Rh4BG339300

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011786
Physical Location & Seq
Forward (+)
50168721 .. 50171910
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG339300.1

Sequence Viewer

Length: 2148 bp
ATGGAGCCACCGGCGGCGGCGCCGCCACTCCAGACTCACTACGCCGACTCGCTGGACTCGTCGCCGAGGTCCCGTAACACCGACTCCTGGGACGACCCGCTCCCGCCGGCGTCCAGGCTCCGGCTGATGTGCAGCTACGGCGGCCACATCGTGCCGCGGCCCCACGACAAGTCGTTGTGCTACGTCGGCGGCGACACTCGAATCGTCGTCGTGGACAGGAACACTTCGCTTGCGGACCTCTCGAATCGTCTGGCGAAGACGCTTTTGAATAACCGGCCGTTCACGCTGAAATACCAGTTGCCGAGTGAGGACCTGGACTCGTTGATCTCCGTGACGACCGACGAGGATTTGGATAATATGATCGACGAGTTTGACCGGACGGCGAGCCACAATTCGTCGGCGAAGCCGGCGCGGATTCGGCTGTTTCTGTTTCCGCTGAAGCCGGATTCAGCGCAGTCGATGGGGCCCCTCGATATGCCGACCAAGTCGGAGGACTGGTTCCTCAGCGCGCTCAACGGCTCGTCGGCGATGCTCAACCGCGGCTTCTCCGACTCCGCCTCCGTCAACTGCTTGCTCGGCCTAGACGACGACGGCGGCGGCGGCGCGGCGAACAATTCGGATTCGAGAGGAGGAGACGCCGAGGCTTCTGCGGCGGCGAATTGCAAGAACGGGAAACCGGACGTTCACTCTGTCCCCGATTCGCCGATGCTCGAGAACTCGTCGTCGTTTGGGTCAACATCGTCGTCTCCGTCGCTGGCGAATTTGCCTCCGATTCGGGTCCACGTGGAAGACAGCGGTGGGAGCAGTCACGGTGGGAATAGAGCGCAGGATCAGAAGGTTGGGATTGAAGAGCAGTTTGCTCAGATGACGGTGGGTTTGGTTGGGCAGCAGCAGAAGCAAGACGATGGCGGCGGCTTTGTGGTGTTGTCTTCACCTCCGCCGTTGCCGACTACTATTGTGCCTTCCTCTGCGCCGGTGAGTTCTTCTTTGGCCCCTGATTACCTGAATCGGGTCGTCTCCGATGACGAACGATCCGATCACGGTGGATATCGGAAACCGCTTCCTCCTCAGCCTCAGCAACAGGGTGTACCGCCTCAGTCTCAGCCCAAATCAACCGGAATTTTTGATTTGCCGTCGCCAGATTCAGTGTCAAGTGATCATAGTTTATCAAATGCTGCCATATCTCGCCCAAAACCTGTAATTTATCAAGAACCAGTTGCTCAAATACCCGCGGTAACCCCCACAAGGTTTCCTGGAAACCTTGTTGATCCGAAAGTTAATGTCTCCGATCCAAACACTCGGGTTCAGTTGCAACAACAGGTTCAGGATTCCGGGTATGTATTGCAAGCCCAATATGATCAACAACTACAACAACAGCAACTACTACAACAACAACAACAACTACAACAACAGCAACAATTTATACACCCTGGTGCACAGTACATGCATCATCACCCTGCTGGGTCTGTGCCAATTCAGGCATACTACCCTGTATATCCTTCTCAGCAGCAGCACCAGCAGCAGCAGCACCAGCAGCAACAACACCAGCAGCAACAACACCAGCAGCAACAGCAACACCACCATCATCCTCAGCTCGATCAGCAGCAGCAATACCAGGTTTATTACATGCCCGCAAGACAGGCCCAACCTTACAGCAACTTGCCCCTGCAGCAACCGCTGCAGTCCAACATTAGTGAAGCTGCTGCTGCTACTATTGCTTCTAGCCGGTCTCAACCTCCTCCCAATCCAATGCTCTCTCCTACCGCAGCTTACAGCCAGATAAGGAATGCCCCCATTGCCAAACCCGAAATTGCTGCTGGTGTGTACAGGACAACAACTACAGCTGCTCCTCCATCTCTGGTTCAAGTTCCTCCTACTCAGCATCAGCCTCAGCAACAATATGTTGGTTACGCACAGGTTCATCACCCGTCTCAATCAACTGTTCCTACTTCGACAGGTGCTGCTAATTATGCCTATGAATATGCCGATGCTTCTCATGCTCAGCATGCCCAGGTATACTATACTCAGCCTCTAGCACCCGCAATGACTTCTCAGTACCAAACCATGACAGCCGCAGCTGCCATGGGGCTGCCGGAAGTTTCAACTCAGCTTCCTAATGACAACATCAAGCAGCAGCAGCAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

