FvH4_4g29640

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005916
Physical Location & Seq
Forward (+)
29681641 .. 29685151
3511 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29640.t1

Sequence Viewer

Length: 1503 bp
ATGGAGCCTTGGAGTTTCGGTTCAGAAGGGAAAGGGTTTTTATTTGCTGATGAGATGGATATACCAATTGATGCATTTGCAAAAAGTACAAAAGCATTTTTAGAATGGGATAACAAAACTTGCTTTAATTTCGATAAAGAATCAGTTGAGAGCATGGAGTTCATGGATTCGGGATTGCCTGATACAATGAAGGAGCCTTTTCATGGTAACCAGGTAGTGGATATCTTGAGTGGTGTCGGCTGTAATGATTCTAGTAACAATACAGTAGCTTCTCCTACTTGTTTGATGACTTCAAGTTCATCATGGGGTGACGGAGAATCTGGCTCCAAATTTTCTAGCCCAGTCTGTGAGTCTAGTAGCCAGGATTCATCACTAATTGCTTTGAAGCTAGGGAGGTTGGCTGATAGCAAATATGGAAAGGATAGTCAACATCTGAAAGAGAGGTCAAAGAGAGCTCGCACGAAGGGTTCATACTATCAGACTATCACCTGCCAGGTTCATGGTTGTAACATGGATCTTAGCTCTTCAAAGGAGTACCATAAGAGGCATAGAGTTTGTGATGCTCACTCAAAGACTGCGATAGTTATTGTAAATGGCATTGAACAGAGGTTTTGTCAGCAATGTAGCAGGTTTCATCTGCTGGCTGAATTCGATGATGTTAAGCGTAGTTGTCGTAGACGCTTAGCTGGCCACAATCAACGCCGAAGGAAGCCTCAGCTAGATACCTTTGATTGTAAATCGCATAACTGGCTTCAGTCATATCAAGGCATTGGATATCTTGGAACTTCCTTGCCAAAGAGAACACCATTTGTCTTTTCAGATATACTTCCAGGTGGCGTTCTATATCCAGAAGAATCTAACCAGTATGGACATATTAAAACTGAAGATGAATTAACTTACAGTCAATGTGTGACACGCACATCAAACAGGCAATCAATTCCAAACTCTTTCCTCCATCTGCATGGCCTCGCAAAGCAACATGCTTCAGGAGTTCCTATATCAGGACATGAATACTCGGTTTTTGATACAGCATCAGATGTTCAGAAAGAATCTGGGGCGTCAACCTACCAGGGTGTTCTCTCTCTTCTGTCATCTCAATCACAGAGCTCAATCCATTCTGCAAGAAATCAAGTGGCTACCCCTCTGCAAATGCAGAGAACCCATCATGGTGCTGGTCAAAATAATGAATGGCCTTCCACAGTAAAAGCCTTGGAAAAATGTGGACGAAATGGATCCTTCCCATGCAGGATGAACTCCATGGGAACTGACCAGATGGATTCGGTAATGATTTCTGATTCTAGTCATGCTGTTGACTTCCAACATCGCACAGATAGGGTCCAGGGGTCAGCTTGCTTCAGTCACAAGTATCATCTTTCTCCTGAAAATGGACCAACTTTTGATTTGCTCCAATTGTCTTCACATTTTCAAAGAGTGGAGCAAGAGAGAAACTTTTTGCAAGTCAAACAGGAACAGGGGGATTTCTACTGTTTCCCGCAATCTTAA

Protein Analysis

501

Amino Acids

55.99

Weight (kDa)

