FvH4_4g26650

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011786
Physical Location & Seq
Forward (+)
27969991 .. 27972881
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26650.t2

Sequence Viewer

Length: 1593 bp
ATGGAGCCACCGGCGGCGGCGGCGCCACCACTTCAGCCTCAGTACCCCGACTCACTTGACTCGTCGCCGCGCTCCCGCAATACCGAGTCCTGGGACGACCCGCTCCCGCCGTCGTCCAGGCTCCGGCTCATGTGCAGCTACGGCGGACACATCGTGCCACGGCCGCACGACAAGTCGTTGTGCTACGTCGGCGGCGACACTCGAATCGTGGTCGTGGATAGGAATACTTCGCTCTCGGACCTCTCAAATCGGCTGGCGAAGACGCTTTTGAACGGTCGGCCGTTCACGCTCAAGTATCAGCTTCCGAGCGAGGACCTGGACTCTTTGATCTCTGTGACGACGGATGAGGATTTGGATAATATGATCGACGAGTTTGACCGGACGGCGAGTCATAATTCGTCGGCGAAACCGGCGCGGATTCGGTTGTTTCTGTTTCCGTTGAAGCCGGATTCGTCGCAGTCGATGGGGCCGATGCTGGATATGCCGACAAAATCGGAGGACTGGTTCCTCAACGCGCTCAACGGCTCGTCTGGGATGCTCAACCGCGGCTTCTCCGACTCCGCCTCCGTCGATTGCTTGCTCGGGTTAGACGACGGCGGCGGCGCCGCGAACAATTCGGAATCGAGAGGAGACGCGGAGGCTTCTGCGGCGGCGAATTGCAAGAACGGGAGGCCGGACGTTCATTCTGTCCCCGATTCGCCGATGCTGGAGAACTCGTCGTCGTTTGGCTCAACCTCATCGTCTCCGTCGCTGGCGAATTTGCCTCCTATTCGGGTCCACGTGGAAGACGGCGGGGGTGGTAGTAGTCACGGCGGGAATAGAGCGCAGGATCAGAAGGTGGGGATTGAAGAGCAGTTTGCTCAGATGACGGTGGGGATGGTTGGGCAGCAGAAGCAAGATGACGGCGGCGGCTTTGTGATCTTGTCTTCAACGCCGCCGTTGCCGACTACTATTATGCCTTCCTCTGCGCCGGTGAGCTCTTCTTCGCCGCCTGATTACCTGAATCGGGTCGTCTCCGATGATGAGCGATCCGATCACGGTGGATATCGGAAACCGATTCCTCCTCAGCCTCAGCAGCAGCAGAGTCTGCCTCCTCAGTCTCAGCCCAAATCTACCGGACTTTTTGATTTGCCGTCGCCAGATTCAGTGTCAAGTGATCATAGTTTATCAAATGTTGCCGTGTCACGCCCAAAACCTGTAGTTTATCAAGAACCAGTTGCTCAGATACCCTCTGGAACCCCCACTAGGTTTCCTGCAAACCTTGTTGATTCTAAAGTTAATGTCTCTGATCCCAACACTCGGGTTCAGATGCAACAACAGGTGCAGGATTCCGGGTATGTATTACAAAACCAGTATGATCAACAGCTACAACAGCAGCAACTACTACAACAACAACTACAACAGCAGCAGCATACTACCCTGTATATCCTTCTCAGCAGCAGCACCACCAGCAGCAACAGCAGCAACAACAGCAGCAACACCACCACCATCAGCTTGATCAGCAGCAGCAATACCAGGTTTATTACATGCCCGCAAGACAGGCCCCACCCTACAGCAACTTGCCCCTGCAGCAATCGCTGCAGTCCAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

531

Amino Acids

57.03

Weight (kDa)

5.02

Isoelectric Point (pI)

64.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 56 - 146 9.6e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01190
fragaria_vesca FvH4_4g26650 FvH4_4g26650 FvH4_4g26650
malus_domestica MD13G1034900.v1.1 MD16G1036800.v1.1
prunus_persica Prupe.1G317300_v2.0.a1