715

Amino Acids

77.85

Weight (kDa)

5.75

Isoelectric Point (pI)

69.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 55 - 145 2e-16 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01190
fragaria_vesca FvH4_4g26650 FvH4_4g26650 FvH4_4g26650
malus_domestica MD13G1034900.v1.1 MD16G1036800.v1.1
prunus_persica Prupe.1G317300_v2.0.a1
pyrus_communis pycom13g03050 pycom16g03170
rosa_chinensis RchiOBHm_Chr4g0434361
rosa_laevigata RLG00000006680
rosa_multiflora Rmu_sc0016328.1_g000003
rosa_roxburghii Rroxscaffold_5G00375720
rosa_rugosa Rorug04G0276300
rosa_samantha Rh4AG331400 Rh4BG339300 Rh4CG354400 Rh4DG334400
rosa_wichuraiana Rw4G028740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 2016
AccII CGCG 6 cut(s) 157, 412, 509, 540, 605, 1232
AclWI GGATC 4 cut(s) 837, 1026, 1262, 1283
AcoI YGGCCR 2 cut(s) 142, 275
AcsI RAATTY 2 cut(s) 760, 1119
AcuI CTGAAG 1 cut(s) 458
AcvI CACGTG 1 cut(s) 784
AcyI GRCGYC 3 cut(s) 20, 110, 636
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 4 cut(s) 1089, 1442, 1826, 2057
AfiI CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
AgsI TTSAA 4 cut(s) 268, 848, 1865, 2103
AjnI CCWGG 7 cut(s) 86, 113, 312, 1252, 1429, 1614, 2010
AleI CACNNNNGTG 1 cut(s) 1431
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
AluBI AGCT 7 cut(s) 135, 1594, 1700, 1769, 1844, 2078, 2110
AluI AGCT 7 cut(s) 135, 1594, 1700, 1769, 1844, 2078, 2110
Alw21I GWGCWC 1 cut(s) 1438
Alw26I GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1734, 1935
Alw44I GTGCAC 1 cut(s) 1434
AlwI GGATC 4 cut(s) 837, 1026, 1262, 1283
AlwNI CAGNNNCTG 1 cut(s) 1961
Ama87I CYCGRG 2 cut(s) 710, 1299
AoxI GGCC 7 cut(s) 142, 158, 275, 464, 577, 990, 1641
ApaI GGGCCC 1 cut(s) 468
ApaLI GTGCAC 1 cut(s) 1434
ApoI RAATTY 2 cut(s) 760, 1119
ArsI GACNNNNNNTTYG 2 cut(s) 859, 891
AspLEI GCGC 8 cut(s) 22, 412, 454, 509, 511, 605, 826, 973
AspS9I GGNCC 9 cut(s) 69, 159, 235, 310, 464, 465, 778, 991, 1642
AsuC2I CCSGG 1 cut(s) 1333
AsuHPI GGTGA 4 cut(s) 924, 988, 1445, 1916
AvaI CYCGRG 2 cut(s) 710, 1299
AvaII GGWCC 4 cut(s) 69, 235, 310, 778
BaeGI GKGCMC 2 cut(s) 468, 1438
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 468
BbrPI CACGTG 1 cut(s) 784
BbsI GAAGAC 3 cut(s) 263, 795, 921
Bbv12I GWGCWC 1 cut(s) 1438