6.74

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 164 - 237 8.5e-32 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 497
Acc36I ACCTGC 2 cut(s) 497, 618
AccB7I CCANNNNNTGG 1 cut(s) 217
AccI GTMKAC 1 cut(s) 676
AciI CCGC 1 cut(s) 1493
AclWI GGATC 3 cut(s) 522, 1227, 1240
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 2 cut(s) 329, 647
AcuI CTGAAG 4 cut(s) 737, 903, 969, 1339
AcyI GRCGYC 1 cut(s) 1058
AfaI GTAC 2 cut(s) 88, 536
AfiI CCNNNNNNNGG 4 cut(s) 203, 217, 1001, 1385
AgsI TTSAA 5 cut(s) 294, 385, 528, 602, 1427
AjnI CCWGG 6 cut(s) 210, 360, 492, 829, 1068, 1338
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 8 cut(s) 269, 388, 455, 522, 686, 718, 1107, 1349
AluI AGCT 8 cut(s) 269, 388, 455, 522, 686, 718, 1107, 1349
Alw21I GWGCWC 2 cut(s) 457, 1109
AlwI GGATC 3 cut(s) 522, 1227, 1240
AoxI GGCC 3 cut(s) 688, 964, 1190
ApoI RAATTY 2 cut(s) 329, 647
AspS9I GGNCC 2 cut(s) 1336, 1388
AsuHPI GGTGA 2 cut(s) 320, 478
AvaII GGWCC 2 cut(s) 1336, 1388
BalI TGGCCA 1 cut(s) 690
BamHI GGATCC 1 cut(s) 1232
BanII GRGCYC 2 cut(s) 457, 1109
BarI GAAGNNNNNNTAC 2 cut(s) 455, 487
BbsI GAAGAC 1 cut(s) 1407
Bbv12I GWGCWC 2 cut(s) 457, 1109
BbvCI CCTCAGC 1 cut(s) 714
BccI CCATC 4 cut(s) 49, 963, 1170, 1267
BciT130I CCWGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
BfaI CTAG 6 cut(s) 252, 336, 354, 389, 719, 1299
BfuAI ACCTGC 2 cut(s) 497, 618
BlpI GCTNAGC 1 cut(s) 682
Bme1390I CCNGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
Bme18I GGWCC 2 cut(s) 1336, 1388
BmgT120I GGNCC 2 cut(s) 1336, 1388
BmiI GGNNCC 5 cut(s) 6, 195, 325, 1234, 1337
BmrFI CCNGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 3 cut(s) 61, 550, 1040
BmuI ACTGGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 1407
Bpu10I CCTNAGC 1 cut(s) 714
Bpu1102I GCTNAGC 1 cut(s) 682
BpuEI CTTGAG 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 1058
BsaJI CCNNGG 5 cut(s) 8, 1069, 1209, 1257, 1339
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 217, 1001, 1385
Bse1I ACTGG 3 cut(s) 341, 752, 862
Bse3DI GCAATG 1 cut(s) 626
BseBI CCWGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
BseDI CCNNGG 5 cut(s) 8, 1069, 1209, 1257, 1339
BseGI GGATG 1 cut(s) 1254
BseLI CCNNNNNNNGG 4 cut(s) 203, 217, 1001, 1385
BseMI GCAATG 1 cut(s) 626
BseMII CTCAG 1 cut(s) 728
BseNI ACTGG 3 cut(s) 341, 752, 862
BshFI GGCC 3 cut(s) 690, 966, 1192
BsiHKAI GWGCWC 2 cut(s) 457, 1109
BslI CCNNNNNNNGG 4 cut(s) 203, 217, 1001, 1385
BsnI GGCC 3 cut(s) 690, 966, 1192
Bsp1286I GDGCHC 2 cut(s) 457, 1109
Bsp143I GATC 2 cut(s) 514, 1232
Bsp1720I GCTNAGC 1 cut(s) 682
Bsp19I CCATGG 1 cut(s) 1257
BspACI CCGC 1 cut(s) 1493
BspANI GGCC 3 cut(s) 690, 966, 1192
BspCNI CTCAG 1 cut(s) 727
BspLI GGNNCC 5 cut(s) 6, 195, 325, 1234, 1337
BspMI ACCTGC 2 cut(s) 497, 618
BspPI GGATC 3 cut(s) 522, 1227, 1240
BspQI GCTCTTC 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 626
BsrI ACTGG 3 cut(s) 341, 752, 862
BssECI CCNNGG 5 cut(s) 8, 1069, 1209, 1257, 1339
BssMI GATC 2 cut(s) 514, 1232
BssNI GRCGYC 1 cut(s) 1058
