Rroxscaffold_5G00379060

Squamosa promoter-binding-like protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005916
Physical Location & Seq
Forward (+)
59563277 .. 59566889
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379060.1

Sequence Viewer

Length: 1611 bp
ATGGAGCCTTGGAGTTTCGGTTCAGAAGGGAAAGGGTTTTTACTTGCTGATGAGATGGATATACCAATTGATGCATTGGCAAGGAGTACAAAAGCATTCCTAGAATGGGATAACAAAACTTGCTTTAATTTCGATAAAGAATCAGTTGAGAGCATGGAGTTCATGGATTTGGGATTGCCAGATACAGTGAGGGAGCCTTGTCATGGTAACCAAGTAGTGGATGTCTTGAGTGGTGTTGCTGGTGTGGGCTGTAATGATTCTAGTAACAATACCGTAGCTTCTCCTACTTGTTTGATGACTTCAAGTTCATCTTGGGGTGAAGGAGAATCTGGCTCCAAATTTTCGAGTCCAGTCTGTGAGTCTAGTAGCCAGGATTCATCACTTATTGCTTTGAAGCTAGGGAGGTTGGCTGATAGCAAATATGGACAGGATAGTCAACATCTGAAAGAGAGGTCAAAGAGAGCTCGCACAAAGGGTCCACACTATCAGACTCTCACCTGCCAAGTTCATGGTTGTAACATGGATCTTAGCTCTTCAAAGGAGTACCATAAGAGGCATAGAGTTTGTGATGCTCACTCAAAGACTGCAATAGTTATCGTTAATGGCATTGAACAGAGGTTTCATCTGCTGGCTGAATTCGATGATGTTAAGCGCAGTTGTCGTAGACGCTTAGCTGGCCACAATCAACGCCGCAGGAAGCCTCAACTAGATACTTTTGATTGTAAATCACGTAAGTGGCTTCAGTCATATCAAGGCATTGGATATCTTGGAACTTCCTTGCCGAAGAGAACACCGTTTGTCTTTTCAGATATACTTCCAGGTGGCATTCTATATCCAGAAGAATCTAACGAGTATGGACATATTAAAACTGAAGATGAATCAACTTACAGTCAATGTGTGACACCCATGACAAACAGGCCATTACCTCCAAACTCTTTCCTCCAACTGCATGGCCTCGGGAAGCAACATGCTTCAGAAGTTCCTTCGTCAGGAAGTGAATTCTCGGTTTTTGATACTGCATCACATGTCCAGAAAGCATCTGGGGTGTCAACCTACCAGGGTGCTCTCTCTCTTCTGTCAGCTCAATCACAGAGCTCAATCCATTCAGCAAGAAATCAAGTGGCTACCCCTCTGCAAATACAGAGAACCCATCATGGTGCTGGTCAAATTAATGAACGGCCTTCCACAGTAAAAGCCTTGGAAAAATATGGACGAAACAGGTCCTTCCCATGCAGGATAAACTCCTTGGGAACTGACCAGATGGACTCAGTAATGATTTCTGATTCTAGTCATGCTGTTGACTTCCAACTTCACACAGATAGGGTTTTCCAGGAGTCAGCTCGCTTCAGTCACAAGTATCATCTTTCTCCTGAAAATGGACCAACTTTTGATTTGCTCCAATTGTCTTCACATTTTCAAAGAGTGGAGCAAGAGAGAAACTTTTGCAAGTCAAACATGAGCAGGGGGATTTCTACTGTTTCCCGCAATCTTAAGTTCATTCAAGTGACAATCTTGTTGGAGGCAGTTTGGATTGGCTTCATCAGGGAAAGTCTTAACTACATTGGTGCATTTCAGAGGCTGGTAGAAAACTTTTCTTTTCCGTTGAAGTAA

Protein Analysis

536

Amino Acids

59.9

Weight (kDa)