BbvCI CCTCAGC 5 cut(s) 503, 1068, 1074, 1590, 1890
BccI CCATC 4 cut(s) 454, 899, 1590, 1861
BceAI ACGGC 7 cut(s) 154, 262, 396, 532, 607, 925, 1117
BcgI CGANNNNNNTGC 4 cut(s) 629, 663, 1796, 1830
BciT130I CCWGG 7 cut(s) 88, 115, 314, 1254, 1431, 1616, 2012
BclI TGATCA 2 cut(s) 1156, 1357
BcnI CCSGG 1 cut(s) 1333
BcoDI GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1734, 1935
BfaI CTAG 3 cut(s) 581, 1722, 2033
BfmI CTRYAG 3 cut(s) 1667, 1679, 1839
BfoI RGCGCY 1 cut(s) 23
BlpI GCTNAGC 1 cut(s) 2001
Bme1390I CCNGG 8 cut(s) 88, 115, 314, 1254, 1333, 1431, 1616, 2012
Bme18I GGWCC 4 cut(s) 69, 235, 310, 778
BmeT110I CYCGRG 2 cut(s) 710, 1299
BmgT120I GGNCC 9 cut(s) 69, 159, 235, 310, 464, 465, 778, 991, 1642
BmrFI CCNGG 8 cut(s) 88, 115, 314, 1254, 1333, 1431, 1616, 2012
BmsI GCATC 5 cut(s) 519, 696, 1456, 1891, 1978
BpiI GAAGAC 3 cut(s) 263, 795, 921
BpmI CTGGAG 1 cut(s) 14
Bpu10I CCTNAGC 5 cut(s) 503, 1068, 1074, 1590, 1890
Bpu1102I GCTNAGC 1 cut(s) 2001
BpuMI CCSGG 1 cut(s) 1333
BsaAI YACGTR 1 cut(s) 784
BsaHI GRCGYC 3 cut(s) 20, 110, 636
BsaI GGTCTC 1 cut(s) 1734
BsaJI CCNNGG 9 cut(s) 65, 87, 155, 538, 639, 1230, 1429, 2010, 2082
BsaWI WCCGGW 3 cut(s) 375, 676, 1115
BsaXI ACNNNNNCTCC 8 cut(s) 68, 98, 482, 512, 542, 572, 1831, 1861
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
Bse118I RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1725
Bse1I ACTGG 3 cut(s) 295, 500, 1214
Bse3DI GCAATG 2 cut(s) 1794, 2049
BseBI CCWGG 7 cut(s) 88, 115, 314, 1254, 1431, 1616, 2012
BseDI CCNNGG 9 cut(s) 65, 87, 155, 538, 639, 1230, 1429, 2010, 2082
BseGI GGATG 1 cut(s) 1585
BseLI CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
BseMI GCAATG 2 cut(s) 1794, 2049
BseNI ACTGG 3 cut(s) 295, 500, 1214
BsePI GCGCGC 1 cut(s) 507
BseRI GAGGAG 5 cut(s) 642, 645, 1056, 1728, 1839
BseSI GKGCMC 2 cut(s) 468, 1438
BseX3I CGGCCG 1 cut(s) 275
BseYI CCCAGC 1 cut(s) 1460
BsgI GTGCAG 1 cut(s) 151
Bsh1236I CGCG 6 cut(s) 157, 412, 509, 540, 605, 1232
Bsh1285I CGRYCG 2 cut(s) 278, 339
BshFI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1643
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 2 cut(s) 278, 339
BsiHKAI GWGCWC 1 cut(s) 1438
BsiHKCI CYCGRG 2 cut(s) 710, 1299
BslFI GGGAC 3 cut(s) 55, 104, 677
BslI CCNNNNNNNGG 4 cut(s) 87, 120, 1009, 1245
BsmAI GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1734, 1935
