Rw4G028740

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011786
Physical Location & Seq
Forward (+)
55373158 .. 55376087
2930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028740.1

Sequence Viewer

Length: 2145 bp
ATGGAGCCACCGGCGGCGGCGCCGCCAATCCAGACTCACTACGCCGACTCGCTGGACTCGTCGCCGAGGTCCCGTAACACCGACTCCTGGGACGACCCGCTCCCGCCGGCGTCCAGGCTCCGGCTGATGTGCAGCTACGGCGGCCACATCGTGCCGCGGCCGCACGACAAGTCGTTGTGCTACGTCGGCGGCGACACTCGAATCGTCGTCGTGGACAGGAACACTTCGCTTGCGGACCTCTCGAATCGTCTGGCGAAGACGCTTTTGAATAACCGGCCGTTCACGCTGAAATACCAGTTGCCGAGCGAGGACCTGGACTCGTTGATCTCCGTGACGACCGACGAGGATTTGGATAATATGATCGACGAGTTTGACCGGACGGCGAGCCACAATTCGTCGGCGAAGCCGGCGCGGATTCGGCTGTTTCTGTTTCCGCTGAAGCCGGATTCGTCGCAGTCGATGGGGCCCCTCGATATGCCGACCAAGTCGGAGGACTGGTTCTTCAACGCGCTCAACGGCTCGTCGGAGATACTCAACCGCGGCTTCTCCGACTCCGCCTCCGTCAACTGCTTGCTCGGCCTAGACGACGACGGCGGCGGCGGCGGGGCGAACAATTCGGATTCGAGAGGCGGAGACGCGGAGGCTTCTGCGGCGGCGAATTGCAAGAACGGTAAACCGGACGTTCACTCTGTCCCCGATTCGCCGATGCTCGAGAACTCGTCGTCGTTTGGGTCAACATCGTCGTCTCCGTCGCTGGCGAATTTGCCTCCGATTCGGGTCCACGTGGAAGACAGCGGTGGGAGCAGTCACGGTGGCAATAGAGCGCAGGATCAGAAGGTTGGGATTGAAGAGCAGTTTGCTCAGATGACGGTGGGTTTGGTTGGGCAGCAGCAGAAGCAAGACGATAGCGGCGGCTTTGTGGTGCTGTCTTCACCGCCGCCGTTGCCGACTACTATTGTGCCTTCCTCTGCGCCGGTGAGTTCTTCTTCGGCCCCTGATTACCTGAATCGGATCGTCTCCGATGACGAACGATCCGATCACGGTGGATATCGGAAACCGCTTCCTCCTCAGCCTCAGCAACAGAGTGTACCGCCTCAGTCTCAGCAACAGAGTGTACCGCCTCAGTCTCAGCCCAAATCAACCGGAATTTTTGATTTGCCGTCGCCAGATTCAGTGTCAAGTGATCATAGTTTATCAAATGCTGCCATATCTCGCCCAAAACCTGTAATTTATCAAGAACCAGTTGCTCAAATACCCGCGGTAACCCCCACAAGGTTTCCTGCAAATCTTGTTGATCCGAAAGTTAATGTCTCCGATCCAAACACTCGGGTTCAGTTGCAACAACAGGTTCAGGATTCCGGGTATGTATTGCAAGCCCAATATGATCAACAAATACAACAGCAGCAACTACTACAACAACAACAACAACTACAACAACAGCAACAATTTATGCACCCTGGTGCACATTACATGCATCATCACCCTGCTGGGTCTGTGCCAATTCAGGCATACTACCCTGTATATCCTTCTCAGCAGCACCAGCAGCAACAGCACCAGCAGCAACAGCAACACCACCATCATCCTCAGCTCGATCAGCAGCAGCAATACCAGGTTTATTACATGCCTGCAAGACAGGCCCAAGCTTACAGCAACTTGCCCCTGCAGCAACCGCTGCAGTCCAACATTAGTGAAGCTGCTGCTGCTACTATTGCTTCTAGCCGGTCTCAACCGCCTCCCAATCCAATGCTCTCTCCTACCGCAGCTTACAGCCAGATAAGGAATGCCCCCATTGCCAAACCCGAAATTGCTGCTGGTGTGTACAGGACAACAACTACAGCTGCTCCTCCATCTCTGGTTCAAGTTCCCCCTACTCAGCATCAGCCTCAGCAACAATATGTTGGTTACGCCCAGGTTCATCACCCGTCTCAATCAACTGTTCCTACTTCGACAGGTGCTGCTAATTATGCCTATGAATATGCCGATGCTTCTCATGCTCAGCATGCCCAGGTATACTATACTCAGCCTCTAGCACCCGCAATGACTTCTCAGTACCAAACCATGACAGCTGCAGCTGCCATGGGGCTGCCGGAAGTTTCAACTCAGCTTCCTAATGACAACATCAAGCAGCAGCAGCAGCAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

714

Amino Acids

77.7

Weight (kDa)

5.66

Isoelectric Point (pI)

73.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 55 - 145 2e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01190
fragaria_vesca FvH4_4g26650 FvH4_4g26650 FvH4_4g26650
malus_domestica MD13G1034900.v1.1 MD16G1036800.v1.1
prunus_persica Prupe.1G317300_v2.0.a1