7.93

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 167 - 233 1.4e-21 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 506
AasI GACNNNNNNGTC 1 cut(s) 432
Acc36I ACCTGC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 217
AccI GTMKAC 1 cut(s) 664
AciI CCGC 2 cut(s) 691, 1483
AclWI GGATC 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 3 cut(s) 338, 635, 998
AcuI CTGAAG 4 cut(s) 725, 891, 957, 1330
AfaI GTAC 2 cut(s) 88, 545
AfiI CCNNNNNNNGG 5 cut(s) 106, 203, 217, 989, 1376
AflII CTTAAG 1 cut(s) 1490
AflIII ACRYGT 1 cut(s) 1024
AgsI TTSAA 7 cut(s) 303, 394, 537, 611, 1418, 1502, 1606
AjnI CCWGG 4 cut(s) 369, 817, 1056, 1329
AleI CACNNNNGTG 1 cut(s) 733
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 8 cut(s) 278, 397, 464, 531, 674, 1082, 1095, 1340
AluI AGCT 8 cut(s) 278, 397, 464, 531, 674, 1082, 1095, 1340
Alw21I GWGCWC 3 cut(s) 466, 1066, 1097
AlwI GGATC 1 cut(s) 531
AlwNI CAGNNNCTG 1 cut(s) 1579
Ama87I CYCGRG 1 cut(s) 956
AoxI GGCC 4 cut(s) 676, 917, 952, 1178
ApoI RAATTY 3 cut(s) 338, 635, 998
AseI ATTAAT 1 cut(s) 1170
AspLEI GCGC 1 cut(s) 654
AspS9I GGNCC 3 cut(s) 476, 1221, 1379
AsuHPI GGTGA 2 cut(s) 329, 487
AvaI CYCGRG 1 cut(s) 956
AvaII GGWCC 3 cut(s) 476, 1221, 1379
BalI TGGCCA 1 cut(s) 678
BanII GRGCYC 2 cut(s) 466, 1097
BbsI GAAGAC 1 cut(s) 1398
Bbv12I GWGCWC 3 cut(s) 466, 1066, 1097
BccI CCATC 3 cut(s) 49, 1158, 1255
BceAI ACGGC 1 cut(s) 1193
BciT130I CCWGG 4 cut(s) 371, 819, 1058, 1331
BfaI CTAG 6 cut(s) 101, 261, 363, 398, 707, 1287
BfrI CTTAAG 1 cut(s) 1490
BfuAI ACCTGC 1 cut(s) 506
BisI GCNGC 1 cut(s) 691
BlpI GCTNAGC 1 cut(s) 670
BlsI GCNGC 1 cut(s) 692
Bme1390I CCNGG 4 cut(s) 371, 819, 1058, 1331
Bme18I GGWCC 3 cut(s) 476, 1221, 1379
BmeT110I CYCGRG 1 cut(s) 956
BmgT120I GGNCC 3 cut(s) 476, 1221, 1379
BmiI GGNNCC 4 cut(s) 6, 195, 334, 477
BmrFI CCNGG 4 cut(s) 371, 819, 1058, 1331
BmsI GCATC 4 cut(s) 61, 559, 1028, 1046
BpiI GAAGAC 1 cut(s) 1398
Bpu1102I GCTNAGC 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 247
BsaAI YACGTR 1 cut(s) 731
BsaJI CCNNGG 5 cut(s) 8, 955, 1057, 1197, 1245
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 203, 217, 989, 1376
Bse1I ACTGG 1 cut(s) 350
BseBI CCWGG 4 cut(s) 371, 819, 1058, 1331
BseDI CCNNGG 5 cut(s) 8, 955, 1057, 1197, 1245
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 5 cut(s) 106, 203, 217, 989, 1376
BseMII CTCAG 1 cut(s) 1281
BseNI ACTGG 1 cut(s) 350
BshFI GGCC 4 cut(s) 678, 919, 954, 1180
BsiHKAI GWGCWC 3 cut(s) 466, 1066, 1097
BsiHKCI CYCGRG 1 cut(s) 956
BslI CCNNNNNNNGG 5 cut(s) 106, 203, 217, 989, 1376