BsmBI CGTCTC 4 cut(s) 627, 750, 1021, 1935
BsmFI GGGAC 3 cut(s) 55, 104, 677
BsmI GAATGC 1 cut(s) 1792
BsnI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1643
Bso31I GGTCTC 1 cut(s) 1734
BsoBI CYCGRG 2 cut(s) 710, 1299
Bsp120I GGGCCC 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 468, 1438
Bsp1407I TGTACA 1 cut(s) 1824
Bsp1720I GCTNAGC 1 cut(s) 2001
Bsp19I CCATGG 1 cut(s) 2082
BspANI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1643
BspFNI CGCG 6 cut(s) 157, 412, 509, 540, 605, 1232
BspMAI CTGCAG 2 cut(s) 1671, 1683
BspPI GGATC 4 cut(s) 837, 1026, 1262, 1283
BspQI GCTCTTC 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 1734
BsrBI CCGCTC 1 cut(s) 100
BsrDI GCAATG 2 cut(s) 1794, 2049
BsrFI RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1725
BsrGI TGTACA 1 cut(s) 1824
BsrI ACTGG 3 cut(s) 295, 500, 1214
BssAI RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1725
BssECI CCNNGG 9 cut(s) 65, 87, 155, 538, 639, 1230, 1429, 2010, 2082
BssHII GCGCGC 1 cut(s) 507
BssNAI GTATAC 1 cut(s) 2017
BssNI GRCGYC 3 cut(s) 20, 110, 636
BssT1I CCWWGG 1 cut(s) 2082
Bst1107I GTATAC 1 cut(s) 2017
Bst2UI CCWGG 7 cut(s) 88, 115, 314, 1254, 1431, 1616, 2012
Bst4CI ACNGT 5 cut(s) 812, 871, 1043, 1440, 1942
Bst6I CTCTTC 1 cut(s) 843
BstACI GRCGYC 3 cut(s) 20, 110, 636
BstAPI GCANNNNNTGC 1 cut(s) 1678
BstAUI TGTACA 1 cut(s) 1824
BstBAI YACGTR 1 cut(s) 784
BstDSI CCRYGG 4 cut(s) 155, 538, 1230, 2082
BstEII GGTNACC 1 cut(s) 1234
BstF5I GGATG 1 cut(s) 1585
BstFNI CGCG 6 cut(s) 157, 412, 509, 540, 605, 1232
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 8 cut(s) 22, 412, 454, 509, 511, 605, 826, 973
BstMAI GTCTC 7 cut(s) 627, 750, 1021, 1104, 1288, 1734, 1935
BstMCI CGRYCG 2 cut(s) 278, 339
BstNI CCWGG 7 cut(s) 88, 115, 314, 1254, 1431, 1616, 2012
BstNSI RCATGY 3 cut(s) 1447, 1630, 2009
BstPI GGTNACC 1 cut(s) 1234
BstSCI CCNGG 8 cut(s) 86, 113, 312, 1252, 1331, 1429, 1614, 2010
BstSFI CTRYAG 3 cut(s) 1667, 1679, 1839
BstSLI GKGCMC 2 cut(s) 468, 1438
BstUI CGCG 6 cut(s) 157, 412, 509, 540, 605, 1232
BstV2I GAAGAC 3 cut(s) 263, 795, 921
BstZ17I GTATAC 1 cut(s) 2017
BstZI CGGCCG 1 cut(s) 275
BsuRI GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1643
BtgI CCRYGG 4 cut(s) 155, 538, 1230, 2082
BtgZI GCGATG 1 cut(s) 542
BtsCI GGATG 1 cut(s) 1585
BtsIMutI CAGTG 1 cut(s) 1152
