Rh4DG334400

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011786
Physical Location & Seq
Forward (+)
55751603 .. 55753979
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG334400.1

Sequence Viewer

Length: 2142 bp
ATGGAGCCACCGGCGGCGGCGCCGCCACTCCAGACTCACTACGCCGACTCGCTGGACTCGTCGCCGAGGTCCCGTAACACCGACTCCTGGGACGACCCGCTCCCGCCGGCGTCCAGGCTCCGGCTGATGTGCAGCTACGGCGGCCACATCGTGCCGCGGCCGCACGACAAGTCGTTGTGCTACGTCGGCGGCGACACTCGAATCGTCGTCGTGGACAGGAACACTTCGCTTGCGGACCTCTCGAATCGTCTGGCGAAGACGCTTTTGAATAACCGGCCGTTCACGCTGAAATACCAGTTGCCGAGCGAGGACCTGGACTCGTTGATCTCCGTGACGACCGACGAGGATTTGGATAATATGATCGACGAGTTTGACCGGACGGCGAGCCACAATTCGTCGGCGAAGCCGGCGCGGATTCGGCTGTTTCTGTTTCCGCTGAAGCCGGATTCGTCGCAGTCGATGGGGCCCCTCGATATGCCGACCAAGTCGGAGGACTGGTTCTTCAACGCGCTCAACGGCTCGTCGGAGATACTCAACCGCGGCTTCTCCGACTCCGCCTCCGTCAACTGCTTGCTCGGCCTAGACGACGACGGCGGCGGCGGCGGGGCGAACAATTCGGATTCGAGAGGCGGAGACGCGGAGGCTTCTGCGGCGGCGAATTGCAAGAGCGGTAAACCGGACGTCCACTCTGTCCCCGATTCGCCGATGCTCGAGAACTCGTCGTCGTTTGGGTCAACATCGTCGTCTCCGTCGCTGGCGAATTTGCCTCCGATTCGGGTCCACGTGGAAGACAGCGGTGGGAGCAGTCACGGTGGCAATAGAGCGCAGGATCAGAAGGTTGGGATTGAAGAGCAGTTTGCTCAGATGACGGTGGGTTTGGTTGGGCAGCAGCAGAAGCAAGACGATAGCGGCGGCTTTGTGGTGCTGTCTTCACCGCCGCCGTTGCCGACGTCTATTGTGCCTTCCTCTGCGCCGGTGAGTTCTTCTTCGGCCCCTGATTACCTGAATCGGGTCGTCTCCGATGACGAACGATCCGATCATGGTGGATATCGGAAACCGCTTCCTCCTCAGCCTCAGCAACAGAGTCTACCGCCTCAGTCTCAGCCCAAATCAACAGGAATTTTTGATTTGCCGTCGCCAGATTCAGTGTCAAGTGATCATAGTTTATCAAATGCTGCCATATCTCGCCCAAAACCTGTAATTTATCAAGAACCAGTTGCTCAAATACCTGCGGTAACCCCCACAAGGTTTCCTGCAAACCTTGTTGATCCAAAAGTTAATGTCTCCGATCCAAACACTCGGGTTCAGTTGCAACAACAGGTTCAGGATTCTGGGTATGTATTGCAAGCCCAATATGATCAACAACTACAACAGCAGCAGCAACTACTACAACAACAACAACTACAACAACAGCAACAATTTATACACCCTGGTGCACATTACATGCATCATCACCCTGCTGGGTCTGTGCCAATTCAGGCATACTACCCTGTATATCCTTCTCAGCAGCAGCAGCACCAGCAGCAGCAGCACCAGCAGCAGCAACACCAGCAGCAACAGCAACACCACCATCATCCTCAGCTCGATCAGCAGCAGCAATACCAGGTTTATTACATGCCTGCAAGACAGGCCCAACCTTACAGCAACTTGCCCCTGCAGCAACCTTTGCAGTCCAACATTAGTGAAGCTGCTGCTGCTACTATTGCTTCTAGCCGGTCTCAACCGCCTCCCAATCCAATGCTCTCTCCTACCGCAGCTTACAGCCAGATAAGGAATGCCCCCATTGCCAAACCCGAAATTGCTGCTGGTGTGTACAGGACAACAACTACAGCTGCTCCTCCATCTCTGGTTCAAGTTCCCCCTACTCAGCATCAGCATCAGCCTCAGCAACAATATGTTGGTTACACACAGGTTCATCACCCGTCTCAATCAACTGTTCCTACTTCGACAGGTGCTGCTAATTATGCCTATGAATATGCCGATGCTTCTCATGCTCAGCATGCCCAGGTATACTATACTCAGCCTCTAGCACCCACAATGACTTCTCAGTACCAAACCATGACAGCCGCAGCTGCCATGGGACTGCCGGAAGTTTCAACTCAGCTTCCTAATGACAACATCAAGCAGCAGCAGCAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

713

Amino Acids

77.79

Weight (kDa)

5.73

Isoelectric Point (pI)

71.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 55 - 145 2e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01190
fragaria_vesca FvH4_4g26650 FvH4_4g26650 FvH4_4g26650
malus_domestica MD13G1034900.v1.1 MD16G1036800.v1.1
prunus_persica Prupe.1G317300_v2.0.a1