pyrus_communis pycom13g03050 pycom16g03170
rosa_chinensis RchiOBHm_Chr4g0434361
rosa_laevigata RLG00000006680
rosa_multiflora Rmu_sc0016328.1_g000003
rosa_roxburghii Rroxscaffold_5G00375720
rosa_rugosa Rorug04G0276300
rosa_samantha Rh4AG331400 Rh4BG339300 Rh4CG354400 Rh4DG334400
rosa_wichuraiana Rw4G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 2010
AccII CGCG 6 cut(s) 157, 412, 509, 540, 638, 1259
AclWI GGATC 5 cut(s) 837, 1019, 1026, 1289, 1310
AcoI YGGCCR 3 cut(s) 142, 158, 275
AcsI RAATTY 2 cut(s) 760, 1146
AcuI CTGAAG 1 cut(s) 458
AcvI CACGTG 1 cut(s) 784
AcyI GRCGYC 2 cut(s) 20, 110
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 4 cut(s) 1089, 1116, 1820, 2051
AfiI CCNNNNNNNGG 3 cut(s) 87, 120, 1272
AgsI TTSAA 5 cut(s) 268, 505, 848, 1859, 2097
AjnI CCWGG 7 cut(s) 86, 113, 312, 1456, 1608, 1908, 2004
AleI CACNNNNGTG 1 cut(s) 1458
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
AluBI AGCT 9 cut(s) 135, 1588, 1643, 1694, 1763, 1838, 2066, 2072, 2104
AluI AGCT 9 cut(s) 135, 1588, 1643, 1694, 1763, 1838, 2066, 2072, 2104
Alw21I GWGCWC 1 cut(s) 1465
Alw26I GTCTC 8 cut(s) 627, 750, 1021, 1104, 1131, 1315, 1728, 1929
Alw44I GTGCAC 1 cut(s) 1461
AlwI GGATC 5 cut(s) 837, 1019, 1026, 1289, 1310
AlwNI CAGNNNCTG 1 cut(s) 1955
Ama87I CYCGRG 2 cut(s) 710, 1326
AoxI GGCC 7 cut(s) 142, 158, 275, 464, 577, 990, 1635
ApaI GGGCCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 1461
ApoI RAATTY 2 cut(s) 760, 1146
ArsI GACNNNNNNTTYG 2 cut(s) 859, 891
AspLEI GCGC 5 cut(s) 22, 412, 511, 826, 973
AspS9I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1636
AsuC2I CCSGG 1 cut(s) 1360
AsuHPI GGTGA 4 cut(s) 924, 988, 1472, 1910
AvaI CYCGRG 2 cut(s) 710, 1326
AvaII GGWCC 4 cut(s) 69, 235, 310, 778
BaeGI GKGCMC 2 cut(s) 468, 1465
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 468
BbrPI CACGTG 1 cut(s) 784
BbsI GAAGAC 3 cut(s) 263, 795, 921
Bbv12I GWGCWC 1 cut(s) 1465
BbvCI CCTCAGC 4 cut(s) 1068, 1074, 1584, 1884
BccI CCATC 3 cut(s) 454, 1584, 1855
BceAI ACGGC 7 cut(s) 154, 262, 396, 532, 607, 925, 1144
BcgI CGANNNNNNTGC 2 cut(s) 1790, 1824
BciT130I CCWGG 7 cut(s) 88, 115, 314, 1458, 1610, 1910, 2006
BclI TGATCA 2 cut(s) 1183, 1384
BcnI CCSGG 1 cut(s) 1360
BcoDI GTCTC 8 cut(s) 627, 750, 1021, 1104, 1131, 1315, 1728, 1929
BfaI CTAG 3 cut(s) 581, 1716, 2027
BfmI CTRYAG 4 cut(s) 1661, 1673, 1833, 2067
BfoI RGCGCY 1 cut(s) 23
BlpI GCTNAGC 1 cut(s) 1995
Bme1390I CCNGG 8 cut(s) 88, 115, 314, 1360, 1458, 1610, 1910, 2006
Bme18I GGWCC 4 cut(s) 69, 235, 310, 778
BmeT110I CYCGRG 2 cut(s) 710, 1326
BmgT120I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1636
BmiI GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
BmrFI CCNGG 8 cut(s) 88, 115, 314, 1360, 1458, 1610, 1910, 2006
