MD13G1034900.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011786
Physical Location & Seq
Forward (+)
2455566 .. 2458479
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1034900.v1.1.491

Sequence Viewer

Length: 2259 bp
ATGGACCCACCACCAGCGGCGCCCCCGCTGCAATCCCACAACCTCAACTACGCCGACTCCCTCGACTCTTCCCCTCGCTCCCGCAACACCGACTCATGGGACGAGCCTCAACCCCCCACCACCAGGCTCCGCCTTATGTGCAGCTACGGCGGCCACATCGTCCCCCGCCCCCACGACAAGTCGCTATGCTACGTCGGAGGCGACACCCGAATCGTCGTCGTCGACCGCCAGACCTCCCTCTCCGACCTCTCCAACCGCCTCTCCAAGACCCTCCTCAACGGCCGACCCTTCACCCTCAAGTACCAGCTCCCCAGCGAGGACCTCGACTCCTTAATCTCCCTCACCACCGACGAGGACCTCGACAACATGATCGACGAGTACGACCGCACGGCCAACCACAGCGGCGCTTCGTCCAAACCCTCGCGCCTCCGTCTCTTTCTCTTCCCCCTGAAGCCCGAGTCGTCGCAATCGATGGGGCCTATTCTCGATATTTCAGCCAATAAGTCCGAGGACTGGTTCTTGAACGCGCTCAATGGCGCGTCCGGTATGCTCAATCGGGGCTTCTCCGACTCCGCCTCCGTTAACTGCTTGCTGGGTCTCGACGACGATTCGATTTCCGGCGGTGCTGCCAATAATTTGGAATCCGGTTCGAGAGACGTGGAGGCTTCGGTTCCTGCGAATGGCAAGAATGCCAAACAGGGCGGGGGTGTCGGCGGTGGGAACGGGCAGGACGTTCAATCTGTCCCCGATTCGCCGATGCTTGAGAACTCCTCGTCGTTTGGGTCCACGTCATCGTCGCCATCGCTTGCGAACTTGCCACCGATTCGGGTCCACGTGGAAGATGGCGGCGGCGGTGGAGGTGGAGGCGGGATTGGAGTGCAGGATCAGAAGATGGGGATTGAAGAGCAGTTCGCTCAGATGACTGTTGGTGGTGGTGTTGGGCAGAAGCAAGACGACGGTGCCTTCGCGGTCCTTTCATCACCTCCTCCGATGCCCACTACCATCGTGGCATCCGCCGCGCCGCTGAGCACGGCCACCAATTACTTGAATCGCGTAGTTTCGGACGATGAGCGATCCGATCACGGCGCCCCAGCTGGGCATCGGAAACCAGCGCCACCGCAGCCTCAGCTTCAGCCACTGACTCTGCCTCCTCAGTCTCAGCAGCCCAAATCTAGTGGCGTTCTTGATTTGCCCTCCCCAGATTCAGTTTCAAGTGACAATAGTTTATCAAATGCCATGTCTCGCCCAAAACCTGTAATTTATCAAGACCCAGTAGTTCAAATCCCCTCTGCAAACGCCAGGTTTCCAGCAAACCTCGTTGATCCGAAATTAAATCTTTCCGATCCAACCACTCGGATTCAGATGCAACAACAGGTTCACGATTCTGGGTACGTTTTGCAATCGCAATTCGATCATCAACAGCAGCAGCTGCAGCAACACCACCAACAACAAGCACAACAACAACACCACCAACAAGAAGCACAACAGCAATACCACCAACAACAAGCACAACAGCAACACCACCAACAACAAGCACAACAGCAACACCAACAACAACAAGCACAACAGCAACACCAACAACAACAAGCACAGCAGGCACATCAATTTATACACGCTGGAACACATTACATCCAGCACCACCCAGGTTCGGTCCCCATTCCGGCGTACTACCCGGTGTATCCGCCCCAGCAGCAACAGCAACACCATCATCATCCCCAGCTTGATCAGCAGCAGCAGCAGCAATACCAAGTTTATTACATGCCTGCCAGGCAACCACAACCTTACGCCACTTTGCCAGTTCAGCAATCGAATATCAGTGATTCTGCCACTCCTATTCCTTCAAGCCACTCCCAAACGCCTCCTAATCCTGCCATTTCCTCCTCATCCACGGCTTACAGCCAAATGAGGAATGCCCAAATTGTCAAACCCGAAATGGCTGCCCCAGCAGGGGTGTACAGAACGGCAACCACAGCAGCTCCACCTCTAGTTCAAGTCCCCTCTGCTCAGCATCAGCAGCAATATGTCGGCTACCCCACCCAAATTCACCATCCGTCCCAATCGGCCGTTCCTTCTTCTGGGGGTAATGCCAGTTATGCCTATGAATATGCTACTGATCCCTCTCATGCGCAGATGTACTACACACAACCTCTGGCGCCCTCAATGCCTTCTCAGTACCAAACTATGACATCTGCGGCCGCCAGGGTGCTGCCAGAAATCTCTGCTCAGCTTCCCACCGACAACATCAAGCAGCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