pyrus_communis pycom13g03050 pycom16g03170
rosa_chinensis RchiOBHm_Chr4g0434361
rosa_laevigata RLG00000006680
rosa_multiflora Rmu_sc0016328.1_g000003
rosa_roxburghii Rroxscaffold_5G00375720
rosa_rugosa Rorug04G0276300
rosa_samantha Rh4AG331400 Rh4BG339300 Rh4CG354400 Rh4DG334400
rosa_wichuraiana Rw4G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 684, 953
Acc36I ACCTGC 1 cut(s) 1237
AccB1I GGYRCC 1 cut(s) 19
AccBSI CCGCTC 2 cut(s) 100, 669
AccI GTMKAC 2 cut(s) 1087, 2010
AccII CGCG 5 cut(s) 157, 412, 509, 540, 638
AclWI GGATC 4 cut(s) 837, 1026, 1262, 1283
AcoI YGGCCR 3 cut(s) 142, 158, 275
AcsI RAATTY 2 cut(s) 760, 1119
AcuI CTGAAG 1 cut(s) 458
AcvI CACGTG 1 cut(s) 784
AcyI GRCGYC 4 cut(s) 20, 110, 681, 950
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 2 cut(s) 1814, 2051
AfiI CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
AgsI TTSAA 5 cut(s) 268, 505, 848, 1853, 2097
AjnI CCWGG 6 cut(s) 86, 113, 312, 1429, 1602, 2004
AleI CACNNNNGTG 1 cut(s) 1431
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
AluBI AGCT 7 cut(s) 135, 1582, 1688, 1757, 1832, 2072, 2104
AluI AGCT 7 cut(s) 135, 1582, 1688, 1757, 1832, 2072, 2104
Alw21I GWGCWC 1 cut(s) 1438
Alw26I GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1722, 1929
Alw44I GTGCAC 1 cut(s) 1434
AlwI GGATC 4 cut(s) 837, 1026, 1262, 1283
AlwNI CAGNNNCTG 1 cut(s) 1955
Ama87I CYCGRG 2 cut(s) 710, 1299
AoxI GGCC 7 cut(s) 142, 158, 275, 464, 577, 990, 1629
ApaI GGGCCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 1434
ApoI RAATTY 2 cut(s) 760, 1119
ArsI GACNNNNNNTTYG 2 cut(s) 859, 891
AspLEI GCGC 5 cut(s) 22, 412, 511, 826, 973
AspS9I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1630
AsuHPI GGTGA 4 cut(s) 924, 988, 1445, 1910
AvaI CYCGRG 2 cut(s) 710, 1299
AvaII GGWCC 4 cut(s) 69, 235, 310, 778
BaeGI GKGCMC 2 cut(s) 468, 1438
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 468
BbrPI CACGTG 1 cut(s) 784
BbsI GAAGAC 3 cut(s) 263, 795, 921
Bbv12I GWGCWC 1 cut(s) 1438
BbvCI CCTCAGC 4 cut(s) 1068, 1074, 1578, 1884
BccI CCATC 3 cut(s) 454, 1578, 1849
BceAI ACGGC 7 cut(s) 154, 262, 396, 532, 607, 925, 1117
BcgI CGANNNNNNTGC 2 cut(s) 1784, 1818
BciT130I CCWGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
BclI TGATCA 2 cut(s) 1156, 1357
BcoDI GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1722, 1929
BfaI CTAG 3 cut(s) 581, 1710, 2027
BfmI CTRYAG 2 cut(s) 1655, 1827
BfoI RGCGCY 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 1237
BlpI GCTNAGC 1 cut(s) 1995
Bme1390I CCNGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
Bme18I GGWCC 4 cut(s) 69, 235, 310, 778
BmeT110I CYCGRG 2 cut(s) 710, 1299
BmgT120I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1630
BmiI GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