BssT1I CCWWGG 3 cut(s) 8, 1209, 1257
Bst2UI CCWGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
Bst4CI ACNGT 4 cut(s) 265, 902, 1201, 1487
Bst6I CTCTTC 2 cut(s) 529, 1089
BstACI GRCGYC 1 cut(s) 1058
BstC8I GCNNGC 4 cut(s) 457, 642, 688, 1351
BstDEI CTNAG 3 cut(s) 518, 682, 714
BstDSI CCRYGG 1 cut(s) 1257
BstEII GGTNACC 1 cut(s) 206
BstENI CCTNNNNNAGG 1 cut(s) 999
BstF5I GGATG 1 cut(s) 1254
BstKTI GATC 2 cut(s) 517, 1235
BstMBI GATC 2 cut(s) 514, 1232
BstMWI GCNNNNNNNGC 2 cut(s) 687, 748
BstNI CCWGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
BstNSI RCATGY 1 cut(s) 983
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 6 cut(s) 210, 360, 492, 829, 1068, 1338
BstV2I GAAGAC 1 cut(s) 1407
BstX2I RGATCY 2 cut(s) 514, 1232
BstXI CCANNNNNNTGG 2 cut(s) 500, 962
BstYI RGATCY 2 cut(s) 514, 1232
BsuRI GGCC 3 cut(s) 690, 966, 1192
BtgI CCRYGG 1 cut(s) 1257
BtgZI GCGATG 1 cut(s) 1307
BtsCI GGATG 1 cut(s) 1254
BveI ACCTGC 2 cut(s) 497, 618
Cac8I GCNNGC 4 cut(s) 457, 642, 688, 1351
Cfr13I GGNCC 2 cut(s) 1336, 1388
CseI GACGC 2 cut(s) 687, 1047
CsiI ACCWGGT 1 cut(s) 210
Csp6I GTAC 2 cut(s) 87, 535
CviQI GTAC 2 cut(s) 87, 535
DdeI CTNAG 3 cut(s) 518, 682, 714
DpnI GATC 2 cut(s) 516, 1234
DpnII GATC 2 cut(s) 514, 1232
EaeI YGGCCR 1 cut(s) 688
Eam1104I CTCTTC 2 cut(s) 529, 1089
EarI CTCTTC 2 cut(s) 529, 1089
Ecl136II GAGCTC 2 cut(s) 455, 1107
Eco130I CCWWGG 3 cut(s) 8, 1209, 1257
Eco24I GRGCYC 2 cut(s) 457, 1109
Eco32I GATATC 2 cut(s) 223, 776
Eco47I GGWCC 2 cut(s) 1336, 1388
Eco53kI GAGCTC 2 cut(s) 455, 1107
Eco57I CTGAAG 4 cut(s) 737, 903, 969, 1339
Eco91I GGTNACC 1 cut(s) 206
EcoICRI GAGCTC 2 cut(s) 455, 1107
EcoNI CCTNNNNNAGG 1 cut(s) 999
EcoO65I GGTNACC 1 cut(s) 206
EcoRI GAATTC 1 cut(s) 647
EcoRII CCWGG 6 cut(s) 210, 360, 492, 829, 1068, 1338
EcoRV GATATC 2 cut(s) 223, 776
EcoT14I CCWWGG 3 cut(s) 8, 1209, 1257
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 2 cut(s) 457, 1109
ErhI CCWWGG 3 cut(s) 8, 1209, 1257
FauI CCCGC 1 cut(s) 1500
FblI GTMKAC 1 cut(s) 676
FokI GGATG 1 cut(s) 1261
FriOI GRGCYC 2 cut(s) 457, 1109
FspBI CTAG 6 cut(s) 252, 336, 354, 389, 719, 1299
HaeIII GGCC 3 cut(s) 690, 966, 1192
HgaI GACGC 2 cut(s) 687, 1047
Hin1I GRCGYC 1 cut(s) 1058
HincII GTYRAC 3 cut(s) 428, 1062, 1312
HindII GTYRAC 3 cut(s) 428, 1062, 1312
HphI GGTGA 2 cut(s) 320, 478
Hpy166II GTNNAC 5 cut(s) 428, 677, 1062, 1223, 1312
Hpy188I TCNGA 7 cut(s) 25, 435, 480, 820, 1036, 1044, 1294
Hpy188III TCNNGA 6 cut(s) 171, 226, 848, 987, 1002, 1379
Hpy8I GTNNAC 5 cut(s) 428, 677, 1062, 1223, 1312
HpyAV CCTTC 6 cut(s) 20, 184, 457, 699, 1203, 1246
HpyCH4III ACNGT 4 cut(s) 265, 902, 1201, 1487
HpyCH4V TGCA 8 cut(s) 74, 80, 961, 1121, 1147, 1153, 1245, 1456
HpyF10VI GCNNNNNNNGC 2 cut(s) 687, 748
HpyF3I CTNAG 3 cut(s) 518, 682, 714
Hsp92I GRCGYC 1 cut(s) 1058
Kzo9I GATC 2 cut(s) 514, 1232
LguI GCTCTTC 1 cut(s) 529
LmnI GCTCC 5 cut(s) 4, 193, 329, 1410, 1435
LweI GCATC 3 cut(s) 61, 550, 1040
MabI ACCWGGT 1 cut(s) 210
MaeI CTAG 6 cut(s) 252, 336, 354, 389, 719, 1299
MaeIII GTNAC 6 cut(s) 206, 254, 308, 506, 910, 1358