BsmI GAATGC 2 cut(s) 95, 825
BsnI GGCC 4 cut(s) 678, 919, 954, 1180
BsoBI CYCGRG 1 cut(s) 956
Bsp1286I GDGCHC 3 cut(s) 466, 1066, 1097
Bsp143I GATC 1 cut(s) 523
Bsp1720I GCTNAGC 1 cut(s) 670
BspACI CCGC 2 cut(s) 691, 1483
BspANI GGCC 4 cut(s) 678, 919, 954, 1180
BspCNI CTCAG 1 cut(s) 1280
BspLI GGNNCC 4 cut(s) 6, 195, 334, 477
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 1 cut(s) 531
BspQI GCTCTTC 1 cut(s) 538
BspTI CTTAAG 1 cut(s) 1490
BsrI ACTGG 1 cut(s) 350
BssECI CCNNGG 5 cut(s) 8, 955, 1057, 1197, 1245
BssMI GATC 1 cut(s) 523
BssT1I CCWWGG 3 cut(s) 8, 1197, 1245
Bst2UI CCWGG 4 cut(s) 371, 819, 1058, 1331
Bst4CI ACNGT 6 cut(s) 187, 274, 795, 890, 1189, 1477
Bst6I CTCTTC 3 cut(s) 538, 779, 1077
BstAFI CTTAAG 1 cut(s) 1490
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 4 cut(s) 466, 630, 676, 1342
BstDEI CTNAG 3 cut(s) 527, 670, 1267
BstEII GGTNACC 1 cut(s) 206
BstENI CCTNNNNNAGG 1 cut(s) 987
BstF5I GGATG 1 cut(s) 226
BstHHI GCGC 1 cut(s) 654
BstKTI GATC 1 cut(s) 526
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 1 cut(s) 675
BstNI CCWGG 4 cut(s) 371, 819, 1058, 1331
BstNSI RCATGY 2 cut(s) 971, 1028
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 4 cut(s) 369, 817, 1056, 1329
BstV2I GAAGAC 1 cut(s) 1398
BstX2I RGATCY 1 cut(s) 523
BstXI CCANNNNNNTGG 2 cut(s) 509, 950
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 4 cut(s) 678, 919, 954, 1180
BtsCI GGATG 1 cut(s) 226
BtsIMutI CAGTG 1 cut(s) 192
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 4 cut(s) 466, 630, 676, 1342
CaiI CAGNNNCTG 1 cut(s) 1579
CfoI GCGC 1 cut(s) 654
Cfr13I GGNCC 3 cut(s) 476, 1221, 1379
CseI GACGC 1 cut(s) 675
Csp6I GTAC 2 cut(s) 87, 544
CviQI GTAC 2 cut(s) 87, 544
DdeI CTNAG 3 cut(s) 527, 670, 1267
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
DrdI GACNNNNNNGTC 1 cut(s) 432
DseDI GACNNNNNNGTC 1 cut(s) 432
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 3 cut(s) 538, 779, 1077
EarI CTCTTC 3 cut(s) 538, 779, 1077
Ecl136II GAGCTC 2 cut(s) 464, 1095
Eco130I CCWWGG 3 cut(s) 8, 1197, 1245
Eco24I GRGCYC 2 cut(s) 466, 1097
Eco32I GATATC 1 cut(s) 764
Eco47I GGWCC 3 cut(s) 476, 1221, 1379
Eco53kI GAGCTC 2 cut(s) 464, 1095
Eco57I CTGAAG 4 cut(s) 725, 891, 957, 1330
Eco88I CYCGRG 1 cut(s) 956
Eco91I GGTNACC 1 cut(s) 206
EcoICRI GAGCTC 2 cut(s) 464, 1095
EcoNI CCTNNNNNAGG 1 cut(s) 987
EcoO109I RGGNCCY 1 cut(s) 1221
EcoO65I GGTNACC 1 cut(s) 206
EcoRI GAATTC 2 cut(s) 635, 998
EcoRII CCWGG 4 cut(s) 369, 817, 1056, 1329
EcoRV GATATC 1 cut(s) 764
EcoT14I CCWWGG 3 cut(s) 8, 1197, 1245