BmrFI CCNGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
BmsI GCATC 5 cut(s) 696, 1456, 1879, 1885, 1972
BpiI GAAGAC 3 cut(s) 263, 795, 921
BpmI CTGGAG 1 cut(s) 14
Bpu10I CCTNAGC 4 cut(s) 1068, 1074, 1578, 1884
Bpu1102I GCTNAGC 1 cut(s) 1995
BsaAI YACGTR 1 cut(s) 784
BsaHI GRCGYC 4 cut(s) 20, 110, 681, 950
BsaI GGTCTC 1 cut(s) 1722
BsaJI CCNNGG 7 cut(s) 65, 87, 155, 538, 1429, 2004, 2076
BsaWI WCCGGW 2 cut(s) 375, 676
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 482, 512, 542, 572, 1819, 1849
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
Bse118I RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1713
Bse1I ACTGG 3 cut(s) 295, 500, 1214
Bse3DI GCAATG 1 cut(s) 1782
BseBI CCWGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
BseDI CCNNGG 7 cut(s) 65, 87, 155, 538, 1429, 2004, 2076
BseGI GGATG 1 cut(s) 1573
BseLI CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
BseMI GCAATG 1 cut(s) 1782
BseNI ACTGG 3 cut(s) 295, 500, 1214
BseRI GAGGAG 2 cut(s) 1056, 1827
BseSI GKGCMC 2 cut(s) 468, 1438
BseX3I CGGCCG 2 cut(s) 158, 275
BseYI CCCAGC 1 cut(s) 1460
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 5 cut(s) 157, 412, 509, 540, 638
Bsh1285I CGRYCG 3 cut(s) 161, 278, 339
BshFI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1631
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 3 cut(s) 161, 278, 339
BsiHKAI GWGCWC 1 cut(s) 1438
BsiHKCI CYCGRG 2 cut(s) 710, 1299
BslFI GGGAC 4 cut(s) 55, 104, 677, 2094
BslI CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
BsmAI GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1722, 1929
BsmBI CGTCTC 4 cut(s) 627, 750, 1021, 1929
BsmFI GGGAC 4 cut(s) 55, 104, 677, 2094
BsmI GAATGC 1 cut(s) 1780
BsnI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1631
Bso31I GGTCTC 1 cut(s) 1722
BsoBI CYCGRG 2 cut(s) 710, 1299
Bsp120I GGGCCC 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 468, 1438
Bsp1407I TGTACA 1 cut(s) 1812
Bsp1720I GCTNAGC 1 cut(s) 1995
Bsp19I CCATGG 1 cut(s) 2076
BspANI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1631
BspFNI CGCG 5 cut(s) 157, 412, 509, 540, 638
BspLI GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
BspMAI CTGCAG 1 cut(s) 1659
BspMI ACCTGC 1 cut(s) 1237
BspPI GGATC 4 cut(s) 837, 1026, 1262, 1283
BspQI GCTCTTC 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 1722
BsrBI CCGCTC 2 cut(s) 100, 669
BsrDI GCAATG 1 cut(s) 1782
BsrFI RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1713
BsrGI TGTACA 1 cut(s) 1812
BsrI ACTGG 3 cut(s) 295, 500, 1214
BssAI RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1713
BssECI CCNNGG 7 cut(s) 65, 87, 155, 538, 1429, 2004, 2076
BssNAI GTATAC 1 cut(s) 2011
BssNI GRCGYC 4 cut(s) 20, 110, 681, 950
BssT1I CCWWGG 1 cut(s) 2076