753

Amino Acids

81.64

Weight (kDa)

5.88

Isoelectric Point (pI)

72.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 58 - 149 1.2e-17 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01190
fragaria_vesca FvH4_4g26650 FvH4_4g26650 FvH4_4g26650
malus_domestica MD13G1034900.v1.1 MD16G1036800.v1.1
prunus_persica Prupe.1G317300_v2.0.a1
pyrus_communis pycom13g03050 pycom16g03170
rosa_chinensis RchiOBHm_Chr4g0434361
rosa_laevigata RLG00000006680
rosa_multiflora Rmu_sc0016328.1_g000003
rosa_roxburghii Rroxscaffold_5G00375720
rosa_rugosa Rorug04G0276300
rosa_samantha Rh4AG331400 Rh4BG339300 Rh4CG354400 Rh4DG334400
rosa_wichuraiana Rw4G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2127
AccB1I GGYRCC 4 cut(s) 19, 959, 1085, 2152
AccI GTMKAC 1 cut(s) 222
AccII CGCG 6 cut(s) 424, 527, 539, 968, 1019, 1053
AclWI GGATC 5 cut(s) 891, 1068, 1316, 1337, 2108
AcoI YGGCCR 6 cut(s) 151, 280, 390, 1032, 2061, 2193
AcsI RAATTY 1 cut(s) 2040
AcuI CTGAAG 2 cut(s) 470, 1115
AcvI CACGTG 1 cut(s) 835
AcyI GRCGYC 3 cut(s) 20, 1086, 2153
AfaI GTAC 7 cut(s) 302, 380, 1391, 1667, 1955, 2135, 2174
AgsI TTSAA 8 cut(s) 523, 737, 902, 1048, 1212, 1280, 1842, 1991
AjiI CACGTC 2 cut(s) 658, 789
AjnI CCWGG 5 cut(s) 122, 1298, 1642, 1766, 2198
AjuI GAANNNNNNNTTGG 2 cut(s) 2049, 2081
AluBI AGCT 8 cut(s) 144, 307, 1094, 1129, 1429, 1720, 1976, 2227
AluI AGCT 8 cut(s) 144, 307, 1094, 1129, 1429, 1720, 1976, 2227
Alw21I GWGCWC 1 cut(s) 1031
Alw26I GTCTC 5 cut(s) 437, 602, 648, 1161, 1245
AlwI GGATC 5 cut(s) 891, 1068, 1316, 1337, 2108
AlwNI CAGNNNCTG 2 cut(s) 1138, 1429
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 7 cut(s) 151, 280, 390, 476, 1032, 2061, 2193
ApoI RAATTY 1 cut(s) 2040
ArsI GACNNNNNNTTYG 4 cut(s) 947, 979, 1223, 1255
AspS9I GGNCC 8 cut(s) 4, 319, 355, 476, 783, 829, 970, 1651
AsuC2I CCSGG 1 cut(s) 1673
AsuHPI GGTGA 4 cut(s) 283, 334, 972, 2036
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 7 cut(s) 4, 319, 355, 783, 829, 970, 1651