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 2 cut(s) 466, 1097
ErhI CCWWGG 3 cut(s) 8, 1197, 1245
FalI AAGNNNNNCTT 2 cut(s) 295, 327
FauI CCCGC 1 cut(s) 1490
FblI GTMKAC 1 cut(s) 664
Fnu4HI GCNGC 1 cut(s) 691
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 2 cut(s) 466, 1097
Fsp4HI GCNGC 1 cut(s) 691
FspBI CTAG 6 cut(s) 101, 261, 363, 398, 707, 1287
GlaI GCGC 1 cut(s) 653
GluI GCNGC 1 cut(s) 691
HaeIII GGCC 4 cut(s) 678, 919, 954, 1180
HgaI GACGC 1 cut(s) 675
HhaI GCGC 1 cut(s) 654
Hin6I GCGC 1 cut(s) 652
HinP1I GCGC 1 cut(s) 652
HincII GTYRAC 3 cut(s) 437, 1050, 1300
HindII GTYRAC 3 cut(s) 437, 1050, 1300
HphI GGTGA 2 cut(s) 329, 487
Hpy166II GTNNAC 5 cut(s) 437, 479, 665, 1050, 1300
Hpy188I TCNGA 7 cut(s) 25, 444, 489, 808, 976, 1282, 1575
Hpy188III TCNNGA 6 cut(s) 226, 836, 958, 990, 1030, 1370
Hpy8I GTNNAC 5 cut(s) 437, 479, 665, 1050, 1300
HpyAV CCTTC 5 cut(s) 20, 314, 993, 1191, 1234
HpyCH4III ACNGT 6 cut(s) 187, 274, 795, 890, 1189, 1477
HpyCH4IV ACGT 1 cut(s) 730
HpyCH4V TGCA 8 cut(s) 74, 587, 949, 1019, 1135, 1233, 1446, 1568
HpyF10VI GCNNNNNNNGC 1 cut(s) 675
HpyF3I CTNAG 3 cut(s) 527, 670, 1267
HpySE526I ACGT 1 cut(s) 730
HspAI GCGC 1 cut(s) 652
Kzo9I GATC 1 cut(s) 523
LguI GCTCTTC 1 cut(s) 538
LmnI GCTCC 5 cut(s) 4, 193, 338, 1401, 1426
LweI GCATC 4 cut(s) 61, 559, 1028, 1046
MaeI CTAG 6 cut(s) 101, 261, 363, 398, 707, 1287
MaeII ACGT 1 cut(s) 730
MaeIII GTNAC 6 cut(s) 206, 263, 515, 898, 1349, 1504
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 6 cut(s) 525, 796, 851, 884, 1064, 1398
MfeI CAATTG 2 cut(s) 66, 1400
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 3 cut(s) 466, 1066, 1097
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 7 cut(s) 66, 127, 338, 635, 998, 1167, 1400
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 5 cut(s) 355, 368, 484, 1259, 1343
MmeI TCCRAC 3 cut(s) 967, 1330, 1497
Mox20I TGGCCA 1 cut(s) 678
Mph1103I ATGCAT 1 cut(s) 76
MscI TGGCCA 1 cut(s) 678
MseI TTAA 7 cut(s) 126, 600, 648, 864, 1170, 1491, 1554
MslI CAYNNNNRTG 3 cut(s) 733, 1155, 1502
Msp20I TGGCCA 1 cut(s) 678
MspCI CTTAAG 1 cut(s) 1490
MspR9I CCNGG 4 cut(s) 371, 819, 1058, 1331
MunI CAATTG 2 cut(s) 66, 1400
Mva1269I GAATGC 2 cut(s) 95, 825
MvaI CCWGG 4 cut(s) 371, 819, 1058, 1331
MwoI GCNNNNNNNGC 1 cut(s) 675
NdeII GATC 1 cut(s) 523
NlaIV GGNNCC 4 cut(s) 6, 195, 334, 477
NmuCI GTSAC 3 cut(s) 898, 1349, 1504
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 2 cut(s) 971, 1028
OliI CACNNNNGTG 1 cut(s) 733
PaqCI CACCTGC 1 cut(s) 506
PciI ACATGT 1 cut(s) 1024
PciSI GCTCTTC 1 cut(s) 538
PctI GAATGC 2 cut(s) 95, 825