BmsI GCATC 4 cut(s) 696, 1483, 1885, 1972
BpiI GAAGAC 3 cut(s) 263, 795, 921
Bpu10I CCTNAGC 4 cut(s) 1068, 1074, 1584, 1884
Bpu1102I GCTNAGC 1 cut(s) 1995
BpuMI CCSGG 1 cut(s) 1360
BsaAI YACGTR 1 cut(s) 784
BsaHI GRCGYC 2 cut(s) 20, 110
BsaI GGTCTC 1 cut(s) 1728
BsaJI CCNNGG 9 cut(s) 65, 87, 155, 538, 1257, 1456, 1908, 2004, 2076
BsaWI WCCGGW 3 cut(s) 375, 676, 1142
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 482, 512, 542, 572, 1825, 1855
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 120, 1272
Bse118I RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1719
Bse1I ACTGG 3 cut(s) 295, 500, 1241
Bse3DI GCAATG 2 cut(s) 1788, 2043
BseBI CCWGG 7 cut(s) 88, 115, 314, 1458, 1610, 1910, 2006
BseDI CCNNGG 9 cut(s) 65, 87, 155, 538, 1257, 1456, 1908, 2004, 2076
BseGI GGATG 1 cut(s) 1579
BseLI CCNNNNNNNGG 3 cut(s) 87, 120, 1272
BseMI GCAATG 2 cut(s) 1788, 2043
BseNI ACTGG 3 cut(s) 295, 500, 1241
BseRI GAGGAG 2 cut(s) 1056, 1833
BseSI GKGCMC 2 cut(s) 468, 1465
BseX3I CGGCCG 2 cut(s) 158, 275
BseYI CCCAGC 1 cut(s) 1487
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 6 cut(s) 157, 412, 509, 540, 638, 1259
Bsh1285I CGRYCG 3 cut(s) 161, 278, 339
BshFI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1637
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 3 cut(s) 161, 278, 339
BsiHKAI GWGCWC 1 cut(s) 1465
BsiHKCI CYCGRG 2 cut(s) 710, 1326
BslFI GGGAC 3 cut(s) 55, 104, 677
BslI CCNNNNNNNGG 3 cut(s) 87, 120, 1272
BsmAI GTCTC 8 cut(s) 627, 750, 1021, 1104, 1131, 1315, 1728, 1929
BsmBI CGTCTC 4 cut(s) 627, 750, 1021, 1929
BsmFI GGGAC 3 cut(s) 55, 104, 677
BsmI GAATGC 1 cut(s) 1786
BsnI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1637
Bso31I GGTCTC 1 cut(s) 1728
BsoBI CYCGRG 2 cut(s) 710, 1326
Bsp120I GGGCCC 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 468, 1465
Bsp1407I TGTACA 1 cut(s) 1818
Bsp1720I GCTNAGC 1 cut(s) 1995
Bsp19I CCATGG 1 cut(s) 2076
BspANI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1637
BspFNI CGCG 6 cut(s) 157, 412, 509, 540, 638, 1259
BspLI GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
BspMAI CTGCAG 3 cut(s) 1665, 1677, 2071
BspPI GGATC 5 cut(s) 837, 1019, 1026, 1289, 1310
BspQI GCTCTTC 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 1728
BsrBI CCGCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 1788, 2043
BsrFI RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1719
BsrGI TGTACA 1 cut(s) 1818
BsrI ACTGG 3 cut(s) 295, 500, 1241
BssAI RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1719
BssECI CCNNGG 9 cut(s) 65, 87, 155, 538, 1257, 1456, 1908, 2004, 2076
BssNAI GTATAC 1 cut(s) 2011
BssNI GRCGYC 2 cut(s) 20, 110
BssT1I CCWWGG 1 cut(s) 2076
Bst1107I GTATAC 1 cut(s) 2011
Bst2UI CCWGG 7 cut(s) 88, 115, 314, 1458, 1610, 1910, 2006