pyrus_communis pycom13g03050 pycom16g03170
rosa_chinensis RchiOBHm_Chr4g0434361
rosa_laevigata RLG00000006680
rosa_multiflora Rmu_sc0016328.1_g000003
rosa_roxburghii Rroxscaffold_5G00375720
rosa_rugosa Rorug04G0276300
rosa_samantha Rh4AG331400 Rh4BG339300 Rh4CG354400 Rh4DG334400
rosa_wichuraiana Rw4G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 22, 602
AccBSI CCGCTC 1 cut(s) 103
AccII CGCG 6 cut(s) 70, 415, 515, 546, 608, 635
AclWI GGATC 3 cut(s) 837, 1023, 1283
AcoI YGGCCR 2 cut(s) 161, 278
AcsI RAATTY 1 cut(s) 757
AcuI CTGAAG 1 cut(s) 17
AcvI CACGTG 1 cut(s) 781
AcyI GRCGYC 2 cut(s) 23, 603
AdeI CACNNNGTG 1 cut(s) 154
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 5 cut(s) 90, 123, 1006, 1245, 1299
AgsI TTSAA 4 cut(s) 271, 442, 848, 930
AhdI GACNNNNNGTC 1 cut(s) 387
AjnI CCWGG 4 cut(s) 89, 116, 315, 1514
AloI GAACNNNNNNTCC 2 cut(s) 488, 520
AluBI AGCT 5 cut(s) 138, 301, 978, 1366, 1494
AluI AGCT 5 cut(s) 138, 301, 978, 1366, 1494
Alw21I GWGCWC 1 cut(s) 980
Alw26I GTCTC 5 cut(s) 624, 747, 1018, 1104, 1288
AlwI GGATC 3 cut(s) 837, 1023, 1283
AlwNI CAGNNNCTG 1 cut(s) 1087
Ama87I CYCGRG 2 cut(s) 581, 1299
AoxI GGCC 5 cut(s) 161, 278, 467, 671, 1541
ApoI RAATTY 1 cut(s) 757
AspLEI GCGC 7 cut(s) 25, 72, 415, 517, 605, 826, 970
AspS9I GGNCC 5 cut(s) 238, 313, 467, 775, 1542
AsuC2I CCSGG 1 cut(s) 1333
AsuHPI GGTGA 1 cut(s) 985
AvaI CYCGRG 2 cut(s) 581, 1299
AvaII GGWCC 3 cut(s) 238, 313, 775
BanI GGYRCC 2 cut(s) 22, 602
BanII GRGCYC 1 cut(s) 980
BbrPI CACGTG 1 cut(s) 781
BbsI GAAGAC 3 cut(s) 266, 792, 918
Bbv12I GWGCWC 1 cut(s) 980
BbvCI CCTCAGC 2 cut(s) 1065, 1071
BccI CCATC 3 cut(s) 457, 871, 1496
BciT130I CCWGG 4 cut(s) 91, 118, 317, 1516
BclI TGATCA 3 cut(s) 1156, 1357, 1497
BcnI CCSGG 1 cut(s) 1333
BcoDI GTCTC 5 cut(s) 624, 747, 1018, 1104, 1288
BfaI CTAG 1 cut(s) 1245
BfmI CTRYAG 4 cut(s) 1197, 1551, 1567, 1579
BfoI RGCGCY 2 cut(s) 26, 606
Bme1390I CCNGG 5 cut(s) 91, 118, 317, 1333, 1516
Bme18I GGWCC 3 cut(s) 238, 313, 775
BmeRI GACNNNNNGTC 1 cut(s) 387
BmeT110I CYCGRG 2 cut(s) 581, 1299
BmgT120I GGNCC 5 cut(s) 238, 313, 467, 775, 1542
BmiI GGNNCC 9 cut(s) 6, 24, 122, 468, 506, 604, 776, 1237, 1544
BmrFI CCNGG 5 cut(s) 91, 118, 317, 1333, 1516