BanI GGYRCC 4 cut(s) 19, 959, 1085, 2152
BbrPI CACGTG 1 cut(s) 835
Bbv12I GWGCWC 1 cut(s) 1031
BbvCI CCTCAGC 1 cut(s) 1125
BccI CCATC 7 cut(s) 466, 808, 836, 886, 1010, 1713, 2055
BceAI ACGGC 8 cut(s) 163, 295, 405, 1047, 1099, 1905, 1977, 2048
BcgI CGANNNNNNTGC 2 cut(s) 1919, 1953
BciT130I CCWGG 5 cut(s) 124, 1300, 1644, 1768, 2200
BciVI GTATCC 1 cut(s) 1689
BclI TGATCA 1 cut(s) 1723
BcnI CCSGG 1 cut(s) 1673
BcoDI GTCTC 5 cut(s) 437, 602, 648, 1161, 1245
BfaI CTAG 2 cut(s) 1173, 1985
BfmI CTRYAG 1 cut(s) 1430
BfoI RGCGCY 5 cut(s) 23, 408, 1089, 1115, 2156
BfuI GTATCC 1 cut(s) 1689
BglI GCCNNNNNGGC 1 cut(s) 1768
BlpI GCTNAGC 3 cut(s) 1025, 2004, 2223
Bme1390I CCNGG 6 cut(s) 124, 1300, 1644, 1673, 1768, 2200
Bme18I GGWCC 7 cut(s) 4, 319, 355, 783, 829, 970, 1651
BmeT110I CYCGRG 1 cut(s) 455
BmgBI CACGTC 2 cut(s) 658, 789
BmgT120I GGNCC 8 cut(s) 4, 319, 355, 476, 783, 829, 970, 1651
BmrFI CCNGG 6 cut(s) 124, 1300, 1644, 1673, 1768, 2200
BmrI ACTGGG 1 cut(s) 1265
BmsI GCATC 6 cut(s) 747, 981, 1019, 1108, 1353, 2017
BmuI ACTGGG 1 cut(s) 1265
BplI GAGNNNNNCTC 2 cut(s) 755, 787
Bpu10I CCTNAGC 1 cut(s) 1125
Bpu1102I GCTNAGC 3 cut(s) 1025, 2004, 2223
BpuEI CTTGAG 2 cut(s) 281, 782
BpuMI CCSGG 1 cut(s) 1673
Bsa29I ATCGAT 1 cut(s) 470
BsaAI YACGTR 1 cut(s) 835
BsaHI GRCGYC 3 cut(s) 20, 1086, 2153
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 4 cut(s) 507, 1642, 1887, 2199
BsaWI WCCGGW 2 cut(s) 542, 644
Bse1I ACTGG 4 cut(s) 518, 1271, 1796, 2088
BseBI CCWGG 5 cut(s) 124, 1300, 1644, 1768, 2200
BseCI ATCGAT 1 cut(s) 470
BseDI CCNNGG 4 cut(s) 507, 1642, 1887, 2199
BseGI GGATG 5 cut(s) 1010, 1629, 1711, 1883, 2047
BseMII CTCAG 8 cut(s) 929, 1016, 1139, 1166, 1172, 2018, 2183, 2237
BseNI ACTGG 4 cut(s) 518, 1271, 1796, 2088
BseRI GAGGAG 5 cut(s) 263, 760, 975, 1140, 1870
BseX3I CGGCCG 3 cut(s) 280, 2061, 2193
BseYI CCCAGC 7 cut(s) 311, 592, 1090, 1094, 1686, 1716, 1942
BsgI GTGCAG 2 cut(s) 160, 899
Bsh1236I CGCG 6 cut(s) 424, 527, 539, 968, 1019, 1053
Bsh1285I CGRYCG 5 cut(s) 226, 283, 385, 2064, 2196
BshFI GGCC 7 cut(s) 153, 282, 392, 478, 1034, 2063, 2195
BshNI GGYRCC 4 cut(s) 19, 959, 1085, 2152
BshVI ATCGAT 1 cut(s) 470
BsiEI CGRYCG 5 cut(s) 226, 283, 385, 2064, 2196
BsiHKAI GWGCWC 1 cut(s) 1031