CaiI CAGNNNCTG 1 cut(s) 1961
CfoI GCGC 8 cut(s) 22, 412, 454, 509, 511, 605, 826, 973
Cfr10I RCCGGY 6 cut(s) 10, 106, 273, 406, 973, 1725
Cfr13I GGNCC 9 cut(s) 69, 159, 235, 310, 464, 465, 778, 991, 1642
Cfr42I CCGCGG 3 cut(s) 158, 541, 1233
CseI GACGC 3 cut(s) 99, 268, 644
CsiI ACCWGGT 1 cut(s) 1614
Csp6I GTAC 4 cut(s) 1088, 1441, 1825, 2056
CviAII CATG 6 cut(s) 1444, 1627, 1997, 2006, 2065, 2083
CviQI GTAC 4 cut(s) 1088, 1441, 1825, 2056
DinI GGCGCC 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 151
EaeI YGGCCR 2 cut(s) 142, 275
EagI CGGCCG 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 843
EarI CTCTTC 1 cut(s) 843
EciI GGCGGA 2 cut(s) 544, 927
EclXI CGGCCG 1 cut(s) 275
Eco130I CCWWGG 1 cut(s) 2082
Eco24I GRGCYC 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 1734
Eco32I GATATC 1 cut(s) 1049
Eco47I GGWCC 4 cut(s) 69, 235, 310, 778
Eco52I CGGCCG 1 cut(s) 275
Eco57I CTGAAG 1 cut(s) 458
Eco72I CACGTG 1 cut(s) 784
Eco88I CYCGRG 2 cut(s) 710, 1299
Eco91I GGTNACC 1 cut(s) 1234
EcoO109I RGGNCCY 4 cut(s) 69, 310, 464, 465
EcoO65I GGTNACC 1 cut(s) 1234
EcoRII CCWGG 7 cut(s) 86, 113, 312, 1252, 1429, 1614, 2010
EcoRV GATATC 1 cut(s) 1049
EcoT14I CCWWGG 1 cut(s) 2082
EcoT22I ATGCAT 1 cut(s) 1449
EcoT38I GRGCYC 1 cut(s) 468
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 2082
Esp3I CGTCTC 4 cut(s) 627, 750, 1021, 1935
FaeI CATG 6 cut(s) 1447, 1630, 2000, 2009, 2068, 2086
FaqI GGGAC 3 cut(s) 55, 104, 677
FatI CATG 6 cut(s) 1443, 1626, 1996, 2005, 2064, 2082
FauI CCCGC 5 cut(s) 105, 111, 1237, 1639, 2047
FbaI TGATCA 2 cut(s) 1156, 1357
FblI GTMKAC 1 cut(s) 2016
FokI GGATG 1 cut(s) 1572
FriOI GRGCYC 1 cut(s) 468
FspBI CTAG 3 cut(s) 581, 1722, 2033
GlaI GCGC 8 cut(s) 21, 411, 453, 508, 510, 604, 825, 972
GsaI CCCAGC 1 cut(s) 1464
GsuI CTGGAG 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 7 cut(s) 144, 160, 277, 466, 579, 992, 1643
HgaI GACGC 3 cut(s) 99, 268, 644
HhaI GCGC 8 cut(s) 22, 412, 454, 509, 511, 605, 826, 973
Hin1I GRCGYC 3 cut(s) 20, 110, 636
Hin1II CATG 6 cut(s) 1447, 1630, 2000, 2009, 2068, 2086
Hin6I GCGC 8 cut(s) 20, 410, 452, 507, 509, 603, 824, 971
HinP1I GCGC 8 cut(s) 20, 410, 452, 507, 509, 603, 824, 971
HincII GTYRAC 2 cut(s) 565, 735
HindII GTYRAC 2 cut(s) 565, 735
HphI GGTGA 4 cut(s) 924, 988, 1445, 1916
Hpy188III TCNNGA 6 cut(s) 31, 241, 624, 712, 1208, 1325