Bst4CI ACNGT 5 cut(s) 671, 812, 871, 1043, 1936
Bst6I CTCTTC 1 cut(s) 843
BstACI GRCGYC 2 cut(s) 20, 110
BstAPI GCANNNNNTGC 1 cut(s) 1672
BstAUI TGTACA 1 cut(s) 1818
BstBAI YACGTR 1 cut(s) 784
BstC8I GCNNGC 9 cut(s) 108, 231, 385, 408, 572, 756, 1374, 1626, 2001
BstDSI CCRYGG 4 cut(s) 155, 538, 1257, 2076
BstEII GGTNACC 1 cut(s) 1261
BstF5I GGATG 1 cut(s) 1579
BstFNI CGCG 6 cut(s) 157, 412, 509, 540, 638, 1259
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 5 cut(s) 22, 412, 511, 826, 973
BstMAI GTCTC 8 cut(s) 627, 750, 1021, 1104, 1131, 1315, 1728, 1929
BstMCI CGRYCG 3 cut(s) 161, 278, 339
BstNI CCWGG 7 cut(s) 88, 115, 314, 1458, 1610, 1910, 2006
BstNSI RCATGY 3 cut(s) 1474, 1624, 2003
BstPI GGTNACC 1 cut(s) 1261
BstSCI CCNGG 8 cut(s) 86, 113, 312, 1358, 1456, 1608, 1908, 2004
BstSFI CTRYAG 4 cut(s) 1661, 1673, 1833, 2067
BstSLI GKGCMC 2 cut(s) 468, 1465
BstUI CGCG 6 cut(s) 157, 412, 509, 540, 638, 1259
BstV2I GAAGAC 3 cut(s) 263, 795, 921
BstZ17I GTATAC 1 cut(s) 2011
BstZI CGGCCG 2 cut(s) 158, 275
BsuRI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1637
BtgI CCRYGG 4 cut(s) 155, 538, 1257, 2076
BtsCI GGATG 1 cut(s) 1579
BtsIMutI CAGTG 1 cut(s) 1179
Cac8I GCNNGC 9 cut(s) 108, 231, 385, 408, 572, 756, 1374, 1626, 2001
CaiI CAGNNNCTG 1 cut(s) 1955
CciNI GCGGCCGC 1 cut(s) 158
CfoI GCGC 5 cut(s) 22, 412, 511, 826, 973
Cfr10I RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1719
Cfr13I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1636
Cfr42I CCGCGG 3 cut(s) 158, 541, 1260
CseI GACGC 3 cut(s) 99, 268, 644
CsiI ACCWGGT 1 cut(s) 1608
Csp6I GTAC 4 cut(s) 1088, 1115, 1819, 2050
CviAII CATG 6 cut(s) 1471, 1621, 1991, 2000, 2059, 2077
CviQI GTAC 4 cut(s) 1088, 1115, 1819, 2050
DinI GGCGCC 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 151
EaeI YGGCCR 3 cut(s) 142, 158, 275
EagI CGGCCG 2 cut(s) 158, 275
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
EciI GGCGGA 2 cut(s) 544, 645
EclXI CGGCCG 2 cut(s) 158, 275
Eco130I CCWWGG 1 cut(s) 2076
Eco24I GRGCYC 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 1728
Eco32I GATATC 1 cut(s) 1049
Eco47I GGWCC 4 cut(s) 69, 235, 310, 778
Eco52I CGGCCG 2 cut(s) 158, 275
Eco57I CTGAAG 1 cut(s) 458
Eco72I CACGTG 1 cut(s) 784
Eco88I CYCGRG 2 cut(s) 710, 1326
Eco91I GGTNACC 1 cut(s) 1261
EcoO109I RGGNCCY 4 cut(s) 69, 310, 464, 465
EcoO65I GGTNACC 1 cut(s) 1261
EcoRII CCWGG 7 cut(s) 86, 113, 312, 1456, 1608, 1908, 2004
EcoRV GATATC 1 cut(s) 1049
EcoT14I CCWWGG 1 cut(s) 2076
EcoT22I ATGCAT 1 cut(s) 1476
EcoT38I GRGCYC 1 cut(s) 468
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 2076
Esp3I CGTCTC 4 cut(s) 627, 750, 1021, 1929
FaeI CATG 6 cut(s) 1474, 1624, 1994, 2003, 2062, 2080
FaqI GGGAC 3 cut(s) 55, 104, 677
