594
XapI RAATTY 3 cut(s) 535, 633, 994
XcmI CCANNNNNNNNNTGG 1 cut(s) 670
XmaJI CCTAGG 1 cut(s) 1547
XmnI GAANNNNTTC 1 cut(s) 325
XspI CTAG 4 cut(s) 330, 540, 1452, 1548
ZrmI AGTACT 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.