Rorug04G0257800

RNA recognition motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002110
Physical Location & Seq
Reverse (-)
43111303 .. 43112381
1079 bp
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UTR
Exon/CDS
Intron
Rorug04G0257800.1

Sequence Viewer

Length: 450 bp
ATGTATTGCGCTTTGAAAAGCCTTAATGGTGTTGCTTGGAGATACGATTATATGTACTTGTTTGTTTTCCAGTTCGGTGTTTCGGAATTGCTTTGCTTTGTGCATCTGCACCTGGGTTTTGCTTGCTTTGTAAAAATTTATCTAGCTTTGAGAAGCTCAGGATTTGTTGTCTTTGCGGAAGTAATCAACTCATTCATCAAGATGCAGAACGAAGAGGGTCAAATCACTGAGCTCTACATTCCCAGGAAATGCTCAGCAACTAACAGGCTGATCACCTCGAAGGATCATGCATCTGTTCAGATCAACATCGGGCATCTTGATGAGAATGGCATCTATACTGGCCAGTTCTCCACTTTTGCTCTTTGTGGTTACGTCCGTGCTCAGGGAGATGCTGACAGTGGTCTCGACCGACTCTGGCAGAAGAAGAAAGCAGAAGTGAAACAACAGTAG

Protein Analysis

149

Amino Acids

16.98

Weight (kDa)