FatI CATG 6 cut(s) 1470, 1620, 1990, 1999, 2058, 2076
FauI CCCGC 5 cut(s) 105, 111, 596, 1264, 2041
FbaI TGATCA 2 cut(s) 1183, 1384
FblI GTMKAC 1 cut(s) 2010
FokI GGATG 1 cut(s) 1566
FriOI GRGCYC 1 cut(s) 468
FspBI CTAG 3 cut(s) 581, 1716, 2027
GlaI GCGC 5 cut(s) 21, 411, 510, 825, 972
GsaI CCCAGC 1 cut(s) 1491
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1637
HgaI GACGC 3 cut(s) 99, 268, 644
HhaI GCGC 5 cut(s) 22, 412, 511, 826, 973
Hin1I GRCGYC 2 cut(s) 20, 110
Hin1II CATG 6 cut(s) 1474, 1624, 1994, 2003, 2062, 2080
Hin6I GCGC 5 cut(s) 20, 410, 509, 824, 971
HinP1I GCGC 5 cut(s) 20, 410, 509, 824, 971
HincII GTYRAC 2 cut(s) 565, 735
HindII GTYRAC 2 cut(s) 565, 735
HindIII AAGCTT 1 cut(s) 1641
HphI GGTGA 4 cut(s) 924, 988, 1472, 1910
Hpy188III TCNNGA 6 cut(s) 31, 241, 624, 712, 1235, 1352
HpyAV CCTTC 3 cut(s) 829, 972, 1536
HpyCH4III ACNGT 5 cut(s) 671, 812, 871, 1043, 1936
HpyCH4IV ACGT 3 cut(s) 183, 681, 783
HpySE526I ACGT 3 cut(s) 183, 681, 783
Hsp92I GRCGYC 2 cut(s) 20, 110
Hsp92II CATG 6 cut(s) 1474, 1624, 1994, 2003, 2062, 2080
HspAI GCGC 5 cut(s) 20, 410, 509, 824, 971
KasI GGCGCC 1 cut(s) 19
KroI GCCGGC 2 cut(s) 106, 406
KroNI GCCGGC 2 cut(s) 108, 408
Ksp22I TGATCA 2 cut(s) 1183, 1384
KspI CCGCGG 3 cut(s) 158, 541, 1260
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 5 cut(s) 4, 105, 123, 801, 1846
LweI GCATC 4 cut(s) 696, 1483, 1885, 1972
MabI ACCWGGT 1 cut(s) 1608
MaeI CTAG 3 cut(s) 581, 1716, 2027
MaeII ACGT 3 cut(s) 183, 681, 783
MaeIII GTNAC 5 cut(s) 74, 331, 806, 1261, 1901
MbiI CCGCTC 1 cut(s) 100
MboII GAAGA 7 cut(s) 268, 493, 800, 860, 921, 975, 978
MhlI GDGCHC 2 cut(s) 468, 1465
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
MmeI TCCRAC 4 cut(s) 468, 504, 573, 1704
Mph1103I ATGCAT 1 cut(s) 1476
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 2 cut(s) 106, 406
MseI TTAA 1 cut(s) 1305
MslI CAYNNNNRTG 1 cut(s) 1458
MspA1I CMGCKG 9 cut(s) 157, 436, 540, 795, 1259, 1672, 1838, 2066, 2072
MspR9I CCNGG 8 cut(s) 88, 115, 314, 1360, 1458, 1610, 1910, 2006
Mva1269I GAATGC 1 cut(s) 1786
MvaI CCWGG 7 cut(s) 88, 115, 314, 1458, 1610, 1910, 2006
MvnI CGCG 6 cut(s) 157, 412, 509, 540, 638, 1259
NaeI GCCGGC 2 cut(s) 108, 408
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 1 cut(s) 1360
NcoI CCATGG 1 cut(s) 2076
NgoMIV GCCGGC 2 cut(s) 106, 406
NlaIII CATG 6 cut(s) 1474, 1624, 1994, 2003, 2062, 2080
NlaIV GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
NmeAIII GCCGAG 3 cut(s) 90, 327, 555
NmuCI GTSAC 2 cut(s) 331, 806
NotI GCGGCCGC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 1476
NspI RCATGY 3 cut(s) 1474, 1624, 2003
OliI CACNNNNGTG 1 cut(s) 1458
PaeI GCATGC 1 cut(s) 2003
PaeR7I CTCGAG 1 cut(s) 710
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 6 cut(s) 56, 189, 455, 582, 746, 1032