HpyAV CCTTC 3 cut(s) 829, 972, 1509
HpyCH4III ACNGT 5 cut(s) 812, 871, 1043, 1440, 1942
HpyCH4IV ACGT 3 cut(s) 183, 681, 783
HpyCH4V TGCA 8 cut(s) 132, 663, 1312, 1345, 1436, 1447, 1669, 1681
HpySE526I ACGT 3 cut(s) 183, 681, 783
Hsp92I GRCGYC 3 cut(s) 20, 110, 636
Hsp92II CATG 6 cut(s) 1447, 1630, 2000, 2009, 2068, 2086
HspAI GCGC 8 cut(s) 20, 410, 452, 507, 509, 603, 824, 971
KasI GGCGCC 1 cut(s) 19
KroI GCCGGC 2 cut(s) 106, 406
KroNI GCCGGC 2 cut(s) 108, 408
Ksp22I TGATCA 2 cut(s) 1156, 1357
KspI CCGCGG 3 cut(s) 158, 541, 1233
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 5 cut(s) 4, 105, 123, 801, 1852
LweI GCATC 5 cut(s) 519, 696, 1456, 1891, 1978
MabI ACCWGGT 1 cut(s) 1614
MaeI CTAG 3 cut(s) 581, 1722, 2033
MaeII ACGT 3 cut(s) 183, 681, 783
MaeIII GTNAC 5 cut(s) 74, 331, 806, 1234, 1907
MbiI CCGCTC 1 cut(s) 100
MboII GAAGA 5 cut(s) 268, 800, 860, 921, 975
MhlI GDGCHC 2 cut(s) 468, 1438
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
MmeI TCCRAC 3 cut(s) 468, 573, 1710
Mph1103I ATGCAT 1 cut(s) 1449
MreI CGCCGGCG 1 cut(s) 106
MroNI GCCGGC 2 cut(s) 106, 406
MseI TTAA 1 cut(s) 1278
MslI CAYNNNNRTG 1 cut(s) 1431
MspA1I CMGCKG 8 cut(s) 157, 436, 540, 795, 1232, 1678, 1844, 2078
MspR9I CCNGG 8 cut(s) 88, 115, 314, 1254, 1333, 1431, 1616, 2012
Mva1269I GAATGC 1 cut(s) 1792
MvaI CCWGG 7 cut(s) 88, 115, 314, 1254, 1431, 1616, 2012
MvnI CGCG 6 cut(s) 157, 412, 509, 540, 605, 1232
NaeI GCCGGC 2 cut(s) 108, 408
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 1 cut(s) 1333
NcoI CCATGG 1 cut(s) 2082
NgoMIV GCCGGC 2 cut(s) 106, 406
NlaIII CATG 6 cut(s) 1447, 1630, 2000, 2009, 2068, 2086
NmeAIII GCCGAG 4 cut(s) 90, 327, 555, 664
NmuCI GTSAC 2 cut(s) 331, 806
NsiI ATGCAT 1 cut(s) 1449
NspI RCATGY 3 cut(s) 1447, 1630, 2009
OliI CACNNNNGTG 1 cut(s) 1431
PaeI GCATGC 1 cut(s) 2009
PaeR7I CTCGAG 1 cut(s) 710
PauI GCGCGC 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 843
PcsI WCGNNNNNNNCGW 5 cut(s) 56, 189, 582, 746, 1032
PctI GAATGC 1 cut(s) 1792
PdiI GCCGGC 2 cut(s) 108, 408
PflFI GACNNNGTC 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 1252
PleI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
PluTI GGCGCC 1 cut(s) 23
PmaCI CACGTG 1 cut(s) 784
PmlI CACGTG 1 cut(s) 784
PpsI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
Ppu21I YACGTR 1 cut(s) 784
PpuMI RGGWCCY 2 cut(s) 69, 310