Bst1107I GTATAC 1 cut(s) 2011
Bst2UI CCWGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
Bst4CI ACNGT 3 cut(s) 812, 871, 1936
Bst6I CTCTTC 1 cut(s) 843
BstACI GRCGYC 4 cut(s) 20, 110, 681, 950
BstAPI GCANNNNNTGC 1 cut(s) 1666
BstAUI TGTACA 1 cut(s) 1812
BstBAI YACGTR 1 cut(s) 784
BstC8I GCNNGC 9 cut(s) 108, 231, 385, 408, 572, 756, 1347, 1620, 2001
BstDSI CCRYGG 3 cut(s) 155, 538, 2076
BstEII GGTNACC 1 cut(s) 1234
BstF5I GGATG 1 cut(s) 1573
BstFNI CGCG 5 cut(s) 157, 412, 509, 540, 638
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 5 cut(s) 22, 412, 511, 826, 973
BstMAI GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1722, 1929
BstMCI CGRYCG 3 cut(s) 161, 278, 339
BstNI CCWGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
BstNSI RCATGY 3 cut(s) 1447, 1618, 2003
BstPI GGTNACC 1 cut(s) 1234
BstSCI CCNGG 6 cut(s) 86, 113, 312, 1429, 1602, 2004
BstSFI CTRYAG 2 cut(s) 1655, 1827
BstSLI GKGCMC 2 cut(s) 468, 1438
BstUI CGCG 5 cut(s) 157, 412, 509, 540, 638
BstV2I GAAGAC 3 cut(s) 263, 795, 921
BstZ17I GTATAC 1 cut(s) 2011
BstZI CGGCCG 2 cut(s) 158, 275
BsuRI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1631
BtgI CCRYGG 3 cut(s) 155, 538, 2076
BtsCI GGATG 1 cut(s) 1573
BtsIMutI CAGTG 1 cut(s) 1152
BveI ACCTGC 1 cut(s) 1237
Cac8I GCNNGC 9 cut(s) 108, 231, 385, 408, 572, 756, 1347, 1620, 2001
CaiI CAGNNNCTG 1 cut(s) 1955
CciNI GCGGCCGC 1 cut(s) 158
CfoI GCGC 5 cut(s) 22, 412, 511, 826, 973
Cfr10I RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1713
Cfr13I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1630
Cfr42I CCGCGG 2 cut(s) 158, 541
CseI GACGC 3 cut(s) 99, 268, 644
CsiI ACCWGGT 1 cut(s) 1602
Csp6I GTAC 2 cut(s) 1813, 2050
CviAII CATG 7 cut(s) 1040, 1444, 1615, 1991, 2000, 2059, 2077
CviQI GTAC 2 cut(s) 1813, 2050
DinI GGCGCC 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 151
EaeI YGGCCR 3 cut(s) 142, 158, 275
EagI CGGCCG 2 cut(s) 158, 275
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
EciI GGCGGA 2 cut(s) 544, 645
EclXI CGGCCG 2 cut(s) 158, 275
Eco130I CCWWGG 1 cut(s) 2076
Eco24I GRGCYC 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 1722
Eco32I GATATC 1 cut(s) 1049
Eco47I GGWCC 4 cut(s) 69, 235, 310, 778
Eco52I CGGCCG 2 cut(s) 158, 275
Eco57I CTGAAG 1 cut(s) 458
Eco72I CACGTG 1 cut(s) 784
Eco88I CYCGRG 2 cut(s) 710, 1299
Eco91I GGTNACC 1 cut(s) 1234
EcoO109I RGGNCCY 4 cut(s) 69, 310, 464, 465
EcoO65I GGTNACC 1 cut(s) 1234
EcoRII CCWGG 6 cut(s) 86, 113, 312, 1429, 1602, 2004
EcoRV GATATC 1 cut(s) 1049
EcoT14I CCWWGG 1 cut(s) 2076
EcoT22I ATGCAT 1 cut(s) 1449
EcoT38I GRGCYC 1 cut(s) 468
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 2076
Esp3I CGTCTC 4 cut(s) 627, 750, 1021, 1929
FaeI CATG 7 cut(s) 1043, 1447, 1618, 1994, 2003, 2062, 2080