BmsI GCATC 4 cut(s) 462, 525, 693, 1299
BpiI GAAGAC 3 cut(s) 266, 792, 918
BplI GAGNNNNNCTC 2 cut(s) 1075, 1107
BpmI CTGGAG 1 cut(s) 728
Bpu10I CCTNAGC 2 cut(s) 1065, 1071
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 1333
BsaAI YACGTR 1 cut(s) 781
BsaHI GRCGYC 2 cut(s) 23, 603
BsaJI CCNNGG 3 cut(s) 90, 158, 544
BsaWI WCCGGW 2 cut(s) 378, 1115
BsaXI ACNNNNNCTCC 6 cut(s) 488, 518, 548, 578, 701, 731
Bsc4I CCNNNNNNNGG 5 cut(s) 90, 123, 1006, 1245, 1299
Bse118I RCCGGY 3 cut(s) 10, 409, 970
Bse1I ACTGG 3 cut(s) 506, 1214, 1351
BseBI CCWGG 4 cut(s) 91, 118, 317, 1516
BseDI CCNNGG 3 cut(s) 90, 158, 544
BseGI GGATG 3 cut(s) 349, 540, 882
BseLI CCNNNNNNNGG 5 cut(s) 90, 123, 1006, 1245, 1299
BseMII CTCAG 8 cut(s) 53, 875, 1079, 1085, 1109, 1115, 1235, 1447
BseNI ACTGG 3 cut(s) 506, 1214, 1351
BseRI GAGGAG 3 cut(s) 642, 1053, 1083
BseX3I CGGCCG 2 cut(s) 161, 278
BsgI GTGCAG 2 cut(s) 154, 1343
Bsh1236I CGCG 6 cut(s) 70, 415, 515, 546, 608, 635
Bsh1285I CGRYCG 3 cut(s) 164, 277, 281
BshFI GGCC 5 cut(s) 163, 280, 469, 673, 1543
BshNI GGYRCC 2 cut(s) 22, 602
BsiEI CGRYCG 3 cut(s) 164, 277, 281
BsiHKAI GWGCWC 1 cut(s) 980
BsiHKCI CYCGRG 2 cut(s) 581, 1299
BsiSI CCGG 9 cut(s) 11, 124, 379, 410, 446, 674, 971, 1116, 1332
BslFI GGGAC 2 cut(s) 107, 674
BslI CCNNNNNNNGG 5 cut(s) 90, 123, 1006, 1245, 1299
BsmAI GTCTC 5 cut(s) 624, 747, 1018, 1104, 1288
BsmBI CGTCTC 3 cut(s) 624, 747, 1018
BsmFI GGGAC 2 cut(s) 107, 674
BsnI GGCC 5 cut(s) 163, 280, 469, 673, 1543
BsoBI CYCGRG 2 cut(s) 581, 1299
Bsp1286I GDGCHC 1 cut(s) 980
BspANI GGCC 5 cut(s) 163, 280, 469, 673, 1543
BspCNI CTCAG 8 cut(s) 52, 874, 1078, 1084, 1108, 1114, 1234, 1446
BspFNI CGCG 6 cut(s) 70, 415, 515, 546, 608, 635
BspLI GGNNCC 9 cut(s) 6, 24, 122, 468, 506, 604, 776, 1237, 1544
BspMAI CTGCAG 2 cut(s) 1571, 1583
BspPI GGATC 3 cut(s) 837, 1023, 1283
BspQI GCTCTTC 2 cut(s) 843, 985
BspT107I GGYRCC 2 cut(s) 22, 602
BsrBI CCGCTC 1 cut(s) 103
BsrFI RCCGGY 3 cut(s) 10, 409, 970
BsrI ACTGG 3 cut(s) 506, 1214, 1351
BssAI RCCGGY 3 cut(s) 10, 409, 970
BssECI CCNNGG 3 cut(s) 90, 158, 544
BssNI GRCGYC 2 cut(s) 23, 603
Bst2UI CCWGG 4 cut(s) 91, 118, 317, 1516
Bst4CI ACNGT 3 cut(s) 275, 871, 1040
Bst6I CTCTTC 2 cut(s) 843, 985