8.26

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21e PF01249 68 - 143 3.6e-37 Ribosomal protein S21e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 176
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 1 cut(s) 135
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 1 cut(s) 382
AgsI TTSAA 1 cut(s) 16
AjnI CCWGG 2 cut(s) 111, 242
AluBI AGCT 3 cut(s) 146, 156, 232
AluI AGCT 3 cut(s) 146, 156, 232
Alw21I GWGCWC 2 cut(s) 234, 382
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 1 cut(s) 291
AoxI GGCC 1 cut(s) 340
ApoI RAATTY 1 cut(s) 135
AspLEI GCGC 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 265
BalI TGGCCA 1 cut(s) 342
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 2 cut(s) 234, 382
BciT130I CCWGG 2 cut(s) 113, 244
BclI TGATCA 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 1 cut(s) 143
BlpI GCTNAGC 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 113, 244
BmrFI CCNGG 2 cut(s) 113, 244
BmsI GCATC 6 cut(s) 112, 192, 299, 322, 339, 379
BoxI GACNNNNGTC 1 cut(s) 399
Bpu10I CCTNAGC 2 cut(s) 157, 381
Bpu1102I GCTNAGC 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 407
BsaJI CCNNGG 2 cut(s) 112, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 382
Bse1I ACTGG 3 cut(s) 70, 343, 343
Bse8I GATNNNNATC 1 cut(s) 305
BseBI CCWGG 2 cut(s) 113, 244
BseDI CCNNGG 2 cut(s) 112, 242
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 382
BseMII CTCAG 4 cut(s) 171, 219, 267, 395
BseNI ACTGG 3 cut(s) 70, 343, 343
BsgI GTGCAG 1 cut(s) 92
Bsh1285I CGRYCG 1 cut(s) 409
BshFI GGCC 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 234, 382
BslI CCNNNNNNNGG 1 cut(s) 382
BsmAI GTCTC 1 cut(s) 407
BsnI GGCC 1 cut(s) 342
Bso31I GGTCTC 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 234, 382
Bsp143I GATC 3 cut(s) 270, 283, 300
Bsp1720I GCTNAGC 1 cut(s) 253
BspACI CCGC 1 cut(s) 176
BspANI GGCC 1 cut(s) 342
BspCNI CTCAG 4 cut(s) 170, 220, 266, 394
BspPI GGATC 1 cut(s) 291
BspTNI GGTCTC 1 cut(s) 407
BsrI ACTGG 3 cut(s) 70, 343, 343
BssECI CCNNGG 2 cut(s) 112, 242
BssMI GATC 3 cut(s) 270, 283, 300
Bst2UI CCWGG 2 cut(s) 113, 244
Bst4CI ACNGT 2 cut(s) 398, 447
Bst6I CTCTTC 1 cut(s) 207
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 4 cut(s) 157, 228, 253, 381
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 3 cut(s) 273, 286, 303
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 3 cut(s) 270, 283, 300
BstMCI CGRYCG 1 cut(s) 409
BstNI CCWGG 2 cut(s) 113, 244
BstPAI GACNNNNGTC 1 cut(s) 399
BstSCI CCNGG 2 cut(s) 111, 242
BsuRI GGCC 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 225, 403
Cac8I GCNNGC 1 cut(s) 124
CfoI GCGC 1 cut(s) 11
Csp6I GTAC 1 cut(s) 55
CviAII CATG 1 cut(s) 287
CviJI RGCY 6 cut(s) 21, 146, 156, 232, 268, 342
CviKI_1 RGCY 6 cut(s) 21, 146, 156, 232, 268, 342
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 4 cut(s) 157, 228, 253, 381
DpnI GATC 3 cut(s) 272, 285, 302
DpnII GATC 3 cut(s) 270, 283, 300
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 407
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 111, 242
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 1 cut(s) 290
FaiI YATR 4 cut(s) 51, 53, 288, 336
FatI CATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 10
HaeIII GGCC 1 cut(s) 342
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 1 cut(s) 411
HphI GGTGA 1 cut(s) 265
Hpy188I TCNGA 2 cut(s) 85, 300
Hpy188III TCNNGA 4 cut(s) 159, 199, 317, 404
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 2 cut(s) 398, 447
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 103, 109, 205, 290
HpyF3I CTNAG 4 cut(s) 157, 228, 253, 381
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 270
Kzo9I GATC 3 cut(s) 270, 283, 300
LweI GCATC 6 cut(s) 112, 192, 299, 322, 339, 379
MaeI CTAG 1 cut(s) 143
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 368
MalI GATC 3 cut(s) 272, 285, 302
MboI GATC 3 cut(s) 270, 283, 300
MboII GAAGA 3 cut(s) 224, 433, 436
MhlI GDGCHC 2 cut(s) 234, 382
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 2 cut(s) 86, 135
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 1 cut(s) 405
MnlI CCTC 2 cut(s) 208, 286
Mox20I TGGCCA 1 cut(s) 342
Mph1103I ATGCAT 1 cut(s) 292
MscI TGGCCA 1 cut(s) 342
MseI TTAA 1 cut(s) 24
MslI CAYNNNNRTG 2 cut(s) 200, 318
Msp20I TGGCCA 1 cut(s) 342
MspR9I CCNGG 2 cut(s) 113, 244
MvaI CCWGG 2 cut(s) 113, 244
NdeII GATC 3 cut(s) 270, 283, 300
NlaIII CATG 1 cut(s) 290
NsiI ATGCAT 1 cut(s) 292
PleI GAGTC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 405
PshAI GACNNNNGTC 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 111, 242
PspGI CCWGG 2 cut(s) 111, 242
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 2 cut(s) 200, 318
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 1 cut(s) 24
Sau3AI GATC 3 cut(s) 270, 283, 300
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 2 cut(s) 113, 244
SduI GDGCHC 2 cut(s) 234, 382
SetI ASST 6 cut(s) 114, 148, 158, 234, 278, 375
SfaNI GCATC 6 cut(s) 112, 192, 299, 322, 339, 379
SmiMI CAYNNNNRTG 2 cut(s) 200, 318
Sse9I AATT 2 cut(s) 86, 135
SsiI CCGC 1 cut(s) 176
SspMI CTAG 1 cut(s) 143
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 111, 242
TaaI ACNGT 2 cut(s) 398, 447
TaiI ACGT 1 cut(s) 375
TaqI TCGA 2 cut(s) 278, 405
TaqII GACCGA 1 cut(s) 423
TasI AATT 2 cut(s) 86, 135
TatI WGTACW 1 cut(s) 54
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TscAI CASTG 2 cut(s) 232, 403
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 2 cut(s) 232, 403
XapI RAATTY 1 cut(s) 135
XspI CTAG 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.