FaqI GGGAC 4 cut(s) 55, 104, 677, 2094
FatI CATG 7 cut(s) 1039, 1443, 1614, 1990, 1999, 2058, 2076
FauI CCCGC 3 cut(s) 105, 111, 596
FbaI TGATCA 2 cut(s) 1156, 1357
FblI GTMKAC 2 cut(s) 1087, 2010
FokI GGATG 1 cut(s) 1560
FriOI GRGCYC 1 cut(s) 468
FspBI CTAG 3 cut(s) 581, 1710, 2027
GlaI GCGC 5 cut(s) 21, 411, 510, 825, 972
GsaI CCCAGC 1 cut(s) 1464
GsuI CTGGAG 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1631
HgaI GACGC 3 cut(s) 99, 268, 644
HhaI GCGC 5 cut(s) 22, 412, 511, 826, 973
Hin1I GRCGYC 4 cut(s) 20, 110, 681, 950
Hin1II CATG 7 cut(s) 1043, 1447, 1618, 1994, 2003, 2062, 2080
Hin6I GCGC 5 cut(s) 20, 410, 509, 824, 971
HinP1I GCGC 5 cut(s) 20, 410, 509, 824, 971
HincII GTYRAC 2 cut(s) 565, 735
HindII GTYRAC 2 cut(s) 565, 735
HphI GGTGA 4 cut(s) 924, 988, 1445, 1910
Hpy188III TCNNGA 6 cut(s) 31, 241, 624, 712, 1208, 1325
HpyAV CCTTC 3 cut(s) 829, 972, 1509
HpyCH4III ACNGT 3 cut(s) 812, 871, 1936
HpyCH4IV ACGT 4 cut(s) 183, 681, 783, 950
HpySE526I ACGT 4 cut(s) 183, 681, 783, 950
Hsp92I GRCGYC 4 cut(s) 20, 110, 681, 950
Hsp92II CATG 7 cut(s) 1043, 1447, 1618, 1994, 2003, 2062, 2080
HspAI GCGC 5 cut(s) 20, 410, 509, 824, 971
KasI GGCGCC 1 cut(s) 19
KroI GCCGGC 2 cut(s) 106, 406
KroNI GCCGGC 2 cut(s) 108, 408
Ksp22I TGATCA 2 cut(s) 1156, 1357
KspI CCGCGG 2 cut(s) 158, 541
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 5 cut(s) 4, 105, 123, 801, 1840
LweI GCATC 5 cut(s) 696, 1456, 1879, 1885, 1972
MabI ACCWGGT 1 cut(s) 1602
MaeI CTAG 3 cut(s) 581, 1710, 2027
MaeII ACGT 4 cut(s) 183, 681, 783, 950
MaeIII GTNAC 5 cut(s) 74, 331, 806, 1234, 1901
MbiI CCGCTC 2 cut(s) 100, 669
MboII GAAGA 7 cut(s) 268, 493, 800, 860, 921, 975, 978
MhlI GDGCHC 2 cut(s) 468, 1438
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 7 cut(s) 28, 41, 50, 77, 311, 545, 1093
MmeI TCCRAC 4 cut(s) 468, 504, 573, 1698
Mph1103I ATGCAT 1 cut(s) 1449
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 2 cut(s) 106, 406
MseI TTAA 1 cut(s) 1278
MslI CAYNNNNRTG 1 cut(s) 1431
MspA1I CMGCKG 6 cut(s) 157, 436, 540, 795, 1832, 2072
MspR9I CCNGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
Mva1269I GAATGC 1 cut(s) 1780
MvaI CCWGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
MvnI CGCG 5 cut(s) 157, 412, 509, 540, 638
NaeI GCCGGC 2 cut(s) 108, 408
NarI GGCGCC 1 cut(s) 20
NcoI CCATGG 1 cut(s) 2076
NgoMIV GCCGGC 2 cut(s) 106, 406
NlaIII CATG 7 cut(s) 1043, 1447, 1618, 1994, 2003, 2062, 2080
NlaIV GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
NmeAIII GCCGAG 3 cut(s) 90, 327, 555
NmuCI GTSAC 2 cut(s) 331, 806
NotI GCGGCCGC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 1449
NspI RCATGY 3 cut(s) 1447, 1618, 2003
OliI CACNNNNGTG 1 cut(s) 1431
PaeI GCATGC 1 cut(s) 2003
PaeR7I CTCGAG 1 cut(s) 710