MalI GATC 2 cut(s) 516, 1234
MboI GATC 2 cut(s) 514, 1232
MboII GAAGA 5 cut(s) 516, 863, 896, 1076, 1407
MfeI CAATTG 2 cut(s) 66, 1409
MflI RGATCY 2 cut(s) 514, 1232
MhlI GDGCHC 2 cut(s) 457, 1109
MlsI TGGCCA 1 cut(s) 690
MluCI AATT 8 cut(s) 66, 127, 329, 375, 647, 890, 936, 1409
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 1342
MnlI CCTC 8 cut(s) 387, 435, 537, 600, 723, 962, 977, 1152
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 76
MscI TGGCCA 1 cut(s) 690
MseI TTAA 5 cut(s) 126, 660, 876, 893, 1501
MslI CAYNNNNRTG 2 cut(s) 960, 1167
Msp20I TGGCCA 1 cut(s) 690
MspR9I CCNGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
MunI CAATTG 2 cut(s) 66, 1409
MvaI CCWGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
MwoI GCNNNNNNNGC 2 cut(s) 687, 748
NcoI CCATGG 1 cut(s) 1257
NdeII GATC 2 cut(s) 514, 1232
NlaIV GGNNCC 5 cut(s) 6, 195, 325, 1234, 1337
NmuCI GTSAC 3 cut(s) 308, 910, 1358
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 1 cut(s) 983
PaqCI CACCTGC 1 cut(s) 497
PciSI GCTCTTC 1 cut(s) 529
PfeI GAWTC 9 cut(s) 140, 167, 248, 317, 365, 854, 1049, 1277, 1295
PflMI CCANNNNNTGG 1 cut(s) 217
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp124BI GAGCTC 2 cut(s) 457, 1109
Psp6I CCWGG 6 cut(s) 210, 360, 492, 829, 1068, 1338
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 6 cut(s) 210, 360, 492, 829, 1068, 1338
PspN4I GGNNCC 5 cut(s) 6, 195, 325, 1234, 1337
PspPI GGNCC 2 cut(s) 1336, 1388
PsuI RGATCY 2 cut(s) 514, 1232
RsaI GTAC 2 cut(s) 88, 536
RsaNI GTAC 2 cut(s) 87, 535
RseI CAYNNNNRTG 2 cut(s) 960, 1167
SacI GAGCTC 2 cut(s) 457, 1109
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 5 cut(s) 126, 660, 876, 893, 1501
Sau3AI GATC 2 cut(s) 514, 1232
Sau96I GGNCC 2 cut(s) 1336, 1388
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 6 cut(s) 212, 362, 494, 831, 1070, 1340
SduI GDGCHC 2 cut(s) 457, 1109
SexAI ACCWGGT 1 cut(s) 210
SfaNI GCATC 3 cut(s) 61, 550, 1040
SinI GGWCC 2 cut(s) 1336, 1388
SmiMI CAYNNNNRTG 2 cut(s) 960, 1167
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 8 cut(s) 66, 127, 329, 375, 647, 890, 936, 1409
SsiI CCGC 1 cut(s) 1493
SspMI CTAG 6 cut(s) 252, 336, 354, 389, 719, 1299
SstI GAGCTC 2 cut(s) 457, 1109
StyD4I CCNGG 6 cut(s) 210, 360, 492, 829, 1068, 1338
StyI CCWWGG 3 cut(s) 8, 1209, 1257
TaaI ACNGT 4 cut(s) 265, 902, 1201, 1487
TaqI TCGA 2 cut(s) 132, 651
TasI AATT 8 cut(s) 66, 127, 329, 375, 647, 890, 936, 1409
TatI WGTACW 1 cut(s) 86
TfiI GAWTC 9 cut(s) 140, 167, 248, 317, 365, 854, 1049, 1277, 1295
Tru1I TTAA 5 cut(s) 126, 660, 876, 893, 1501
Tru9I TTAA 5 cut(s) 126, 660, 876, 893, 1501
TseFI GTSAC 3 cut(s) 308, 910, 1358
Tsp45I GTSAC 3 cut(s) 308, 910, 1358
TspGWI ACGGA 1 cut(s) 327
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 2 cut(s) 1336, 1388
XagI CCTNNNNNAGG 1 cut(s) 999
XapI RAATTY 2 cut(s) 329, 647
XceI RCATGY 1 cut(s) 983
XcmI CCANNNNNNNNNTGG 1 cut(s) 1169
XmiI GTMKAC 1 cut(s) 676
XspI CTAG 6 cut(s) 252, 336, 354, 389, 719, 1299
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.