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 5 cut(s) 543, 618, 645, 1661, 1673
BslFI GGGAC 6 cut(s) 113, 146, 728, 1637, 1979, 2038
BsmAI GTCTC 5 cut(s) 437, 602, 648, 1161, 1245
BsmBI CGTCTC 2 cut(s) 437, 648
BsmFI GGGAC 6 cut(s) 113, 146, 728, 1637, 1979, 2038
BsmI GAATGC 2 cut(s) 694, 1915
BsnI GGCC 7 cut(s) 153, 282, 392, 478, 1034, 2063, 2195
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 455
Bsp1286I GDGCHC 1 cut(s) 1031
Bsp1407I TGTACA 1 cut(s) 1953
Bsp143I GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
Bsp1720I GCTNAGC 3 cut(s) 1025, 2004, 2223
BspANI GGCC 7 cut(s) 153, 282, 392, 478, 1034, 2063, 2195
BspCNI CTCAG 8 cut(s) 928, 1017, 1138, 1165, 1171, 2017, 2182, 2236
BspDI ATCGAT 1 cut(s) 470
BspFNI CGCG 6 cut(s) 424, 527, 539, 968, 1019, 1053
BspMAI CTGCAG 1 cut(s) 1434
BspPI GGATC 5 cut(s) 891, 1068, 1316, 1337, 2108
BspQI GCTCTTC 1 cut(s) 897
BspT107I GGYRCC 4 cut(s) 19, 959, 1085, 2152
BspTNI GGTCTC 1 cut(s) 602
BsrGI TGTACA 1 cut(s) 1953
BsrI ACTGG 4 cut(s) 518, 1271, 1796, 2088
BssECI CCNNGG 4 cut(s) 507, 1642, 1887, 2199
BssMI GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
BssNI GRCGYC 3 cut(s) 20, 1086, 2153
Bst2UI CCWGG 5 cut(s) 124, 1300, 1644, 1768, 2200
Bst4CI ACNGT 2 cut(s) 925, 959
Bst6I CTCTTC 3 cut(s) 73, 446, 897
BstACI GRCGYC 3 cut(s) 20, 1086, 2153
BstAPI GCANNNNNTGC 1 cut(s) 1429
BstAUI TGTACA 1 cut(s) 1953
BstBAI YACGTR 1 cut(s) 835
BstC8I GCNNGC 4 cut(s) 590, 807, 1596, 1764
BstDEI CTNAG 8 cut(s) 915, 1025, 1125, 1152, 1158, 2004, 2169, 2223
BstDSI CCRYGG 1 cut(s) 1887
BstF5I GGATG 5 cut(s) 1010, 1629, 1711, 1883, 2047
BstFNI CGCG 6 cut(s) 424, 527, 539, 968, 1019, 1053
BstH2I RGCGCY 5 cut(s) 23, 408, 1089, 1115, 2156
BstKTI GATC 9 cut(s) 372, 886, 1076, 1081, 1324, 1345, 1414, 1726, 2116
BstMAI GTCTC 5 cut(s) 437, 602, 648, 1161, 1245
BstMBI GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
BstMCI CGRYCG 5 cut(s) 226, 283, 385, 2064, 2196
BstNI CCWGG 5 cut(s) 124, 1300, 1644, 1768, 2200
BstNSI RCATGY 1 cut(s) 1762
BstSCI CCNGG 6 cut(s) 122, 1298, 1642, 1671, 1766, 2198
BstSFI CTRYAG 1 cut(s) 1430
BstUI CGCG 6 cut(s) 424, 527, 539, 968, 1019, 1053
BstXI CCANNNNNNTGG 1 cut(s) 637
BstZI CGGCCG 3 cut(s) 280, 2061, 2193
Bsu15I ATCGAT 1 cut(s) 470
BsuI GTATCC 1 cut(s) 1689