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 6 cut(s) 56, 189, 455, 582, 746, 1032
PctI GAATGC 1 cut(s) 1780
PdiI GCCGGC 2 cut(s) 108, 408
PflFI GACNNNGTC 1 cut(s) 484
PleI GAGTC 7 cut(s) 28, 41, 50, 77, 311, 545, 1092
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 784
PmlI CACGTG 1 cut(s) 784
PpsI GAGTC 7 cut(s) 28, 41, 50, 77, 311, 545, 1092
Ppu21I YACGTR 1 cut(s) 784
PpuMI RGGWCCY 2 cut(s) 69, 310
Psp5II RGGWCCY 2 cut(s) 69, 310
Psp6I CCWGG 6 cut(s) 86, 113, 312, 1429, 1602, 2004
PspCI CACGTG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 1234
PspFI CCCAGC 1 cut(s) 1460
PspGI CCWGG 6 cut(s) 86, 113, 312, 1429, 1602, 2004
PspN4I GGNNCC 9 cut(s) 6, 21, 71, 119, 465, 466, 467, 779, 993
PspOMI GGGCCC 1 cut(s) 464
PspPI GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1630
PspPPI RGGWCCY 2 cut(s) 69, 310
PstI CTGCAG 1 cut(s) 1659
PstNI CAGNNNCTG 1 cut(s) 1955
PsyI GACNNNGTC 1 cut(s) 484
PvuII CAGCTG 2 cut(s) 1832, 2072
RsaI GTAC 2 cut(s) 1814, 2051
RsaNI GTAC 2 cut(s) 1813, 2050
RseI CAYNNNNRTG 1 cut(s) 1431
SacII CCGCGG 2 cut(s) 158, 541
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 1 cut(s) 1278
Sau96I GGNCC 8 cut(s) 69, 235, 310, 464, 465, 778, 991, 1630
SchI GAGTC 7 cut(s) 28, 41, 50, 77, 311, 545, 1093
ScrFI CCNGG 6 cut(s) 88, 115, 314, 1431, 1604, 2006
SduI GDGCHC 2 cut(s) 468, 1438
SexAI ACCWGGT 1 cut(s) 1602
SfaNI GCATC 5 cut(s) 696, 1456, 1879, 1885, 1972
SfcI CTRYAG 2 cut(s) 1655, 1827
SfoI GGCGCC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 710
Sfr303I CCGCGG 2 cut(s) 158, 541
SgrAI CRCCGGYG 3 cut(s) 10, 106, 973
SgrBI CCGCGG 2 cut(s) 158, 541
SinI GGWCC 4 cut(s) 69, 235, 310, 778
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 1431
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SphI GCATGC 1 cut(s) 2003
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 3 cut(s) 581, 1710, 2027
StyD4I CCNGG 6 cut(s) 86, 113, 312, 1429, 1602, 2004
StyI CCWWGG 1 cut(s) 2076
TaaI ACNGT 3 cut(s) 812, 871, 1936
TaiI ACGT 4 cut(s) 186, 684, 786, 953
TaqI TCGA 9 cut(s) 199, 242, 363, 458, 471, 623, 711, 1584, 1946
TaqII GACCGA 1 cut(s) 353
TatI WGTACW 1 cut(s) 1812
Tru1I TTAA 1 cut(s) 1278
Tru9I TTAA 1 cut(s) 1278
TscAI CASTG 1 cut(s) 1152
TseFI GTSAC 2 cut(s) 331, 806
Tsp45I GTSAC 2 cut(s) 331, 806
TspDTI ATGAA 2 cut(s) 1904, 1986
TspGWI ACGGA 3 cut(s) 319, 550, 738
TspRI CASTG 1 cut(s) 1152
Tth111I GACNNNGTC 1 cut(s) 484
VneI GTGCAC 1 cut(s) 1434
VpaK11BI GGWCC 4 cut(s) 69, 235, 310, 778
XapI RAATTY 2 cut(s) 760, 1119
XceI RCATGY 3 cut(s) 1447, 1618, 2003
XhoI CTCGAG 1 cut(s) 710
XmiI GTMKAC 2 cut(s) 1087, 2010
XspI CTAG 3 cut(s) 581, 1710, 2027
ZraI GACGTC 2 cut(s) 682, 951
Zsp2I ATGCAT 1 cut(s) 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.