PctI GAATGC 1 cut(s) 1786
PdiI GCCGGC 2 cut(s) 108, 408
PflFI GACNNNGTC 1 cut(s) 484
PleI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 784
PmlI CACGTG 1 cut(s) 784
PpsI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
Ppu21I YACGTR 1 cut(s) 784
PpuMI RGGWCCY 2 cut(s) 69, 310
Psp5II RGGWCCY 2 cut(s) 69, 310
Psp6I CCWGG 7 cut(s) 86, 113, 312, 1456, 1608, 1908, 2004
PspCI CACGTG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 1261
PspFI CCCAGC 1 cut(s) 1487
PspGI CCWGG 7 cut(s) 86, 113, 312, 1456, 1608, 1908, 2004
PspN4I GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
PspOMI GGGCCC 1 cut(s) 464
PspPI GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1636
PspPPI RGGWCCY 2 cut(s) 69, 310
PstI CTGCAG 3 cut(s) 1665, 1677, 2071
PstNI CAGNNNCTG 1 cut(s) 1955
PsyI GACNNNGTC 1 cut(s) 484
PvuII CAGCTG 3 cut(s) 1838, 2066, 2072
RsaI GTAC 4 cut(s) 1089, 1116, 1820, 2051
RsaNI GTAC 4 cut(s) 1088, 1115, 1819, 2050
RseI CAYNNNNRTG 1 cut(s) 1458
SacII CCGCGG 3 cut(s) 158, 541, 1260
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 1 cut(s) 1305
Sau96I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1636
SchI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
ScrFI CCNGG 8 cut(s) 88, 115, 314, 1360, 1458, 1610, 1910, 2006
SduI GDGCHC 2 cut(s) 468, 1465
SexAI ACCWGGT 1 cut(s) 1608
SfaNI GCATC 4 cut(s) 696, 1483, 1885, 1972
SfcI CTRYAG 4 cut(s) 1661, 1673, 1833, 2067
SfoI GGCGCC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 710
Sfr303I CCGCGG 3 cut(s) 158, 541, 1260
SgrAI CRCCGGYG 3 cut(s) 10, 106, 973
SgrBI CCGCGG 3 cut(s) 158, 541, 1260
SinI GGWCC 4 cut(s) 69, 235, 310, 778
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 1458
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SphI GCATGC 1 cut(s) 2003
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 3 cut(s) 581, 1716, 2027
StyD4I CCNGG 8 cut(s) 86, 113, 312, 1358, 1456, 1608, 1908, 2004
StyI CCWWGG 1 cut(s) 2076
TaaI ACNGT 5 cut(s) 671, 812, 871, 1043, 1936
TaiI ACGT 3 cut(s) 186, 684, 786
TaqI TCGA 9 cut(s) 199, 242, 363, 458, 471, 623, 711, 1590, 1946
TaqII GACCGA 1 cut(s) 353
TatI WGTACW 1 cut(s) 1818
Tru1I TTAA 1 cut(s) 1305
Tru9I TTAA 1 cut(s) 1305
TscAI CASTG 1 cut(s) 1179
TseFI GTSAC 2 cut(s) 331, 806
Tsp45I GTSAC 2 cut(s) 331, 806
TspDTI ATGAA 2 cut(s) 1904, 1986
TspGWI ACGGA 3 cut(s) 319, 550, 738
TspRI CASTG 1 cut(s) 1179
Tth111I GACNNNGTC 1 cut(s) 484
VneI GTGCAC 1 cut(s) 1461
VpaK11BI GGWCC 4 cut(s) 69, 235, 310, 778
XapI RAATTY 2 cut(s) 760, 1146
XceI RCATGY 3 cut(s) 1474, 1624, 2003
XhoI CTCGAG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 2010
XspI CTAG 3 cut(s) 581, 1716, 2027
Zsp2I ATGCAT 1 cut(s) 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.