BsuRI GGCC 7 cut(s) 153, 282, 392, 478, 1034, 2063, 2195
BsuTUI ATCGAT 1 cut(s) 470
BtgI CCRYGG 1 cut(s) 1887
BtgZI GCGATG 1 cut(s) 786
BtrI CACGTC 2 cut(s) 658, 789
BtsCI GGATG 5 cut(s) 1010, 1629, 1711, 1883, 2047
BtsIMutI CAGTG 2 cut(s) 1136, 1822
Cac8I GCNNGC 4 cut(s) 590, 807, 1596, 1764
CaiI CAGNNNCTG 2 cut(s) 1138, 1429
CciNI GCGGCCGC 1 cut(s) 2193
Cfr13I GGNCC 8 cut(s) 4, 319, 355, 476, 783, 829, 970, 1651
ClaI ATCGAT 1 cut(s) 470
CseI GACGC 1 cut(s) 528
Csp6I GTAC 7 cut(s) 301, 379, 1390, 1666, 1954, 2134, 2173
CspCI CAANNNNNGTGG 2 cut(s) 1156, 1191
CviAII CATG 5 cut(s) 96, 367, 1237, 1759, 2123
CviQI GTAC 7 cut(s) 301, 379, 1390, 1666, 1954, 2134, 2173
DdeI CTNAG 8 cut(s) 915, 1025, 1125, 1152, 1158, 2004, 2169, 2223
DinI GGCGCC 3 cut(s) 21, 1087, 2154
DpnI GATC 9 cut(s) 371, 885, 1075, 1080, 1323, 1344, 1413, 1725, 2115
DpnII GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
EaeI YGGCCR 6 cut(s) 151, 280, 390, 1032, 2061, 2193
EagI CGGCCG 3 cut(s) 280, 2061, 2193
Eam1104I CTCTTC 3 cut(s) 73, 446, 897
EarI CTCTTC 3 cut(s) 73, 446, 897
EciI GGCGGA 4 cut(s) 119, 562, 1003, 1671
EclXI CGGCCG 3 cut(s) 280, 2061, 2193
Eco31I GGTCTC 1 cut(s) 602
Eco47I GGWCC 7 cut(s) 4, 319, 355, 783, 829, 970, 1651
Eco52I CGGCCG 3 cut(s) 280, 2061, 2193
Eco57I CTGAAG 2 cut(s) 470, 1115
Eco72I CACGTG 1 cut(s) 835
Eco88I CYCGRG 1 cut(s) 455
EcoO109I RGGNCCY 3 cut(s) 319, 355, 476
EcoRII CCWGG 5 cut(s) 122, 1298, 1642, 1766, 2198
EgeI GGCGCC 3 cut(s) 21, 1087, 2154
EheI GGCGCC 3 cut(s) 21, 1087, 2154
Esp3I CGTCTC 2 cut(s) 437, 648
FaeI CATG 5 cut(s) 99, 370, 1240, 1762, 2126
FaqI GGGAC 6 cut(s) 113, 146, 728, 1637, 1979, 2038
FatI CATG 5 cut(s) 95, 366, 1236, 1758, 2122
FauI CCCGC 5 cut(s) 33, 89, 173, 695, 860
FbaI TGATCA 1 cut(s) 1723
FblI GTMKAC 1 cut(s) 222
FokI GGATG 5 cut(s) 997, 1616, 1698, 1870, 2034
FspBI CTAG 2 cut(s) 1173, 1985
FspI TGCGCA 1 cut(s) 2127
GsaI CCCAGC 7 cut(s) 315, 596, 1094, 1098, 1690, 1720, 1946
HaeII RGCGCY 5 cut(s) 23, 408, 1089, 1115, 2156
HaeIII GGCC 7 cut(s) 153, 282, 392, 478, 1034, 2063, 2195
HapII CCGG 5 cut(s) 543, 618, 645, 1661, 1673
HgaI GACGC 1 cut(s) 528
Hin1I GRCGYC 3 cut(s) 20, 1086, 2153
Hin1II CATG 5 cut(s) 99, 370, 1240, 1762, 2126
HincII GTYRAC 2 cut(s) 223, 583
HindII GTYRAC 2 cut(s) 223, 583