Psp5II RGGWCCY 2 cut(s) 69, 310
Psp6I CCWGG 7 cut(s) 86, 113, 312, 1252, 1429, 1614, 2010
PspCI CACGTG 1 cut(s) 784
PspEI GGTNACC 1 cut(s) 1234
PspFI CCCAGC 1 cut(s) 1460
PspGI CCWGG 7 cut(s) 86, 113, 312, 1252, 1429, 1614, 2010
PspOMI GGGCCC 1 cut(s) 464
PspPI GGNCC 9 cut(s) 69, 159, 235, 310, 464, 465, 778, 991, 1642
PspPPI RGGWCCY 2 cut(s) 69, 310
PstI CTGCAG 2 cut(s) 1671, 1683
PstNI CAGNNNCTG 1 cut(s) 1961
PsyI GACNNNGTC 1 cut(s) 484
PteI GCGCGC 1 cut(s) 507
PvuII CAGCTG 2 cut(s) 1844, 2078
RsaI GTAC 4 cut(s) 1089, 1442, 1826, 2057
RsaNI GTAC 4 cut(s) 1088, 1441, 1825, 2056
RseI CAYNNNNRTG 1 cut(s) 1431
SacII CCGCGG 3 cut(s) 158, 541, 1233
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 1 cut(s) 1278
Sau96I GGNCC 9 cut(s) 69, 159, 235, 310, 464, 465, 778, 991, 1642
SchI GAGTC 6 cut(s) 28, 41, 50, 77, 311, 545
ScrFI CCNGG 8 cut(s) 88, 115, 314, 1254, 1333, 1431, 1616, 2012
SduI GDGCHC 2 cut(s) 468, 1438
SexAI ACCWGGT 1 cut(s) 1614
SfaNI GCATC 5 cut(s) 519, 696, 1456, 1891, 1978
SfcI CTRYAG 3 cut(s) 1667, 1679, 1839
SfoI GGCGCC 1 cut(s) 21
Sfr274I CTCGAG 1 cut(s) 710
Sfr303I CCGCGG 3 cut(s) 158, 541, 1233
SgrAI CRCCGGYG 3 cut(s) 10, 106, 973
SgrBI CCGCGG 3 cut(s) 158, 541, 1233
SinI GGWCC 4 cut(s) 69, 235, 310, 778
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 1431
SmlI CTYRAG 1 cut(s) 710
SmoI CTYRAG 1 cut(s) 710
SphI GCATGC 1 cut(s) 2009
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 3 cut(s) 581, 1722, 2033
StyD4I CCNGG 8 cut(s) 86, 113, 312, 1252, 1331, 1429, 1614, 2010
StyI CCWWGG 1 cut(s) 2082
TaaI ACNGT 5 cut(s) 812, 871, 1043, 1440, 1942
TaiI ACGT 3 cut(s) 186, 684, 786
TaqI TCGA 9 cut(s) 199, 242, 363, 458, 471, 623, 711, 1596, 1952
TaqII GACCGA 1 cut(s) 353
TatI WGTACW 2 cut(s) 1440, 1824
Tru1I TTAA 1 cut(s) 1278
Tru9I TTAA 1 cut(s) 1278
TscAI CASTG 1 cut(s) 1152
TseFI GTSAC 2 cut(s) 331, 806
Tsp45I GTSAC 2 cut(s) 331, 806
TspDTI ATGAA 2 cut(s) 1910, 1992
TspGWI ACGGA 3 cut(s) 319, 550, 738
TspRI CASTG 1 cut(s) 1152
Tth111I GACNNNGTC 1 cut(s) 484
VneI GTGCAC 1 cut(s) 1434
VpaK11BI GGWCC 4 cut(s) 69, 235, 310, 778
XapI RAATTY 2 cut(s) 760, 1119
XceI RCATGY 3 cut(s) 1447, 1630, 2009
XhoI CTCGAG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 2016
XspI CTAG 3 cut(s) 581, 1722, 2033
Zsp2I ATGCAT 1 cut(s) 1449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.