PfeI GAWTC 7 cut(s) 140, 257, 326, 374, 842, 878, 1283
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 1329
PkrI GCNGC 1 cut(s) 692
PleI GAGTC 5 cut(s) 354, 367, 484, 1259, 1342
PpsI GAGTC 5 cut(s) 354, 367, 484, 1259, 1342
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 1221
PscI ACATGT 1 cut(s) 1024
PshBI ATTAAT 1 cut(s) 1170
Psp124BI GAGCTC 2 cut(s) 466, 1097
Psp5II RGGWCCY 1 cut(s) 1221
Psp6I CCWGG 4 cut(s) 369, 817, 1056, 1329
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 4 cut(s) 369, 817, 1056, 1329
PspN4I GGNNCC 4 cut(s) 6, 195, 334, 477
PspPI GGNCC 3 cut(s) 476, 1221, 1379
PspPPI RGGWCCY 1 cut(s) 1221
PstNI CAGNNNCTG 1 cut(s) 1579
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 2 cut(s) 88, 545
RsaNI GTAC 2 cut(s) 87, 544
RseI CAYNNNNRTG 3 cut(s) 733, 1155, 1502
SacI GAGCTC 2 cut(s) 466, 1097
SapI GCTCTTC 1 cut(s) 538
SaqAI TTAA 7 cut(s) 126, 600, 648, 864, 1170, 1491, 1554
SatI GCNGC 1 cut(s) 691
Sau3AI GATC 1 cut(s) 523
Sau96I GGNCC 3 cut(s) 476, 1221, 1379
SchI GAGTC 5 cut(s) 355, 368, 484, 1259, 1343
ScrFI CCNGG 4 cut(s) 371, 819, 1058, 1331
SduI GDGCHC 3 cut(s) 466, 1066, 1097
SfaNI GCATC 4 cut(s) 61, 559, 1028, 1046
SinI GGWCC 3 cut(s) 476, 1221, 1379
SmiMI CAYNNNNRTG 3 cut(s) 733, 1155, 1502
SmlI CTYRAG 2 cut(s) 226, 1490
SmoI CTYRAG 2 cut(s) 226, 1490
Sse9I AATT 7 cut(s) 66, 127, 338, 635, 998, 1167, 1400
SsiI CCGC 2 cut(s) 691, 1483
SspMI CTAG 6 cut(s) 101, 261, 363, 398, 707, 1287
SstI GAGCTC 2 cut(s) 466, 1097
StyD4I CCNGG 4 cut(s) 369, 817, 1056, 1329
StyI CCWWGG 3 cut(s) 8, 1197, 1245
TaaI ACNGT 6 cut(s) 187, 274, 795, 890, 1189, 1477
TaiI ACGT 1 cut(s) 733
TaqI TCGA 3 cut(s) 132, 344, 639
TasI AATT 7 cut(s) 66, 127, 338, 635, 998, 1167, 1400
TatI WGTACW 1 cut(s) 86
TauI GCSGC 1 cut(s) 693
TfiI GAWTC 7 cut(s) 140, 257, 326, 374, 842, 878, 1283
Tru1I TTAA 7 cut(s) 126, 600, 648, 864, 1170, 1491, 1554
Tru9I TTAA 7 cut(s) 126, 600, 648, 864, 1170, 1491, 1554
TscAI CASTG 1 cut(s) 192
TseFI GTSAC 3 cut(s) 898, 1349, 1504
Tsp45I GTSAC 3 cut(s) 898, 1349, 1504
TspDTI ATGAA 9 cut(s) 151, 297, 366, 497, 611, 891, 1188, 1486, 1528
TspGWI ACGGA 1 cut(s) 1590
TspRI CASTG 1 cut(s) 192
Van91I CCANNNNNTGG 1 cut(s) 217
Vha464I CTTAAG 1 cut(s) 1490
VpaK11BI GGWCC 3 cut(s) 476, 1221, 1379
VspI ATTAAT 1 cut(s) 1170
XagI CCTNNNNNAGG 1 cut(s) 987
XapI RAATTY 3 cut(s) 338, 635, 998
XceI RCATGY 2 cut(s) 971, 1028
XcmI CCANNNNNNNNNTGG 2 cut(s) 1037, 1157
XmiI GTMKAC 1 cut(s) 664
XspI CTAG 6 cut(s) 101, 261, 363, 398, 707, 1287
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.