HpaI GTTAAC 1 cut(s) 583
HpaII CCGG 5 cut(s) 543, 618, 645, 1661, 1673
HphI GGTGA 4 cut(s) 283, 334, 972, 2036
Hpy166II GTNNAC 6 cut(s) 223, 583, 786, 832, 1378, 1954
Hpy188III TCNNGA 7 cut(s) 485, 520, 599, 651, 1184, 1265, 1379
Hpy8I GTNNAC 6 cut(s) 223, 583, 786, 832, 1378, 1954
HpyAV CCTTC 5 cut(s) 298, 973, 1848, 2079, 2175
HpyCH4III ACNGT 2 cut(s) 925, 959
HpyCH4IV ACGT 6 cut(s) 192, 657, 732, 788, 834, 1392
HpyCH4V TGCA 7 cut(s) 31, 141, 880, 1292, 1366, 1399, 1432
HpyF3I CTNAG 8 cut(s) 915, 1025, 1125, 1152, 1158, 2004, 2169, 2223
HpySE526I ACGT 6 cut(s) 192, 657, 732, 788, 834, 1392
Hsp92I GRCGYC 3 cut(s) 20, 1086, 2153
Hsp92II CATG 5 cut(s) 99, 370, 1240, 1762, 2126
KasI GGCGCC 3 cut(s) 19, 1085, 2152
Ksp22I TGATCA 1 cut(s) 1723
KspAI GTTAAC 1 cut(s) 583
Kzo9I GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 4 cut(s) 83, 132, 312, 1981
LweI GCATC 6 cut(s) 747, 981, 1019, 1108, 1353, 2017
MaeI CTAG 2 cut(s) 1173, 1985
MaeII ACGT 6 cut(s) 192, 657, 732, 788, 834, 1392
MaeIII GTNAC 1 cut(s) 1214
MalI GATC 9 cut(s) 371, 885, 1075, 1080, 1323, 1344, 1413, 1725, 2115
MboI GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
MboII GAAGA 6 cut(s) 60, 433, 851, 901, 914, 2064
MhlI GDGCHC 1 cut(s) 1031
MluCI AATT 8 cut(s) 634, 1039, 1257, 1328, 1406, 1604, 1917, 2040
Mly113I GGCGCC 3 cut(s) 20, 1086, 2153
MlyI GAGTC 7 cut(s) 50, 59, 86, 320, 467, 563, 1135
MmeI TCCRAC 5 cut(s) 175, 267, 276, 591, 1370
MseI TTAA 3 cut(s) 332, 582, 1331
MspA1I CMGCKG 6 cut(s) 17, 28, 402, 1024, 1094, 1429
MspI CCGG 5 cut(s) 543, 618, 645, 1661, 1673
MspR9I CCNGG 6 cut(s) 124, 1300, 1644, 1673, 1768, 2200
Mva1269I GAATGC 2 cut(s) 694, 1915
MvaI CCWGG 5 cut(s) 124, 1300, 1644, 1768, 2200
MvnI CGCG 6 cut(s) 424, 527, 539, 968, 1019, 1053
NarI GGCGCC 3 cut(s) 20, 1086, 2153
NciI CCSGG 1 cut(s) 1673
NdeII GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
NlaIII CATG 5 cut(s) 99, 370, 1240, 1762, 2126
NmuCI GTSAC 1 cut(s) 1214
NotI GCGGCCGC 1 cut(s) 2193
NsbI TGCGCA 1 cut(s) 2127
NspI RCATGY 1 cut(s) 1762
PciSI GCTCTTC 1 cut(s) 897
PcsI WCGNNNNNNNCGW 7 cut(s) 198, 219, 357, 378, 467, 674, 729
PctI GAATGC 2 cut(s) 694, 1915
PleI GAGTC 7 cut(s) 50, 59, 86, 320, 466, 563, 1135
PluTI GGCGCC 3 cut(s) 23, 1089, 2156
PmaCI CACGTG 1 cut(s) 835