BstACI GRCGYC 2 cut(s) 23, 603
BstAPI GCANNNNNTGC 2 cut(s) 1087, 1578
BstBAI YACGTR 1 cut(s) 781
BstC8I GCNNGC 4 cut(s) 255, 578, 753, 1532
BstDEI CTNAG 8 cut(s) 39, 861, 1065, 1071, 1095, 1101, 1221, 1433
BstDSI CCRYGG 2 cut(s) 158, 544
BstF5I GGATG 3 cut(s) 349, 540, 882
BstFNI CGCG 6 cut(s) 70, 415, 515, 546, 608, 635
BstH2I RGCGCY 2 cut(s) 26, 606
BstHHI GCGC 7 cut(s) 25, 72, 415, 517, 605, 826, 970
BstMAI GTCTC 5 cut(s) 624, 747, 1018, 1104, 1288
BstMCI CGRYCG 3 cut(s) 164, 277, 281
BstNI CCWGG 4 cut(s) 91, 118, 317, 1516
BstNSI RCATGY 1 cut(s) 1530
BstSCI CCNGG 5 cut(s) 89, 116, 315, 1331, 1514
BstSFI CTRYAG 4 cut(s) 1197, 1551, 1567, 1579
BstUI CGCG 6 cut(s) 70, 415, 515, 546, 608, 635
BstV2I GAAGAC 3 cut(s) 266, 792, 918
BstZI CGGCCG 2 cut(s) 161, 278
BsuRI GGCC 5 cut(s) 163, 280, 469, 673, 1543
BtgI CCRYGG 2 cut(s) 158, 544
BtsCI GGATG 3 cut(s) 349, 540, 882
BtsIMutI CAGTG 1 cut(s) 1152
Cac8I GCNNGC 4 cut(s) 255, 578, 753, 1532
CaiI CAGNNNCTG 1 cut(s) 1087
CfoI GCGC 7 cut(s) 25, 72, 415, 517, 605, 826, 970
Cfr10I RCCGGY 3 cut(s) 10, 409, 970
Cfr13I GGNCC 5 cut(s) 238, 313, 467, 775, 1542
Cfr42I CCGCGG 1 cut(s) 547
CseI GACGC 2 cut(s) 271, 641
CsiI ACCWGGT 1 cut(s) 1514
Csp6I GTAC 1 cut(s) 43
CviAII CATG 3 cut(s) 130, 1527, 1590
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 8 cut(s) 39, 861, 1065, 1071, 1095, 1101, 1221, 1433
DinI GGCGCC 2 cut(s) 24, 604
DraIII CACNNNGTG 1 cut(s) 154
DriI GACNNNNNGTC 1 cut(s) 387
EaeI YGGCCR 2 cut(s) 161, 278
EagI CGGCCG 2 cut(s) 161, 278
Eam1104I CTCTTC 2 cut(s) 843, 985
Eam1105I GACNNNNNGTC 1 cut(s) 387
EarI CTCTTC 2 cut(s) 843, 985
EciI GGCGGA 2 cut(s) 159, 550
Ecl136II GAGCTC 1 cut(s) 978
EclXI CGGCCG 2 cut(s) 161, 278
Eco24I GRGCYC 1 cut(s) 980
Eco32I GATATC 1 cut(s) 1046
Eco47I GGWCC 3 cut(s) 238, 313, 775
Eco52I CGGCCG 2 cut(s) 161, 278
Eco53kI GAGCTC 1 cut(s) 978
Eco57I CTGAAG 1 cut(s) 17
Eco72I CACGTG 1 cut(s) 781
Eco88I CYCGRG 2 cut(s) 581, 1299
EcoICRI GAGCTC 1 cut(s) 978
EcoO109I RGGNCCY 2 cut(s) 313, 1542
EcoRII CCWGG 4 cut(s) 89, 116, 315, 1514
EcoRV GATATC 1 cut(s) 1046
EcoT38I GRGCYC 1 cut(s) 980
EgeI GGCGCC 2 cut(s) 24, 604
EheI GGCGCC 2 cut(s) 24, 604
Esp3I CGTCTC 3 cut(s) 624, 747, 1018
FaeI CATG 3 cut(s) 133, 1530, 1593
FaqI GGGAC 2 cut(s) 107, 674
FatI CATG 3 cut(s) 129, 1526, 1589
FauI CCCGC 6 cut(s) 83, 108, 114, 785, 806, 1539
FbaI TGATCA 3 cut(s) 1156, 1357, 1497
FokI GGATG 3 cut(s) 356, 547, 889
FriOI GRGCYC 1 cut(s) 980
FspBI CTAG 1 cut(s) 1245
GlaI GCGC 7 cut(s) 24, 71, 414, 516, 604, 825, 969
GsuI CTGGAG 1 cut(s) 728
HaeII RGCGCY 2 cut(s) 26, 606
HaeIII GGCC 5 cut(s) 163, 280, 469, 673, 1543
HapII CCGG 9 cut(s) 11, 124, 379, 410, 446, 674, 971, 1116, 1332
HgaI GACGC 2 cut(s) 271, 641
HhaI GCGC 7 cut(s) 25, 72, 415, 517, 605, 826, 970
Hin1I GRCGYC 2 cut(s) 23, 603
Hin1II CATG 3 cut(s) 133, 1530, 1593
Hin6I GCGC 7 cut(s) 23, 70, 413, 515, 603, 824, 968
HinP1I GCGC 7 cut(s) 23, 70, 413, 515, 603, 824, 968
HpaII CCGG 9 cut(s) 11, 124, 379, 410, 446, 674, 971, 1116, 1332
HphI GGTGA 1 cut(s) 985
Hpy166II GTNNAC 2 cut(s) 285, 778
Hpy188III TCNNGA 3 cut(s) 624, 1208, 1233
Hpy8I GTNNAC 2 cut(s) 285, 778
HpyAV CCTTC 3 cut(s) 829, 969, 1439
HpyCH4III ACNGT 3 cut(s) 275, 871, 1040
HpyCH4IV ACGT 3 cut(s) 186, 678, 780
HpyCH4V TGCA 7 cut(s) 135, 660, 1256, 1312, 1324, 1569, 1581
HpyF3I CTNAG 8 cut(s) 39, 861, 1065, 1071, 1095, 1101, 1221, 1433
HpySE526I ACGT 3 cut(s) 186, 678, 780
Hsp92I GRCGYC 2 cut(s) 23, 603
Hsp92II CATG 3 cut(s) 133, 1530, 1593
HspAI GCGC 7 cut(s) 23, 70, 413, 515, 603, 824, 968
KasI GGCGCC 2 cut(s) 22, 602
Ksp22I TGATCA 3 cut(s) 1156, 1357, 1497
KspI CCGCGG 1 cut(s) 547
LguI GCTCTTC 2 cut(s) 843, 985
LmnI GCTCC 4 cut(s) 4, 77, 108, 126
LweI GCATC 4 cut(s) 462, 525, 693, 1299
MabI ACCWGGT 1 cut(s) 1514
MaeI CTAG 1 cut(s) 1245
MaeII ACGT 3 cut(s) 186, 678, 780
MaeIII GTNAC 3 cut(s) 334, 806, 1182
MbiI CCGCTC 1 cut(s) 103
MboII GAAGA 6 cut(s) 271, 797, 860, 918, 972, 975
MhlI GDGCHC 1 cut(s) 980
MluCI AATT 4 cut(s) 394, 613, 655, 757
Mly113I GGCGCC 2 cut(s) 23, 603
MlyI GAGTC 7 cut(s) 44, 53, 95, 314, 397, 551, 1093
MmeI TCCRAC 1 cut(s) 579
MseI TTAA 1 cut(s) 1278
MspA1I CMGCKG 1 cut(s) 546
MspI CCGG 9 cut(s) 11, 124, 379, 410, 446, 674, 971, 1116, 1332
MspR9I CCNGG 5 cut(s) 91, 118, 317, 1333, 1516
MvaI CCWGG 4 cut(s) 91, 118, 317, 1516
MvnI CGCG 6 cut(s) 70, 415, 515, 546, 608, 635
NarI GGCGCC 2 cut(s) 23, 603
NciI CCSGG 1 cut(s) 1333
NlaIII CATG 3 cut(s) 133, 1530, 1593