PmlI CACGTG 1 cut(s) 835
PpsI GAGTC 7 cut(s) 50, 59, 86, 320, 466, 563, 1135
Ppu21I YACGTR 1 cut(s) 835
PpuMI RGGWCCY 2 cut(s) 319, 355
Psp5II RGGWCCY 2 cut(s) 319, 355
Psp6I CCWGG 5 cut(s) 122, 1298, 1642, 1766, 2198
PspCI CACGTG 1 cut(s) 835
PspFI CCCAGC 7 cut(s) 311, 592, 1090, 1094, 1686, 1716, 1942
PspGI CCWGG 5 cut(s) 122, 1298, 1642, 1766, 2198
PspPI GGNCC 8 cut(s) 4, 319, 355, 476, 783, 829, 970, 1651
PspPPI RGGWCCY 2 cut(s) 319, 355
PstI CTGCAG 1 cut(s) 1434
PstNI CAGNNNCTG 2 cut(s) 1138, 1429
PvuII CAGCTG 2 cut(s) 1094, 1429
RsaI GTAC 7 cut(s) 302, 380, 1391, 1667, 1955, 2135, 2174
RsaNI GTAC 7 cut(s) 301, 379, 1390, 1666, 1954, 2134, 2173
SalI GTCGAC 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 897
SaqAI TTAA 3 cut(s) 332, 582, 1331
Sau3AI GATC 9 cut(s) 369, 883, 1073, 1078, 1321, 1342, 1411, 1723, 2113
Sau96I GGNCC 8 cut(s) 4, 319, 355, 476, 783, 829, 970, 1651
SchI GAGTC 7 cut(s) 50, 59, 86, 320, 467, 563, 1135
ScrFI CCNGG 6 cut(s) 124, 1300, 1644, 1673, 1768, 2200
SduI GDGCHC 1 cut(s) 1031
SfaNI GCATC 6 cut(s) 747, 981, 1019, 1108, 1353, 2017
SfcI CTRYAG 1 cut(s) 1430
SfoI GGCGCC 3 cut(s) 21, 1087, 2154
SinI GGWCC 7 cut(s) 4, 319, 355, 783, 829, 970, 1651
SmlI CTYRAG 2 cut(s) 296, 761
SmoI CTYRAG 2 cut(s) 296, 761
Sse9I AATT 8 cut(s) 634, 1039, 1257, 1328, 1406, 1604, 1917, 2040
SspDI GGCGCC 3 cut(s) 19, 1085, 2152
SspMI CTAG 2 cut(s) 1173, 1985
StyD4I CCNGG 6 cut(s) 122, 1298, 1642, 1671, 1766, 2198
TaaI ACNGT 2 cut(s) 925, 959
TaiI ACGT 6 cut(s) 195, 660, 735, 791, 837, 1395
TaqII GACCGA 1 cut(s) 1639
TasI AATT 8 cut(s) 634, 1039, 1257, 1328, 1406, 1604, 1917, 2040
TatI WGTACW 2 cut(s) 1953, 2133
TauI GCSGC 9 cut(s) 20, 153, 405, 849, 852, 1019, 1024, 2195, 2198
Tru1I TTAA 3 cut(s) 332, 582, 1331
Tru9I TTAA 3 cut(s) 332, 582, 1331
TscAI CASTG 2 cut(s) 1143, 1822
TseFI GTSAC 1 cut(s) 1214
Tsp45I GTSAC 1 cut(s) 1214
TspDTI ATGAA 2 cut(s) 966, 2115
TspGWI ACGGA 3 cut(s) 419, 568, 2040
TspRI CASTG 2 cut(s) 1143, 1822
VpaK11BI GGWCC 7 cut(s) 4, 319, 355, 783, 829, 970, 1651
XapI RAATTY 1 cut(s) 2040
XceI RCATGY 1 cut(s) 1762
XcmI CCANNNNNNNNNTGG 2 cut(s) 839, 1003
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 2 cut(s) 1173, 1985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.