NlaIV GGNNCC 9 cut(s) 6, 24, 122, 468, 506, 604, 776, 1237, 1544
NmuCI GTSAC 3 cut(s) 334, 806, 1182
NspI RCATGY 1 cut(s) 1530
PciSI GCTCTTC 2 cut(s) 843, 985
PcsI WCGNNNNNNNCGW 4 cut(s) 192, 458, 467, 588
PleI GAGTC 7 cut(s) 44, 53, 94, 314, 396, 551, 1092
PluTI GGCGCC 2 cut(s) 26, 606
PmaCI CACGTG 1 cut(s) 781
PmlI CACGTG 1 cut(s) 781
PpsI GAGTC 7 cut(s) 44, 53, 94, 314, 396, 551, 1092
Ppu21I YACGTR 1 cut(s) 781
PpuMI RGGWCCY 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 980
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 4 cut(s) 89, 116, 315, 1514
PspCI CACGTG 1 cut(s) 781
PspGI CCWGG 4 cut(s) 89, 116, 315, 1514
PspN4I GGNNCC 9 cut(s) 6, 24, 122, 468, 506, 604, 776, 1237, 1544
PspPI GGNCC 5 cut(s) 238, 313, 467, 775, 1542
PspPPI RGGWCCY 1 cut(s) 313
PstI CTGCAG 2 cut(s) 1571, 1583
PstNI CAGNNNCTG 1 cut(s) 1087
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
SacI GAGCTC 1 cut(s) 980
SacII CCGCGG 1 cut(s) 547
SapI GCTCTTC 2 cut(s) 843, 985
SaqAI TTAA 1 cut(s) 1278
Sau96I GGNCC 5 cut(s) 238, 313, 467, 775, 1542
SchI GAGTC 7 cut(s) 44, 53, 95, 314, 397, 551, 1093
ScrFI CCNGG 5 cut(s) 91, 118, 317, 1333, 1516
SduI GDGCHC 1 cut(s) 980
SexAI ACCWGGT 1 cut(s) 1514
SfaNI GCATC 4 cut(s) 462, 525, 693, 1299
SfcI CTRYAG 4 cut(s) 1197, 1551, 1567, 1579
SfoI GGCGCC 2 cut(s) 24, 604
Sfr303I CCGCGG 1 cut(s) 547
SgrAI CRCCGGYG 2 cut(s) 10, 970
SgrBI CCGCGG 1 cut(s) 547
SinI GGWCC 3 cut(s) 238, 313, 775
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 4 cut(s) 394, 613, 655, 757
SspDI GGCGCC 2 cut(s) 22, 602
SspMI CTAG 1 cut(s) 1245
SstI GAGCTC 1 cut(s) 980
StyD4I CCNGG 5 cut(s) 89, 116, 315, 1331, 1514
TaaI ACNGT 3 cut(s) 275, 871, 1040
TaiI ACGT 3 cut(s) 189, 681, 783
TaqI TCGA 5 cut(s) 202, 366, 461, 570, 623
TasI AATT 4 cut(s) 394, 613, 655, 757
Tru1I TTAA 1 cut(s) 1278
Tru9I TTAA 1 cut(s) 1278
TscAI CASTG 1 cut(s) 1152
TseFI GTSAC 3 cut(s) 334, 806, 1182
Tsp45I GTSAC 3 cut(s) 334, 806, 1182
TspDTI ATGAA 1 cut(s) 671
TspGWI ACGGA 4 cut(s) 356, 426, 556, 735
TspRI CASTG 1 cut(s) 1152
VpaK11BI GGWCC 3 cut(s) 238, 313, 775
XapI RAATTY 1 cut(s) 757
XceI RCATGY 1 cut(s) 1530
XspI CTAG 1 cut(s) 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.